Strain Name:

C.129S2(B6)-Selptm1Hyn/J

Stock Number:

008433

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Homozygous targeted mutant selectin, platelet (Selp) mice show a deficit in leukocyte rolling and delayed neutrophil extravasation in response to intraperitoneal injections of thioglycollate. Neutrophil count is elevated (two- to three-fold). Circulating lymphocyte and monocyte counts are normal. It can be used as model for human leukocyte adhesion deficiencies (characterized by elevated levels of circulating leukocytes and impaired leukocyte extravasation to sites of inflammation or infection).

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating InvestigatorDr. Richard Hynes,   Massachusetts Institute of Technology

Description
Mice homozygous for the Selptm1Hyn targeted mutation are viable and fertile. Homozygous mutant mice show a deficit in leukocyte rolling and delayed neutrophil extravasation in response to intraperitoneal injections of thioglycollate. Neutrophil count is elevated (two- to three-fold). Circulating lymphocyte and monocyte counts are normal. It can be used as model for human leukocyte adhesion deficiencies (characterized by elevated levels of circulating leukocytes and impaired leukocyte extravasation to sites of inflammation or infection).

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
A portion of exon 3 encoding 10 amino acids of a signal peptide and 27 amino acids of the lectin domain was deleted by the insertion of PGK-neo cassette. This mutation was created in 129S2/SvPas-derived D3 embryonic stem (ES) cells. This line has been backcrossed from a mixed C57BL/6 and 129P2 background to BALB/c for more than 10 generations.

Control Information

  Control
   001026 BALB/cByJ
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying   Selptm1Hyn     (11 strains)

Strains carrying other alleles of Selp
002285   B6.129S7-Icam1tm1Bay Selptm1Bay/J
002289   B6.129S7-Selptm1Bay/J
View Strains carrying other alleles of Selp     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
IgE Responsiveness, Atopic; IGER   (SELP)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Selptm1Hyn/Selptm1Hyn

        involves: 129S2/SvPas * C57BL/6J
  • immune system phenotype
  • abnormal leukocyte tethering or rolling
    • exhibit virtually total absence of leukocyte rolling in mesenteric venules   (MGI Ref ID J:77329)
    • two secretagogues (the calcium ionophore A23187 and hydrogen peroxide) that result in increased rolling in wild-type fail to stimulate any significant rolling in homozygotes   (MGI Ref ID J:77329)
  • impaired neutrophil recruitment
    • exhibit delayed recruitment of neutrophils to the peritoneal cavity upon experimentally induced inflammation   (MGI Ref ID J:77329)
  • increased neutrophil cell number
    • exhibit a 2.4-fold increase in circulating neutrophil counts   (MGI Ref ID J:77329)
  • hematopoietic system phenotype
  • abnormal leukocyte tethering or rolling
    • exhibit virtually total absence of leukocyte rolling in mesenteric venules   (MGI Ref ID J:77329)
    • two secretagogues (the calcium ionophore A23187 and hydrogen peroxide) that result in increased rolling in wild-type fail to stimulate any significant rolling in homozygotes   (MGI Ref ID J:77329)
  • impaired neutrophil recruitment
    • exhibit delayed recruitment of neutrophils to the peritoneal cavity upon experimentally induced inflammation   (MGI Ref ID J:77329)
  • increased neutrophil cell number
    • exhibit a 2.4-fold increase in circulating neutrophil counts   (MGI Ref ID J:77329)
  • homeostasis/metabolism phenotype
  • increased bleeding time
    • exhibit a 40% prolongation of the bleeding time on amputation of the tip of the tail   (MGI Ref ID J:31431)
  • cardiovascular system phenotype
  • skin hemorrhage
    • hemorrhagic skin lesions induced by injection of lipopolysaccharide followed by tumor necrosis factor are larger by a factor of two compared to controls   (MGI Ref ID J:31431)
  • cellular phenotype
  • abnormal leukocyte tethering or rolling
    • exhibit virtually total absence of leukocyte rolling in mesenteric venules   (MGI Ref ID J:77329)
    • two secretagogues (the calcium ionophore A23187 and hydrogen peroxide) that result in increased rolling in wild-type fail to stimulate any significant rolling in homozygotes   (MGI Ref ID J:77329)
  • integument phenotype
  • skin hemorrhage
    • hemorrhagic skin lesions induced by injection of lipopolysaccharide followed by tumor necrosis factor are larger by a factor of two compared to controls   (MGI Ref ID J:31431)

Selptm1Hyn/Selptm1Hyn

        involves: 129S2/SvPas
  • immune system phenotype
  • abnormal leukocyte tethering or rolling
    • rolling of leukocytes in subcutaneous vessels is significantly lower under both basal conditions and post-ischemia-reperfusion, but not completely eliminated   (MGI Ref ID J:29814)
    • leukocyte rolling is reduced even four hours after challenge; exhibit significantly fewer rolling cells and their velocity is reduced   (MGI Ref ID J:29814)
  • impaired macrophage chemotaxis
    • macrophage recruitment in a peritonitis model is reduced even 48 hours after challenge   (MGI Ref ID J:29814)
  • increased neutrophil cell number
    • increased numbers of circulating neutrophils   (MGI Ref ID J:29814)
    • clearing of neutrophils from the circulation is delayed in the first 2 to 3 hours after neutrophil injection, but is normal by 5 hours   (MGI Ref ID J:29814)
  • hematopoietic system phenotype
  • abnormal leukocyte tethering or rolling
    • rolling of leukocytes in subcutaneous vessels is significantly lower under both basal conditions and post-ischemia-reperfusion, but not completely eliminated   (MGI Ref ID J:29814)
    • leukocyte rolling is reduced even four hours after challenge; exhibit significantly fewer rolling cells and their velocity is reduced   (MGI Ref ID J:29814)
  • impaired macrophage chemotaxis
    • macrophage recruitment in a peritonitis model is reduced even 48 hours after challenge   (MGI Ref ID J:29814)
  • increased neutrophil cell number
    • increased numbers of circulating neutrophils   (MGI Ref ID J:29814)
    • clearing of neutrophils from the circulation is delayed in the first 2 to 3 hours after neutrophil injection, but is normal by 5 hours   (MGI Ref ID J:29814)
  • cellular phenotype
  • abnormal leukocyte tethering or rolling
    • rolling of leukocytes in subcutaneous vessels is significantly lower under both basal conditions and post-ischemia-reperfusion, but not completely eliminated   (MGI Ref ID J:29814)
    • leukocyte rolling is reduced even four hours after challenge; exhibit significantly fewer rolling cells and their velocity is reduced   (MGI Ref ID J:29814)
  • impaired macrophage chemotaxis
    • macrophage recruitment in a peritonitis model is reduced even 48 hours after challenge   (MGI Ref ID J:29814)

Selptm1Hyn/Selptm1Hyn

        involves: 129S2/SvPas * C57BL/6
  • immune system phenotype
  • abnormal leukocyte tethering or rolling
    • there is a third decrease in the number of rolling leukocytes within the mesenteric venules of mice treated with TNF   (MGI Ref ID J:57973)
    • there is also a third decrease in the number of adhering leukocytes within these mesenteric venules   (MGI Ref ID J:57973)
    • mean velocity of leukocytes is decreased   (MGI Ref ID J:57973)
  • impaired eosinophil recruitment
    • 2-fold fewer eosinophils are recruited to the peritoneum in response to ragweed allergen challenge   (MGI Ref ID J:57973)
  • impaired neutrophil recruitment
    • neutrophil influx in thioglycollate-induced peritonitis is inhibited at 2, 4, and 8 hours after injection   (MGI Ref ID J:57973)
  • increased eosinophil cell number
    • eosinophil numbers are about two-thirds higher in the blood   (MGI Ref ID J:57973)
  • increased neutrophil cell number
    • neutrophils numbers are slightly increased   (MGI Ref ID J:57973)
  • hematopoietic system phenotype
  • abnormal leukocyte tethering or rolling
    • there is a third decrease in the number of rolling leukocytes within the mesenteric venules of mice treated with TNF   (MGI Ref ID J:57973)
    • there is also a third decrease in the number of adhering leukocytes within these mesenteric venules   (MGI Ref ID J:57973)
    • mean velocity of leukocytes is decreased   (MGI Ref ID J:57973)
  • impaired eosinophil recruitment
    • 2-fold fewer eosinophils are recruited to the peritoneum in response to ragweed allergen challenge   (MGI Ref ID J:57973)
  • impaired neutrophil recruitment
    • neutrophil influx in thioglycollate-induced peritonitis is inhibited at 2, 4, and 8 hours after injection   (MGI Ref ID J:57973)
  • increased eosinophil cell number
    • eosinophil numbers are about two-thirds higher in the blood   (MGI Ref ID J:57973)
  • increased neutrophil cell number
    • neutrophils numbers are slightly increased   (MGI Ref ID J:57973)
  • cellular phenotype
  • abnormal leukocyte tethering or rolling
    • there is a third decrease in the number of rolling leukocytes within the mesenteric venules of mice treated with TNF   (MGI Ref ID J:57973)
    • there is also a third decrease in the number of adhering leukocytes within these mesenteric venules   (MGI Ref ID J:57973)
    • mean velocity of leukocytes is decreased   (MGI Ref ID J:57973)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Inflammation

Selptm1Hyn related

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Inflammation

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Selptm1Hyn
Allele Name targeted mutation 1, Richard Hynes
Allele Type Targeted (Null/Knockout)
Common Name(s) P-; P-Selp-; P-selectin-;
Mutation Made ByDr. Richard Hynes,   Massachusetts Institute of Technology
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Selp, selectin, platelet
Chromosome 1
Gene Common Name(s) CD62; CD62P; GMP140; GRMP; Grmp; LECAM3; P-selectin; PADGEM; PSEL; PSELECT; granulocyte membrane protein;
Molecular Note A portion of exon 3 encoding 10 amino acids of a signal peptide and 27 amino acids of the lectin domain was replaced with a PGK-neo cassette. Mice were treated with lipopolysaccharide to augment the normally low levels of selectin in lung and liver tissue. A longer transcript, resulting from aberrant or cryptic splicing involving the neomycin cassette, was detected at low levels in mutant mice by Northern blot analysis. RT-PCR further confirmed the presence of low levels of transcript in homozygous mice.Immunofluorescence analysis of activated platelets and lung sections from homozygous mutant mice showed an absence of encoded protein. These results were further confirmed by flow cytometric analysis of activated platelets and metabolic labeling of lung tissue. [MGI Ref ID J:77329]

Genotyping

Genotyping Information

Genotyping Protocols

Selptm1Hyn, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Mayadas TN; Johnson RC; Rayburn H; Hynes RO; Wagner DD. 1993. Leukocyte rolling and extravasation are severely compromised in P selectin-deficient mice. Cell 74(3):541-54. [PubMed: 7688665]  [MGI Ref ID J:77329]

Additional References

Selptm1Hyn related

Andre P; Denis CV; Ware J; Saffaripour S; Hynes RO; Ruggeri ZM; Wagner DD. 2000. Platelets adhere to and translocate on von willebrand factor presented by endothelium in stimulated veins Blood 96(10):3322-8. [PubMed: 11071623]  [MGI Ref ID J:65812]

Andre P; Hartwell D; Hrachovinova I; Saffaripour S; Wagner DD. 2000. Pro-coagulant state resulting from high levels of soluble P-selectin in blood. Proc Natl Acad Sci U S A 97(25):13835-40. [PubMed: 11095738]  [MGI Ref ID J:125020]

Belanger SD; St-Pierre Y. 2005. Role of selectins in the triggering, growth, and dissemination of T-lymphoma cells: implication of L-selectin in the growth of thymic lymphoma. Blood 105(12):4800-6. [PubMed: 15705798]  [MGI Ref ID J:107461]

Combes V; Rosenkranz AR; Redard M; Pizzolato G; Lepidi H; Vestweber D; Mayadas TN; Grau GE. 2004. Pathogenic role of P-selectin in experimental cerebral malaria: importance of the endothelial compartment. Am J Pathol 164(3):781-6. [PubMed: 14982832]  [MGI Ref ID J:88446]

Connolly ES Jr; Winfree CJ; Prestigiacomo CJ; Kim SC; Choudhri TF; Hoh BL; Naka Y; Solomon RA; Pinsky DJ. 1997. Exacerbation of cerebral injury in mice that express the P-selectin gene: identification of P-selectin blockade as a new target for the treatment of stroke. Circ Res 81(3):304-10. [PubMed: 9285631]  [MGI Ref ID J:43110]

Dole VS; Bergmeier W; Mitchell HA; Eichenberger SC; Wagner DD. 2005. Activated platelets induce Weibel-Palade-body secretion and leukocyte rolling in vivo: role of P-selectin. Blood 106(7):2334-9. [PubMed: 15956287]  [MGI Ref ID J:119376]

Doring A; Wild M; Vestweber D; Deutsch U; Engelhardt B. 2007. E- and P-selectin are not required for the development of experimental autoimmune encephalomyelitis in C57BL/6 and SJL mice. J Immunol 179(12):8470-9. [PubMed: 18056394]  [MGI Ref ID J:155033]

Egami K; Murohara T; Aoki M; Matsuishi T. 2006. Ischemia-induced angiogenesis: role of inflammatory response mediated by P-selectin. J Leukoc Biol 79(5):971-6. [PubMed: 16641139]  [MGI Ref ID J:108660]

Fernekorn U; Butcher EC; Behrends J; Hartz S; Kruse A. 2004. Functional involvement of P-selectin and MAdCAM-1 in the recruitment of alpha4beta7-integrin-expressing monocyte-like cells to the pregnant mouse uterus. Eur J Immunol 34(12):3423-33. [PubMed: 15484189]  [MGI Ref ID J:94597]

Fernekorn U; Butcher EC; Behrends J; Karsten CM; Robke A; Schulze TJ; Kirchner H; Kruse A. 2007. Selectin, platelet plays a critical role in granulocyte access to the pregnant mouse uterus under physiological and pathological conditions. Biol Reprod 76(4):645-53. [PubMed: 17151351]  [MGI Ref ID J:121141]

Forlow SB; White EJ; Barlow SC; Feldman SH; Lu H; Bagby GJ; Beaudet AL; Bullard DC; Ley K. 2000. Severe inflammatory defect and reduced viability in CD18 and E-selectin double-mutant mice J Clin Invest 106(12):1457-66. [PubMed: 11120753]  [MGI Ref ID J:66426]

Frederix K; Chauhan AK; Kisucka J; Zhao BQ; Hoff EI; Spronk HM; Ten Cate H; Wagner DD. 2007. Platelet adhesion receptors do not modulate infarct volume after a photochemically induced stroke in mice. Brain Res 1185:239-45. [PubMed: 17996853]  [MGI Ref ID J:130124]

Frenette J; Chbinou N; Godbout C; Marsolais D; Frenette PS. 2003. Macrophages, not neutrophils, infiltrate skeletal muscle in mice deficient in P/E selectins after mechanical reloading. Am J Physiol Regul Integr Comp Physiol 285(4):R727-32. [PubMed: 12829442]  [MGI Ref ID J:109393]

Frenette PS; Mayadas TN; Rayburn H; Hynes RO; Wagner DD. 1996. Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell 84(4):563-74. [PubMed: 8598043]  [MGI Ref ID J:31626]

Ghosh S; Chackerian AA; Parker CM; Ballantyne CM; Behar SM. 2006. The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection. J Immunol 176(8):4914-22. [PubMed: 16585587]  [MGI Ref ID J:131154]

Ginhoux F; Collin MP; Bogunovic M; Abel M; Leboeuf M; Helft J; Ochando J; Kissenpfennig A; Malissen B; Grisotto M; Snoeck H; Randolph G; Merad M. 2007. Blood-derived dermal langerin+ dendritic cells survey the skin in the steady state. J Exp Med 204(13):3133-46. [PubMed: 18086862]  [MGI Ref ID J:130817]

Gironella M; Molla M; Salas A; Soriano A; Sans M; Closa D; Engel P; Salas A; Pique JM; Panes J. 2002. The role of P-selectin in experimental colitis as determined by antibody immunoblockade and genetically deficient mice. J Leukoc Biol 72(1):56-64. [PubMed: 12101263]  [MGI Ref ID J:124457]

Goerge T; Ho-Tin-Noe B; Carbo C; Benarafa C; Remold-O'Donnell E; Zhao BQ; Cifuni SM; Wagner DD. 2008. Inflammation induces hemorrhage in thrombocytopenia. Blood 111(10):4958-64. [PubMed: 18256319]  [MGI Ref ID J:135316]

Hara T; Shimizu K; Ogawa F; Yanaba K; Iwata Y; Muroi E; Takenaka M; Komura K; Hasegawa M; Fujimoto M; Sato S. 2010. Platelets control leukocyte recruitment in a murine model of cutaneous arthus reaction. Am J Pathol 176(1):259-69. [PubMed: 20008131]  [MGI Ref ID J:156487]

Hidalgo A; Chang J; Jang JE; Peired AJ; Chiang EY; Frenette PS. 2009. Heterotypic interactions enabled by polarized neutrophil microdomains mediate thromboinflammatory injury. Nat Med 15(4):384-91. [PubMed: 19305412]  [MGI Ref ID J:149370]

Hirata T; Furie BC; Furie B. 2002. P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin. J Immunol 169(8):4307-13. [PubMed: 12370362]  [MGI Ref ID J:107382]

Homeister JW; Zhang M; Frenette PS; Hynes RO; Wagner DD; Lowe JB; Marks RM. 1998. Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation. Blood 92(7):2345-52. [PubMed: 9746773]  [MGI Ref ID J:50212]

Horie Y; Wolf R; Anderson DC; Granger DN. 1997. Hepatic leukostasis and hypoxic stress in adhesion molecule-deficient mice after gut ischemia/reperfusion. J Clin Invest 99(4):781-8. [PubMed: 9045883]  [MGI Ref ID J:39161]

Huang HS; Sun DS; Lien TS; Chang HH. 2010. Dendritic cells modulate platelet activity in IVIg-mediated amelioration of ITP in mice. Blood 116(23):5002-9. [PubMed: 20699442]  [MGI Ref ID J:167263]

Johnson RC; Mayadas TN; Frenette PS; Mebius RE; Subramaniam M; Lacasce A; Hynes RO; Wagner DD. 1995. Blood cell dynamics in P-selectin-deficient mice. Blood 86(3):1106-14. [PubMed: 7542495]  [MGI Ref ID J:28190]

Kaifi JT; Hall LR; Diaz C; Sypek J; Diaconu E; Lass JH; Pearlman E. 2000. Impaired eosinophil recruitment to the cornea in P-selectin-deficient mice in onchocerca volvulus keratitis (River blindness) Invest Ophthalmol Vis Sci 41(12):3856-61. [PubMed: 11053286]  [MGI Ref ID J:65551]

Karsten CM; Behrends J; Wagner AK; Fuchs F; Figge J; Schmudde I; Hellberg L; Kruse A. 2009. DC within the pregnant mouse uterus influence growth and functional properties of uterine NK cells. Eur J Immunol 39(8):2203-14. [PubMed: 19593769]  [MGI Ref ID J:151694]

Lu SX; Holland AM; Na IK; Terwey TH; Alpdogan O; Bautista JL; Smith OM; Suh D; King C; Kochman A; Hubbard VM; Rao UK; Yim N; Liu C; Laga AC; Murphy G; Jenq RR; Zakrzewski JL; Penack O; Dykstra L; Bampoe K; Perez L; Furie B; Furie B; van den Brink MR. 2010. Absence of P-selectin in recipients of allogeneic bone marrow transplantation ameliorates experimental graft-versus-host disease. J Immunol 185(3):1912-9. [PubMed: 20622117]  [MGI Ref ID J:162237]

Massaguer A; Perez-Del-Pulgar S; Engel P; Serratosa J; Bosch J; Pizcueta P. 2002. Concanavalin-A-induced liver injury is severely impaired in mice deficient in P-selectin. J Leukoc Biol 72(2):262-70. [PubMed: 12149416]  [MGI Ref ID J:124455]

Mayadas TN. 1995. Gene knockout on p-selectin: its biology and function. Trends Cardiovasc Med 5(4):149-157.  [MGI Ref ID J:28867]

Mayadas TN; Mendrick DL; Brady HR; Tang T; Papayianni A; Assmann KJ; Wagner DD; Hynes RO; Cotran RS. 1996. Acute passive anti-glomerular basement membrane nephritis in P-selectin-deficient mice. Kidney Int 49(5):1342-9. [PubMed: 8731099]  [MGI Ref ID J:113194]

McCafferty DM; Kanwar S; Granger DN; Kubes P. 2000. E/P-selectin-deficient mice: an optimal mutation for abrogating antigen but not tumor necrosis factor-alpha-induced immune responses Eur J Immunol 30(8):2362-71. [PubMed: 10940927]  [MGI Ref ID J:63951]

McCafferty DM; Smith CW; Granger DN; Kubes P. 1999. Intestinal inflammation in adhesion molecule-deficient mice: an assessment of P-selectin alone and in combination with ICAM-1 or E-selectin. J Leukoc Biol 66(1):67-74. [PubMed: 10410991]  [MGI Ref ID J:56590]

Mendez-Ferrer S; Lucas D; Battista M; Frenette PS. 2008. Haematopoietic stem cell release is regulated by circadian oscillations. Nature 452(7186):442-7. [PubMed: 18256599]  [MGI Ref ID J:134224]

Nacher M; Blazquez AB; Shao B; Matesanz A; Prophete C; Berin MC; Frenette PS; Hidalgo A. 2011. Physiological Contribution of CD44 as a Ligand for E-Selectin during Inflammatory T-Cell Recruitment. Am J Pathol 178(5):2437-46. [PubMed: 21457936]  [MGI Ref ID J:171380]

Papayannopoulou T; Priestley GV; Nakamoto B; Zafiropoulos V; Scott LM. 2001. Molecular pathways in bone marrow homing: dominant role of alpha(4)beta(1) over beta(2)-integrins and selectins. Blood 98(8):2403-11. [PubMed: 11588037]  [MGI Ref ID J:115624]

Patrick AL; Rullo J; Beaudin S; Liaw P; Fox-Robichaud AE. 2007. Hepatic leukocyte recruitment in response to time-limited expression of TNF-alpha and IL-1beta. Am J Physiol Gastrointest Liver Physiol 293(4):G663-72. [PubMed: 17656447]  [MGI Ref ID J:126664]

Planck SR; Han YB; Park JM; O'Rourke L; Gutierrez-Ramos JC; Rosenbaum JT. 1998. The effect of genetic deficiency of adhesion molecules on the course of endotoxin-induced uveitis. Curr Eye Res 17(9):941-6. [PubMed: 9746442]  [MGI Ref ID J:114206]

Robinson SD; Frenette PS; Rayburn H; Cummiskey M; Ullman-Cullere M; Wagner DD; Hynes RO. 1999. Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A 96(20):11452-7. [PubMed: 10500197]  [MGI Ref ID J:57973]

Scheiermann C; Kunisaki Y; Lucas D; Chow A; Jang JE; Zhang D; Hashimoto D; Merad M; Frenette PS. 2012. Adrenergic nerves govern circadian leukocyte recruitment to tissues. Immunity 37(2):290-301. [PubMed: 22863835]  [MGI Ref ID J:187383]

Singh I; Zibari GB; Brown MF; Granger DN; Eppihimer M; Zizzi H; Cruz L; Meyer K; Gonzales E; McDonald JC. 1999. Role of P-selectin expression in hepatic ischemia and reperfusion injury. Clin Transplant 13(1 Pt 2):76-82. [PubMed: 10081641]  [MGI Ref ID J:103151]

Sobolev O; Stern P; Lacy-Hulbert A; Hynes RO. 2009. Natural killer cells require selectins for suppression of subcutaneous tumors. Cancer Res 69(6):2531-9. [PubMed: 19258505]  [MGI Ref ID J:146885]

Stokol T; O'Donnell P; Xiao L; Knight S; Stavrakis G; Botto M; von Andrian UH; Mayadas TN. 2004. C1q governs deposition of circulating immune complexes and leukocyte Fcgamma receptors mediate subsequent neutrophil recruitment. J Exp Med 200(7):835-46. [PubMed: 15466618]  [MGI Ref ID J:93949]

Stubke K; Wicklein D; Herich L; Schumacher U; Nehmann N. 2012. Selectin-deficiency reduces the number of spontaneous metastases in a xenograft model of human breast cancer. Cancer Lett 321(1):89-99. [PubMed: 22366582]  [MGI Ref ID J:185633]

Subramaniam M; Frenette PS; Saffaripour S; Johnson RC; Hynes RO; Wagner DD. 1996. Defects in hemostasis in P-selectin-deficient mice. Blood 87(4):1238-42. [PubMed: 8608210]  [MGI Ref ID J:31431]

Subramaniam M; Saffaripour S; Watson SR; Mayadas TN; Hynes RO; Wagner DD. 1995. Reduced recruitment of inflammatory cells in a contact hypersensitivity response in P-selectin-deficient mice. J Exp Med 181(6):2277-82. [PubMed: 7539046]  [MGI Ref ID J:110854]

Sweeney EA; Priestley GV; Nakamoto B; Collins RG; Beaudet AL; Papayannopoulou T. 2000. Mobilization of stem/progenitor cells by sulfated polysaccharides does not require selectin presence. Proc Natl Acad Sci U S A 97(12):6544-9. [PubMed: 10841555]  [MGI Ref ID J:62722]

Tang T; Frenette PS; Hynes RO; Wagner DD; Mayadas TN. 1996. Cytokine-induced meningitis is dramatically attenuated in mice deficient in endothelial selectins. J Clin Invest 97(11):2485-90. [PubMed: 8647940]  [MGI Ref ID J:107411]

Taverna D; Moher H; Crowley D; Borsig L; Varki A; Hynes RO. 2004. Increased primary tumor growth in mice null for beta3- or beta3/beta5-integrins or selectins. Proc Natl Acad Sci U S A 101(3):763-8. [PubMed: 14718670]  [MGI Ref ID J:88109]

Tomita H; Iwata Y; Ogawa F; Komura K; Shimizu K; Yoshizaki A; Hara T; Muroi E; Yanaba K; Bae S; Takenaka M; Hasegawa M; Fujimoto M; Sato S. 2009. P-selectin glycoprotein ligand-1 contributes to wound healing predominantly as a p-selectin ligand and partly as an e-selectin ligand. J Invest Dermatol 129(8):2059-67. [PubMed: 19177138]  [MGI Ref ID J:152512]

Tu L; Poe JC; Kadono T; Venturi GM; Bullard DC; Tedder TF; Steeber DA. 2002. A functional role for circulating mouse L-selectin in regulating leukocyte/endothelial cell interactions in vivo. J Immunol 169(4):2034-43. [PubMed: 12165530]  [MGI Ref ID J:120206]

Wang L; Brown JR; Varki A; Esko JD. 2002. Heparin's anti-inflammatory effects require glucosamine 6-O-sulfation and are mediated by blockade of L- and P-selectins. J Clin Invest 110(1):127-36. [PubMed: 12093896]  [MGI Ref ID J:112426]

Wicklein D; Schmidt A; Labitzky V; Ullrich S; Valent P; Schumacher U. 2013. E- and p-selectins are essential for repopulation of chronic myelogenous and chronic eosinophilic leukemias in a scid mouse xenograft model. PLoS One 8(7):e70139. [PubMed: 23922938]  [MGI Ref ID J:204363]

Yago T; Fu J; McDaniel JM; Miner JJ; McEver RP; Xia L. 2010. Core 1-derived O-glycans are essential E-selectin ligands on neutrophils. Proc Natl Acad Sci U S A 107(20):9204-9. [PubMed: 20439727]  [MGI Ref ID J:160571]

Yamada S; Mayadas TN; Yuan F; Wagner DD; Hynes RO; Melder RJ; Jain RK. 1995. Rolling in P-selectin-deficient mice is reduced but not eliminated in the dorsal skin. Blood 86(9):3487-92. [PubMed: 7579454]  [MGI Ref ID J:29814]

Zaph C; Scott P. 2003. Th1 cell-mediated resistance to cutaneous infection with Leishmania major is independent of P- and E-selectins. J Immunol 171(9):4726-32. [PubMed: 14568948]  [MGI Ref ID J:107364]

Zhang S; Condac E; Qiu H; Jiang J; Gutierrez-Sanchez G; Bergmann C; Handel T; Wang L. 2012. Heparin-induced leukocytosis requires 6-O-sulfation and is caused by blockade of selectin- and CXCL12 protein-mediated leukocyte trafficking in mice. J Biol Chem 287(8):5542-53. [PubMed: 22194593]  [MGI Ref ID J:182444]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, homozygotes may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   001026 BALB/cByJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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