Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Heterozygote         (Female x Male)   16-MAY-09 Species laboratory mouse Generation N11+ (15-MAY-09)   Donating Investigator W. Scott Young III,   National Institute of Mental Health

Description
Mice homozygous for the Oxtr^flox allele are viable and fertile, with loxP sites flanking exons 2-3 of the targeted gene. Expression and receptor binding distributions from the Oxtr^flox targeted allele are reported to be normal when compared to wild-type. When Oxtr^flox mice are bred to mice that express Cre recombinase, the resulting offspring will have the floxed region deleted in the cre-expressing tissue(s). These Oxtr^flox mice may be used for spatial and temporal inactivation of the oxytocin receptor in studying (for example) parturition and lactation, as well as social, behavioral, and learning disorders such as autism and anxiety.

These Oxtr^flox mice may also be useful along with the Oxt/EGFP AI03 transgenic mice (Stock No. 006043) or oxytocin targeted mutant mice (Stock No. 002713) from the same donating investigator.

Development
A targeting vector was designed to place a frt-flanked PGK-Neo cassette followed by a loxP site within intron 1, and a second loxP site within intron 3 of the targeted gene. The targeting construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells, and ES cells were injected into recipient blastocysts to generate chimeric mice. The donating investigator reports that chimeric mice were bred to C57BL/6 to establish Oxtr^Neo mutant mice. To remove the selection cassette, these Oxtr^Neo mice were bred to FLPe-expressing mice (on a B6;SJL genetic background, see Stock No. 003800). The resulting Oxtr^flox offspring (with a single frt site remaining upstream of the loxP-flanked exons 2-3) were then selectively bred to remove the transgene by backcrossing to C57BL/6J inbred mice for at least 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J for at least one generation to establish the colony.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Oxtr^tm1.1Wsy/Oxtr^tm1.1Wsy

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

• normal phenotype
• no abnormal phenotype detected (MGI Ref ID J:137445)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects
Cre-lox System
      loxP-flanked Sequences
Neurodevelopmental Defects
      Autism
Receptor Defects
      G protein-coupled receptor

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Neurobiology Research
Reproductive Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information

Allele Symbol		Oxtrtm1.1Wsy
Allele Name		targeted mutation 1.1, W Scott Young III
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		Oxtrflox;
Mutation Made By		W. Scott Young III,   National Institute of Mental Health
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Oxtr, oxytocin receptor
Chromosome		6
Gene Common Name(s)		OT-R; OTR; OTR1;
Molecular Note		Germ line, flp-mediated recombination was used to remove the neo cassette leaving exons 2 and 3 floxed. Autoradiography confirmed wild-type protein expression. [MGI Ref ID J:137445]

Genotyping

Genotyping Information

Genotyping Protocols

Oxtr^tm1.1Wsy, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Lee HJ; Caldwell HK; Macbeth AH; Tolu SG; Young WS rd. 2008. A conditional knockout mouse line of the oxytocin receptor. Endocrinology 149(7):3256-63. [PubMed: 18356275]  [MGI Ref ID J:137445]

Additional References

Oxtr^tm1.1Wsy related

Macbeth AH; Lee HJ; Edds J; Young WS rd. 2009. Oxytocin and the oxytocin receptor underlie intrastrain, but not interstrain, social recognition. Genes Brain Behav 8(5):558-67. [PubMed: 19531157]  [MGI Ref ID J:151090]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           MGL375/MGL377

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred. The donating investigator breeds heterozygotes with C57BL/6J inbred mice.
Mating System	Heterozygote x Heterozygote         (Female x Male)   16-MAY-09
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

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Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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