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| These mice are transgenic for the A53T mutation of the human SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. Hemizygotes are viable and fertile and develop a Parkinson-like phenotype upon aging. Hind limb paralysis due to loss of motor neurons and a resting tremor are initially seen at about eight months of age. No Lewy body-like pathology is noted. Cell death in the spinal cord (extensive) and brain are observed. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg-Tg(THY1-SNCA*A53T)F53Sud/J (Changed: 17-DEC-08 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 25-OCT-08 Species laboratory mouse Generation N2+F1 (18-NOV-09) Donating Investigator Thomas Sudhof, Stanford University School of Medicine Description
These mice are transgenic for the A53T mutation of the human SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. Hemizygotes are viable and fertile and develop a Parkinson-like phenotype upon aging. Hind limb paralysis due to loss of motor neurons and a resting tremor are initially seen at about eight months of age. No Lewy body-like pathology is noted. Cell death in the spinal cord (extensive) and brain are observed. Expression of the transgene is 10-fold increased in the brain and 20-fold in the spinal cord.Development
This transgenic strain carries the human SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. It is not known what strain the transgenic vector was originally injected into, but this line has been backcrossed approximately 5 times to C57BL/6. SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that this strain is on a mixed genetic background (eight out of 27 markers are segregating for non-C57BL/6 alleles - December, 2008). This is line F53.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of SNCA
008134 B6.Cg-Tg(THY1-SNCA*A30P)TS2Sud/J 008135 B6.Cg-Tg(THY1-SNCA*A53T)M53Sud/J 004479 B6;C3-Tg(Prnp-SNCA*A53T)83Vle/J 008473 B6;SJL-Tg(THY1-SNCA*A30P)M30Sud/J 008389 C57BL/6-Tg(THY1-SNCA)1Sud/J View Strains carrying other alleles of SNCA (5 strains)
008134 B6.Cg-Tg(THY1-SNCA*A30P)TS2Sud/J 008135 B6.Cg-Tg(THY1-SNCA*A53T)M53Sud/J 008473 B6;SJL-Tg(THY1-SNCA*A30P)M30Sud/J 008389 C57BL/6-Tg(THY1-SNCA)1Sud/J 008132 STOCK Tg(THY1-Snca)M1mSud/J
Phenotype
Phenotype Information
assigned by genotype
Tg(THY1-SNCA*A53T)F53Sud/0
involves: C57BL/6
- behavior/neurological phenotype
- hindlimb paralysis (MGI Ref ID J:140019)
- initially observed around 8 months of age
- tremors (MGI Ref ID J:140019)
- resting tremor initially observed around 8 months of age
- nervous system phenotype
- increased neuron apoptosis (MGI Ref ID J:140019)
- cell death is seen in spinal cord and is extensive in brain
- motor neuron degeneration (MGI Ref ID J:140019)
- results in hindlimb paralysis around 8 months of age
This mouse can be used to support research in many areas including:
Neurobiology Research
Neurodegeneration
Parkinson's Disease
synuclein mutants
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(THY1-SNCA*A53T)F53Sud | ||
|---|---|---|---|
| Allele Name | transgenic insertion F53, Thomas C Sudhof | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | F53; | ||
| Mutation Made By | Thomas Sudhof, Stanford University School of Medicine | ||
| Expressed Gene | SNCA, synuclein, alpha (non A4 component of amyloid precursor), human | ||
| Promoter | THY1, Thy-1 cell surface antigen, human | ||
| Molecular Note | The transgenic construct carries the A53T mutant form of the human SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. Expression of the transgene is 10-fold increased in the brain and 20-fold in the spinal cord. [MGI Ref ID J:140019] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(THY1-SNCA*A53T)M53Sud, F53Sud, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Tg(THY1-SNCA*A53T)F53Sud relatedSudhof TC. 2008. Generation of a mutant A53T human alpha-synuclein transgene MGI Direct Data Submission :. [MGI Ref ID J:140019]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes may be bred to wildtype mice. Mating System Noncarrier x Hemizygote (Female x Male) 25-OCT-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(THY1-SNCA*A53T)F53Sud
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(THY1-SNCA*A53T)F53Sud x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(THY1-SNCA*A53T)F53Sud
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(THY1-SNCA*A53T)F53Sud
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(THY1-SNCA*A53T)F53Sud x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(THY1-SNCA*A53T)F53Sud
Additional Supply Details
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Supply Details
| Standard Supply | Under Development for Distribution Colony |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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