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Common Names: Pf4-Cre;    
These transgenic mice express a codon-improved Cre recombinase (iCre) under the control of the mouse Pf4 (platelet factor 4), or Cxcl4, promoter. Cre recombinase expression is detected in the majority of megakaryocytes. This strain represents an effective tool for generating megakaryocyte lineage-restricted specific-targeted mutants.


Strain Information

Former Names C57BL/6-Tg(Cxcl4-cre)Q3Rsko/J    (Changed: 05-MAR-09 )
Type Coisogenic; Transgenic;
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Mating SystemNoncarrier x Hemizygote         (Female x Male)   15-JAN-09
Specieslaboratory mouse
GenerationF?+F15 (05-MAY-15)
Generation Definitions
Donating Investigator Radek Skoda,   University Hospital Basel

These transgenic mice express a codon-improved Cre recombinase (iCre) under the control of the mouse Pf4 (platelet factor 4), or Cxcl4, promoter. Cre recombinase expression is detected in the majority of megakaryocytes. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The BAC clone used to generate this strain also contains 4 other genes: Cxcl5, Cxcl7, Cxcl15 and Cxcl3. The additional copy of these chemokine genes in the BAC has no effect on blood count of the mutant mice. This strain represents an effective tool for generating megakaryocyte lineage-restricted specific-targeted mutants.

A 100kb BAC containing the mouse Pf4 (platelet factor 4), or Cxcl4, gene with exon 1 replaced by a construct containing the mouse Pf4 (platelet factor 4), or Cxcl4, promoter, a codon-improved Cre recombinase (iCre) sequence, bovine growth hormone polyadenylation signal, nuclear localization signal and a myc tag was injected into fertilized C57BL/6 fertilized oocytes. Founder line Q3 was established, carrying 1 copy of the transgene. Founder animals were maintained as hemizygotes on the C57BL/6 background.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Information

   000664 C57BL/6J
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Pf4
007243   B6;SJL-Tg(Cxcl4-F5)1Dgi/J
View Strains carrying other alleles of Pf4     (1 strain)

Strains carrying other alleles of cre
004337   129(Cg)-Foxg1tm1(cre)Skm/J
008569   129-Alpltm1(cre)Nagy/J
005989   129;FVB-Tg(PTH-cre)4167Slib/J
007179   129S.Cg-Tg(UBC-cre/ERT2)1Ejb/J
007915   129S.FVB-Tg(Amh-cre)8815Reb/J
003328   129S/Sv-Tg(Prm-cre)58Og/J
026200   129S1.Cg-Tg(Vsx2-cre)2690Chow/J
004302   129S1/Sv-Hprttm1(cre)Mnn/J
022137   129S4.Cg-Tg(Wnt1-cre)2Sor/J
003960   129S6-Tg(Prnp-GFP/cre)1Blw/J
008523   129S6.Cg-Tg(NPHS2-cre)295Lbh/BroJ
009587   B6(129S4)-Et(icre)1402Rdav/J
009588   B6(129S4)-Et(icre)1470Rdav/J
009589   B6(129S4)-Et(icre)1555Rdav/J
009586   B6(129S4)-Et(icre)754Rdav/J
012687   B6(129S4)-Tg(SYN1-icre/mRFP1)9934Rdav/J
010774   B6(Cg)-Calb2tm1(cre)Zjh/J
017562   B6(Cg)-Cd8atm1.1(cre)Koni/J
012704   B6(Cg)-Crhtm1(cre)Zjh/J
018448   B6(Cg)-Foxn1tm3(cre)Nrm/J
026801   B6(Cg)-Ins1tm1.1(cre)Thor/J
016223   B6(Cg)-Tg(Phox2b-cre)3Jke/J
016959   B6.129(Cg)-Foxp3tm4(YFP/cre)Ayr/J
023055   B6.129(Cg)-Krt12tm3(cre)Wwk/J
008320   B6.129-Leprtm2(cre)Rck/J
017526   B6.129-Nos1tm1(cre)Mgmj/J
005697   B6.129-Otx1tm4(cre)Asim/J
018938   B6.129-Tac2tm1.1(cre)Qima/J
017769   B6.129-Trpv1tm1(cre)Bbm/J
004146   B6.129-Tg(Pcp2-cre)2Mpin/J
008710   B6.129P2(129S4)-Hprttm10(Ple162-EGFP/cre)Ems/Mmjax
008877   B6.129P2(129S4)-Hprttm12(Ple177-EGFP/cre)Ems/Mmjax
009116   B6.129P2(129S4)-Hprttm16(Ple167-EGFP/cre)Ems/Mmjax
008709   B6.129P2(129S4)-Hprttm9(Ple178-EGFP/cre)Ems/Mmjax
006785   B6.129P2(C)-Cd19tm1(cre)Cgn/J
006084   B6.129P2(Cg)-Foxg1tm1(cre)Skm/J
010611   B6.129P2(Cg)-Ighg1tm1(IRES-cre)Cgn/J
007770   B6.129P2-Aicdatm1(cre)Mnz/J
004781   B6.129P2-Lyz2tm1(cre)Ifo/J
017320   B6.129P2-Pvalbtm1(cre)Arbr/J
017915   B6.129S(Cg)-Pgrtm1.1(cre)Shah/AndJ
013594   B6.129S-Atoh1tm5.1(Cre/PGR)Hzo/J
021794   B6.129S1(Cg)-Ascl3tm1.1(EGFP/cre)Ovi/J
024637   B6.129S1(SJL)-Nkx2-5tm2(cre)Rph/J
006600   B6.129S1-Mnx1tm4(cre)Tmj/J
005628   B6.129S2-Emx1tm1(cre)Krj/J
022510   B6.129S4-Gpr88tm1.1(cre/GFP)Rpa/J
017578   B6.129S4-Mcpt8tm1(cre)Lky/J
003755   B6.129S4-Meox2tm1(cre)Sor/J
007893   B6.129S4-Myf5tm3(cre)Sor/J
006878   B6.129S6-Taglntm2(cre)Yec/J
012839   B6.129X1(Cg)-Tnfrsf4tm2(cre)Nik/J
008712   B6.129X1-Twist2tm1.1(cre)Dor/J
006054   B6.C-Tg(CMV-cre)1Cgn/J
020811   B6.C-Tg(Pgk1-cre)1Lni/CrsJ
023530   B6.Cg-Avptm1.1(cre)Hze/J
013590   B6.Cg-Braftm1Mmcm Ptentm1Hwu Tg(Tyr-cre/ERT2)13Bos/BosJ
006230   B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J
012358   B6.Cg-Pvalbtm1.1(cre)Aibs/J
005622   B6.Cg-Shhtm1(EGFP/cre)Cjt/J
022762   B6.Cg-Zfp335tm1.2Caw Emx1tm1(cre)Krj/J
017346   B6.Cg-Tg(A930038C07Rik-cre)1Aibs/J
006149   B6.Cg-Tg(ACTA1-cre)79Jme/J
003574   B6.Cg-Tg(Alb-cre)21Mgn/J
006881   B6.Cg-Tg(Aqp2-cre)1Dek/J
011104   B6.Cg-Tg(Atoh1-cre)1Bfri/J
008520   B6.Cg-Tg(CD2-cre)4Kio/J
009350   B6.Cg-Tg(CDX2-cre)101Erf/J
009352   B6.Cg-Tg(CDX2-cre*)189Erf/J
027310   B6.Cg-Tg(Camk2a-cre)2Szi/J
027400   B6.Cg-Tg(Camk2a-cre)3Szi/J
005359   B6.Cg-Tg(Camk2a-cre)T29-1Stl/J
022071   B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ
012237   B6.Cg-Tg(Cdh16-cre)91Igr/J
006368   B6.Cg-Tg(Cr2-cre)3Cgn/J
006663   B6.Cg-Tg(Eno2-cre)39Jme/J
005069   B6.Cg-Tg(Fabp4-cre)1Rev/J
012712   B6.Cg-Tg(Fev-cre)1Esd/J
012849   B6.Cg-Tg(GFAP-cre/ERT2)505Fmv/J
012886   B6.Cg-Tg(Gfap-cre)73.12Mvs/J
024098   B6.Cg-Tg(Gfap-cre)77.6Mvs/2J
009642   B6.Cg-Tg(Gh1-cre)1Sac/J
024474   B6.Cg-Tg(Il9-cre)#Stck/J
003573   B6.Cg-Tg(Ins2-cre)25Mgn/J
008068   B6.Cg-Tg(Itgax-cre)1-1Reiz/J
008781   B6.Cg-Tg(Kap-cre)29066/2Sig/J
003802   B6.Cg-Tg(Lck-cre)548Jxm/J
006889   B6.Cg-Tg(Lck-cre)I540Jxm/J
012837   B6.Cg-Tg(Lck-icre)3779Nik/J
009643   B6.Cg-Tg(Lhb-cre)1Sac/J
008330   B6.Cg-Tg(Mc4r-cre)25Rck/J
003556   B6.Cg-Tg(Mx1-cre)1Cgn/J
007742   B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
008205   B6.Cg-Tg(NPHS2-cre)295Lbh/J
003771   B6.Cg-Tg(Nes-cre)1Kln/J
010536   B6.Cg-Tg(Pcp2-cre)3555Jdhu/J
005975   B6.Cg-Tg(Plp1-cre/ERT)3Pop/J
008827   B6.Cg-Tg(Prdm1-cre)1Masu/J
005584   B6.Cg-Tg(Prrx1-cre)1Cjt/J
003967   B6.Cg-Tg(Rbp3-cre)528Jxm/J
021614   B6.Cg-Tg(S100A8-cre,-EGFP)1Ilw/J
008454   B6.Cg-Tg(Sox2-cre)1Amc/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
003966   B6.Cg-Tg(Syn1-cre)671Jxm/J
017491   B6.Cg-Tg(Tagln-cre)1Her/J
004128   B6.Cg-Tg(Tek-cre)12Flv/J
008863   B6.Cg-Tg(Tek-cre)1Ywa/J
008601   B6.Cg-Tg(Th-cre)1Tmd/J
012328   B6.Cg-Tg(Tyr-cre/ERT2)13Bos/J
008085   B6.Cg-Tg(UBC-cre/ERT2)1Ejb/J
008610   B6.Cg-Tg(Vav1-cre)A2Kio/J
004586   B6.Cg-Tg(Vil-cre)997Gum/J
021504   B6.Cg-Tg(Vil1-cre)1000Gum/J
008735   B6.Cg-Tg(Wap-cre)11738Mam/JKnwJ
009614   B6.Cg-Tg(Wfs1-cre/ERT2)2Aibs/J
009107   B6.Cg-Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J
022501   B6.Cg-Tg(Wnt1-cre)2Sor/J
006234   B6.Cg-Tg(tetO-cre)1Jaw/J
016832   B6.FVB(129)-Tg(Alb1-cre)1Dlr/J
005657   B6.FVB(129)-Tg(Myh6-cre/Esr1*)1Jmk/J
024688   B6.FVB(129S)-Tg(Pax6-GFP/cre)1Rilm/J
006475   B6.FVB(129S4)-Tg(Ckmm-cre)5Khn/J
006451   B6.FVB(129X1)-Tg(Sim1-cre)1Lowl/J
006333   B6.FVB(Cg)-Tg(Neurog3-cre)C1Able/J
014643   B6.FVB-Tg(CMA1-cre)6Thhe/J
006137   B6.FVB-Tg(Cdh5-cre)7Mlia/J
018980   B6.FVB-Tg(Ddx4-cre)1Dcas/KnwJ
003724   B6.FVB-Tg(EIIa-cre)C5379Lmgd/J
011069   B6.FVB-Tg(Gh1-cre)bKnmn/J
011038   B6.FVB-Tg(Myh6-cre)2182Mds/J
014647   B6.FVB-Tg(Pdx1-cre)6Tuv/J
010714   B6.FVB-Tg(Pomc-cre)1Lowl/J
022791   B6.FVB-Tg(Rorc-cre)1Litt/J
017535   B6.FVB-Tg(Slc32a1-cre)2.1Hzo/FrkJ
017490   B6.FVB-Tg(Stra8-icre)1Reb/LguJ
024670   B6.FVB-Tg(Ucp1-cre)1Evdr/J
003394   B6.FVB-Tg(Zp3-cre)3Mrt/J
006660   B6.SJL-Slc6a3tm1.1(cre)Bkmn/J
003552   B6129-Tg(Wap-cre)11738Mam/J
023161   B6129S-Tg(Foxp3-EGFP/cre)1aJbs/J
021025   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-cre)Haho/J
010529   B6;129-Myf5tm1(cre)Mrc/J
010528   B6;129-Myf6tm2(cre)Mrc/J
017525   B6;129-Ntstm1(cre)Mgmj/J
005549   B6;129-Pax3tm1(cre)Joe/J
017968   B6;129-Tg(Cdh5-cre)1Spe/J
024860   B6;129-Tg(Drd1-cre)120Mxu/Mmjax
010988   B6;129P-Cyp11a1tm1(GFP/cre)Pzg/J
008529   B6;129P-Tg(Neurog1-cre/ERT2)1Good/J
012601   B6;129P2-Lyve1tm1.1(EGFP/cre)Cys/J
006668   B6;129P2-Omptm4(cre)Mom/MomJ
008069   B6;129P2-Pvalbtm1(cre)Arbr/J
012373   B6;129S-Hoxb1tm1(cre)Og/J
024234   B6;129S-Oxttm1.1(cre)Dolsn/J
023526   B6;129S-Rorbtm1.1(cre)Hze/J
023527   B6;129S-Slc17a7tm1.1(cre)Hze/J
023525   B6;129S-Snap25tm2.1(cre)Hze/J
021877   B6;129S-Tac1tm1.1(cre)Hze/J
021878   B6;129S-Tac2tm1.1(cre)Hze/J
017685   B6;129S-Wisp3tm1(cre)Mawa/J
007001   B6;129S-Tg(UBC-cre/ERT2)1Ejb/J
009388   B6;129S1-Osr2tm2(cre)Jian/J
012463   B6;129S4-Foxd1tm1(GFP/cre)Amc/J
011105   B6;129S4-Olig1tm1(cre)Rth/J
006410   B6;129S6-Chattm2(cre)Lowl/J
009616   B6;C3-Tg(A930038C07Rik-cre)4Aibs/J
012433   B6;C3-Tg(ACTA1-rtTA,tetO-cre)102Monk/J
008844   B6;C3-Tg(Ctgf-cre)2Aibs/J
008839   B6;C3-Tg(Cyp39a1-cre)1Aibs/J
009117   B6;C3-Tg(Cyp39a1-cre)7Aibs/J
008848   B6;C3-Tg(Mybpc1-cre)2Aibs/J
009111   B6;C3-Tg(Scnn1a-cre)1Aibs/J
009112   B6;C3-Tg(Scnn1a-cre)2Aibs/J
009613   B6;C3-Tg(Scnn1a-cre)3Aibs/J
009103   B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J
025806   B6;CBA-Tg(Gsx2-cre)1Kess/J
026555   B6;CBA-Tg(Lhx6-cre)1Kess/J
025807   B6;CBA-Tg(Sox10-cre)1Wdr/J
024507   B6;CBA-Tg(Tbx21-cre)1Dlc/J
017494   B6;D-Tg(Tshz3-GFP/cre)43Amc/J
024926   B6;D2-Tg(Fshr-cre)1Ldu/J
003466   B6;D2-Tg(Sycp1-cre)4Min/J
014160   B6;DBA-Tg(S100b-EGFP/cre/ERT2)22Amc/J
014159   B6;DBA-Tg(Tmem100-EGFP/cre/ERT2)30Amc/J
015855   B6;DBA-Tg(Upk3a-GFP/cre/ERT2)26Amc/J
010803   B6;FVB-Tg(Adipoq-cre)1Evdr/J
018422   B6;FVB-Tg(Aicda-cre)1Rcas/J
023748   B6;FVB-Tg(Aldh1l1-cre)JD1884Gsat/J
011087   B6;FVB-Tg(Crh-cre)1Kres/J
008533   B6;FVB-Tg(Cspg4-cre)1Akik/J
003734   B6;FVB-Tg(GZMB-cre)1Jcb/J
015850   B6;SJL-Pde6b+ Tg(Rho-icre)1Ck/Boc
004426   B6;SJL-Tg(Cga-cre)3Sac/J
003554   B6;SJL-Tg(Col2a1-cre)1Bhr/J
017738   B6;SJL-Tg(Foxl1-cre)1Khk/J
005249   B6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J
019893   B6;SJL-Tg(Tex101-icre)2Lzj/J
007252   B6Ei.129S4-Tg(Prm-cre)58Og/EiJ
025524   B6J.B6N(Cg)-Cx3cr1tm1.1(cre)Jung/J
018956   B6N.129P2(B6)-Lyz2tm1(cre)Ifo/J
018958   B6N.129P2-Cd19tm1(cre)Cgn/J
021077   B6N.129S1-Mrgprb4tm3(cre)And/J
018957   B6N.129S6(B6)-Chattm2(cre)Lowl/J
017911   B6N.129S6(Cg)-Esr1tm1.1(cre)And/J
019013   B6N.129S6(Cg)-Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
018974   B6N.B6-Tg(Nr4a1-EGFP/cre)820Khog/J
019021   B6N.Cg-Ccktm1.1(cre)Zjh/J
019022   B6N.Cg-Gad2tm2(cre)Zjh/J
018973   B6N.Cg-Ssttm2.1(cre)Zjh/J
018961   B6N.Cg-Tg(Alb-cre)21Mgn/J
018966   B6N.Cg-Tg(Camk2a-cre)T29-1Stl/J
018965   B6N.Cg-Tg(Fabp4-cre)1Rev/J
017310   B6N.Cg-Tg(Hsd17b1-icre/ERT2)3Casa/J
018960   B6N.Cg-Tg(Ins2-cre)25Mgn/J
018967   B6N.Cg-Tg(Itgax-cre)1-1Reiz/J
018964   B6N.Cg-Tg(KRT14-cre)1Amc/J
019103   B6N.Cg-Tg(Nes-cre)1Kln/CjDswJ
014094   B6N.Cg-Tg(Sox2-cre)1Amc/J
018968   B6N.Cg-Tg(Vav1-cre)A2Kio/J
018963   B6N.Cg-Tg(Vil-cre)997Gum/J
018972   B6N.FVB(B6)-Tg(Myh6-cre)2182Mds/J
019099   B6N.FVB-Tg(ACTB-cre)2Mrt/CjDswJ
019509   B6N.FVB-Tg(BGLAP-cre)1Clem/J
023047   B6N.FVB-Tg(Dmp1-cre)1Jqfe/BwdJ
017927   B6N.FVB-Tg(Mpz-cre)26Mes/J
003465   BALB/c-Tg(CMV-cre)1Cgn/J
012641   BALB/c-Tg(S100a4-cre)1Egn/YunkJ
010612   C.129P2(Cg)-Ighg1tm1(IRES-cre)Cgn/J
017353   C.129S4(B6)-Il13tm1(YFP/cre)Lky/J
017582   C.129S4(B6)-Mcpt8tm1(cre)Lky/J
004126   C.Cg-Cd19tm1(cre)Cgn Ighb/J
005673   C.Cg-Tg(Mx1-cre)1Cgn/J
006244   C.Cg-Tg(tetO-cre)1Jaw/J
009155   C57BL/6-Cldn6tm1(cre)Dkwu/J
017557   C57BL/6-Tg(BEST1-cre)1Jdun/J
027406   C57BL/6-Tg(CD2-cre)1Lov/J
016097   C57BL/6-Tg(Car1-cre)5Flt/J
011086   C57BL/6-Tg(Cck-cre)CKres/J
008766   C57BL/6-Tg(Cd8a-cre)1Itan/J
026828   C57BL/6-Tg(Cpa3-cre)4Glli/J
006474   C57BL/6-Tg(Grik4-cre)G32-4Stl/J
008314   C57BL/6-Tg(HBB-cre)12Kpe/J
008870   C57BL/6-Tg(Hspa2-cre)1Eddy/J
016261   C57BL/6-Tg(Nes-cre/ERT2)KEisc/J
012906   C57BL/6-Tg(Nes-cre/Esr1*)1Kuan/J
027205   C57BL/6-Tg(Nms-icre)20Ywa/J
016617   C57BL/6-Tg(Nr4a1-EGFP/cre)820Khog/J
020287   C57BL/6-Tg(Pbsn-cre/Esr1*)14Abch/J
013148   C57BL/6-Tg(Pdgfra-cre)1Clc/J
024034   C57BL/6-Tg(Pmch-cre)1Rck/J
016583   C57BL/6-Tg(Slc6a3-icre/ERT2)2Gloss/J
006888   C57BL/6-Tg(Zp3-cre)1Gwh/J
003651   C57BL/6-Tg(Zp3-cre)93Knw/J
021119   C57BL/6J-Tg(Dlx2-cre,-mCherry)4Grsr/GrsrJ
021423   C57BL/6J-Tg(Dlx2-cre,-mCherry)9Grsr/GrsrJ
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
018895   C57BL/6J-Tg(Krt6,-cre,-Cerulean)1Grsr/Grsr
018896   C57BL/6J-Tg(Krt6,-cre,-Cerulean)2Grsr/Grsr
018898   C57BL/6J-Tg(Krt6,-cre,-Cerulean)4Grsr/Grsr
018899   C57BL/6J-Tg(Krt6,-cre,-Cerulean)5Grsr/Grsr
021582   C57BL/6J-Tg(Mchr1-cre)1Emf/J
008661   C57BL/6J-Tg(Nkx2-1-cre)2Sand/J
022883   C57BL/6J-Tg(Six6-cre)3Grsr/GrsrJ
022887   C57BL/6J-Tg(Six6-cre)7Grsr/GrsrJ
018754   C57BL/6J-Tg(Tbx22,-cre,-mCherry)1Grsr/GrsrJ
019363   C57BL/6J-Tg(Trp63,-cre,-Cerulean)10Grsr/Grsr
018792   C57BL/6J-Tg(Trp63,-cre,-Cerulean)4Grsr/GrsrJ
003650   C57BL/6J-Tg(Zp3-cre)82Knw/KnwJ
018151   C57BL/6N-Krt17tm1(cre,Cerulean)Murr/GrsrJ
023014   C57BL/6N-Tg(Calcrl,cre)4688Nkza/J
012686   C57BL/6N-Tg(Ppp1r2-cre)4127Nkza/J
016582   C57BL/6N-Tg(Slc32a1-icre/ERT2)3Gloss/J
026861   D2.129P2(B6)-Lyz2tm1(cre)Ifo/SjJ
026858   D2.129S4(B6)-Meox2tm1(cre)Sor/SjJ
026266   D2.B6-Tg(Zp3-cre)93Knw/SjJ
026852   D2.Cg-Tg(Gfap-cre)73.12Mvs/SjJ
024701   D2.Cg-Tg(Plp1-cre/ERT)3Pop/SjJ
026859   D2.Cg-Tg(Sox2-cre)1Amc/SjJ
026857   D2.FVB-Tg(GFAP-cre)25Mes/SjJ
026860   D2.FVB-Tg(Tek-cre)2352Rwng/SjJ
016833   FVB(Cg)-Tg(Alb1-cre)1Dlr/J
012929   FVB(Cg)-Tg(Dhh-cre)1Mejr/J
011034   FVB(Cg)-Tg(Ghrhr-cre)3242Lsk/J
006405   FVB-Tg(Ckmm-cre)5Khn/J
021024   FVB-Tg(Csf1r-icre)1Jwp/J
006954   FVB-Tg(Ddx4-cre)1Dcas/J
004600   FVB-Tg(GFAP-cre)25Mes/J
011037   FVB-Tg(Myh6-cre)2182Mds/J
006364   FVB-Tg(Nr5a1-cre)2Lowl/J
008537   FVB-Tg(Tek-cre)2352Rwng/J
019382   FVB.Cg-Myh9tm1.1Gac Tg(NPHS2-cre)295Lbh/Mmjax
014140   FVB.Cg-Myod1tm2.1(icre)Glh/J
006139   FVB.Cg-Tg(ACTA1-cre)79Jme/J
006297   FVB.Cg-Tg(Eno2-cre)39Jme/J
018394   FVB.Cg-Tg(KRT5-cre/ERT2)2Ipc/JeldJ
008244   FVB.Cg-Tg(tetO-cre)1Jaw/J
003376   FVB/N-Tg(ACTB-cre)2Mrt/J
024384   FVB/N-Tg(AMELX-cre)A1Kul/J
003314   FVB/N-Tg(EIIa-cre)C5379Lmgd/J
025062   FVB/N-Tg(Figla-EGFP,-icre)ZP3Dean/Mmjax
017928   FVB/N-Tg(Mpz-cre)26Mes/J
025066   FVB/N-Tg(Mylpf-cre)3Kraj/Mmjax
006143   FVB/N-Tg(Thy1-cre)1Vln/J
003377   FVB/N-Tg(Zp3-cre)3Mrt/J
023325   FVB;B6-Tg(Pbsn-cre)20Fwan/J
019096   NOD.129P2(B6)-Lyz2tm1(cre)Ifo/NadlJ
023806   NOD.129P2(Cg)-Cd19tm1(cre)Cgn/J
013233   NOD.B6-Tg(Itgax-cre,-EGFP)4097Ach/J
013234   NOD.Cg-Tg(Cd4-cre)1Cwi/2AchJ
023972   NOD.Cg-Tg(Ins2-cre/ERT)1Dam/SbwJ
023203   NOD.Cg-Tg(Itgax-cre)1-1Reiz/PesaJ
005732   NOD.Cg-Tg(Lck-cre)548Jxm/AchJ
023973   NOD.Cg-Tg(Neurog3-cre)1Dam/SbwJ
013251   NOD.FVB-Tg(EIIa-cre)C5379Lmgd/J
008694   NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J
004986   NOD/ShiLt-Tg(Ins2-cre)3Lt/LtJ
003855   NOD/ShiLt-Tg(Ins2-cre)5Lt/LtJ
004987   NOD/ShiLt-Tg(Ins2-cre)6Lt/LtJ
012899   STOCK Agrptm1(cre)Lowl/J
026229   STOCK Akap12tm1Ihg Rb1tm2Brn Tg(Pbsn-cre)4Prb/J
012706   STOCK Ccktm1.1(cre)Zjh/J
010910   STOCK Corttm1(cre)Zjh/J
007916   STOCK En1tm2(cre)Wrst/J
008464   STOCK Foxa2tm2.1(cre/Esr1*)Moon/J
010802   STOCK Gad2tm2(cre)Zjh/J
018903   STOCK Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
023407   STOCK HhatTg(TFAP2A-cre)1Will/J
008876   STOCK Hprttm11(Ple176-EGFP/cre)Ems/Mmjax
016879   STOCK Il17atm1.1(icre)Stck/J
024242   STOCK Isl1tm1(cre)Sev/J
018976   STOCK Kdrtm1(cre)Sato/J
017701   STOCK Kiss1tm1.1(cre/EGFP)Stei/J
007022   STOCK Mnx1tm4(cre)Tmj Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb/J
004192   STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J
023342   STOCK Myf5tm1(cre/Esr1*)Trdo/J
024713   STOCK Myl1tm1(cre)Sjb/J
014180   STOCK Myocdtm1(cre)Jomm/J
006953   STOCK Notch1tm3(cre)Rko/J
006677   STOCK Olfr151tm28(cre)Mom/MomJ
011103   STOCK Olig2tm2(TVA,cre)Rth/J
010530   STOCK Pax7tm1(cre)Mrc/J
016963   STOCK Slc17a6tm2(cre)Lowl/J
016962   STOCK Slc32a1tm2(cre)Lowl/J
013044   STOCK Ssttm2.1(cre)Zjh/J
012719   STOCK Tgfb3tm1(cre)Vk/J
012620   STOCK Trp53tm1Brd Brca1tm1Aash Tg(LGB-cre)74Acl/J
010908   STOCK Viptm1(cre)Zjh/J
010911   STOCK Wt1tm1(EGFP/cre)Wtp/J
009615   STOCK Tg(Cartpt-cre)1Aibs/J
017336   STOCK Tg(Cd4-cre)1Cwi/BfluJ
005105   STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J
008852   STOCK Tg(En2-cre)22Alj/J
005938   STOCK Tg(Eno2-cre)39Jme/J
011062   STOCK Tg(Gdf9-cre)5092Coo/J
012841   STOCK Tg(Ggt1-cre)M3Egn/J
021207   STOCK Tg(Gnrh1-cre)1Dlc/J
017981   STOCK Tg(Hoxb6-cre)#Mku/J
004692   STOCK Tg(Hoxb7-cre)13Amc/J
014600   STOCK Tg(I12b-cre/ERT2,-ALPP)37Fsh/J
008122   STOCK Tg(Ins2-cre/ERT)1Dam/J
004782   STOCK Tg(KRT14-cre)1Amc/J
005107   STOCK Tg(KRT14-cre/ERT)20Efu/J
008582   STOCK Tg(Kcnc2-Cre)K128Stl/LetJ
023426   STOCK Tg(Kiss1-cre)J2-4Cfe/J
017836   STOCK Tg(LGB-cre)74Acl/J
003551   STOCK Tg(MMTV-cre)1Mam/J
003553   STOCK Tg(MMTV-cre)4Mam/J
002527   STOCK Tg(Mx1-cre)1Cgn/J
009074   STOCK Tg(Myh6-cre)1Jmk/J
005650   STOCK Tg(Myh6-cre/Esr1*)1Jmk/J
009102   STOCK Tg(Nefh-cre)12Kul/J
002858   STOCK Tg(Nes-cre)1Wme/J
002859   STOCK Tg(Nes-cre)2Wme/J
012859   STOCK Tg(Neurog1-cre)1Jejo/J
005667   STOCK Tg(Neurog3-cre)C1Able/J
008119   STOCK Tg(Neurog3-cre/Esr1*)1Dam/J
012462   STOCK Tg(Nr5a1-cre)7Lowl/J
014158   STOCK Tg(Pax4-cre)1Dam/J
024578   STOCK Tg(Pax6-GFP/cre)1Rilm/J
006207   STOCK Tg(Pcp2-cre)1Amc/J
014099   STOCK Tg(Pmch-cre)1Lowl/J
005965   STOCK Tg(Pomc1-cre)16Lowl/J
012452   STOCK Tg(Rr5-GFP/cre)1Sapc/J
006395   STOCK Tg(Sim1-cre)1Lowl/J
009606   STOCK Tg(Six2-EGFP/cre)1Amc/J
019755   STOCK Tg(Six3-cre)69Frty/GcoJ
018147   STOCK Tg(Slc17a8-icre)1Edw/SealJ
012586   STOCK Tg(Slc1a3-cre/ERT)1Nat/J
004783   STOCK Tg(Sox2-cre)1Amc/J
008208   STOCK Tg(Stra8-icre)1Reb/J
016236   STOCK Tg(TCF/Lef1-cre/ERT2)1Dje/J
004746   STOCK Tg(Tagln-cre)1Her/J
012708   STOCK Tg(Thy1-cre/ERT2,-EYFP)HGfng/PyngJ
024240   STOCK Tg(Tnnt2-cre)5Blh/JiaoJ
016584   STOCK Tg(Tph2-icre/ERT2)6Gloss/J
003829   STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J
008851   STOCK Tg(Wnt1-cre/ERT)1Alj/J
018281   STOCK Tg(Wnt7a-EGFP/cre)#Bhr/Mmjax
008199   STOCK Tg(dlx6a-cre)1Mekk/J
002471   STOCK Tg(hCMV-cre)140Sau/J
023724   STOCK Tg(mI56i-cre,EGFP)1Kc/J
006224   STOCK Tg(tetO-cre)1Jaw/J
View Strains carrying other alleles of cre     (402 strains)

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Introduction to Cre-lox technology


Phenotype Information

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      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol Tg(Pf4-cre)Q3Rsko
Allele Name transgene insertion Q3, Radek C Skoda
Allele Type Transgenic (cre- or Flp-expressing)
Common Name(s) Pf4-Cre; Tg(Cxcl4-cre)Q3Rsko;
Mutation Made By Radek Skoda,   University Hospital Basel
Strain of OriginC57BL/6
Site of ExpressionCre is detected in the majority of megakaryocytes.
Expressed Gene cre, cre recombinase, bacteriophage P1
Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence.
Promoter Pf4, platelet factor 4, mouse, laboratory
Driver Note Pf4
Molecular Note A transgene was created from a BAC where cre-recombinase is driven by the Cxcl4 (Platelet factor 4) promoter. This transgene also contains the Cxcl3, Cxcl5, Ppbp, and Cxcl15 genes. This line has one copy of the transgene inserted with expression restricted to megakaryocytes and platelets, as demonstrated by RT-PCR and Western blotting. [MGI Ref ID J:127229]


Genotyping Information

Genotyping Protocols

Tg(Pf4-cre)Q3Rsko alternate1, High Resolution Melting

Helpful Links

Genotyping resources and troubleshooting


References provided by MGI

Selected Reference(s)

Tiedt R; Schomber T; Hao-Shen H; Skoda RC. 2007. Pf4-Cre transgenic mice allow the generation of lineage-restricted gene knockouts for studying megakaryocyte and platelet function in vivo. Blood 109(4):1503-6. [PubMed: 17032923]  [MGI Ref ID J:127229]

Additional References

Tg(Pf4-cre)Q3Rsko related

Barata L; Miwa T; Sato S; Kim D; Mohammed I; Song WC. 2013. Deletion of Crry and DAF on Murine Platelets Stimulates Thrombopoiesis and Increases Factor H-Dependent Resistance of Peripheral Platelets to Complement Attack. J Immunol 190(6):2886-95. [PubMed: 23390291]  [MGI Ref ID J:193605]

Bender M; Eckly A; Hartwig JH; Elvers M; Pleines I; Gupta S; Krohne G; Jeanclos E; Gohla A; Gurniak C; Gachet C; Witke W; Nieswandt B. 2010. ADF/n-cofilin-dependent actin turnover determines platelet formation and sizing. Blood 116(10):1767-75. [PubMed: 20530287]  [MGI Ref ID J:164521]

Bender M; Giannini S; Grozovsky R; Jonsson T; Christensen H; Pluthero FG; Ko A; Mullally A; Kahr WH; Hoffmeister KM; Falet H. 2015. Dynamin 2-dependent endocytosis is required for normal megakaryocyte development in mice. Blood 125(6):1014-24. [PubMed: 25468568]  [MGI Ref ID J:221386]

Bender M; Hofmann S; Stegner D; Chalaris A; Bosl M; Braun A; Scheller J; Rose-John S; Nieswandt B. 2010. Differentially regulated GPVI ectodomain shedding by multiple platelet-expressed proteinases. Blood 116(17):3347-55. [PubMed: 20644114]  [MGI Ref ID J:165863]

Bender M; May F; Lorenz V; Thielmann I; Hagedorn I; Finney BA; Vogtle T; Remer K; Braun A; Bosl M; Watson SP; Nieswandt B. 2013. Combined in vivo depletion of glycoprotein VI and C-type lectin-like receptor 2 severely compromises hemostasis and abrogates arterial thrombosis in mice. Arterioscler Thromb Vasc Biol 33(5):926-34. [PubMed: 23448972]  [MGI Ref ID J:217963]

Benezech C; Nayar S; Finney BA; Withers DR; Lowe K; Desanti GE; Marriott CL; Watson SP; Caamano JH; Buckley CD; Barone F. 2014. CLEC-2 is required for development and maintenance of lymph nodes. Blood 123(20):3200-7. [PubMed: 24532804]  [MGI Ref ID J:210759]

Bertozzi CC; Schmaier AA; Mericko P; Hess PR; Zou Z; Chen M; Chen CY; Xu B; Lu MM; Zhou D; Sebzda E; Santore MT; Merianos DJ; Stadtfeld M; Flake AW; Graf T; Skoda R; Maltzman JS; Koretzky GA; Kahn ML. 2010. Platelets regulate lymphatic vascular development through CLEC-2-SLP-76 signaling. Blood 116(4):661-70. [PubMed: 20363774]  [MGI Ref ID J:162815]

Bhavaraju K; Lakhani PR; Dorsam RT; Jin J; Hitchcock IS; Sanjay A; Kunapuli SP. 2011. G(12/13) signaling pathways substitute for integrin alphaIIbbeta3-signaling for thromboxane generation in platelets. PLoS One 6(2):e16586. [PubMed: 21347357]  [MGI Ref ID J:171092]

Calaminus SD; Guitart A; Sinclair A; Schachtner H; Watson SP; Holyoake TL; Kranc KR; Machesky LM. 2012. Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. PLoS One 7(12):e51361. [PubMed: 23300543]  [MGI Ref ID J:195733]

Carramolino L; Fuentes J; Garcia-Andres C; Azcoitia V; Riethmacher D; Torres M. 2010. Platelets play an essential role in separating the blood and lymphatic vasculatures during embryonic angiogenesis. Circ Res 106(7):1197-201. [PubMed: 20203303]  [MGI Ref ID J:171033]

Chen X; Zhang Y; Wang Y; Li D; Zhang L; Wang K; Luo X; Yang Z; Wu Y; Liu J. 2013. PDK1 regulates platelet activation and arterial thrombosis. Blood 121(18):3718-26. [PubMed: 23444402]  [MGI Ref ID J:197452]

Debrincat MA; Josefsson EC; James C; Henley KJ; Ellis S; Lebois M; Betterman KL; Lane RM; Rogers KL; White MJ; Roberts AW; Harvey NL; Metcalf D; Kile BT. 2012. Mcl-1 and Bcl-x(L) coordinately regulate megakaryocyte survival. Blood 119(24):5850-8. [PubMed: 22374700]  [MGI Ref ID J:186573]

Devanathan V; Hagedorn I; Kohler D; Pexa K; Cherpokova D; Kraft P; Singh M; Rosenberger P; Stoll G; Birnbaumer L; Piekorz RP; Beer-Hammer S; Nieswandt B; Nurnberg B. 2015. Platelet Gi protein Galphai2 is an essential mediator of thrombo-inflammatory organ damage in mice. Proc Natl Acad Sci U S A 112(20):6491-6. [PubMed: 25944935]  [MGI Ref ID J:221651]

Eckly A; Strassel C; Freund M; Cazenave JP; Lanza F; Gachet C; Leon C. 2009. Abnormal megakaryocyte morphology and proplatelet formation in mice with megakaryocyte-restricted MYH9 inactivation. Blood 113(14):3182-9. [PubMed: 18984861]  [MGI Ref ID J:148137]

Etheridge SL; Roh ME; Cosgrove ME; Sangkhae V; Fox NE; Chen J; Lopez JA; Kaushansky K; Hitchcock IS. 2014. JAK2V617F-positive endothelial cells contribute to clotting abnormalities in myeloproliferative neoplasms. Proc Natl Acad Sci U S A 111(6):2295-300. [PubMed: 24469804]  [MGI Ref ID J:206659]

Finney BA; Schweighoffer E; Navarro-Nunez L; Benezech C; Barone F; Hughes CE; Langan SA; Lowe KL; Pollitt AY; Mourao-Sa D; Sheardown S; Nash GB; Smithers N; Reis e Sousa C; Tybulewicz VL; Watson SP. 2012. CLEC-2 and Syk in the megakaryocytic/platelet lineage are essential for development. Blood 119(7):1747-56. [PubMed: 22186994]  [MGI Ref ID J:181715]

Gambaryan S; Friebe A; Walter U. 2012. Does the NO/sGC/cGMP/PKG pathway play a stimulatory role in platelets? Blood 119(22):5335-6. [PubMed: 22653960]  [MGI Ref ID J:185197]

Habart D; Cheli Y; Nugent DJ; Ruggeri ZM; Kunicki TJ. 2013. Conditional knockout of integrin alpha2beta1 in murine megakaryocytes leads to reduced mean platelet volume. PLoS One 8(1):e55094. [PubMed: 23359821]  [MGI Ref ID J:195799]

Halene S; Gao Y; Hahn K; Massaro S; Italiano JE Jr; Schulz V; Lin S; Kupfer GM; Krause DS. 2010. Serum response factor is an essential transcription factor in megakaryocytic maturation. Blood 116(11):1942-50. [PubMed: 20525922]  [MGI Ref ID J:164524]

Haling JR; Monkley SJ; Critchley DR; Petrich BG. 2011. Talin-dependent integrin activation is required for fibrin clot retraction by platelets. Blood 117(5):1719-22. [PubMed: 20971947]  [MGI Ref ID J:177971]

Herzog BH; Fu J; Wilson SJ; Hess PR; Sen A; McDaniel JM; Pan Y; Sheng M; Yago T; Silasi-Mansat R; McGee S; May F; Nieswandt B; Morris AJ; Lupu F; Coughlin SR; McEver RP; Chen H; Kahn ML; Xia L. 2013. Podoplanin maintains high endothelial venule integrity by interacting with platelet CLEC-2. Nature 502(7469):105-9. [PubMed: 23995678]  [MGI Ref ID J:205423]

Hitchcock IS; Fox NE; Prevost N; Sear K; Shattil SJ; Kaushansky K. 2008. Roles of focal adhesion kinase (FAK) in megakaryopoiesis and platelet function: studies using a megakaryocyte lineage specific FAK knockout. Blood 111(2):596-604. [PubMed: 17925492]  [MGI Ref ID J:130102]

Horrillo A; Fontela T; Arias-Salgado EG; Llobat D; Porras G; Ayuso MS; Gonzalez-Manchon C. 2014. Generation of mice with conditional ablation of the Cd40lg gene: new insights on the role of CD40L. Transgenic Res 23(1):53-66. [PubMed: 24030045]  [MGI Ref ID J:213459]

Huang H; Woo AJ; Waldon Z; Schindler Y; Moran TB; Zhu HH; Feng GS; Steen H; Cantor AB. 2012. A Src family kinase-Shp2 axis controls RUNX1 activity in megakaryocyte and T-lymphocyte differentiation. Genes Dev 26(14):1587-601. [PubMed: 22759635]  [MGI Ref ID J:185343]

Josefsson EC; Burnett DL; Lebois M; Debrincat MA; White MJ; Henley KJ; Lane RM; Moujalled D; Preston SP; O'Reilly LA; Pellegrini M; Metcalf D; Strasser A; Kile BT. 2014. Platelet production proceeds independently of the intrinsic and extrinsic apoptosis pathways. Nat Commun 5:3455. [PubMed: 24632563]  [MGI Ref ID J:210320]

Josefsson EC; James C; Henley KJ; Debrincat MA; Rogers KL; Dowling MR; White MJ; Kruse EA; Lane RM; Ellis S; Nurden P; Mason KD; O'Reilly LA; Roberts AW; Metcalf D; Huang DC; Kile BT. 2011. Megakaryocytes possess a functional intrinsic apoptosis pathway that must be restrained to survive and produce platelets. J Exp Med 208(10):2017-31. [PubMed: 21911424]  [MGI Ref ID J:177288]

Jurak Begonja A; Hoffmeister KM; Hartwig JH; Falet H. 2011. FlnA-null megakaryocytes prematurely release large and fragile platelets that circulate poorly. Blood 118(8):2285-95. [PubMed: 21652675]  [MGI Ref ID J:176939]

Karim ZA; Zhang J; Banerjee M; Chicka MC; Al Hawas R; Hamilton TR; Roche PA; Whiteheart SW. 2013. IkappaB kinase phosphorylation of SNAP-23 controls platelet secretion. Blood 121(22):4567-74. [PubMed: 23613522]  [MGI Ref ID J:198957]

Kim K; Hahm E; Li J; Holbrook LM; Sasikumar P; Stanley RG; Ushio-Fukai M; Gibbins JM; Cho J. 2013. Platelet protein disulfide isomerase is required for thrombus formation but not for hemostasis in mice. Blood 122(6):1052-61. [PubMed: 23788140]  [MGI Ref ID J:202279]

Kodama T; Hikita H; Kawaguchi T; Shigekawa M; Shimizu S; Hayashi Y; Li W; Miyagi T; Hosui A; Tatsumi T; Kanto T; Hiramatsu N; Kiyomizu K; Tadokoro S; Tomiyama Y; Hayashi N; Takehara T. 2012. Mcl-1 and Bcl-xL regulate Bak/Bax-dependent apoptosis of the megakaryocytic lineage at multistages. Cell Death Differ 19(11):1856-69. [PubMed: 22790873]  [MGI Ref ID J:205468]

Kodama T; Takehara T; Hikita H; Shimizu S; Shigekawa M; Li W; Miyagi T; Hosui A; Tatsumi T; Ishida H; Kanto T; Hiramatsu N; Yin XM; Hayashi N. 2011. BH3-only Activator Proteins Bid and Bim Are Dispensable for Bak/Bax-dependent Thrombocyte Apoptosis Induced by Bcl-xL Deficiency: MOLECULAR REQUISITES FOR THE MITOCHONDRIAL PATHWAY TO APOPTOSIS IN PLATELETS. J Biol Chem 286(16):13905-13. [PubMed: 21367852]  [MGI Ref ID J:171123]

Laurent PA; Severin S; Hechler B; Vanhaesebroeck B; Payrastre B; Gratacap MP. 2015. Platelet PI3Kbeta and GSK3 regulate thrombus stability at a high shear rate. Blood 125(5):881-8. [PubMed: 25398937]  [MGI Ref ID J:221208]

Leon C; Eckly A; Hechler B; Aleil B; Freund M; Ravanat C; Jourdain M; Nonne C; Weber J; Tiedt R; Gratacap MP; Severin S; Cazenave JP; Lanza F; Skoda R; Gachet C. 2007. Megakaryocyte-restricted MYH9 inactivation dramatically affects hemostasis while preserving platelet aggregation and secretion. Blood 110(9):3183-91. [PubMed: 17664350]  [MGI Ref ID J:148233]

Liu F; Gamez G; Myers DR; Clemmons W; Lam WA; Jobe SM. 2013. Mitochondrially mediated integrin alphaIIbbeta3 protein inactivation limits thrombus growth. J Biol Chem 288(42):30672-81. [PubMed: 24014035]  [MGI Ref ID J:204912]

Martin V; Guillermet-Guibert J; Chicanne G; Cabou C; Jandrot-Perrus M; Plantavid M; Vanhaesebroeck B; Payrastre B; Gratacap MP. 2010. Deletion of the p110beta isoform of phosphoinositide 3-kinase in platelets reveals its central role in Akt activation and thrombus formation in vitro and in vivo. Blood 115(10):2008-13. [PubMed: 20065293]  [MGI Ref ID J:158839]

Mazharian A; Mori J; Wang YJ; Heising S; Neel BG; Watson SP; Senis YA. 2013. Megakaryocyte-specific deletion of the protein-tyrosine phosphatases Shp1 and Shp2 causes abnormal megakaryocyte development, platelet production, and function. Blood 121(20):4205-20. [PubMed: 23509158]  [MGI Ref ID J:197924]

Meyer A; Wang W; Qu J; Croft L; Degen JL; Coller BS; Ahamed J. 2012. Platelet TGF-beta1 contributions to plasma TGF-beta1, cardiac fibrosis, and systolic dysfunction in a mouse model of pressure overload. Blood 119(4):1064-74. [PubMed: 22134166]  [MGI Ref ID J:181748]

Meyer SC; Keller MD; Woods BA; LaFave LM; Bastian L; Kleppe M; Bhagwat N; Marubayashi S; Levine RL. 2014. Genetic studies reveal an unexpected negative regulatory role for Jak2 in thrombopoiesis. Blood 124(14):2280-4. [PubMed: 25115888]  [MGI Ref ID J:218722]

Mitsios JV; Prevost N; Kasirer-Friede A; Gutierrez E; Groisman A; Abrams CS; Wang Y; Litvinov RI; Zemljic-Harpf A; Ross RS; Shattil SJ. 2010. What is vinculin needed for in platelets? J Thromb Haemost 8(10):2294-304. [PubMed: 20670372]  [MGI Ref ID J:182427]

Nakamura-Ishizu A; Takubo K; Fujioka M; Suda T. 2014. Megakaryocytes are essential for HSC quiescence through the production of thrombopoietin. Biochem Biophys Res Commun 454(2):353-7. [PubMed: 25451253]  [MGI Ref ID J:220268]

Ng AP; Kauppi M; Metcalf D; Hyland CD; Josefsson EC; Lebois M; Zhang JG; Baldwin TM; Di Rago L; Hilton DJ; Alexander WS. 2014. Mpl expression on megakaryocytes and platelets is dispensable for thrombopoiesis but essential to prevent myeloproliferation. Proc Natl Acad Sci U S A 111(16):5884-9. [PubMed: 24711413]  [MGI Ref ID J:208120]

Osada M; Inoue O; Ding G; Shirai T; Ichise H; Hirayama K; Takano K; Yatomi Y; Hirashima M; Fujii H; Suzuki-Inoue K; Ozaki Y. 2012. Platelet activation receptor CLEC-2 regulates blood/lymphatic vessel separation by inhibiting proliferation, migration, and tube formation of lymphatic endothelial cells. J Biol Chem 287(26):22241-52. [PubMed: 22556408]  [MGI Ref ID J:187533]

Pan Y; Yago T; Fu J; Herzog B; McDaniel JM; Mehta-D'Souza P; Cai X; Ruan C; McEver RP; West C; Dai K; Chen H; Xia L. 2014. Podoplanin requires sialylated O-glycans for stable expression on lymphatic endothelial cells and for interaction with platelets. Blood 124(24):3656-65. [PubMed: 25336627]  [MGI Ref ID J:220787]

Pawlinski R; Wang JG; Owens AP 3rd; Williams J; Antoniak S; Tencati M; Luther T; Rowley JW; Low EN; Weyrich AS; Mackman N. 2010. Hematopoietic and nonhematopoietic cell tissue factor activates the coagulation cascade in endotoxemic mice. Blood 116(5):806-14. [PubMed: 20410508]  [MGI Ref ID J:163510]

Pencovich N; Jaschek R; Dicken J; Amit A; Lotem J; Tanay A; Groner Y. 2013. Cell-autonomous function of Runx1 transcriptionally regulates mouse megakaryocytic maturation. PLoS One 8(5):e64248. [PubMed: 23717578]  [MGI Ref ID J:200717]

Pericacho M; Alonso-Martin S; Larrucea S; Gonzalez-Manchon C; Fernandez D; Sanchez I; Ayuso MS; Parrilla R. 2011. Diminished thrombogenic responses by deletion of the Podocalyxin Gene in mouse megakaryocytes. PLoS One 6(10):e26025. [PubMed: 22016802]  [MGI Ref ID J:178107]

Pertuy F; Eckly A; Weber J; Proamer F; Rinckel JY; Lanza F; Gachet C; Leon C. 2014. Myosin IIA is critical for organelle distribution and F-actin organization in megakaryocytes and platelets. Blood 123(8):1261-9. [PubMed: 24243973]  [MGI Ref ID J:208720]

Petrich BG; Marchese P; Ruggeri ZM; Spiess S; Weichert RA; Ye F; Tiedt R; Skoda RC; Monkley SJ; Critchley DR; Ginsberg MH. 2007. Talin is required for integrin-mediated platelet function in hemostasis and thrombosis. J Exp Med 204(13):3103-11. [PubMed: 18086863]  [MGI Ref ID J:129707]

Pleines I; Dutting S; Cherpokova D; Eckly A; Meyer I; Morowski M; Krohne G; Schulze H; Gachet C; Debili N; Brakebusch C; Nieswandt B. 2013. Defective tubulin organization and proplatelet formation in murine megakaryocytes lacking Rac1 and Cdc42. Blood 122(18):3178-87. [PubMed: 23861250]  [MGI Ref ID J:203299]

Pleines I; Eckly A; Elvers M; Hagedorn I; Eliautou S; Bender M; Wu X; Lanza F; Gachet C; Brakebusch C; Nieswandt B. 2010. Multiple alterations of platelet functions dominated by increased secretion in mice lacking Cdc42 in platelets. Blood 115(16):3364-73. [PubMed: 20139097]  [MGI Ref ID J:160792]

Pleines I; Hagedorn I; Gupta S; May F; Chakarova L; van Hengel J; Offermanns S; Krohne G; Kleinschnitz C; Brakebusch C; Nieswandt B. 2012. Megakaryocyte-specific RhoA deficiency causes macrothrombocytopenia and defective platelet activation in hemostasis and thrombosis. Blood 119(4):1054-63. [PubMed: 22045984]  [MGI Ref ID J:181662]

Porubsky S; Federico G; Muthing J; Jennemann R; Gretz N; Buttner S; Obermuller N; Jung O; Hauser IA; Grone E; Geiger H; Grone HJ; Betz C. 2014. Direct acute tubular damage contributes to Shigatoxin-mediated kidney failure. J Pathol 234(1):120-33. [PubMed: 24909663]  [MGI Ref ID J:213489]

Randriamboavonjy V; Isaak J; Elgheznawy A; Pistrosch F; Fromel T; Yin X; Badenhoop K; Heide H; Mayr M; Fleming I. 2012. Calpain inhibition stabilizes the platelet proteome and reactivity in diabetes. Blood 120(2):415-23. [PubMed: 22665935]  [MGI Ref ID J:189100]

Schaff M; Tang C; Maurer E; Bourdon C; Receveur N; Eckly A; Hechler B; Arnold C; de Arcangelis A; Nieswandt B; Denis CV; Lefebvre O; Georges-Labouesse E; Gachet C; Lanza F; Mangin PH. 2013. Integrin alpha6beta1 is the main receptor for vascular laminins and plays a role in platelet adhesion, activation, and arterial thrombosis. Circulation 128(5):541-52. [PubMed: 23797810]  [MGI Ref ID J:209493]

Shen B; Zhao X; O'Brien KA; Stojanovic-Terpo A; Delaney MK; Kim K; Cho J; Lam SC; Du X. 2013. A directional switch of integrin signalling and a new anti-thrombotic strategy. Nature 503(7474):131-5. [PubMed: 24162846]  [MGI Ref ID J:206751]

Shi DS; Smith MC; Campbell RA; Zimmerman PW; Franks ZB; Kraemer BF; Machlus KR; Ling J; Kamba P; Schwertz H; Rowley JW; Miles RR; Liu ZJ; Sola-Visner M; Italiano JE Jr; Christensen H; Kahr WH; Li DY; Weyrich AS. 2014. Proteasome function is required for platelet production. J Clin Invest 124(9):3757-66. [PubMed: 25061876]  [MGI Ref ID J:215666]

Shi G; Field DJ; Ko KA; Ture S; Srivastava K; Levy S; Kowalska MA; Poncz M; Fowell DJ; Morrell CN. 2014. Platelet factor 4 limits Th17 differentiation and cardiac allograft rejection. J Clin Invest 124(2):543-52. [PubMed: 24463452]  [MGI Ref ID J:208443]

Smith EC; Thon JN; Devine MT; Lin S; Schulz VP; Guo Y; Massaro SA; Halene S; Gallagher P; Italiano JE Jr; Krause DS. 2012. MKL1 and MKL2 play redundant and crucial roles in megakaryocyte maturation and platelet formation. Blood 120(11):2317-29. [PubMed: 22806889]  [MGI Ref ID J:189155]

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Suzuki A; Shin JW; Wang Y; Min SH; Poncz M; Choi JK; Discher DE; Carpenter CL; Lian L; Zhao L; Wang Y; Abrams CS. 2013. RhoA is essential for maintaining normal megakaryocyte ploidy and platelet generation. PLoS One 8(7):e69315. [PubMed: 23935982]  [MGI Ref ID J:204459]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   15-JAN-09
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHemizygous for Tg(Pf4-cre)Q3Rsko  
Price per Pair (US dollars $)Pair Genotype
$313.00Hemizygous for Tg(Pf4-cre)Q3Rsko x Noncarrier  
$313.00Noncarrier x Hemizygous for Tg(Pf4-cre)Q3Rsko  

Standard Supply

Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHemizygous for Tg(Pf4-cre)Q3Rsko  
Price per Pair (US dollars $)Pair Genotype
$406.90Hemizygous for Tg(Pf4-cre)Q3Rsko x Noncarrier  
$406.90Noncarrier x Hemizygous for Tg(Pf4-cre)Q3Rsko  

Standard Supply

Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

   000664 C57BL/6J
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use

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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty


In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.