Strain Name:

STOCK Mirc1tm1.1Tyj/J

Stock Number:

008458

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
The miR-17~92 (microRNA17-92) cluster is flanked by loxP sites in this targeted mutation strain. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

Description

Strain Information

Former Names STOCK Mir7-92tm1.1Tyj/J    (Changed: 21-JUL-11 )
STOCK Mir17-92tm1.1Tyj/J    (Changed: 21-JUL-11 )
STOCK Mirn17-92tm1.1Tyj/J    (Changed: 27-JAN-09 )
STOCK Mirn17-92tm1Tyj/J    (Changed: 07-JUL-08 )
STOCK Mirn17-Mirn92-1tm1Tyj/J    (Changed: 26-JUN-08 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-AUG-09
Specieslaboratory mouse
GenerationF?+N1F12 (11-DEC-13)
Generation Definitions
 
Donating InvestigatorDr. Tyler Jacks,   Massachusetts Institute of Technology

Description
The miR-17~92 (Mir17, Mir18, Mir19a, Mir20a, Mir19b-1, Mir92-1) cluster, overexpressed in human cancers, is flanked by loxP sites in this targeted mutation strain. Mice homozygous for the floxed miR17~92 allele (miR17~92fl/fl) are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

Development
LoxP sites were introduced 5' of the Mirn17 gene and 3' of the Mirn92-1 gene. The floxed region was followed by an Frt-flanked neomycin cassette. The targeting vector was introduced to V6.5 (C57BL/6 x 129S4/SvJae)F1-derived embryonic stem (ES) cells. The neomycin cassette was removed through Flp-mediated excision via a C57BL/6 background strain. This strain was maintained on a mixed C57BL/6 and 129S4 background by the donating laboratory.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Mirc1
008459   STOCK Mirc1tm1.2Tyj/J
View Strains carrying other alleles of Mirc1     (1 strain)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Mirc1tm1.1Tyj/Mirc1tm1.1Tyj

        involves: 129S4/SvJae * C57BL/6 * SJL
  • normal phenotype
  • no abnormal phenotype detected
    • viable with no obvious abnormalities   (MGI Ref ID J:134861)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cancer Research
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Mirc1tm1.1Tyj
Allele Name targeted mutation 1.1, Tyler Jacks
Allele Type Targeted (Floxed/Frt)
Common Name(s) Mir17-92tm1.1Tyj; miR17~92fl;
Mutation Made ByDr. Tyler Jacks,   Massachusetts Institute of Technology
Strain of Origin(C57BL/6 x 129S4/SvJae)F1
ES Cell Line Namev6.5
ES Cell Line Strain(C57BL/6 x 129S4/SvJae)F1
Gene Symbol and Name Mirc1, microRNA cluster 1, including Mir17 through Mir92-1
Chromosome 14
Gene Common Name(s) Mir17-92; Mirn17-92; miR-17-92; miR-17~92; microRNA cluster 17-92;
Molecular Note The Frt-flanked neomycin cassette in Mir17-92tm1Tyj was removed through Flp-mediated excision. [MGI Ref ID J:134861]

Genotyping

Genotyping Information

Genotyping Protocols

Mirn17-92tm1.1Tyj, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ventura A; Young AG; Winslow MM; Lintault L; Meissner A; Erkeland SJ; Newman J; Bronson RT; Crowley D; Stone JR; Jaenisch R; Sharp PA; Jacks T. 2008. Targeted deletion reveals essential and overlapping functions of the miR-17 through 92 family of miRNA clusters. Cell 132(5):875-86. [PubMed: 18329372]  [MGI Ref ID J:134861]

Additional References

Mirc1tm1.1Tyj related

Baumjohann D; Kageyama R; Clingan JM; Morar MM; Patel S; de Kouchkovsky D; Bannard O; Bluestone JA; Matloubian M; Ansel KM; Jeker LT. 2013. The microRNA cluster miR-17 approximately 92 promotes TFH cell differentiation and represses subset-inappropriate gene expression. Nat Immunol 14(8):840-8. [PubMed: 23812098]  [MGI Ref ID J:205722]

Budde H; Schmitt S; Fitzner D; Opitz L; Salinas-Riester G; Simons M. 2010. Control of oligodendroglial cell number by the miR-17-92 cluster. Development 137(13):2127-32. [PubMed: 20504959]  [MGI Ref ID J:161956]

Chong MM; Zhang G; Cheloufi S; Neubert TA; Hannon GJ; Littman DR. 2010. Canonical and alternate functions of the microRNA biogenesis machinery. Genes Dev 24(17):1951-60. [PubMed: 20713509]  [MGI Ref ID J:163823]

Conkrite K; Sundby M; Mukai S; Thomson JM; Mu D; Hammond SM; Macpherson D. 2011. miR-17~92 cooperates with RB pathway mutations to promote retinoblastoma. Genes Dev 25(16):1734-45. [PubMed: 21816922]  [MGI Ref ID J:174680]

Fan P; Chen Z; Tian P; Liu W; Jiao Y; Xue Y; Bhattacharya A; Wu J; Lu M; Guo Y; Cui Y; Gu W; Gu W; Yue J. 2013. miRNA biogenesis enzyme Drosha is required for vascular smooth muscle cell survival. PLoS One 8(4):e60888. [PubMed: 23637774]  [MGI Ref ID J:200563]

Feuermann Y; Robinson GW; Zhu BM; Kang K; Raviv N; Yamaji D; Hennighausen L. 2012. The miR-17/92 cluster is targeted by STAT5 but dispensable for mammary development. Genesis 50(9):665-71. [PubMed: 22389215]  [MGI Ref ID J:187861]

Jiang S; Li C; Olive V; Lykken E; Feng F; Sevilla J; Wan Y; He L; Li QJ. 2011. Molecular dissection of the miR-17-92 cluster's critical dual roles in promoting Th1 responses and preventing inducible Treg differentiation. Blood 118(20):5487-97. [PubMed: 21972292]  [MGI Ref ID J:178854]

Jin HY; Oda H; Lai M; Skalsky RL; Bethel K; Shepherd J; Kang SG; Liu WH; Sabouri-Ghomi M; Cullen BR; Rajewsky K; Xiao C. 2013. MicroRNA-17 approximately 92 plays a causative role in lymphomagenesis by coordinating multiple oncogenic pathways. EMBO J 32(17):2377-91. [PubMed: 23921550]  [MGI Ref ID J:200069]

Kang SG; Liu WH; Lu P; Jin HY; Lim HW; Shepherd J; Fremgen D; Verdin E; Oldstone MB; Qi H; Teijaro JR; Xiao C. 2013. MicroRNAs of the miR-17 approximately 92 family are critical regulators of T(FH) differentiation. Nat Immunol 14(8):849-57. [PubMed: 23812097]  [MGI Ref ID J:201798]

Khan AA; Penny LA; Yuzefpolskiy Y; Sarkar S; Kalia V. 2013. MicroRNA-17~92 regulates effector and memory CD8 T-cell fates by modulating proliferation in response to infections. Blood 121(22):4473-83. [PubMed: 23596046]  [MGI Ref ID J:198459]

Mu P; Han YC; Betel D; Yao E; Squatrito M; Ogrodowski P; de Stanchina E; D'Andrea A; Sander C; Ventura A. 2009. Genetic dissection of the miR-17~92 cluster of microRNAs in Myc-induced B-cell lymphomas. Genes Dev 23(24):2806-11. [PubMed: 20008931]  [MGI Ref ID J:155803]

Murphy BL; Obad S; Bihannic L; Ayrault O; Zindy F; Kauppinen S; Roussel MF. 2013. Silencing of the miR-17~92 cluster family inhibits medulloblastoma progression. Cancer Res 73(23):7068-78. [PubMed: 24145352]  [MGI Ref ID J:206525]

Nittner D; Lambertz I; Clermont F; Mestdagh P; Kohler C; Nielsen SJ; Jochemsen A; Speleman F; Vandesompele J; Dyer MA; Schramm A; Schulte JH; Marine JC. 2012. Synthetic lethality between Rb, p53 and Dicer or miR-17-92 in retinal progenitors suppresses retinoblastoma formation. Nat Cell Biol 14(9):958-65. [PubMed: 22864477]  [MGI Ref ID J:193515]

Patel V; Williams D; Hajarnis S; Hunter R; Pontoglio M; Somlo S; Igarashi P. 2013. miR-17~92 miRNA cluster promotes kidney cyst growth in polycystic kidney disease. Proc Natl Acad Sci U S A 110(26):10765-70. [PubMed: 23759744]  [MGI Ref ID J:197969]

de Kouchkovsky D; Esensten JH; Rosenthal WL; Morar MM; Bluestone JA; Jeker LT. 2013. microRNA-17-92 regulates IL-10 production by regulatory T cells and control of experimental autoimmune encephalomyelitis. J Immunol 191(4):1594-605. [PubMed: 23858035]  [MGI Ref ID J:205831]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, homozygotes or heterozygotes may be bred.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-AUG-09
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Mirc1tm1.1Tyj  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Mirc1tm1.1Tyj x Homozygous for Mirc1tm1.1Tyj  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Mirc1tm1.1Tyj  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Mirc1tm1.1Tyj x Homozygous for Mirc1tm1.1Tyj  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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