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| Mice harboring the CAG-luc-eGFP L2G85 transgene exhibit widespread expression of firefly luciferase and enhanced green fluorescence protein directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). Transgene expression is reported in all tissues and organs, except mature erythrocytes. These CAG-luc-eGFP L2G85 transgenic mice may be useful in studies of transplantation, noninvasive lineage mapping, fluorescent and in vivo biolouminescence imaging and technology development. | |||||||||||||||||||
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Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 27-OCT-09 Species laboratory mouse Generation N21+ (10-JUL-09) Donating Investigator Christopher Contag, Stanford University Description
Mice homozygous for the CAG-luc-eGFP L2G85 transgene are viable and fertile, with widespread expression of firefly luciferase and enhanced green fluorescence protein directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). Transgene expression is reported in all tissues and organs, except mature erythrocytes. Homozygotes have no reported gross physical or behavioral abnormalities. Following luciferin injection, luciferase expression is generally greater in males than females. These CAG-luc-eGFP L2G85 transgene may be useful in studies of transplantation, noninvasive lineage mapping, fluorescent and in vivo biolouminescence imaging and technology development.Development
The CAG-luc-eGFP transgenic construct was designed by the laboratory of Dr. Christopher H. Contag (Stanford University School of Medicine). Specifically, the transgene contained the human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector) upstream of a modified firefly luciferase gene open reading frame, 54 bp of the foot and mouth disease virus (FMDV) 2A sequence (ribsome slippage site), 24 bp polylinker, and enhanced Green Fluorescent Protein (eGFP) open reading frame. This transgene was injected into fertilized FVB mouse eggs. Founder line L2G85 animals were bred to wildtype FVB mice. These FVB-L2G85 (or FVB.luc) transgenic were then backcrossed to FVB for 20 generations prior to arrival at The Jackson Laboratory. Upon arrival, CAG-luc-eGFP transgenic mice were bred with FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cancer Research
xenograft/transplant host
Developmental Biology Research
transplantation marker for embryonic and adult tissue
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: cell marker for bone marrow transplantation
Tissue/Cell Markers: multiple
Reproductive Biology Research
spermatogonial transplantation marker
transplantation marker for embryonic and adult tissue
Toxicology Research
B and T cell deficiency, xenograft transplant host
xenograft/transplant host
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-luc,-GFP)L2G85Chco | ||
|---|---|---|---|
| Allele Name | transgene insertion L2G85, Christopher H Contag | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | L2G85; luc+; | ||
| Mutation Made By | Ryan McBride Spitler, Stanford University | ||
| Strain of Origin | FVB | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | CMV, cytomegalovirus, human | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | A transgenic construct containing a modified firefly luciferase gene open reading frame, 54bp of the foot and mouth disease virus (FMDV) 2A sequence (ribsome slippage site) and eGFP sequence under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer (CAG), was injected into fertilized FVB mouse eggs. Founder line L2G85 animals were bred to wildtype FVB mice. [MGI Ref ID J:148234] | ||
| Gene Symbol and Name | Tg(CAG-luc,-GFP)L2G85Chco, transgene insertion L2G85, Christopher H Contag | ||
| Chromosome | UN | ||
| Gene Common Name(s) | L2G85; luc+; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins -- Generic GFP, QPCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Cao YA; Wagers AJ; Beilhack A; Dusich J; Bachmann MH; Negrin RS; Weissman IL; Contag CH. 2004. Shifting foci of hematopoiesis during reconstitution from single stem cells. Proc Natl Acad Sci U S A 101(1):221-6. [PubMed: 14688412] [MGI Ref ID J:148234]
Sheikh AY; Lin SA; Cao F; Cao Y; van der Bogt KE; Chu P; Chang CP; Contag CH; Robbins RC; Wu JC. 2007. Molecular imaging of bone marrow mononuclear cell homing and engraftment in ischemic myocardium. Stem Cells 25(10):2677-84. [PubMed: 17628019] [MGI Ref ID J:148235]
Additional References
Tg(CAG-luc,-GFP)L2G85Chco relatedCreusot RJ; Yaghoubi SS; Chang P; Chia J; Contag CH; Gambhir SS; Fathman CG. 2009. Lymphoid-tissue-specific homing of bone-marrow-derived dendritic cells. Blood 113(26):6638-47. [PubMed: 19363220] [MGI Ref ID J:152743]
Fernandez I; Zeiser R; Karsunky H; Kambham N; Beilhack A; Soderstrom K; Negrin RS; Engleman E. 2007. CD101 surface expression discriminates potency among murine FoxP3+ regulatory T cells. J Immunol 179(5):2808-14. [PubMed: 17709494] [MGI Ref ID J:151833]
Zeiser R; Youssef S; Baker J; Kambham N; Steinman L; Negrin RS. 2007. Preemptive HMG-CoA reductase inhibition provides graft-versus-host disease protection by Th-2 polarization while sparing graft-versus-leukemia activity. Blood 110(13):4588-98. [PubMed: 17827390] [MGI Ref ID J:149097]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) 27-OCT-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 07-DEC-09
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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