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| These transgenic mice express the human tumor-associated calcium signal transducer 1 gene (TACSTD1). Transgene expression pattern is similar to the observed human pattern in both normal and tumor tissue. This mutant mouse strain may be useful in studies of immunological therapies for cancer. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N8pN1
Generation DefinitionsDonating Investigator Lou de Leij, University Hospital Groningen Description
These transgenic mice express the human tumor-associated calcium signal transducer 1 gene.Transgene expression pattern is similar to the observed human pattern in both normal and tumor tissue.
Founder line 02 (BACF2) carries approximately 3 copies of the transgene.
Mice that are hemizygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of immunological therapies for cancer.
Development
A transgenic construct containing the human tumor-associated calcium signal transducer 1 gene (TACSTD1), isolated from a BAC clone, was injected into fertilized FVB/NHsd mouse eggs. Founder line 02 (BACF2) was subsequently established. The mice were backcrossed to C57BL/6 (Harlan) for eight generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(TACSTD1)02Leij/0
involves: C57BL/6 * FVB/N
- normal phenotype
- no abnormal phenotype detected
- hemizygotes are viable, fertile, and have normal lifespan; mice do not show morphological aberrations and do not develop neoplastic lesions (MGI Ref ID J:69638)
This mouse can be used to support research in many areas including:
Research Tools
Cancer Research
tumor immunology
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(TACSTD1)02Leij | ||
|---|---|---|---|
| Allele Name | transgene insertion 02, Prof Lou de Leij | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | BACF2; EGP-2 tg; Hu-EpCAM; TgN(EGP2BAC)2; hEpCAM Tg; | ||
| Mutation Made By | Marcel Ruiters, Synvolux therapeutics BV | ||
| Strain of Origin | FVB/NHsd | ||
| Expressed Gene | EPCAM, epithelial cell adhesion molecule, human | ||
| Promoter | EPCAM, epithelial cell adhesion molecule, human | ||
| Molecular Note | A transgenic construct containing the human tumor-associated calcium signal transducer 1 gene (TACSTD1), isolated from a BAC clone, was injected into fertilized FVB/NHsd mouse eggs. Founder line 02 (BACF2) was subsequently established. Transgene expression is directed to the membrane of epithelial cells and is detected in various epithelia in kidney, airways, bile ducts, GI tract (epithelium of villi and crypts of small and large intestine) and in glandular epithelium of reproductive system (epithelium of endometrium, testis tubuli seminifer and epithelium of fallopian ducts), as well as in Hassall's corpuscles in the thymus. Pattern is similar to human expression patterns in normal and tumor tissues. [MGI Ref ID J:69638] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(TACSTD1)02Leij, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
McLaughlin PM; Harmsen MC; Dokter WH; Kroesen BJ; van der Molen H; Brinker MG; Hollema H; Ruiters MH; Buys CH; de Leij LF. 2001. The epithelial glycoprotein 2 (EGP-2) promoter-driven epithelial-specific expression of EGP-2 in transgenic mice: a new model to study carcinoma-directed immunotherapy. Cancer Res 61(10):4105-11. [PubMed: 11358833] [MGI Ref ID J:69638]
Additional References
Tg(TACSTD1)02Leij relatedKosterink JG; McLaughlin PM; Lub-de Hooge MN; Hendrikse HH; van Zanten J; van Garderen E; Harmsen MC; de Leij LF. 2007. Biodistribution studies of epithelial cell adhesion molecule (EpCAM)-directed monoclonal antibodies in the EpCAM-transgenic mouse tumor model. J Immunol 179(2):1362-8. [PubMed: 17617629] [MGI Ref ID J:137741]
Trzpis M; McLaughlin PM; Popa ER; Terpstra P; van Kooten TG; de Leij LM; Harmsen MC. 2008. EpCAM homologues exhibit epithelial-specific but different expression patterns in the kidney. Transgenic Res 17(2):229-38. [PubMed: 17940847] [MGI Ref ID J:137740]
Trzpis M; McLaughlin PM; van Goor H; Brinker MG; van Dam GM; de Leij LM; Popa ER; Harmsen MC. 2008. Expression of EpCAM is up-regulated during regeneration of renal epithelia. J Pathol 216(2):201-8. [PubMed: 18702175] [MGI Ref ID J:140582]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator has not attempted to make the strain homozygous.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| fax: | 207-288-6655 |
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