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| A conditional Cre-ERT2 (Cre recombinase - estrogen receptor T2) cassette was introduced to the gene. The ERT2 moiety retains the Cre recombinase in the cytoplasm until tamoxifen administration releases this inhibition, thus permitting the recombination of genomic loxP sites. Efficient tamoxifen-induced Cre-mediated recombination throughout the body has been demonstrated through crosses with a Cre-responsive beta galactosidase reporter strain. This strain enables temporal control of floxed gene expression in vivo and is reportedly more sensitive to tamoxifen than Stock No. 004847. Homozygotes are viable and fertile. | |||||||||||||||||||
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Description
Strain Information
Former Names B6.129-Gt(ROSA)26Sortm1(cre/ESR1)Tyj/J (Changed: 17-MAR-10 ) B6;129-Gt(ROSA)26Sortm1(cre/ESR1)Tyj/J (Changed: 12-JUN-08 ) Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 16-DEC-09 Species laboratory mouse Generation N9+F7 (18-JUN-12)
Generation DefinitionsDonating Investigator Dr. Tyler Jacks, Massachusetts Institute of Technology Description
A conditional Cre-ERT2 (Cre recombinase - estrogen receptor T2) cassette was introduced to the gene. The ERT2 moiety retains the Cre recombinase in the cytoplasm until tamoxifen administration releases this inhibition, thus permitting the recombination of genomic loxP sites. Efficient tamoxifen-induced Cre-mediated recombination throughout the body has been demonstrated through crosses with a Cre-responsive beta galactosidase reporter strain. This strain enables temporal control of floxed gene expression in vivo and is reportedly more sensitive to tamoxifen than Stock No. 004847. Homozygotes are viable and fertile.Development
A conditional Cre-ERT2 (estrogen receptor T2) cassette was inserted to intron 1 of the gene. The mutation was created in 129S4/SvJae-derived J1 embryonic stem (ES) cells and backcrossed 8 times to C57BL/6 by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (130 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cancer Research
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Inducible
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1(cre/ERT2)Tyj | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Tyler Jacks | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Cre-ERT2; Gt(ROSA)26Sortm1(cre/ESR1)Tyj; R26-Cre-ERT2; Rosa26CreER; | ||
| Mutation Made By | Dr. Tyler Jacks, Massachusetts Institute of Technology | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Site of Expression | Efficient tamoxifen-induced Cre-mediated recombination throughout the body has been demonstrated through crosses with a Cre-responsive beta galactosidase reporter strain. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Driver Note | Gt(ROSA)26Sor | ||
| Inducible Note | induced by tamoxifen | ||
| Molecular Note | A cassette containing Cre-ERT2 was inserted into the locus. [MGI Ref ID J:118233] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(cre/ERT2)Tyj, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Ventura A; Kirsch DG; McLaughlin ME; Tuveson DA; Grimm J; Lintault L; Newman J; Reczek EE; Weissleder R; Jacks T. 2007. Restoration of p53 function leads to tumour regression in vivo. Nature 445(7128):661-5. [PubMed: 17251932] [MGI Ref ID J:118233]
Additional References
Gt(ROSA)26Sortm1(cre/ERT2)Tyj relatedBjerknes M; Khandanpour C; Moroy T; Fujiyama T; Hoshino M; Klisch TJ; Ding Q; Gan L; Wang J; Martin MG; Cheng H. 2012. Origin of the brush cell lineage in the mouse intestinal epithelium. Dev Biol 362(2):194-218. [PubMed: 22185794] [MGI Ref ID J:180770]
Bourgo RJ; Ehmer U; Sage J; Knudsen ES. 2011. RB deletion disrupts coordination between DNA replication licensing and mitotic entry in vivo. Mol Biol Cell 22(7):931-9. [PubMed: 21289097] [MGI Ref ID J:183003]
Brady CA; Jiang D; Mello SS; Johnson TM; Jarvis LA; Kozak MM; Kenzelmann Broz D; Basak S; Park EJ; McLaughlin ME; Karnezis AN; Attardi LD. 2011. Distinct p53 transcriptional programs dictate acute DNA-damage responses and tumor suppression. Cell 145(4):571-83. [PubMed: 21565614] [MGI Ref ID J:173395]
Chen H; Gu X; Liu Y; Wang J; Wirt SE; Bottino R; Schorle H; Sage J; Kim SK. 2011. PDGF signalling controls age-dependent proliferation in pancreatic beta-cells. Nature 478(7369):349-55. [PubMed: 21993628] [MGI Ref ID J:177411]
Fragoso R; Mao T; Wang S; Schaffert S; Gong X; Yue S; Luong R; Min H; Yashiro-Ohtani Y; Davis M; Pear W; Chen CZ. 2012. Modulating the strength and threshold of NOTCH oncogenic signals by mir-181a-1/b-1. PLoS Genet 8(8):e1002855. [PubMed: 22916024] [MGI Ref ID J:188125]
Greter M; Helft J; Chow A; Hashimoto D; Mortha A; Agudo-Cantero J; Bogunovic M; Gautier EL; Miller J; Leboeuf M; Lu G; Aloman C; Brown BD; Pollard JW; Xiong H; Randolph GJ; Chipuk JE; Frenette PS; Merad M. 2012. GM-CSF controls nonlymphoid tissue dendritic cell homeostasis but is dispensable for the differentiation of inflammatory dendritic cells. Immunity 36(6):1031-46. [PubMed: 22749353] [MGI Ref ID J:187407]
Greter M; Lelios I; Pelczar P; Hoeffel G; Price J; Leboeuf M; Kundig TM; Frei K; Ginhoux F; Merad M; Becher B. 2012. Stroma-derived interleukin-34 controls the development and maintenance of langerhans cells and the maintenance of microglia. Immunity 37(6):1050-60. [PubMed: 23177320] [MGI Ref ID J:191069]
Haigis KM; Kendall KR; Wang Y; Cheung A; Haigis MC; Glickman JN; Niwa-Kawakita M; Sweet-Cordero A; Sebolt-Leopold J; Shannon KM; Settleman J; Giovannini M; Jacks T. 2008. Differential effects of oncogenic K-Ras and N-Ras on proliferation, differentiation and tumor progression in the colon. Nat Genet 40(5):600-8. [PubMed: 18372904] [MGI Ref ID J:132357]
Hoshii T; Tadokoro Y; Naka K; Ooshio T; Muraguchi T; Sugiyama N; Soga T; Araki K; Yamamura K; Hirao A. 2012. mTORC1 is essential for leukemia propagation but not stem cell self-renewal. J Clin Invest 122(6):2114-29. [PubMed: 22622041] [MGI Ref ID J:190434]
Jiang D; Brady CA; Johnson TM; Lee EY; Park EJ; Scott MP; Attardi LD. 2011. Full p53 transcriptional activation potential is dispensable for tumor suppression in diverse lineages. Proc Natl Acad Sci U S A 108(41):17123-8. [PubMed: 21969549] [MGI Ref ID J:177455]
Kaiser WJ; Upton JW; Long AB; Livingston-Rosanoff D; Daley-Bauer LP; Hakem R; Caspary T; Mocarski ES. 2011. RIP3 mediates the embryonic lethality of caspase-8-deficient mice. Nature 471(7338):368-72. [PubMed: 21368762] [MGI Ref ID J:170815]
Leite de Oliveira R; Deschoemaeker S; Henze AT; Debackere K; Finisguerra V; Takeda Y; Roncal C; Dettori D; Tack E; Jonsson Y; Veschini L; Peeters A; Anisimov A; Hofmann M; Alitalo K; Baes M; D'hooge J; Carmeliet P; Mazzone M. 2012. Gene-targeting of Phd2 improves tumor response to chemotherapy and prevents side-toxicity. Cancer Cell 22(2):263-77. [PubMed: 22897855] [MGI Ref ID J:191820]
Meylan E; Dooley AL; Feldser DM; Shen L; Turk E; Ouyang C; Jacks T. 2009. Requirement for NF-kappaB signalling in a mouse model of lung adenocarcinoma. Nature 462(7269):104-7. [PubMed: 19847165] [MGI Ref ID J:154041]
Mu P; Han YC; Betel D; Yao E; Squatrito M; Ogrodowski P; de Stanchina E; D'Andrea A; Sander C; Ventura A. 2009. Genetic dissection of the miR-17~92 cluster of microRNAs in Myc-induced B-cell lymphomas. Genes Dev 23(24):2806-11. [PubMed: 20008931] [MGI Ref ID J:155803]
Nguyen AT; Taranova O; He J; Zhang Y. 2011. DOT1L, the H3K79 methyltransferase, is required for MLL-AF9-mediated leukemogenesis. Blood 117(25):6912-22. [PubMed: 21521783] [MGI Ref ID J:174817]
Pajcini KV; Corbel SY; Sage J; Pomerantz JH; Blau HM. 2010. Transient inactivation of Rb and ARF yields regenerative cells from postmitotic mammalian muscle. Cell Stem Cell 7(2):198-213. [PubMed: 20682446] [MGI Ref ID J:163316]
Ripoche D; Gout J; Pommier RM; Jaafar R; Zhang CX; Bartholin L; Bertolino P. 2012. Generation of a conditional mouse model to target Acvr1b disruption in adult tissues. Genesis :. [PubMed: 23109354] [MGI Ref ID J:188629]
Schwab KR; Smith GD; Dressler GR. 2013. Arrested spermatogenesis and evidence for DNA damage in PTIP mutant testes. Dev Biol 373(1):64-71. [PubMed: 23063797] [MGI Ref ID J:190993]
Sfeir A; de Lange T. 2012. Removal of shelterin reveals the telomere end-protection problem. Science 336(6081):593-7. [PubMed: 22556254] [MGI Ref ID J:184522]
Unnisa Z; Clark JP; Roychoudhury J; Thomas E; Tessarollo L; Copeland NG; Jenkins NA; Grimes HL; Kumar AR. 2012. Meis1 preserves hematopoietic stem cells in mice by limiting oxidative stress. Blood 120(25):4973-81. [PubMed: 23091297] [MGI Ref ID J:192624]
Viatour P; Somervaille TC; Venkatasubrahmanyam S; Kogan S; McLaughlin ME; Weissman IL; Butte AJ; Passegue E; Sage J. 2008. Hematopoietic stem cell quiescence is maintained by compound contributions of the retinoblastoma gene family. Cell Stem Cell 3(4):416-28. [PubMed: 18940733] [MGI Ref ID J:149791]
Wang J; Kumar RM; Biggs VJ; Lee H; Chen Y; Kagey MH; Young RA; Abate-Shen C. 2011. The Msx1 Homeoprotein Recruits Polycomb to the Nuclear Periphery during Development. Dev Cell 21(3):575-88. [PubMed: 21852201] [MGI Ref ID J:178355]
Wang NS; McHeyzer-Williams LJ; Okitsu SL; Burris TP; Reiner SL; McHeyzer-Williams MG. 2012. Divergent transcriptional programming of class-specific B cell memory by T-bet and RORalpha. Nat Immunol 13(6):604-11. [PubMed: 22561605] [MGI Ref ID J:186447]
Wong IP; Driessler F; Khor EC; Shi YC; Hormer B; Nguyen AD; Enriquez RF; Eisman JA; Sainsbury A; Herzog H; Baldock PA. 2012. Peptide YY regulates bone remodeling in mice: a link between gut and skeletal biology. PLoS One 7(7):e40038. [PubMed: 22792209] [MGI Ref ID J:189512]
Yao R; Natsume Y; Saiki Y; Shioya H; Takeuchi K; Yamori T; Toki H; Aoki I; Saga T; Noda T. 2012. Disruption of Tacc3 function leads to in vivo tumor regression. Oncogene 31(2):135-48. [PubMed: 21685933] [MGI Ref ID J:179414]
Young NP; Crowley D; Jacks T. 2011. Uncoupling Cancer Mutations Reveals Critical Timing of p53 Loss in Sarcomagenesis. Cancer Res 71(11):4040-7. [PubMed: 21512139] [MGI Ref ID J:172206]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, homozygotes or heterozygotes may be bred. Mating System Homozygote x Homozygote (Female x Male) 16-DEC-09 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(cre/ERT2)Tyj
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Gt(ROSA)26Sortm1(cre/ERT2)Tyj x Homozygous for Gt(ROSA)26Sortm1(cre/ERT2)Tyj Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Gt(ROSA)26Sortm1(cre/ERT2)Tyj
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Gt(ROSA)26Sortm1(cre/ERT2)Tyj x Homozygous for Gt(ROSA)26Sortm1(cre/ERT2)Tyj Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
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|
|
Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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