Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129S2-Selltm1Hyn/J    (Changed: 29-APR-09 ) Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse   Donating Investigator Richard Hynes,   Massachusetts Institute of Technology

Description
Mice homozygous for the Sell^tm2Hyn mutation are viable and fertile. They are characterized by hypoplastic lymph nodes because of failure of lymphocytes to roll on high endothelial venules in the nodes.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
A PGK-puro cassette was used to delete a 2.1 kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. This mutation was created in 129S2/SvPas-derived D3 embryonic stem (ES) cells. This line has been backcrossed to C57BL/6 for more than 10 generations.

Related Strains

Strains carrying   Sell^tm2Hyn allele

002917

B6;129S2-Selltm2Hyn/J

View Strains carrying   Sell^tm2Hyn     (1 strain)

Strains carrying other alleles of Sell

008439	B6.129S2-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
008441	B6.129S2-Selltm3Hyn Selptm1Hyn/J
003807	B6;129S-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
003806	B6;129S-Seletm2Hyn Selltm4Hyn/J
003805	B6;129S-Selltm3Hyn Selptm1Hyn/J
002917	B6;129S2-Selltm2Hyn/J
008440	C.129S2(B6)-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
008442	C.129S2(B6)-Selltm3Hyn Selptm1Hyn/J
003860	NOD.129P2(B6)-Selltm1Tft/1LtJ
004943	NOD.Cg-Selltm1Tft Itgb7tm1Cgn/LtJ

View Strains carrying other alleles of Sell     (10 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Sell^tm2Hyn/Sell^tm2Hyn

        involves: 129S2/SvPas * C57BL/6

• immune system phenotype
• *normal* immune system phenotype
  • mutants and controls show similar neutrophil accumulation in sinusoids and parenchyma at 7 hours after galactosamine/bacterial endotoxin treatment   (MGI Ref ID J:106550)
• • abnormal leukocyte tethering or rolling
    • there is a two-thirds decrease in the number of rolling leukocytes within the mesenteric venules of mice treated with TNF   (MGI Ref ID J:57973)
    • there is also slight decrease in the number of adhering leukocytes within these mesenteric venules   (MGI Ref ID J:57973)
    • mean velocity of leukocytes is decreased   (MGI Ref ID J:57973)
  • decreased monocyte cell number
    • monocyte numbers are decreased by more than half in the blood   (MGI Ref ID J:57973)
  • impaired neutrophil recruitment
    • neutrophil influx in thioglycollate-induced peritonitis is inhibited at 2 hours after injection   (MGI Ref ID J:57973)
• hematopoietic system phenotype
• decreased monocyte cell number
  • monocyte numbers are decreased by more than half in the blood   (MGI Ref ID J:57973)
• tumorigenesis
• decreased metastatic potential   (MGI Ref ID J:106671)
• decreased tumor incidence
  • poorer survival of tumor cells   (MGI Ref ID J:106671)
• liver/biliary system phenotype
• *normal* liver/biliary system phenotype
  • liver injury including necrosis are similar between treated mutants and controls at 7 hours after treatment   (MGI Ref ID J:106550)
• cellular phenotype
• abnormal leukocyte tethering or rolling
  • there is a two-thirds decrease in the number of rolling leukocytes within the mesenteric venules of mice treated with TNF   (MGI Ref ID J:57973)
  • there is also slight decrease in the number of adhering leukocytes within these mesenteric venules   (MGI Ref ID J:57973)
  • mean velocity of leukocytes is decreased   (MGI Ref ID J:57973)

Sell^tm2Hyn/Sell^tm2Hyn

        involves: 129S2/SvPas * BALB/c

• immune system phenotype
• abnormal leukocyte tethering or rolling
  • less rolling leukocytes are observed in the postcapillary venules of inflamed ears 12 hours after immunization compared to controls   (MGI Ref ID J:114317)
  • velocity of these rolling leukocytes is greater than controls 1 hour after immunization   (MGI Ref ID J:114317)
• cellular phenotype
• abnormal leukocyte tethering or rolling
  • less rolling leukocytes are observed in the postcapillary venules of inflamed ears 12 hours after immunization compared to controls   (MGI Ref ID J:114317)
  • velocity of these rolling leukocytes is greater than controls 1 hour after immunization   (MGI Ref ID J:114317)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Internal/Organ Research
Wound Healing
      delayed/impaired

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Selltm2Hyn
Allele Name		targeted mutation 2, Richard Hynes
Allele Type		Targeted (knock-out)
Common Name(s)		L-selectin-;
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Sell, selectin, lymphocyte
Chromosome		1
Gene Common Name(s)		A.11; AI528707; CD62L; L-selectin; LAM1; LECAM-1; LECAM1; LEU8; LNHR; LSEL; LYAM1; Lnhr; Ly-22; Ly-m22; Lyam-1; Lyam1; PLNHR; TQ1; expressed sequence AI528707; lymph node homing receptor; lymphocyte adhesion molecule 1; lymphocyte antigen 22; lymphocyte antigen m22;
Molecular Note		The three selectin genes, Sele, Sell, and Selp, are found within a 300 kb genomic region. ES cells heterozygote for the Selptm1Hyn and Seletm1Hyn null alleles in cis to each other were targeted a third time. A PGK-puro cassette was used to delete a 2.1 kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. Flow cytometric analysis showed an absence of the L-selectin protein but not the two other selectin proteins in leukocytes isolated from homozygotes. Thus, this targeted mutation occurred in trans with the other two null mutations and were subsequently bred apart. [MGI Ref ID J:57973]

Genotyping

Genotyping Information

Genotyping Protocols

Sell^tm1Hyn, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Robinson SD; Frenette PS; Rayburn H; Cummiskey M; Ullman-Cullere M; Wagner DD; Hynes RO. 1999. Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A 96(20):11452-7. [PubMed: 10500197]  [MGI Ref ID J:57973]

Additional References

Sell^tm2Hyn related

Belanger SD; St-Pierre Y. 2005. Role of selectins in the triggering, growth, and dissemination of T-lymphoma cells: implication of L-selectin in the growth of thymic lymphoma. Blood 105(12):4800-6. [PubMed: 15705798]  [MGI Ref ID J:107461]

Csencsits KL; Pascual DW. 2002. Absence of L-selectin delays mucosal B cell responses in nonintestinal effector tissues. J Immunol 169(10):5649-59. [PubMed: 12421944]  [MGI Ref ID J:80056]

Csencsits KL; Walters N; Pascual DW. 2001. Cutting edge: dichotomy of homing receptor dependence by mucosal effector B cells: alpha(E) versus L-selectin. J Immunol 167(5):2441-5. [PubMed: 11509580]  [MGI Ref ID J:93848]

Czarneski J; Berguer P; Bekinschtein P; Kim DC; Hakimpour P; Wagner N; Nepomnaschy I; Piazzon I; Ross SR. 2002. Neonatal infection with a milk-borne virus is independent of beta7 integrin- and L-selectin-expressing lymphocytes. Eur J Immunol 32(4):945-56. [PubMed: 11920560]  [MGI Ref ID J:76001]

Eriksson EE. 2008. No detectable endothelial- or leukocyte-derived L-selectin ligand activity on the endothelium in inflamed cremaster muscle venules. J Leukoc Biol 84(1):93-103. [PubMed: 18381812]  [MGI Ref ID J:137752]

Fukuyama S; Hiroi T; Yokota Y; Rennert PD; Yanagita M; Kinoshita N; Terawaki S; Shikina T; Yamamoto M; Kurono Y; Kiyono H. 2002. Initiation of NALT organogenesis is independent of the IL-7R, LTbetaR, and NIK signaling pathways but requires the Id2 gene and CD3(-)CD4(+)CD45(+) cells. Immunity 17(1):31-40. [PubMed: 12150889]  [MGI Ref ID J:78101]

Gonda A; Gal I; Szanto S; Sarraj B; Glant TT; Hunyadi J; Mikecz K. 2005. CD44, but not l-selectin, is critically involved in leucocyte migration into the skin in a murine model of allergic dermatitis. Exp Dermatol 14(9):700-8. [PubMed: 16098130]  [MGI Ref ID J:114317]

Laubli H; Stevenson JL; Varki A; Varki NM; Borsig L. 2006. L-selectin facilitation of metastasis involves temporal induction of Fut7-dependent ligands at sites of tumor cell arrest. Cancer Res 66(3):1536-42. [PubMed: 16452210]  [MGI Ref ID J:106671]

Lawson JA; Burns AR; Farhood A; Lynn Bajt M; Collins RG; Smith CW; Jaeschke H. 2000. Pathophysiologic importance of E- and L-selectin for neutrophil-induced liver injury during endotoxemia in mice. Hepatology 32(5):990-8. [PubMed: 11050049]  [MGI Ref ID J:106550]

Lejtenyi D; Osmond DG; Miller SC. 2003. Natural killer cells and B lymphocytes in L-selectin and Mac-1/LFA-1 knockout mice: marker-dependent, but not cell lineage-dependent changes in the spleen and bone marrow. Immunobiology 207(2):129-35. [PubMed: 12675270]  [MGI Ref ID J:102758]

Martin AP; Coronel EC; Sano G; Chen SC; Vassileva G; Canasto-Chibuque C; Sedgwick JD; Frenette PS; Lipp M; Furtado GC; Lira SA. 2004. A novel model for lymphocytic infiltration of the thyroid gland generated by transgenic expression of the CC chemokine CCL21. J Immunol 173(8):4791-8. [PubMed: 15470018]  [MGI Ref ID J:93715]

Pascual DW; Riccardi C; Csencsits-Smith K. 2008. Distal IgA immunity can be sustained by alphaEbeta7+ B cells in L-selectin-/- mice following oral immunization. Mucosal Immunol 1(1):68-77. [PubMed: 19079162]  [MGI Ref ID J:161882]

Sobolev O; Stern P; Lacy-Hulbert A; Hynes RO. 2009. Natural killer cells require selectins for suppression of subcutaneous tumors. Cancer Res 69(6):2531-9. [PubMed: 19258505]  [MGI Ref ID J:146885]

Sweeney EA; Priestley GV; Nakamoto B; Collins RG; Beaudet AL; Papayannopoulou T. 2000. Mobilization of stem/progenitor cells by sulfated polysaccharides does not require selectin presence. Proc Natl Acad Sci U S A 97(12):6544-9. [PubMed: 10841555]  [MGI Ref ID J:62722]

Szanto S; Gal I; Gonda A; Glant TT; Mikecz K. 2004. Expression of L-selectin, but not CD44, is required for early neutrophil extravasation in antigen-induced arthritis. J Immunol 172(11):6723-34. [PubMed: 15153489]  [MGI Ref ID J:90526]

Taverna D; Moher H; Crowley D; Borsig L; Varki A; Hynes RO. 2004. Increased primary tumor growth in mice null for beta3- or beta3/beta5-integrins or selectins. Proc Natl Acad Sci U S A 101(3):763-8. [PubMed: 14718670]  [MGI Ref ID J:88109]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Colony Maintenance

Breeding & Husbandry	When maintained as a live colony, homozygotes may be bred.

Purchasing information

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• This strain is included in the Induced Mutant Resource Colony collection.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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