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| These "CT alpha flox" mutant mice carry a loxP site-flanked allele of phosphate cytidylyltransferase 1, choline, alpha isoform (Pcyt1a) and may be useful in generating conditional mutations for studying atherosclerosis. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N5+F3pN1
Generation DefinitionsDonating Investigator Ira Tabas, Columbia University Description
These mice possess loxP sites on either side of exons 4 and 5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4 and 5 deleted in the cre-expressing tissue(s).When bred to a strain with inducible Cre recombinase expression in the myeloid cell lineage (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of atherosclerosis.
Development
A loxP site flanked neo cassette and a thymidine kinase gene was inserted downstream of exon 5 of the targeted gene, and another loxP site was inserted upstream of exon 4. This construct was electroporated into 129/Sv derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Resulting chimeric male animals were backcrossed to wild-type C57BL/6J mice. Heterozygotes were interbred to produce homozygotes. Mice homozygous for the floxed allele were then bred to B6.129P2-Lyz2tm1(cre)Ifo/J (Stock No. 004781) mice and backcrossed to C57BL/6J for four generations. Upon arrival at The Jackson Laboratory the mice were bred to C57BL/6J to remove the Lyz2tm1(cre)Ifo allele. Heterozygotes were crossed to generate homozygotes.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Lyz2tm1(cre)Ifo/Lyz2+ Pcyt1atm1Irt/Pcyt1atm1Irt
involves: 129/Sv * C57BL/6J (conditional)
- immune system phenotype
- abnormal immune cell physiology (MGI Ref ID J:65639)
- abnormal macrophage physiology
- normal numbers of peritoneal macrophage (MGI Ref ID J:65639)
- no CTP:phosphocholine cytidylyltransferase alpha produced (MGI Ref ID J:65639)
- CTP:phosphocholine cytidylyltransferase, beta isoform upregulated (MGI Ref ID J:65639)
- severely reduced conversion of free cholesterol to phosphatidylcholine (MGI Ref ID J:65639)
- increased free cholesterol load leads to macrophage cell death (MGI Ref ID J:65639)
This mouse can be used to support research in many areas including:
Research Tools
Cardiovascular Research
Cre-lox System
Cre-lox System
loxP-flanked Sequences
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Pcyt1atm1Irt | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Ira Tabas | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | CT alpha flox; | ||
| Mutation Made By | Ira Tabas, Columbia University | ||
| Strain of Origin | 129/Sv | ||
| Gene Symbol and Name | Pcyt1a, phosphate cytidylyltransferase 1, choline, alpha isoform | ||
| Chromosome | 16 | ||
| Gene Common Name(s) | CCT-alpha; CCTA; CT; CTA; CTP; CTP phosphocholine cytidylyl transferase; CTP:phosphocholine cytidylyltransferase alpha; CTPCT; CTalpha; Cctalpha; Ctpct; Cttalpha; PCYT1; | ||
| Molecular Note | A loxP site was inserted 330 bp upstream of exon 4. At the same time, a floxed neomycin cassette was inserted in intron 5. [MGI Ref ID J:65639] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Pcyt1atm1Irt, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Zhang D; Tang W; Yao PM; Yang C; Xie B; Jackowski S; Tabas I. 2000. Macrophages deficient in CTP:Phosphocholine cytidylyltransferase-alpha are viable under normal culture conditions but are highly susceptible to free cholesterol-induced death. MOLECULAR GENETIC EVIDENCE THAT THE INDUCTION OF PHOSPHATIDYLCHOLINE BIOSYNTHESIS IN FREE CHOLESTEROL-LOADED MACROPHAGES IS AN ADAPTIVE RESPONSE [In Process Citation] J Biol Chem 275(45):35368-76. [PubMed: 10944538] [MGI Ref ID J:65639]
Additional References
Pcyt1atm1Irt relatedFagone P; Gunter C; Sage CR; Gunn KE; Brewer JW; Jackowski S. 2009. CTP:phosphocholine cytidylyltransferase alpha is required for B-cell proliferation and class switch recombination. J Biol Chem 284(11):6847-54. [PubMed: 19139091] [MGI Ref ID J:148370]
Jacobs RL; Devlin C; Tabas I; Vance DE. 2004. Targeted deletion of hepatic CTP:phosphocholine cytidylyltransferase alpha in mice decreases plasma high density and very low density lipoproteins. J Biol Chem 279(45):47402-10. [PubMed: 15331603] [MGI Ref ID J:94481]
Jacobs RL; Lingrell S; Zhao Y; Francis GA; Vance DE. 2008. Hepatic CTP:phosphocholine cytidylyltransferase-alpha is a critical predictor of plasma high density lipoprotein and very low density lipoprotein. J Biol Chem 283(4):2147-55. [PubMed: 18042552] [MGI Ref ID J:130719]
Jacobs RL; Stead LM; Devlin C; Tabas I; Brosnan ME; Brosnan JT; Vance DE. 2005. Physiological regulation of phospholipid methylation alters plasma homocysteine in mice. J Biol Chem 280(31):28299-305. [PubMed: 15958390] [MGI Ref ID J:107131]
Tian Y; Zhou R; Rehg JE; Jackowski S. 2007. Role of phosphocholine cytidylyltransferase alpha in lung development. Mol Cell Biol 27(3):975-82. [PubMed: 17130238] [MGI Ref ID J:118292]
Wang L; Magdaleno S; Tabas I; Jackowski S. 2005. Early embryonic lethality in mice with targeted deletion of the CTP:phosphocholine cytidylyltransferase alpha gene (Pcyt1a). Mol Cell Biol 25(8):3357-63. [PubMed: 15798219] [MGI Ref ID J:97657]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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