| |||||||||||||||||||
| A loxP-flanked neomycin cassette blocks expression of the rat Trpv1 (transient receptor potential cation channel, subfamily V, member 1) gene driven by the Gt(ROSA)26Sor gene in this targeted mutation/knock-in strain. Upon crossing to a tissue-specific Cre-expressing strain, TRPV1 and enhanced cyan fluorescent protein (ECFP) is expressed from the ROSA locus. Cells expressing TRPV1 are sensitive to capsaicin and similar chemical agonists. Treatment of mice or cells that have undergone Cre excision to remove the neomycin cassette can induce strong inward currents, trigger action potentials and activate stereotyped behaviors, allowing cell-type specific chemical genetic control of neuronal activity in vitro and in vivo. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6;129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J (Changed: 12-FEB-09 ) B6;129P2-Gt(ROSA)26Sortm1(Trpv1-ECFP)Mde/J (Changed: 15-JUL-08 ) Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 12-JAN-10 Species laboratory mouse Generation N4+N1 (12-FEB-09)
Generation DefinitionsDonating Investigator Michael Ehlers, Duke University Medical Center, HHMI Description
A loxP-flanked neomycin cassette blocks expression of the rat Trpv1 (transient receptor potential cation channel, subfamily V, member 1) gene driven by the Gt(ROSA)26Sor gene in this targeted mutation/knock-in strain. Upon crossing to a tissue-specific Cre-expressing strain, TRPV1 and enhanced cyan fluorescent protein (ECFP) is expressed from the ROSA locus. Cells expressing TRPV1 are sensitive to capsaicin and similar chemical agonists. Treatment of mice or cells that have undergone Cre excision to remove the neomycin cassette can induce strong inward currents, trigger action potentials and activate stereotyped behaviors, allowing cell-type specific chemical genetic control of neuronal activity in vitro and in vivo. Mice that are homozygous for the floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.When bred to a strain with widespread expression of Cre recombinase (see Stock No. 002858 for example), this mutant mouse strain may be useful in studies of neuronal activity.
Development
A targeting vector was used to insert a loxP-flanked neomycin cassette, rat Trpv1 (transient receptor potential cation channel, subfamily V, member 1) cDNA, and IRES-ECFP sequence into the Gt(ROSA)26Sor gene. The mutation was created in 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were backcrossed to C57BL/6 three times by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (240 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/Gt(ROSA)26Sor+
involves: 129P2/OlaHsd * C57BL/6 (conditional)
- behavior/neurological phenotype
- circling
- capsaicin infusion after focal induction of Trpv1 expression in subsets of striatal neurons by injection of a lentiviral-cre vector recapitulates contralateral circling behavior at one week post-injection; circling subsides within 15 minutes of capsaicin application (MGI Ref ID J:136582)
- ipsilateral circling is not observed after capsaicin infusion (MGI Ref ID J:136582)
- hearing/vestibular/ear phenotype
- circling
- capsaicin infusion after focal induction of Trpv1 expression in subsets of striatal neurons by injection of a lentiviral-cre vector recapitulates contralateral circling behavior at one week post-injection; circling subsides within 15 minutes of capsaicin application (MGI Ref ID J:136582)
- ipsilateral circling is not observed after capsaicin infusion (MGI Ref ID J:136582)
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/Gt(ROSA)26Sor+ Tg(Nes-cre)1Wme/0
involves: 129P2/OlaHsd * C57BL/6 * CBA (conditional)
- behavior/neurological phenotype
- *normal* behavior/neurological phenotype
- capsaicin infusion into striatum does not affect exploratory or motor behavior in Cre-activated mutants or controls (MGI Ref ID J:136582)
- circling
- infusion of capsaicin into striatum of results in stereotyped contralateral circling behavior within 5 minutes of delivery, while control mice without activation of Trpv1 show no effect (MGI Ref ID J:136582)
- nervous system phenotype
- abnormal single cell response
- in layer 5 cortical slices, capsaicin induces action potentials while cells from Cre-negative animals show no response (MGI Ref ID J:136582)
- neurons in slices show dose-dependent depolarizing currents in response to capsaicin applied to the bath (MGI Ref ID J:136582)
- capsaicin applied acutely to single cells causes reproducible bursts of action potentials with increases in firing rate; frequency of firing is dependent on duration and concentration of capsaicin application (MGI Ref ID J:136582)
- increased susceptibility to neuronal excitotoxicity
- hearing/vestibular/ear phenotype
- circling
- infusion of capsaicin into striatum of results in stereotyped contralateral circling behavior within 5 minutes of delivery, while control mice without activation of Trpv1 show no effect (MGI Ref ID J:136582)
- homeostasis/metabolism phenotype
- increased susceptibility to neuronal excitotoxicity
This mouse can be used to support research in many areas including:
Neurobiology Research
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Research Tools
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Fluorescent Proteins
Neurobiology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde | ||
|---|---|---|---|
| Allele Name | targeted insertion 1, Michael D Ehlers | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | ROSA-stop flox-TRPV1-IRES-ECFP; | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | A floxed Neo prevents transcription of ECFP and rat Trpv1 (transient receptor potential cation channel, subfamily V, member 1)) until mice are bred to a Cre strain. Resulting offspring express ECFP in a pattern dictated by the Cre promoter. | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A targeting vector was used to insert a loxP-flanked neomycin cassette, rat Trpv1 (transient receptor potential cation channel, subfamily V, member 1) cDNA, and IRES-ECFP sequence into the Gt(ROSA)26Sor locus. [MGI Ref ID J:136582] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Arenkiel BR; Klein ME; Davison IG; Katz LC; Ehlers MD. 2008. Genetic control of neuronal activity in mice conditionally expressing TRPV1. Nat Methods 5(4):299-302. [PubMed: 18327266] [MGI Ref ID J:136582]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375/MGL377
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, heterozygotes or homozygotes may be bred. Mating System Homozygote x Homozygote (Female x Male) 12-JAN-10 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
 |
Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $160.40 Female or Male Homozygous for Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde
Pairs /Price (US dollars $) Pair Genotype $320.80 Homozygous for Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde x Homozygous for Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes
- Usually shipped between four and eight weeks of age.
| Pricing for International shipping destinations |
|
|
Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $208.60 Female or Male Homozygous for Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde
Pairs /Price (US dollars $) Pair Genotype $417.10 Homozygous for Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde x Homozygous for Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes
- Usually shipped between four and eight weeks of age.
|
|
|
Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
Contact information
General inquiriesContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.