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| These transgenic mice express the mouse apolipoprotein E gene, Apoe, under the control of the mouse Cyp21a1, cytochrome P450, family 21, subfamily a, polypeptide 1, promoter, specific to the adrenal gland. This mutant mouse strain may be useful in studies of atherosclerosis and hypercholesterolemia. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 30-DEC-08 Species laboratory mouse Generation N10+N2 (21-APR-09) Donating Investigator Craig Malbon, Stony Brook University Description
These transgenic mice express the mouse apolipoprotein E gene, Apoe, under the control of the mouse Cyp21a1, cytochrome P450, family 21, subfamily a, polypeptide 1, promoter. Transgene expression is specific to the adrenal gland. When crossed to B6.129P2-Apoetm1Unc/J (Stock No. 002052), transgene mRNA is not detected in the liver, testes or peritoneal macrophages. Founder line 619 mice, hemizygous for the transgene and homozygous for the Apoetm1Unc allele, express 1.6 pg Apoe mRNA per mg total RNA in the adrenal gland. This transgenic strain carries only the transgene. This mutant mouse strain may be useful in studies of atherosclerosis and hypercholesterolemia.Development
A transgenic construct containing the mouse apolipoprotein E gene under the control of the mouse Cyp21a1, cytochrome P450, family 21, subfamily a, polypeptide 1, promoter was injected into fertilized FVB/N mouse eggs. Founder line 619 animals were bred to homozygous B6;129-Apoe tm1Unc mice for 2 generations. The double mutant mice were then crossed to B6.129P2-Apoe tm1Unc/J (Stock No. 002052) for nine generations. This transgenic strain was generated by selectively breeding for the transgene from the double mutant strain.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Apoe
007069 AKR.129P2(B6)-Apoetm1Unc/J 002246 B6.129-Apoetm1Unc Ldlrtm1Her/J 002052 B6.129P2-Apoetm1Unc/J 007224 B6.Cg-Apoetm1Unc (D12Mit182-D12Mit2)/Pgn 004632 B6.Cg-Tg(GFAP-APOE*2)14Hol Apoetm1Unc/J 004633 B6.Cg-Tg(GFAP-APOE*3)37Hol Apoetm1Unc/J 004631 B6.Cg-Tg(GFAP-APOE*4)1Hol Apoetm1Unc/J 002878 B6;129-Apobtm1Sgy Apoetm1Unc/J 002879 B6;129-Apobtm2Sgy Apoetm1Unc/J 002245 B6;129-Apoetm1Unc Ldlrtm1Her/J 004362 B6;129-Scarb1tm1Kri Apoetm1Unc/J 007067 D2.129P2(B6)-Apoetm1Unc/J View Strains carrying other alleles of Apoe (12 strains)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Cyp21a1-Apoe)619Fet/0
involves: 129P2/OlaHsd * C57BL/6 * FVB/N
- homeostasis/metabolism phenotype
- increased circulating cholesterol level (MGI Ref ID J:130658)
- total plasma cholesterol levels (TPC) are about 646 mg/dl (mean TPC approx 662 mg/dl)
This mouse can be used to support research in many areas including:
Cardiovascular Research
Atherosclerosis
Hypercholesterolemia
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Cyp21a1-Apoe)619Fet | ||
|---|---|---|---|
| Allele Name | transgene insertion 619, Fayanne Thorngate | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Line 619; | ||
| Mutation Made By | Fayanne Thorngate, Stony Brook University | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | Apoe, apolipoprotein E, mouse, laboratory | ||
| Promoter | Cyp21a1, cytochrome P450, family 21, subfamily a, polypeptide 1, mouse, laboratory | ||
| Molecular Note | A construct containing approximately 6 kb of the mouse Cyp21a1 promoter attached to the mouse Apoe gene was used for injection into fertilized FVB/N oocytes. Ten independent founder lines were generated. Line 619 was donated to the Jackson Laboratory andis representative of low expressing Apoe lines (lines 619 and 620). Only adrenal expression of Apoe was detected for these lines with Line 619 having the lowest mRNA expression (1.6 pg Apoe mRNA per microgram of total RNA). Mice have only 1-2% of wild-type plasma Apoe protein level. [MGI Ref ID J:130658] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Apoetm1Unc, Standard PCR
Tg(Cyp21a1-Apoe), Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Thorngate FE; Rudel LL; Walzem RL; Williams DL. 2000. Low levels of extrahepatic nonmacrophage ApoE inhibit atherosclerosis without correcting hypercholesterolemia in ApoE-deficient mice. Arterioscler Thromb Vasc Biol 20(8):1939-45. [PubMed: 10938015] [MGI Ref ID J:130658]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes. Mating System Noncarrier x Hemizygote (Female x Male) 30-DEC-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Cyp21a1-Apoe)619Fet
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Cyp21a1-Apoe)619Fet x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Cyp21a1-Apoe)619Fet
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Cyp21a1-Apoe)619Fet
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Cyp21a1-Apoe)619Fet x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Cyp21a1-Apoe)619Fet
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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