Strain Name:

B6;SJL-Tg(THY1-SNCA*A30P)M30Sud/J

Stock Number:

008473

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
This strain carries an A30P mutant form of the human SNCA (synuclein, alpha) gene associated with the development of familial Parkinson's disease. The onset of hind limb mobility problems and a resting tremor phenotype occur around 10 months of age due to a loss of motor neurons. No Lewy body-like pathology has been observed. Extensive cell death in the spinal cord and brain are seen. This strain may be useful in studies of Parkinson's disease. Hemizygous mice are viable and fertile, and survive to approximately 14 months of age.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.Cg-Tg(THY1-SNCA*A30P)M30Sud/J    (Changed: 29-OCT-09 )
Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Thomas Sudhof,   Stanford University School of Medicine

Description
The A30P mutation in this transgenic strain is associated with the development of familial Parkinson's disease. The onset of hind limb mobility problems and a resting tremor phenotype occur around 10 months of age due to a loss of motor neurons. No Lewy body-like pathology has been observed. Extensive cell death in the spinal cord and brain are seen. This strain may be useful in studies of Parkinson's disease. Expression of the transgene is 5-fold higher in the brain and 10-fold higher in the spinal cord. Hemizygous mice are viable and fertile, and survive to approximately 14 months of age.

Development
This transgenic strain carries the human A30P mutant SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. The transgenic vector was injected into B6SJLF1/J embryos, but this line has been backcrossed approximately 10 times to C57BL/6.This is line M30.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of SNCA     (5 strains)

View Strains carrying other alleles of THY1     (5 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(THY1-SNCA*A30P)M30Sud/0

        involves: C57BL/6 * SJL
  • nervous system phenotype
  • alpha-synuclein inclusion body (MGI Ref ID J:115193)
    • insoluble alpha-synuclein aggregates form in asymptomatic and symptomatic transgenic mice in terms of neuropathology
  • decreased motor neuron number (MGI Ref ID J:115193)
  • motor neuron degeneration (MGI Ref ID J:115193)
    • mice show onset of motoneuron disease at ~9-10 months

Tg(THY1-SNCA*A30P)M30Sud/0

        Background Not Specified
  • life span-post-weaning/aging
  • *normal* life span-post-weaning/aging (MGI Ref ID J:139867)
    • mice survive to approximately 14 months
  • behavior/neurological phenotype
  • paresis (MGI Ref ID J:139867)
    • onset of hindlimb mobility problems is observed around 10 months of age due to motor neuron loss
  • tremors (MGI Ref ID J:139867)
    • resting tremors are detected in mice at about 10 months of age, resulting from motor neuron loss
  • nervous system phenotype
  • *normal* nervous system phenotype (MGI Ref ID J:139867)
    • no Lewy body-like pathology is observed in transgenic animals
    • increased neuron apoptosis (MGI Ref ID J:139867)
      • extensive cell death is observed in spinal cord and brain in adult mice
    • motor neuron degeneration (MGI Ref ID J:139867)
      • loss of motor neurons with age
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Neurodegeneration
Parkinson's Disease
      synuclein mutants

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(THY1-SNCA*A30P)M30Sud
Allele Name transgene insertion M30, Thomas C Sudhof
Allele Type Transgenic (random, expressed)
Common Name(s) M30;
Mutation Made By Thomas Sudhof,   Stanford University School of Medicine
Strain of Origin(C57BL/6J x SJL/J)F1
Expressed Gene SNCA, synuclein, alpha (non A4 component of amyloid precursor), human
Promoter THY1, Thy-1 cell surface antigen, human
Molecular Note The transgene construct contains the human A30P mutant SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. Expression of the transgene is 5-fold higher in the brain and 10-fold higher in the spinal cord. [MGI Ref ID J:115193]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(THY1-SNCA*A53T)M53Sud, F53Sud, Melt Curve Analysis
Tg(THY1-SNCA*A30P)M30Sud, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Chandra S; Gallardo G; Fernandez-Chacon R; Schluter OM; Sudhof TC. 2005. Alpha-synuclein cooperates with CSPalpha in preventing neurodegeneration. Cell 123(3):383-96. [PubMed: 16269331]  [MGI Ref ID J:115193]

Additional References

Tg(THY1-SNCA*A30P)M30Sud related

Sudhof TC. 2008. Generation of a mutant human alpha-synuclein transgene MGI Direct Data Submission :.  [MGI Ref ID J:139867]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, hemizygotes may be bred with wildtype or C57BL/6 mice.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Price (US dollars $)
Cryorecovery Fee $1900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Price (US dollars $)
Cryorecovery Fee $2470.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Supply Details

Standard SupplyCryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

  • This strain is included in the Induced Mutant Resource Colony collection.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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