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| These transgenic mice express beta-galactosidase (lacZ) gene under the control of the retinoic acid responsive element (RARE). Retinoic acid signaling is indicated by beta-galactosidase activity. This mutant mouse strain may be useful in tracking retinoic acid signaling pattern. | |||||||||||||||
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Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 05-FEB-09 Species laboratory mouse Generation F?+F6 (16-MAR-11)
Generation DefinitionsDonating Investigator Dr. Janet Rossant, The Hospital for Sick Children Description
These transgenic mice express beta-galactosidase (lacZ) gene under the control of the retinoic acid responsive element (RARE). Sporadic beta-galactosidase activity is detected in less than half of embryonic day 3.5 blastocysts. Beta-galactosidase activity in embryos 11.5 embryonic days in age and older mimics the expression pattern of retinoic acid receptor, beta (Rarb). Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When maintained as homozygotes, this strain can lose beta-galactosidase staining capacity. The donating investigator reports that lacZ staining is lost when their mice are bred onto the C57BL/6 genetic background. This strain serves as a reporter strain, with retinoic acid signaling being indicated by beta-galactosidase activity. This mutant mouse strain may be useful in tracking retinoic acid signaling pattern.Development
A transgenic construct containing 3 copies of the 32bp retinoic acid responsive element (RARE) placed upstream of the mouse heat shock protein 1B (Hspa1b) promoter and beta-galactosidase gene (lacZ) was injected into fertilized CD1 mouse eggs. Founder line 12 was subsequently established. The mice were maintained on the outbred ICR background.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (255 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(RARE-Hspa1b/lacZ)12Jrt/0
involves: CD-1
- no phenotypic analysis
- *normal* no phenotypic analysis
- mice were used for expression analysis only (MGI Ref ID J:135403)
This mouse can be used to support research in many areas including:
Research Tools
lacZ Expression
Developmental Biology Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(RARE-Hspa1b/lacZ)12Jrt | ||
|---|---|---|---|
| Allele Name | transgene insertion 12, Janet Rossant | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | RARE-hsp68-lacZ; RARE-hsp68LacZ; RARE-lacZ; | ||
| Mutation Made By | Dr. Janet Rossant, The Hospital for Sick Children | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | Sporadic beta-galactosidase activity is detected in less than half of embryonic day 3.5 blastocysts. Beta-galactosidase activity in embryos 11.5 embryonic days in age and older mimics the expression pattern of retinoic acid receptor, beta (Rarb). | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | RARE, retinoic acid responsive element, | ||
| Molecular Note | Three copies of the 32 bp RARE found upstream of RARB were inserted 5' to a DNA fragment composed of a fragment of the Hspa1b gene containin 610 bp upstream of the transcription start site as well as 225 bp of sequence containing the translational start site. This was fused in frame to a lacZ gene. Expression for this transgene line was identical to that found in lines 63 and 65. [MGI Ref ID J:109146] | ||
| Gene Symbol and Name | Tg(RARE-Hspa1b/lacZ)12Jrt, transgene insertion 12, Janet Rossant | ||
| Chromosome | UN | ||
| Gene Common Name(s) | RARE-hsp68LacZ; RARE-lacZ; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ Melt Curve Analysis, Melt Curve Analysis
Generic LacZ QPCR, QPCR
Generic LacZ, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Rajaii F; Bitzer ZT; Xu Q; Sockanathan S. 2008. Expression of the dominant negative retinoid receptor, RAR403, alters telencephalic progenitor proliferation, survival, and cell fate specification. Dev Biol 316(2):371-82. [PubMed: 18329011] [MGI Ref ID J:135403]
Rossant J; Zirngibl R; Cado D; Shago M; Giguere V. 1991. Expression of a retinoic acid response element-hsplacZ transgene defines specific domains of transcriptional activity during mouse embryogenesis. Genes Dev 5(8):1333-44. [PubMed: 1907940] [MGI Ref ID J:109146]
Additional References
Tg(RARE-Hspa1b/lacZ)12Jrt relatedAlfano G; Conte I; Caramico T; Avellino R; Arno B; Pizzo MT; Tanimoto N; Beck SC; Huber G; Dolle P; Seeliger MW; Banfi S. 2011. Vax2 regulates retinoic acid distribution and cone opsin expression in the vertebrate eye. Development 138(2):261-71. [PubMed: 21148184] [MGI Ref ID J:167740]
Bertrand N; Roux M; Ryckebusch L; Niederreither K; Dolle P; Moon A; Capecchi M; Zaffran S. 2011. Hox genes define distinct progenitor sub-domains within the second heart field. Dev Biol 353(2):266-74. [PubMed: 21385575] [MGI Ref ID J:173839]
Borello U; Cobos I; Long JE; McWhirter JR; Murre C; Rubenstein JL. 2008. FGF15 promotes neurogenesis and opposes FGF8 function during neocortical development. Neural Dev 3:17. [PubMed: 18625063] [MGI Ref ID J:160580]
Cammas L; Trensz F; Jellali A; Ghyselinck NB; Roux MJ; Dolle P. 2010. Retinoic acid receptor (RAR)-alpha is not critically required for mediating retinoic acid effects in the developing mouse retina. Invest Ophthalmol Vis Sci 51(6):3281-90. [PubMed: 20107170] [MGI Ref ID J:165076]
Chen F; Cao Y; Qian J; Shao F; Niederreither K; Cardoso WV. 2010. A retinoic acid-dependent network in the foregut controls formation of the mouse lung primordium. J Clin Invest 120(6):2040-8. [PubMed: 20484817] [MGI Ref ID J:161637]
Clugston RD; Zhang W; Alvarez S; de Lera AR; Greer JJ. 2010. Understanding abnormal retinoid signaling as a causative mechanism in congenital diaphragmatic hernia. Am J Respir Cell Mol Biol 42(3):276-85. [PubMed: 19448158] [MGI Ref ID J:171491]
Colvin EK; Susanto JM; Kench JG; Ong VN; Mawson A; Pinese M; Chang DK; Rooman I; O'Toole SA; Segara D; Musgrove EA; Sutherland RL; Apte MV; Scarlett CJ; Biankin AV. 2011. Retinoid signaling in pancreatic cancer, injury and regeneration. PLoS One 6(12):e29075. [PubMed: 22220202] [MGI Ref ID J:182325]
Cunningham TJ; Chatzi C; Sandell LL; Trainor PA; Duester G. 2011. Rdh10 mutants deficient in limb field retinoic acid signaling exhibit normal limb patterning but display interdigital webbing. Dev Dyn 240(5):1142-50. [PubMed: 21360789] [MGI Ref ID J:171078]
Cunningham TJ; Zhao X; Duester G. 2011. Uncoupling of retinoic acid signaling from tailbud development before termination of body axis extension. Genesis 49(10):776-83. [PubMed: 21538808] [MGI Ref ID J:178365]
Faedo A; Tomassy GS; Ruan Y; Teichmann H; Krauss S; Pleasure SJ; Tsai SY; Tsai MJ; Studer M; Rubenstein JL. 2008. COUP-TFI coordinates cortical patterning, neurogenesis, and laminar fate and modulates MAPK/ERK, AKT, and beta-catenin signaling. Cereb Cortex 18(9):2117-31. [PubMed: 18165280] [MGI Ref ID J:146820]
Ji SJ; Zhuang B; Falco C; Schneider A; Schuster-Gossler K; Gossler A; Sockanathan S. 2006. Mesodermal and neuronal retinoids regulate the induction and maintenance of limb innervating spinal motor neurons. Dev Biol 297(1):249-61. [PubMed: 16781703] [MGI Ref ID J:112635]
Lee YJ; McPherron A; Choe S; Sakai Y; Chandraratna RA; Lee SJ; Oh SP. 2010. Growth differentiation factor 11 signaling controls retinoic acid activity for axial vertebral development. Dev Biol 347(1):195-203. [PubMed: 20801112] [MGI Ref ID J:165715]
Li P; Pashmforoush M; Sucov HM. 2010. Retinoic acid regulates differentiation of the secondary heart field and TGFbeta-mediated outflow tract septation. Dev Cell 18(3):480-5. [PubMed: 20230754] [MGI Ref ID J:159110]
Lin SC; Dolle P; Ryckebusch L; Noseda M; Zaffran S; Schneider MD; Niederreither K. 2010. Endogenous retinoic acid regulates cardiac progenitor differentiation. Proc Natl Acad Sci U S A 107(20):9234-9. [PubMed: 20439714] [MGI Ref ID J:160288]
Matson CK; Murphy MW; Griswold MD; Yoshida S; Bardwell VJ; Zarkower D. 2010. The mammalian doublesex homolog DMRT1 is a transcriptional gatekeeper that controls the mitosis versus meiosis decision in male germ cells. Dev Cell 19(4):612-24. [PubMed: 20951351] [MGI Ref ID J:166057]
Mic FA; Haselbeck RJ; Cuenca AE; Duester G. 2002. Novel retinoic acid generating activities in the neural tube and heart identified by conditional rescue of Raldh2 null mutant mice. Development 129(9):2271-82. [PubMed: 11959834] [MGI Ref ID J:75950]
Moss JB; Xavier-Neto J; Shapiro MD; Nayeem SM; McCaffery P; Drager UC; Rosenthal N. 1998. Dynamic patterns of retinoic acid synthesis and response in the developing mammalian heart. Dev Biol 199(1):55-71. [PubMed: 9676192] [MGI Ref ID J:48571]
Naiche LA; Holder N; Lewandoski M. 2011. FGF4 and FGF8 comprise the wavefront activity that controls somitogenesis. Proc Natl Acad Sci U S A 108(10):4018-23. [PubMed: 21368122] [MGI Ref ID J:170831]
Norden J; Grieskamp T; Lausch E; van Wijk B; van den Hoff MJ; Englert C; Petry M; Mommersteeg MT; Christoffels VM; Niederreither K; Kispert A. 2010. Wt1 and retinoic acid signaling in the subcoelomic mesenchyme control the development of the pleuropericardial membranes and the sinus horns. Circ Res 106(7):1212-20. [PubMed: 20185795] [MGI Ref ID J:171035]
Oh S; Huang X; Liu J; Litingtung Y; Chiang C. 2009. Shh and Gli3 activities are required for timely generation of motor neuron progenitors. Dev Biol 331(2):261-9. [PubMed: 19433083] [MGI Ref ID J:150755]
Okano J; Kimura W; Papaioannou VE; Miura N; Yamada G; Shiota K; Sakai Y. 2012. The regulation of endogenous retinoic acid level through CYP26B1 is required for elevation of palatal shelves. Dev Dyn 241(11):1744-56. [PubMed: 22972661] [MGI Ref ID J:187968]
Okano J; Sakai Y; Shiota K. 2008. Retinoic acid down-regulates Tbx1 expression and induces abnormal differentiation of tongue muscles in fetal mice. Dev Dyn 237(10):3059-3070. [PubMed: 18816858] [MGI Ref ID J:139633]
Rhinn M; Schuhbaur B; Niederreither K; Dolle P. 2011. Involvement of retinol dehydrogenase 10 in embryonic patterning and rescue of its loss of function by maternal retinaldehyde treatment. Proc Natl Acad Sci U S A 108(40):16687-92. [PubMed: 21930923] [MGI Ref ID J:177362]
Ribes V; Le Roux I; Rhinn M; Schuhbaur B; Dolle P. 2009. Early mouse caudal development relies on crosstalk between retinoic acid, Shh and Fgf signalling pathways. Development 136(4):665-76. [PubMed: 19168680] [MGI Ref ID J:144016]
Rosselot C; Spraggon L; Chia I; Batourina E; Riccio P; Lu B; Niederreither K; Dolle P; Duester G; Chambon P; Costantini F; Gilbert T; Molotkov A; Mendelsohn C. 2010. Non-cell-autonomous retinoid signaling is crucial for renal development. Development 137(2):283-92. [PubMed: 20040494] [MGI Ref ID J:157254]
Ryckebusch L; Wang Z; Bertrand N; Lin SC; Chi X; Schwartz R; Zaffran S; Niederreither K. 2008. Retinoic acid deficiency alters second heart field formation. Proc Natl Acad Sci U S A 105(8):2913-8. [PubMed: 18287057] [MGI Ref ID J:132815]
Sandell LL; Sanderson BW; Moiseyev G; Johnson T; Mushegian A; Young K; Rey JP; Ma JX; Staehling-Hampton K; Trainor PA. 2007. RDH10 is essential for synthesis of embryonic retinoic acid and is required for limb, craniofacial, and organ development. Genes Dev 21(9):1113-24. [PubMed: 17473173] [MGI Ref ID J:121229]
Silvestri C; Narimatsu M; von Both I; Liu Y; Tan NB; Izzi L; McCaffery P; Wrana JL; Attisano L. 2008. Genome-wide identification of Smad/Foxh1 targets reveals a role for Foxh1 in retinoic acid regulation and forebrain development. Dev Cell 14(3):411-23. [PubMed: 18331719] [MGI Ref ID J:133339]
Sirbu IO; Duester G. 2006. Retinoic-acid signalling in node ectoderm and posterior neural plate directs left-right patterning of somitic mesoderm. Nat Cell Biol 8(3):271-7. [PubMed: 16489341] [MGI Ref ID J:129404]
Sirbu IO; Zhao X; Duester G. 2008. Retinoic acid controls heart anteroposterior patterning by down-regulating Isl1 through the Fgf8 pathway. Dev Dyn 237(6):1627-35. [PubMed: 18498088] [MGI Ref ID J:136359]
Stottmann RW; Moran JL; Turbe-Doan A; Driver E; Kelley M; Beier DR. 2011. Focusing Forward Genetics: A Tripartite ENU Screen for Neurodevelopmental Mutations in the Mouse. Genetics 188(3):615-24. [PubMed: 21515572] [MGI Ref ID J:174027]
Tong MH; Yang QE; Davis JC; Griswold MD. 2013. Retinol dehydrogenase 10 is indispensible for spermatogenesis in juvenile males. Proc Natl Acad Sci U S A 110(2):543-8. [PubMed: 23267101] [MGI Ref ID J:193281]
Uehara M; Yashiro K; Mamiya S; Nishino J; Chambon P; Dolle P; Sakai Y. 2007. CYP26A1 and CYP26C1 cooperatively regulate anterior-posterior patterning of the developing brain and the production of migratory cranial neural crest cells in the mouse. Dev Biol 302(2):399-411. [PubMed: 17067568] [MGI Ref ID J:119939]
Vitobello A; Ferretti E; Lampe X; Vilain N; Ducret S; Ori M; Spetz JF; Selleri L; Rijli FM. 2011. Hox and Pbx Factors Control Retinoic Acid Synthesis during Hindbrain Segmentation. Dev Cell 20(4):469-82. [PubMed: 21497760] [MGI Ref ID J:171613]
Wahl MB; Deng C; Lewandoski M; Pourquie O. 2007. FGF signaling acts upstream of the NOTCH and WNT signaling pathways to control segmentation clock oscillations in mouse somitogenesis. Development 134(22):4033-41. [PubMed: 17965051] [MGI Ref ID J:127075]
Wong YF; Kopp JB; Roberts C; Scambler PJ; Abe Y; Rankin AC; Dutt N; Hendry BM; Xu Q. 2011. Endogenous retinoic acid activity in principal cells and intercalated cells of mouse collecting duct system. PLoS One 6(2):e16770. [PubMed: 21326615] [MGI Ref ID J:170894]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice can be bred together or to wildtype sibling. The donating investigator reports that lacZ staining is lost when their mice are bred onto the C57BL/6 genetic background. Mating System Homozygote x Homozygote (Female x Male) 05-FEB-09 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Tg(RARE-Hspa1b/lacZ)12Jrt
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Tg(RARE-Hspa1b/lacZ)12Jrt x Homozygous for Tg(RARE-Hspa1b/lacZ)12Jrt Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Tg(RARE-Hspa1b/lacZ)12Jrt
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Tg(RARE-Hspa1b/lacZ)12Jrt x Homozygous for Tg(RARE-Hspa1b/lacZ)12Jrt Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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