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| Mice harboring the hCD2-iCre transgene have the human CD2 promoter and locus control region (LCR) directing expression of an optimized variant of Cre recombinase (iCre) to T cells and B cells (all B cell and T cell progenitors) and mice may be useful for generating conditional mutations in T cells and B cells. Because of the integration site of the transgene, the hCD2-iCre transgene is linked to the agouti coat color genes. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 25-JAN-13 Mating System Hemizygote x Noncarrier (Female x Male) 25-JAN-13 Species laboratory mouse Generation N16+F8 (23-MAR-09)
Generation DefinitionsDonating Investigator Dimitris Kioussis, MRC Natl Inst for Med Research Description
Mice hemizygous for this hCD2-iCre transgene are viable and fertile, with the human CD2 promoter and locus control region (LCR) directing expression of an optimized variant of Cre recombinase (iCre) to T cells and B cells (all committed B cell and T cell progenitors). Using crosses to a reporter strain, variegated germ line (testis) and a small monocyte-enriched population expression is also reported. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These hCD2-iCre transgenic mice may be useful for generating conditional mutations in T cells and B cells.Of note, this hCD2-iCre strain (Stock No. 008520) allows reliable deletion/gene targeting to be focused to T cells and B cells, whereas the Vav-iCre strain (Stock No. 008610) allows targeting throughout the entire hematopoietic compartment.
IMPORTANT NOTE: The donating investigator reports that while backcrossing these hCD2-iCre transgenic mice to C57BL/6 mice, transgenic mice always exhibited agouti coat color. This linkage suggests that the hCD2-iCre transgene integrated near agouti coat color genes.
Development
The hCD2-iCre transgene was designed with the human CD2 promoter, an optimized variant of Cre recombinase (iCre; improved with mammalian codon usage, removed putative cryptic splice sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals), an SV40 polyA signal, and the human CD2 locus control region (LCR). The transgene was microinjected into the pronuclei of fertilized oocytes from (CBA/Ca x C57BL/10) Fl mice. Founder mice were established (line ".4") and used to generate mutant mice. The resulting hCD2-iCre transgenic mice were subsequently bred to wildtype C57BL/6 mice for more than 15 generations prior to arrival at The Jackson Laboratory Repository. Upon arrival, the hCD2-iCre transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.IMPORTANT NOTE: The donating investigator reports that while backcrossing these hCD2-iCre transgenic mice to C57BL/6 mice, transgenic mice always exhibited agouti coat color. This linkage suggests that the hCD2-iCre transgene integrated near agouti coat color genes.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of CD2
View Strains carrying other alleles of CD2 (6 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Hematological Research
Immunology and Inflammation Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CD2-cre)4Kio | ||
|---|---|---|---|
| Allele Name | transgene insertion 4, Dimitris Kioussis | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | CD2-Cre; Tg(CD2-cre)1Kio; hCD2-Cre; hCD2-iCre; | ||
| Mutation Made By | Dimitris Kioussis, MRC Natl Inst for Med Research | ||
| Strain of Origin | (CBA/Ca x C57BL/10)F2 | ||
| Site of Expression | expressed in lymph nodes, thymus, spleen, Peyer's patches, intestinal cryptopatches and testes. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | CD2, CD2 molecule, human | ||
| Gene Symbol and Name | Tg(CD2-cre)4Kio, transgene insertion 4, Dimitris Kioussis | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(CD2-cre)1Kio; hCD2-Cre; hCD2-iCre; transgene insertion 1, Dimitris Kioussis; | ||
| Driver Note | CD2 | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a human CD2 promoter. The promoter was shown to be active in lymph nodes, thymus, spleen, Peyer's patches, intestinal cryptopatches and testes. [MGI Ref ID J:81568] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic iCre, Melt Curve Analysis
Generic iCre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
De Boer J; Williams A; Skavdis G; Harker N; Coles M; Tolaini M; Norton T; Williams K; Roderick K; Potocnik AJ; Kioussis D. 2003. Transgenic mice with hematopoietic and lymphoid specific expression of Cre. Eur J Immunol 33(2):314-25. [PubMed: 12548562] [MGI Ref ID J:81568]
Shimshek DR; Kim J; Hubner MR; Spergel DJ; Buchholz F; Casanova E; Stewart AF; Seeburg PH; Sprengel R. 2002. Codon-improved Cre recombinase (iCre) expression in the mouse. Genesis 32(1):19-26. [PubMed: 11835670] [MGI Ref ID J:78663]
Zhumabekov T; Corbella P; Tolaini M; Kioussis D. 1995. Improved version of a human CD2 minigene based vector for T cell-specific expression in transgenic mice. J Immunol Methods 185(1):133-40. [PubMed: 7665895] [MGI Ref ID J:105228]
Additional References
Tg(CD2-cre)4Kio relatedDumont C; Corsoni-Tadrzak A; Ruf S; de Boer J; Williams A; Turner M; Kioussis D; Tybulewicz VL. 2009. Rac GTPases play critical roles in early T-cell development. Blood 113(17):3990-8. [PubMed: 19088377] [MGI Ref ID J:148437]
Fedeli M; Napolitano A; Wong MP; Marcais A; de Lalla C; Colucci F; Merkenschlager M; Dellabona P; Casorati G. 2009. Dicer-dependent microRNA pathway controls invariant NKT cell development. J Immunol 183(4):2506-12. [PubMed: 19625646] [MGI Ref ID J:151790]
Finney BA; Schweighoffer E; Navarro-Nunez L; Benezech C; Barone F; Hughes CE; Langan SA; Lowe KL; Pollitt AY; Mourao-Sa D; Sheardown S; Nash GB; Smithers N; Reis e Sousa C; Tybulewicz VL; Watson SP. 2012. CLEC-2 and Syk in the megakaryocytic/platelet lineage are essential for development. Blood 119(7):1747-56. [PubMed: 22186994] [MGI Ref ID J:181715]
Gomez-del Arco P; Kashiwagi M; Jackson AF; Naito T; Zhang J; Liu F; Kee B; Vooijs M; Radtke F; Redondo JM; Georgopoulos K. 2010. Alternative promoter usage at the Notch1 locus supports ligand-independent signaling in T cell development and leukemogenesis. Immunity 33(5):685-98. [PubMed: 21093322] [MGI Ref ID J:167001]
Hodson DJ; Janas ML; Galloway A; Bell SE; Andrews S; Li CM; Pannell R; Siebel CW; MacDonald HR; De Keersmaecker K; Ferrando AA; Grutz G; Turner M. 2010. Deletion of the RNA-binding proteins ZFP36L1 and ZFP36L2 leads to perturbed thymic development and T lymphoblastic leukemia. Nat Immunol 11(8):717-24. [PubMed: 20622884] [MGI Ref ID J:162388]
Hooper J; Maurice D; Argent-Katwala MJ; Weston K. 2008. Myb proteins regulate expression of histone variant H2A.Z during thymocyte development. Immunology 123(2):282-9. [PubMed: 17931383] [MGI Ref ID J:134915]
Karamitros D; Kotantaki P; Lygerou Z; Veiga-Fernandes H; Pachnis V; Kioussis D; Taraviras S. 2010. Differential geminin requirement for proliferation of thymocytes and mature T cells. J Immunol 184(5):2432-41. [PubMed: 20107189] [MGI Ref ID J:159657]
Li S; Symonds AL; Zhu B; Liu M; Raymond MV; Miao T; Wang P. 2011. Early growth response gene-2 (egr-2) regulates the development of B and T cells. PLoS One 6(4):e18498. [PubMed: 21533228] [MGI Ref ID J:172386]
Lu L; Wang J; Zhang F; Chai Y; Brand D; Wang X; Horwitz DA; Shi W; Zheng SG. 2010. Role of SMAD and non-SMAD signals in the development of Th17 and regulatory T cells. J Immunol 184(8):4295-306. [PubMed: 20304828] [MGI Ref ID J:159864]
Menzel U; Kosteas T; Tolaini M; Killeen N; Roderick K; Kioussis D. 2011. Modulation of the murine CD8 gene complex following the targeted integration of human CD2-locus control region sequences. J Immunol 187(7):3712-20. [PubMed: 21880987] [MGI Ref ID J:179326]
Miao T; Raymond M; Bhullar P; Ghaffari E; Symonds AL; Meier UC; Giovannoni G; Li S; Wang P. 2013. Early Growth Response Gene-2 Controls IL-17 Expression and Th17 Differentiation by Negatively Regulating Batf. J Immunol 190(1):58-65. [PubMed: 23203924] [MGI Ref ID J:190832]
Mylona A; Nicolas R; Maurice D; Sargent M; Tuil D; Daegelen D; Treisman R; Costello P. 2011. The essential function for serum response factor in T-cell development reflects its specific coupling to extracellular signal-regulated kinase signaling. Mol Cell Biol 31(2):267-76. [PubMed: 21098124] [MGI Ref ID J:169423]
Outram SV; Hager-Theodorides AL; Shah DK; Rowbotham NJ; Drakopoulou E; Ross SE; Lanske B; Dessens JT; Crompton T. 2009. Indian hedgehog (Ihh) both promotes and restricts thymocyte differentiation. Blood 113(10):2217-28. [PubMed: 19109233] [MGI Ref ID J:145997]
Ramadani F; Bolland DJ; Garcon F; Emery JL; Vanhaesebroeck B; Corcoran AE; Okkenhaug K. 2010. The PI3K isoforms p110alpha and p110delta are essential for pre-B cell receptor signaling and B cell development. Sci Signal 3(134):ra60. [PubMed: 20699475] [MGI Ref ID J:185404]
Rongvaux A; Galli M; Denanglaire S; Van Gool F; Dreze PL; Szpirer C; Bureau F; Andris F; Leo O. 2008. Nicotinamide phosphoribosyl transferase/pre-B cell colony-enhancing factor/visfatin is required for lymphocyte development and cellular resistance to genotoxic stress. J Immunol 181(7):4685-95. [PubMed: 18802071] [MGI Ref ID J:141448]
Saunders A; Webb LM; Janas ML; Hutchings A; Pascall J; Carter C; Pugh N; Morgan G; Turner M; Butcher GW. 2010. Putative GTPase GIMAP1 is critical for the development of mature B and T lymphocytes. Blood 115(16):3249-57. [PubMed: 20194894] [MGI Ref ID J:160782]
Schindler JW; Van Buren D; Foudi A; Krejci O; Qin J; Orkin SH; Hock H. 2009. TEL-AML1 corrupts hematopoietic stem cells to persist in the bone marrow and initiate leukemia. Cell Stem Cell 5(1):43-53. [PubMed: 19570513] [MGI Ref ID J:151639]
Shi J; Petrie HT. 2012. Activation kinetics and off-target effects of thymus-initiated cre transgenes. PLoS One 7(10):e46590. [PubMed: 23049709] [MGI Ref ID J:191954]
Sullivan RP; Leong JW; Schneider SE; Keppel CR; Germino E; French AR; Fehniger TA. 2012. MicroRNA-deficient NK cells exhibit decreased survival but enhanced function. J Immunol 188(7):3019-30. [PubMed: 22379033] [MGI Ref ID J:183097]
Xiao C; Srinivasan L; Calado DP; Patterson HC; Zhang B; Wang J; Henderson JM; Kutok JL; Rajewsky K. 2008. Lymphoproliferative disease and autoimmunity in mice with increased miR-17-92 expression in lymphocytes. Nat Immunol 9(4):405-14. [PubMed: 18327259] [MGI Ref ID J:133215]
Zhu B; Symonds AL; Martin JE; Kioussis D; Wraith DC; Li S; Wang P. 2008. Early growth response gene 2 (Egr-2) controls the self-tolerance of T cells and prevents the development of lupuslike autoimmune disease. J Exp Med 205(10):2295-307. [PubMed: 18779345] [MGI Ref ID J:141136]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375
Colony Maintenance
Breeding & Husbandry Because of the integration site of the transgene, hCD2-iCre transgenic mice have agouti coat color. When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to C57BL/6J. The donating investigator reports that homozygous mice are not viable. Mating System Noncarrier x Hemizygote (Female x Male) 25-JAN-13 Hemizygote x Noncarrier (Female x Male) 25-JAN-13 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(CD2-cre)4Kio
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(CD2-cre)4Kio x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(CD2-cre)4Kio Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(CD2-cre)4Kio
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(CD2-cre)4Kio x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(CD2-cre)4Kio Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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