Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?pN1 Generation Definitions   Donating Investigator Dr. Thomas C. Sudhof,   Stanford University School of Medicine

Description
This targeted mutation strain carries a deletion of exons 2 and 3 of the gene. These mice show no alteration in their inhibitory synaptic transmission characteristics. No protein product is detected by Western blot analysis of homozygous brain extracts. Homozygotes are viable, normal in size and do not display any gross physical abnormalities. This mutant mouse strain may be useful in studies of synapse formation and/or function.

Development
Exons 2 and 3 were deleted using Cre-lox and FLP-FRT technologies. The targeting vector was introduced to (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. This line was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.

Related Strains

Strains carrying other alleles of Nlgn3

008475	B6;129-Nlgn3tm1Sud/J
015835	STOCK Nlgn3tm2Sud/J
015834	STOCK Nlgn3tm3.1Sud/J

View Strains carrying other alleles of Nlgn3     (3 strains)

Additional Web Information

JAX^® NOTES, Winter 2008; 512. JAX^® Mice strain sheds new light on autism.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Asperger Syndrome, X-Linked, Susceptibility to, 1; ASPGX1   (NLGN3) Autism   (NLGN3) Autism, Susceptibility to, X-Linked 1; AUTSX1   (NLGN3)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Nlgn3^tm2.1Sud/Y

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• normal phenotype
• no abnormal phenotype detected
  • mice are viable, fertile, have no obvious abnormalities, and exhibit normal neurological and behavioral phenotypes   (MGI Ref ID J:125344)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Neurodevelopmental Defects

Neurobiology Research
Neurodevelopmental Defects
      Autism

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Nlgn3tm2.1Sud
Allele Name		targeted mutation 2.1, Thomas Sudhof
Allele Type		Targeted (knock-out)
Mutation Made By		Dr. Thomas Sudhof,   Stanford University School of Medicine
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Nlgn3, neuroligin 3
Chromosome		X
Gene Common Name(s)		A230085M13Rik; HNL3; NL3; RIKEN cDNA A230085M13 gene;
Molecular Note		Exons 2 and 3 were removed by cre-mediated recombination that also removed the neo cassette. The loss of protein expression was confirmed by western blot analysis using two antibodies (528B and 639B). [MGI Ref ID J:125344]

Genotyping

Genotyping Information

Genotyping Protocols

Nlgn3^tm2.1Sud, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Tabuchi K; Blundell J; Etherton MR; Hammer RE; Liu X; Powell CM; Sudhof TC. 2007. A neuroligin-3 mutation implicated in autism increases inhibitory synaptic transmission in mice. Science 318(5847):71-6. [PubMed: 17823315]  [MGI Ref ID J:125344]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintained as a live colony, homozygous females, heterozygous females or hemizygous males may be bred. This gene/mutation is X-linked.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

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