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| A loxP-flanked neomycin cassette blocks expression of the rat Trpv1 (transient receptor potential cation channel, subfamily V, member 1) gene driven by the Gt(ROSA)26Sor gene in this targeted mutation/knock-in strain. Upon crossing to a tissue-specific Cre-expressing strain, TRPV1 and enhanced cyan fluorescent protein (ECFP) is expressed from the ROSA locus. Cells expressing TRPV1 are sensitive to capsaicin and similar chemical agonists. Treatment of mice or cells that have undergone Cre excision to remove the neomycin cassette can induce strong inward currents, trigger action potentials and activate stereotyped behaviors, allowing cell-type specific chemical genetic control of neuronal activity in vitro and in vivo. | |||||||||||
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Description
Strain Information
Former Names B6;129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J (Changed: 12-FEB-09 ) B6;129P2-Gt(ROSA)26Sortm1(Trpv1-ECFP)Mde/J (Changed: 15-JUL-08 ) Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Heterozygote (Female x Male) 13-FEB-09 Species laboratory mouse Generation N4+N1 (12-FEB-09) Donating Investigator Michael Ehlers, Duke University Medical Center, HHMI Description
A loxP-flanked neomycin cassette blocks expression of the rat Trpv1 (transient receptor potential cation channel, subfamily V, member 1) gene driven by the Gt(ROSA)26Sor gene in this targeted mutation/knock-in strain. Upon crossing to a tissue-specific Cre-expressing strain, TRPV1 and enhanced cyan fluorescent protein (ECFP) is expressed from the ROSA locus. Cells expressing TRPV1 are sensitive to capsaicin and similar chemical agonists. Treatment of mice or cells that have undergone Cre excision to remove the neomycin cassette can induce strong inward currents, trigger action potentials and activate stereotyped behaviors, allowing cell-type specific chemical genetic control of neuronal activity in vitro and in vivo. Mice that are homozygous for the floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.When bred to a strain with widespread expression of Cre recombinase (see Stock No. 002858 for example), this mutant mouse strain may be useful in studies of neuronal activity.
Development
A targeting vector was used to insert a loxP-flanked neomycin cassette, rat Trpv1 (transient receptor potential cation channel, subfamily V, member 1) cDNA, and IRES-ECFP sequence into the Gt(ROSA)26Sor gene. The mutation was created in 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were backcrossed to C57BL/6 three times by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/Gt(ROSA)26Sor+
involves: 129P2/OlaHsd * C57BL/6 (conditional)
- behavior/neurological phenotype
- circling (MGI Ref ID J:136582)
- capsaicin infusion after focal induction of Trpv1 expression in subsets of striatal neurons by injection of a lentiviral-cre vector recapitulates contralateral circling behavior at one week post-injection; circling subsides within 15 minutes of capsaicin application
- ipsilateral circling is not observed after capsaicin infusion
- hearing/vestibular/ear phenotype
- circling (MGI Ref ID J:136582)
- capsaicin infusion after focal induction of Trpv1 expression in subsets of striatal neurons by injection of a lentiviral-cre vector recapitulates contralateral circling behavior at one week post-injection; circling subsides within 15 minutes of capsaicin application
- ipsilateral circling is not observed after capsaicin infusion
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/Gt(ROSA)26Sor+ Tg(Nes-cre)1Wme/0
involves: 129P2/OlaHsd * C57BL/6 * CBA (conditional)
- behavior/neurological phenotype
- *normal* behavior/neurological phenotype (MGI Ref ID J:136582)
- capsaicin infusion into striatum does not affect exploratory or motor behavior in Cre-activated mutants or controls
- circling (MGI Ref ID J:136582)
- infusion of capsaicin into striatum of results in stereotyped contralateral circling behavior within 5 minutes of delivery, while control mice without activation of Trpv1 show no effect
- nervous system phenotype
- abnormal single cell response (MGI Ref ID J:136582)
- in layer 5 cortical slices, capsaicin induces action potentials while cells from Cre-negative animals show no response
- neurons in slices show dose-dependent depolarizing currents in response to capsaicin applied to the bath
- capsaicin applied acutely to single cells causes reproducible bursts of action potentials with increases in firing rate; frequency of firing is dependent on duration and concentration of capsaicin application
- increased susceptibility to neuronal excitotoxicity (MGI Ref ID J:136582)
- infusion of high doses of capsaicin (5-10 um) into striatum results in dose-dependent excitotoxicity in neuronal cultures
- at doses sufficient to induce circling, no cell death is observed
- hearing/vestibular/ear phenotype
- circling (MGI Ref ID J:136582)
- infusion of capsaicin into striatum of results in stereotyped contralateral circling behavior within 5 minutes of delivery, while control mice without activation of Trpv1 show no effect
- homeostasis/metabolism phenotype
- increased susceptibility to neuronal excitotoxicity (MGI Ref ID J:136582)
- infusion of high doses of capsaicin (5-10 um) into striatum results in dose-dependent excitotoxicity in neuronal cultures
- at doses sufficient to induce circling, no cell death is observed
This mouse can be used to support research in many areas including:
Neurobiology Research
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Research Tools
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Fluorescent Proteins
Neurobiology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde | ||
|---|---|---|---|
| Allele Name | targeted insertion 1, Michael D Ehlers | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | ROSA-stop flox-TRPV1-IRES-ECFP; | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A targeting vector was used to insert a loxP-flanked neomycin cassette, rat Trpv1 (transient receptor potential cation channel, subfamily V, member 1) cDNA, and IRES-ECFP sequence into the Gt(ROSA)26Sor locus. [MGI Ref ID J:136582] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde, Standard PCR
Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Arenkiel BR; Klein ME; Davison IG; Katz LC; Ehlers MD. 2008. Genetic control of neuronal activity in mice conditionally expressing TRPV1. Nat Methods 5(4):299-302. [PubMed: 18327266] [MGI Ref ID J:136582]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375/MGL377
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, heterozygotes or homozygotes may be bred. Mating System Heterozygote x Heterozygote (Female x Male) 13-FEB-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Heterozygous for Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde
Pairs /Price (US dollars $) Pair Genotype $487.00 Heterozygous for Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde x Heterozygous for Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Heterozygous for Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde
Pairs /Price (US dollars $) Pair Genotype $633.10 Heterozygous for Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde x Heterozygous for Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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