Description

Strain Information

Former Names STOCK Mir7-92tm1.1Tyj/J    (Changed: 21-JUL-11 ) STOCK Mir17-92tm1.1Tyj/J    (Changed: 21-JUL-11 ) STOCK Mirn17-92tm1.1Tyj/J    (Changed: 27-JAN-09 ) STOCK Mirn17-92tm1Tyj/J    (Changed: 07-JUL-08 ) STOCK Mirn17-Mirn92-1tm1Tyj/J    (Changed: 26-JUN-08 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote         (Female x Male)   01-AUG-09 Species laboratory mouse Generation F?+N1F8 (22-NOV-11) Generation Definitions   Donating Investigator Dr. Tyler Jacks,   Massachusetts Institute of Technology

Description
The miR-17~92 (Mir17, Mir18, Mir19a, Mir20a, Mir19b-1, Mir92-1) cluster, overexpressed in human cancers, is flanked by loxP sites in this targeted mutation strain. Mice homozygous for the floxed miR17~92 allele (miR17~92^fl/fl) are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

Development
LoxP sites were introduced 5' of the Mirn17 gene and 3' of the Mirn92-1 gene. The floxed region was followed by an Frt-flanked neomycin cassette. The targeting vector was introduced to V6.5 (C57BL/6 x 129S4/SvJae)F1-derived embryonic stem (ES) cells. The neomycin cassette was removed through Flp-mediated excision via a C57BL/6 background strain. This strain was maintained on a mixed C57BL/6 and 129S4 background by the donating laboratory.

Control Information

	Control
	None Available
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Mirc1

008459

STOCK Mirc1tm1.2Tyj/J

View Strains carrying other alleles of Mirc1     (1 strain)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Mirc1^tm1.1Tyj/Mirc1^tm1.1Tyj

        involves: 129S4/SvJae * C57BL/6 * SJL

• normal phenotype
• no abnormal phenotype detected
  • viable with no obvious abnormalities   (MGI Ref ID J:134861)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cancer Research
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Mirc1tm1.1Tyj
Allele Name		targeted mutation 1.1, Tyler Jacks
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		Mir17-92tm1.1Tyj; miR17~92fl;
Mutation Made By		Dr. Tyler Jacks,   Massachusetts Institute of Technology
Strain of Origin		(C57BL/6 x 129S4/SvJae)F1
ES Cell Line Name		v6.5
ES Cell Line Strain		(C57BL/6 x 129S4/SvJae)F1
Gene Symbol and Name		Mirc1, microRNA cluster 1, including Mir17 through Mir92-1
Chromosome		14
Gene Common Name(s)		Mir17-92; Mirn17-92; miR-17-92; miR-17~92; microRNA cluster 17-92;
Molecular Note		The Frt-flanked neomycin cassette in Mir17-92tm1Tyj was removed through Flp-mediated excision. [MGI Ref ID J:134861]

Genotyping

Genotyping Information

Genotyping Protocols

Mirn17-92^tm1.1Tyj, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ventura A; Young AG; Winslow MM; Lintault L; Meissner A; Erkeland SJ; Newman J; Bronson RT; Crowley D; Stone JR; Jaenisch R; Sharp PA; Jacks T. 2008. Targeted deletion reveals essential and overlapping functions of the miR-17 through 92 family of miRNA clusters. Cell 132(5):875-86. [PubMed: 18329372]  [MGI Ref ID J:134861]

Additional References

Mirc1^tm1.1Tyj related

Budde H; Schmitt S; Fitzner D; Opitz L; Salinas-Riester G; Simons M. 2010. Control of oligodendroglial cell number by the miR-17-92 cluster. Development 137(13):2127-32. [PubMed: 20504959]  [MGI Ref ID J:161956]

Chong MM; Zhang G; Cheloufi S; Neubert TA; Hannon GJ; Littman DR. 2010. Canonical and alternate functions of the microRNA biogenesis machinery. Genes Dev 24(17):1951-60. [PubMed: 20713509]  [MGI Ref ID J:163823]

Conkrite K; Sundby M; Mukai S; Thomson JM; Mu D; Hammond SM; Macpherson D. 2011. miR-17~92 cooperates with RB pathway mutations to promote retinoblastoma. Genes Dev 25(16):1734-45. [PubMed: 21816922]  [MGI Ref ID J:174680]

Feuermann Y; Robinson GW; Zhu BM; Kang K; Raviv N; Yamaji D; Hennighausen L. 2012. The miR-17/92 cluster is targeted by STAT5 but dispensable for mammary development. Genesis 50(9):665-71. [PubMed: 22389215]  [MGI Ref ID J:187861]

Jiang S; Li C; Olive V; Lykken E; Feng F; Sevilla J; Wan Y; He L; Li QJ. 2011. Molecular dissection of the miR-17-92 cluster's critical dual roles in promoting Th1 responses and preventing inducible Treg differentiation. Blood 118(20):5487-97. [PubMed: 21972292]  [MGI Ref ID J:178854]

Mu P; Han YC; Betel D; Yao E; Squatrito M; Ogrodowski P; de Stanchina E; D'Andrea A; Sander C; Ventura A. 2009. Genetic dissection of the miR-17~92 cluster of microRNAs in Myc-induced B-cell lymphomas. Genes Dev 23(24):2806-11. [PubMed: 20008931]  [MGI Ref ID J:155803]

Nittner D; Lambertz I; Clermont F; Mestdagh P; Kohler C; Nielsen SJ; Jochemsen A; Speleman F; Vandesompele J; Dyer MA; Schramm A; Schulte JH; Marine JC. 2012. Synthetic lethality between Rb, p53 and Dicer or miR-17-92 in retinal progenitors suppresses retinoblastoma formation. Nat Cell Biol 14(9):958-65. [PubMed: 22864477]  [MGI Ref ID J:193515]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & Husbandry	When maintained as a live colony, homozygotes or heterozygotes may be bred.
Mating System	Homozygote x Homozygote         (Female x Male)   01-AUG-09
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	None Available
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.