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| These transgenic mice express the mouse apolipoprotein E gene, Apoe, under the control of the mouse Cyp21a1, cytochrome P450, family 21, subfamily a, polypeptide 1, promoter, specific to the adrenal gland. This mutant mouse strain may be useful in studies of atherosclerosis and hypercholesterolemia. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N10+N1pN1
Generation DefinitionsDonating Investigator Craig Malbon, Stony Brook University Description
These transgenic mice express the mouse apolipoprotein E gene, Apoe, under the control of the mouse Cyp21a1, cytochrome P450, family 21, subfamily a, polypeptide 1, promoter. Transgene expression is specific to the adrenal gland. When crossed to B6.129P2-Apoetm1Unc/J (Stock No. 002052), transgene mRNA is not detected in the liver, testes or peritoneal macrophages. Founder line 619 mice, hemizygous for the transgene and homozygous for the Apoetm1Unc allele, express 1.6 pg Apoe mRNA per mg total RNA in the adrenal gland. This transgenic strain carries only the transgene. This mutant mouse strain may be useful in studies of atherosclerosis and hypercholesterolemia.Development
A transgenic construct containing the mouse apolipoprotein E gene under the control of the mouse Cyp21a1, cytochrome P450, family 21, subfamily a, polypeptide 1, promoter was injected into fertilized FVB/N mouse eggs. Founder line 619 animals were bred to homozygous B6;129-Apoe tm1Unc mice for 2 generations. The double mutant mice were then crossed to B6.129P2-Apoe tm1Unc/J (Stock No. 002052) for nine generations. This transgenic strain was generated by selectively breeding for the transgene from the double mutant strain.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Apoe
014556 129S6/SvEv-Apoetm4Mae/J 007069 AKR.129P2(B6)-Apoetm1Unc/J 002246 B6.129-Apoetm1Unc Ldlrtm1Her/J 002052 B6.129P2-Apoetm1Unc/J 013040 B6.Cg-Apoetm1Unc Ins2Akita/J 007224 B6.Cg-Apoetm1Unc (D12Mit182-D12Mit2)/Pgn 004632 B6.Cg-Tg(GFAP-APOE_i2)14Hol Apoetm1Unc/J 004633 B6.Cg-Tg(GFAP-APOE_i3)37Hol Apoetm1Unc/J 004631 B6.Cg-Tg(GFAP-APOE_i4)1Hol Apoetm1Unc/J 002878 B6;129-Apobtm1Sgy Apoetm1Unc/J 002879 B6;129-Apobtm2Sgy Apoetm1Unc/J 002245 B6;129-Apoetm1Unc Ldlrtm1Her/J 004362 B6;129-Scarb1tm1Kri Apoetm1Unc/J 007067 D2.129P2(B6)-Apoetm1Unc/J 013719 D2.Cg-Apoetm1Unc Ins2Akita/J View Strains carrying other alleles of Apoe (15 strains)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Cyp21a1-Apoe)619Fet/0
involves: 129P2/OlaHsd * C57BL/6 * FVB/N
- homeostasis/metabolism phenotype
- increased circulating cholesterol level
- total plasma cholesterol levels (TPC) are about 646 mg/dl (mean TPC approx 662 mg/dl) (MGI Ref ID J:130658)
This mouse can be used to support research in many areas including:
Cardiovascular Research
Atherosclerosis
Hypercholesterolemia
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Cyp21a1-Apoe)619Fet | ||
|---|---|---|---|
| Allele Name | transgene insertion 619, Fayanne Thorngate | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Line 619; | ||
| Mutation Made By | Fayanne Thorngate, Stony Brook University | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | Apoe, apolipoprotein E, mouse, laboratory | ||
| Promoter | Cyp21a1, cytochrome P450, family 21, subfamily a, polypeptide 1, mouse, laboratory | ||
| Molecular Note | A construct containing approximately 6 kb of the mouse Cyp21a1 promoter attached to the mouse Apoe gene was used for injection into fertilized FVB/N oocytes. Ten independent founder lines were generated. Line 619 was donated to the Jackson Laboratory andis representative of low expressing Apoe lines (lines 619 and 620). Only adrenal expression of Apoe was detected for these lines with Line 619 having the lowest mRNA expression (1.6 pg Apoe mRNA per microgram of total RNA). Mice have only 1-2% of wild-type plasma Apoe protein level. [MGI Ref ID J:130658] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Apoetm1Unc, High Resolution Melting
Apoetm1Unc, Standard PCR
Tg(Cyp21a1-Apoe), Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Thorngate FE; Rudel LL; Walzem RL; Williams DL. 2000. Low levels of extrahepatic nonmacrophage ApoE inhibit atherosclerosis without correcting hypercholesterolemia in ApoE-deficient mice. Arterioscler Thromb Vasc Biol 20(8):1939-45. [PubMed: 10938015] [MGI Ref ID J:130658]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgene carrier mice may be bred with wildtype mice from the colony or with C57BL/6J inbred mice.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiriesContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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