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| When these Ngn1-CreERT2 transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Neurog1 expressing cells such as neurons in the cortex, hippocampus, thalamus, hypothalamus and the cochlear-vestibular ganglion. This mutant mouse strain may be useful for studying neuron development and lineage mapping of Neurog1 expressing cells. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 23-JAN-09 Species laboratory mouse Generation N5F?+ (22-JAN-09) Donating Investigator Lisa Goodrich, Harvard Medical School Description
These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse neurogenin 1, Neurog1, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in the cortex, hippocampus, thalamus, hypothalamus and cochlear-vestibular ganglion. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are not viable. This mutant mouse strain may be useful for studying neuron development and lineage mapping of Neurog1 expressing cells.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A targeting construct containing an FRT site flanked PGK-Neo cassette and sequence encoding cre/ESR1 (CreERT2) was inserted into the single coding exon of the gene in a BAC. The PGK-neo cassette was removed by transient Flp-recombinase expression. The resulting BAC transgene was microinjected into C57BL/6 donor eggs. Founder line 7 was subsequently established. The mice were then crossed to B6129PF1/J for five generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of ESR1
006661 129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J 007179 129S.Cg-Tg(UBC-cre/ESR1)1Ejb/J 005975 B6.Cg-Tg(Plp1-cre/ESR1)3Pop/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 008085 B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J 009614 B6.Cg-Tg(Wfs1-cre/ERT2)2Aibs/J 007001 B6;129S-Tg(UBC-cre/ESR1)1Ejb/J 009103 B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 004995 C3H-Tg(Camk2a-Creb1/ESR1)3Sva/J 002712 FVB/N-Tg(PF4MER)6Kra/J 007684 STOCK Tg(Atoh1-cre/ESR1)14Fsh/J 008861 STOCK Tg(Ela1-Cre/ESR1)1Stof/J View Strains carrying other alleles of ESR1 (13 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression: Inducible
Developmental Biology Research
Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Neurog1-cre/ESR1)1Good | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Lisa V Goodrich | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Ngn1-CreERT2; | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | Cre is expressed in neurons in the cortex, hippocampus, thalamus, hypothalamus and the cochlear-vestibular ganglion. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | ESR1, estrogen receptor 1, human | ||
| Promoter | Neurog1, neurogenin 1, mouse, laboratory | ||
| Driver Note | Neurog1 | ||
| Inducible Note | induced by tamoxifen | ||
| Molecular Note | A transgene was constructed where cre recombinase fused to the ligand binding domain of a mutant human estrogen receptor was placed under the control of the upstream promoter and the downstream untranslated region of the Neurog1 gene. Cre activity was detected in cochlear ganglion neurons of transgenic mice when crossed with a cre-reporter strain and dosing with tamoxifen. [MGI Ref ID J:130813] | ||
| Gene Symbol and Name | Tg(Neurog1-cre/ESR1)1Good, transgene insertion 1, Lisa V Goodrich | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Ngn1-CreERT2; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Neurog1-cre/ESR1)1Good, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Koundakjian EJ; Appler JL; Goodrich LV. 2007. Auditory neurons make stereotyped wiring decisions before maturation of their targets. J Neurosci 27(51):14078-88. [PubMed: 18094247] [MGI Ref ID J:130813]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375/MGL377
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator reports that homozygotes are not viable. Mating System Noncarrier x Hemizygote (Female x Male) 23-JAN-09
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Neurog1-cre/ESR1)1Good
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Neurog1-cre/ESR1)1Good x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Neurog1-cre/ESR1)1Good
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Neurog1-cre/ESR1)1Good
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Neurog1-cre/ESR1)1Good x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Neurog1-cre/ESR1)1Good
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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