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| Mice harboring the Tg(Tek-cre)2352Rwng (Tie2Cre) transgene express the Cre recombinase under the control of the mouse Tek, endothelial-specific receptor tyrosine kinase (also known as Tie2), promoter and may be useful for generating endothelial cell specific-targeted conditional mutations. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Coisogenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 21-APR-09 Species laboratory mouse Generation F?+ (20-APR-09) Donating Investigator Rong Wang, Univ of California Medical Center (UCSF) Description
These transgenic mice express the Cre recombinase under the control of the mouse Tek, endothelial-specific receptor tyrosine kinase (also known as Tie2), promoter. Cre recombinase expression is detected in most endothelial cells and blood islands of the extraembryonic mesoderm by embryonic day (ED)7.5 and in the dorsal aorta by ED8.5. Especially high levels of expression are seen in head vasculature by ED9. When crossed with reporter strains (expressing beta-galactosidase or alkaline phosphatase), recombination is detected in all blood vessels and some blood cells examined at ED11.5, which indicates that Cre activity occurs in early vascular progenitor cells, endothelial cells and some hematopoietic cells. Mice homozygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain represents an effective tool for generating endothelial cell specific-targeted conditional mutations.Development
A transgenic construct containing sequence encoding cre under the control of of the mouse Tek, endothelial-specific receptor tyrosine kinase (also known as Tie2), promoter was introduced into FVB/N donor eggs. Founder line 2352 was subsequently established.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Tek
004128 B6.Cg-Tg(Tek-cre)12Flv/J 008863 B6.Cg-Tg(Tek-cre)1Ywa/J 002856 FVB/N-Tg(TIE2-lacZ)182Sato/J 005707 STOCK Rag1tm1Mom Tg(TIE2-lacZ)182Sato/J 006770 STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J 003658 STOCK Tg(TIE2GFP)287Sato/J View Strains carrying other alleles of Tek (6 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Tg(Tek-cre)2352Rwng/0
involves: FVB/N
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:104401)
- hemizygous mice are viable and fertile and show no overt abnormalities
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cancer Research
endothelial cell marker for neovascularization
Cardiovascular Research
Cre-lox System
endothelial cell marker for neovascularization
Cre-lox System
Cre Recombinase Expression
Genetics Research
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: endothelial cell markers for neovascularization
Tissue/Cell Markers: endothelial cells
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Tek-cre)2352Rwng | ||
|---|---|---|---|
| Allele Name | transgene insertion 2352, Rong Wang | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Tg(Tek-cre)1Lfr; Tg(Tie2-Cre)2352; Tie2Cre; tie2-cre; | ||
| Mutation Made By | Christin Munkittrick, | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Tek, endothelial-specific receptor tyrosine kinase, mouse, laboratory | ||
| Driver Note | Tek | ||
| Molecular Note | A transgenic construct containing sequence encoding cre under the control of of the mouse Tek, endothelial-specific receptor tyrosine kinase (also known as Tie2), promoter was introduced into FVB/N donor eggs. Founder line 2352 was subsequently established. Cre recombinase expression is detected in most endothelial cells and blood islands of the extraembryonic mesoderm by embryonic day (E)7.5, in the dorsal aorta by E8.5 and is very strong in head vasculature by E9. [MGI Ref ID J:104401] | ||
| Gene Symbol and Name | Tg(Tek-cre)2352Rwng, transgene insertion 2352, Rong Wang | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(Tek-cre)1Lfr; Tg(Tie2-Cre)2352; Tie2Cre; tie2-cre; | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Braren R; Hu H; Kim YH; Beggs HE; Reichardt LF; Wang R. 2006. Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation. J Cell Biol 172(1):151-62. [PubMed: 16391003] [MGI Ref ID J:104401]
Proctor JM; Zang K; Wang D; Wang R; Reichardt LF. 2005. Vascular development of the brain requires beta8 integrin expression in the neuroepithelium. J Neurosci 25(43):9940-8. [PubMed: 16251442] [MGI Ref ID J:102190]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice may be bred as homozygotes. Mating System Noncarrier x Hemizygote (Female x Male) 21-APR-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Tek-cre)2352Rwng
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Tek-cre)2352Rwng x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Tek-cre)2352Rwng
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Tek-cre)2352Rwng
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Tek-cre)2352Rwng x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Tek-cre)2352Rwng
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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