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| These Wnt9bc mice harbor loxP sites flanking exon 2 of the Wnt9b (wingless-type MMTV integration site 9B) locus and may be useful in studying the role of Wnt9b (and other Wnt family members) in development and canonical Wnt signaling cascades, including metanephric kidney and urogenital system development. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6.129X1(Cg)-Wnt9btm1.2Amc/J (Changed: 09-JUL-09 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N6pN1
Generation DefinitionsDonating Investigator Andrew P McMahon, University of Southern California Description
Mice homozygous for the Wnt9bc allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). Such deletion is predicted to result in out-of-frame splicing of exon 1 to exon 3, and consequently a mutant transcript that would encode a nonfunctional peptide comprising the first 27 amino acids of Wnt9b that includes the signal peptide. These Wnt9bc mice may be useful in generating conditional mutations for studying the role of Wnt9b (and other Wnt family members) in development and canonical Wnt signaling cascades, including metanephric kidney and urogenital system development.Development
A targeting vector was designed to insert a loxP site just upstream of exon 2, and a frt-flanked PGK-Neo cassette followed by a second loxP site just downstream of exon 2 of the targeted gene. This construct was microinjected into 129X1/SvJ-derived AV3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred to C57BL/6 inbred mice to generate Wnt9bcneo mice. To remove the frt-flanked selection cassette, Wnt9bcneo mice were then bred to an FLPe-expressing strain (on the Swiss-Webster genetic background). The resulting Wnt9bc offspring (with a loxP site just upstream of exon 2, and a single remaining frt-site followed by a second loxP site just downstream of exon 2) were bred with C57BL/6 mice for approximately 6 generations prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony. A SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed 17 of 27 markers that were not C57BL/6 allele-type, suggesting this strain is still segregating for Swiss Webster markers.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Facebase: models
View Facebase: models (58 strains)
000645 A/HeJ 000646 A/J 000647 A/WySnJ 001690 AXB23/PgnJ
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Developmental Biology Research
Internal/Organ Defects
kidney
Internal/Organ Research
Kidney Defects
Research Tools
Cre-lox System
loxP-flanked Sequences
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Reproductive Biology Research
Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Wnt9btm1.2Amc | ||
|---|---|---|---|
| Allele Name | targeted mutation 1.2, Andrew P McMahon | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | Wnt9bc; Wnt9bflox; | ||
| Mutation Made By | Andrew McMahon, University of Southern California | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Name | AV3 | ||
| ES Cell Line Strain | 129X1/SvJ | ||
| Gene Symbol and Name | Wnt9b, wingless-type MMTV integration site 9B | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | WNT14B; WNT15; Wnt15; cleft lip; cleft lip 1; clf; clf1; wingless-type MMTV integration site 15; | ||
| Molecular Note | A targeting vector was designed to insert a loxP site just upstream of exon 2, and a frt-flanked PGK-Neo cassette followed by a second loxP site just downstream of exon 2 of the targeted gene to generate Wnt9bMtm1Amc mice (Wnt9bcneo. To remove the frt-flanked selection cassette, these mice were then bred to an FLPe-expressing strain (on the Swiss-Webster genetic background). The resulting Wnt9bc offspring carry a loxP site just upstream of exon 2, and a single remaining frt-site followed by a second loxP site just downstream of exon 2. [MGI Ref ID J:100575] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Wnt9btm1.2Amc, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Carroll TJ; Park JS; Hayashi S; Majumdar A; McMahon AP. 2005. Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. Dev Cell 9(2):283-92. [PubMed: 16054034] [MGI Ref ID J:100575]
Additional References
Wnt9btm1.2Amc relatedJin YR; Han XH; Taketo MM; Yoon JK. 2012. Wnt9b-dependent FGF signaling is crucial for outgrowth of the nasal and maxillary processes during upper jaw and lip development. Development 139(10):1821-30. [PubMed: 22461561] [MGI Ref ID J:184014]
Karner CM; Chirumamilla R; Aoki S; Igarashi P; Wallingford JB; Carroll TJ. 2009. Wnt9b signaling regulates planar cell polarity and kidney tubule morphogenesis. Nat Genet 41(7):793-9. [PubMed: 19543268] [MGI Ref ID J:151498]
Karner CM; Das A; Ma Z; Self M; Chen C; Lum L; Oliver G; Carroll TJ. 2011. Canonical Wnt9b signaling balances progenitor cell expansion and differentiation during kidney development. Development 138(7):1247-57. [PubMed: 21350016] [MGI Ref ID J:171512]
Mugford JW; Yu J; Kobayashi A; McMahon AP. 2009. High-resolution gene expression analysis of the developing mouse kidney defines novel cellular compartments within the nephron progenitor population. Dev Biol 333(2):312-23. [PubMed: 19591821] [MGI Ref ID J:152419]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2450.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3185.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
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| phone: | 207-288-6470 |
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