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| A conditional Cre-ERT2 (Cre recombinase - estrogen receptor T2) cassette was introduced to the gene. The ERT2 moiety retains the Cre recombinase in the cytoplasm until tamoxifen administration releases this inhibition, thus permitting the recombination of genomic loxP sites. Efficient tamoxifen-induced Cre-mediated recombination throughout the body has been demonstrated through crosses with a Cre-responsive beta galactosidase reporter strain. This strain enables temporal control of floxed gene expression in vivo and is reportedly more sensitive to tamoxifen than Stock No. 004847. Homozygotes are viable and fertile. | |||||||||||||||||||
- Description
- Phenotype
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Description
Strain Information
Former Names B6.129-Gt(ROSA)26Sortm1(cre/ESR1)Tyj/J (Changed: 17-MAR-10 ) B6;129-Gt(ROSA)26Sortm1(cre/ESR1)Tyj/J (Changed: 12-JUN-08 ) Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 16-DEC-09 Species laboratory mouse Generation N9+F6 (20-DEC-11)
Generation DefinitionsDonating Investigator Tyler Jacks, Massachusetts Institute of Technology Description
A conditional Cre-ERT2 (Cre recombinase - estrogen receptor T2) cassette was introduced to the gene. The ERT2 moiety retains the Cre recombinase in the cytoplasm until tamoxifen administration releases this inhibition, thus permitting the recombination of genomic loxP sites. Efficient tamoxifen-induced Cre-mediated recombination throughout the body has been demonstrated through crosses with a Cre-responsive beta galactosidase reporter strain. This strain enables temporal control of floxed gene expression in vivo and is reportedly more sensitive to tamoxifen than Stock No. 004847. Homozygotes are viable and fertile.Development
A conditional Cre-ERT2 (estrogen receptor T2) cassette was inserted to intron 1 of the gene. The mutation was created in 129S4/SvJae-derived J1 embryonic stem (ES) cells and backcrossed 8 times to C57BL/6 by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (104 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cancer Research
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Inducible
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1(cre/ERT2)Tyj | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Tyler Jacks | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Cre-ERT2; Gt(ROSA)26Sortm1(cre/ESR1)Tyj; R26-Cre-ERT2; | ||
| Mutation Made By | Tyler Jacks, Massachusetts Institute of Technology | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Driver Note | Gt(ROSA)26Sor | ||
| Inducible Note | induced by tamoxifen | ||
| Molecular Note | A cassette containing Cre-ERT2 was inserted into the locus. [MGI Ref ID J:118233] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(cre/ERT2)Tyj, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Ventura A; Kirsch DG; McLaughlin ME; Tuveson DA; Grimm J; Lintault L; Newman J; Reczek EE; Weissleder R; Jacks T. 2007. Restoration of p53 function leads to tumour regression in vivo. Nature 445(7128):661-5. [PubMed: 17251932] [MGI Ref ID J:118233]
Additional References
Gt(ROSA)26Sortm1(cre/ERT2)Tyj relatedBrady CA; Jiang D; Mello SS; Johnson TM; Jarvis LA; Kozak MM; Kenzelmann Broz D; Basak S; Park EJ; McLaughlin ME; Karnezis AN; Attardi LD. 2011. Distinct p53 transcriptional programs dictate acute DNA-damage responses and tumor suppression. Cell 145(4):571-83. [PubMed: 21565614] [MGI Ref ID J:173395]
Chen H; Gu X; Liu Y; Wang J; Wirt SE; Bottino R; Schorle H; Sage J; Kim SK. 2011. PDGF signalling controls age-dependent proliferation in pancreatic beta-cells. Nature 478(7369):349-55. [PubMed: 21993628] [MGI Ref ID J:177411]
Haigis KM; Kendall KR; Wang Y; Cheung A; Haigis MC; Glickman JN; Niwa-Kawakita M; Sweet-Cordero A; Sebolt-Leopold J; Shannon KM; Settleman J; Giovannini M; Jacks T. 2008. Differential effects of oncogenic K-Ras and N-Ras on proliferation, differentiation and tumor progression in the colon. Nat Genet 40(5):600-8. [PubMed: 18372904] [MGI Ref ID J:132357]
Jiang D; Brady CA; Johnson TM; Lee EY; Park EJ; Scott MP; Attardi LD. 2011. Full p53 transcriptional activation potential is dispensable for tumor suppression in diverse lineages. Proc Natl Acad Sci U S A 108(41):17123-8. [PubMed: 21969549] [MGI Ref ID J:177455]
Kaiser WJ; Upton JW; Long AB; Livingston-Rosanoff D; Daley-Bauer LP; Hakem R; Caspary T; Mocarski ES. 2011. RIP3 mediates the embryonic lethality of caspase-8-deficient mice. Nature 471(7338):368-72. [PubMed: 21368762] [MGI Ref ID J:170815]
Meylan E; Dooley AL; Feldser DM; Shen L; Turk E; Ouyang C; Jacks T. 2009. Requirement for NF-kappaB signalling in a mouse model of lung adenocarcinoma. Nature 462(7269):104-7. [PubMed: 19847165] [MGI Ref ID J:154041]
Mu P; Han YC; Betel D; Yao E; Squatrito M; Ogrodowski P; de Stanchina E; D'Andrea A; Sander C; Ventura A. 2009. Genetic dissection of the miR-17~92 cluster of microRNAs in Myc-induced B-cell lymphomas. Genes Dev 23(24):2806-11. [PubMed: 20008931] [MGI Ref ID J:155803]
Nguyen AT; Taranova O; He J; Zhang Y. 2011. DOT1L, the H3K79 methyltransferase, is required for MLL-AF9-mediated leukemogenesis. Blood 117(25):6912-22. [PubMed: 21521783] [MGI Ref ID J:174817]
Pajcini KV; Corbel SY; Sage J; Pomerantz JH; Blau HM. 2010. Transient inactivation of Rb and ARF yields regenerative cells from postmitotic mammalian muscle. Cell Stem Cell 7(2):198-213. [PubMed: 20682446] [MGI Ref ID J:163316]
Viatour P; Somervaille TC; Venkatasubrahmanyam S; Kogan S; McLaughlin ME; Weissman IL; Butte AJ; Passegue E; Sage J. 2008. Hematopoietic stem cell quiescence is maintained by compound contributions of the retinoblastoma gene family. Cell Stem Cell 3(4):416-28. [PubMed: 18940733] [MGI Ref ID J:149791]
Wang J; Kumar RM; Biggs VJ; Lee H; Chen Y; Kagey MH; Young RA; Abate-Shen C. 2011. The Msx1 Homeoprotein Recruits Polycomb to the Nuclear Periphery during Development. Dev Cell 21(3):575-88. [PubMed: 21852201] [MGI Ref ID J:178355]
Yao R; Natsume Y; Saiki Y; Shioya H; Takeuchi K; Yamori T; Toki H; Aoki I; Saga T; Noda T. 2012. Disruption of Tacc3 function leads to in vivo tumor regression. Oncogene 31(2):135-48. [PubMed: 21685933] [MGI Ref ID J:179414]
Young NP; Crowley D; Jacks T. 2011. Uncoupling Cancer Mutations Reveals Critical Timing of p53 Loss in Sarcomagenesis. Cancer Res 71(11):4040-7. [PubMed: 21512139] [MGI Ref ID J:172206]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, homozygotes or heterozygotes may be bred. Mating System Homozygote x Homozygote (Female x Male) 16-DEC-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $172.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(cre/ESR1)Tyj
Pairs /Price (US dollars $) Pair Genotype $344.00 Homozygous for Gt(ROSA)26Sortm1(cre/ESR1)Tyj x Homozygous for Gt(ROSA)26Sortm1(cre/ESR1)Tyj Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $223.60 Female or Male Homozygous for Gt(ROSA)26Sortm1(cre/ESR1)Tyj
Pairs /Price (US dollars $) Pair Genotype $447.20 Homozygous for Gt(ROSA)26Sortm1(cre/ESR1)Tyj x Homozygous for Gt(ROSA)26Sortm1(cre/ESR1)Tyj Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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