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| A conditional Cre-ERT2 (Cre recombinase - estrogen receptor T2) cassette was introduced to the gene. The ERT2 moiety retains the Cre recombinase in the cytoplasm until tamoxifen administration releases this inhibition, thus permitting the recombination of genomic loxP sites. Efficient tamoxifen-induced Cre-mediated recombination throughout the body has been demonstrated through crosses with a Cre-responsive beta galactosidase reporter strain. This strain enables temporal control of floxed gene expression in vivo and is reportedly more sensitive to tamoxifen than Stock No. 004847. Homozygotes are viable and fertile. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6;129-Gt(ROSA)26Sortm1(cre/ESR1)Tyj/J (Changed: 12-JUN-08 ) Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Heterozygote (Female x Male) 30-APR-09 Species laboratory mouse Generation N9+ (03-FEB-09) Donating Investigator Tyler Jacks, Massachusetts Institute of Technology Description
A conditional Cre-ERT2 (Cre recombinase - estrogen receptor T2) cassette was introduced to the gene. The ERT2 moiety retains the Cre recombinase in the cytoplasm until tamoxifen administration releases this inhibition, thus permitting the recombination of genomic loxP sites. Efficient tamoxifen-induced Cre-mediated recombination throughout the body has been demonstrated through crosses with a Cre-responsive beta galactosidase reporter strain. This strain enables temporal control of floxed gene expression in vivo and is reportedly more sensitive to tamoxifen than Stock No. 004847. Homozygotes are viable and fertile.Development
A conditional Cre-ERT2 (estrogen receptor T2) cassette was inserted to intron 1 of the gene. The mutation was created in 129S4/SvJae-derived J1 embryonic stem (ES) cells and backcrossed 8 times to C57BL/6 by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (62 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Cancer Research
Cre-lox System
Cre Recombinase Expression: Inducible
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm1(cre/ESR1)Tyj | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Tyler Jacks | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | R26-Cre-ERT2; | ||
| Mutation Made By | Tyler Jacks, Massachusetts Institute of Technology | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Driver Note | Gt(ROSA)26Sor | ||
| Molecular Note | A cassette containing Cre-ERT2 was inserted into the locus. [MGI Ref ID J:118233] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(cre/ESR1)Tyj, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Ventura A; Kirsch DG; McLaughlin ME; Tuveson DA; Grimm J; Lintault L; Newman J; Reczek EE; Weissleder R; Jacks T. 2007. Restoration of p53 function leads to tumour regression in vivo. Nature 445(7128):661-5. [PubMed: 17251932] [MGI Ref ID J:118233]
Additional References
Gt(ROSA)26Sortm1(cre/ESR1)Tyj relatedHaigis KM; Kendall KR; Wang Y; Cheung A; Haigis MC; Glickman JN; Niwa-Kawakita M; Sweet-Cordero A; Sebolt-Leopold J; Shannon KM; Settleman J; Giovannini M; Jacks T. 2008. Differential effects of oncogenic K-Ras and N-Ras on proliferation, differentiation and tumor progression in the colon. Nat Genet 40(5):600-8. [PubMed: 18372904] [MGI Ref ID J:132357]
Viatour P; Somervaille TC; Venkatasubrahmanyam S; Kogan S; McLaughlin ME; Weissman IL; Butte AJ; Passegue E; Sage J. 2008. Hematopoietic stem cell quiescence is maintained by compound contributions of the retinoblastoma gene family. Cell Stem Cell 3(4):416-28. [PubMed: 18940733] [MGI Ref ID J:149791]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, homozygotes or heterozygotes may be bred. Mating System Heterozygote x Heterozygote (Female x Male) 30-APR-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Heterozygous for Gt(ROSA)26Sortm1(cre/ESR1)Tyj $300.70 Female or Male Homozygous for Gt(ROSA)26Sortm1(cre/ESR1)Tyj
Pairs /Price (US dollars $) Pair Genotype $487.00 Heterozygous for Gt(ROSA)26Sortm1(cre/ESR1)Tyj x Heterozygous for Gt(ROSA)26Sortm1(cre/ESR1)Tyj
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Heterozygous for Gt(ROSA)26Sortm1(cre/ESR1)Tyj $391.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(cre/ESR1)Tyj
Pairs /Price (US dollars $) Pair Genotype $633.10 Heterozygous for Gt(ROSA)26Sortm1(cre/ESR1)Tyj x Heterozygous for Gt(ROSA)26Sortm1(cre/ESR1)Tyj
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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