Strain Name:

B6.Cg-Tg(Cspg4-cre/Esr1*)BAkik/J

Stock Number:

008538

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
Common Names: NG2-CreERTM;    
These NG2CreERTM BAC transgenic mice express a tamoxifen-inducible Cre recombinase (CreERTM) under the control of the mouse NG2 (Cspg4) promoter/enhancer. When bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. These NG2CreERTM BAC transgenic mice may be useful for inducible Cre recombinase expression in NG2-expressing glia (polydendrocytes, oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs.

Description

Strain Information

Type Congenic; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   22-DEC-10
Specieslaboratory mouse
GenerationN7+N1F3 (11-DEC-13)
Generation Definitions
 
Donating Investigator Akiko Nishiyama,   University of Connecticut

Description
Mice hemizygous for the NG2CreERTM BAC transgene are viable and fertile, with the mouse NG2 (Cspg4) promoter/enhancer directing expression of a tamoxifen-inducible Cre recombinase (CreERTM). This CreERTM fusion protein is estrogen insensitive, and is only active when it binds the estrogen analog 4-hydroxytamoxifen (OHT) or tamoxifen. When these NG2CreERTM BAC transgenic mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. No background cre activity is reported in the absence of tamoxifen. Following tamoxifen administration, the majority of Cre recombinase activity is reported in NG2-expressing glia (polydendrocytes, oligodendrocyte progenitor cells). The donating investigator specifically reports that tamoxifen-induced Cre recombinase activity is observed throughout the postnatal central nervous system; including the gray and white matter of the brain, cerebellum and spinal cord, and vascular mural cells. In addition, tamoxifen-induced Cre recombinase activity is not found in astrocytes, resting microglia, or neurons before postnatal day (p)60 (and <1% of neurons when cre is induced at/after p60). The donating investigator summarizes the cre induction efficiency in postnatal tissues as 45-50% of NG2 cells in P2-P60 brain and spinal cord, and has not extensively studied this in embryonic tissues. With tamoxifen administration, the Cre recombinase activity of these NG2CreERTM BAC transgenic mice is similar to that observed for the NG2creBAC mice with the same NG2 promoter/enhancer driving a constitutively active Cre recombinase (Stock No. 008533).

The CreERTM fusion protein consists of Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.

Development
The 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences), was modified by inserting a CreERTM fusion gene (CreERTM; Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain) followed by a rabbit beta-globin polyA signal into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent translation from the BAC NG2 gene and to allow translation from the first ATG in the CreERTM sequence. DNA from a correctly modified BAC clone was used as the source for the NG2CreERTM BAC transgene; this ~219 kb sequence was linearized and microinjected into the pronucleus of fertilized oocytes from C57BL/6 or (C57BL/6 x SJL)F1 mice. Cre-positive founder mice were identified and bred to C57BL/6J mice. Mice derived from line B (NG2creERB) had no background cre activity in the absence of tamoxifen, with cre activity specifically in NG2-expressing cells after tamoxifen injection. NG2CreERTM BAC transgenic mice were backcrossed to C57BL/6J mice for six generations (and breeders may have been selected with high transgene expression levels) prior to sending to The Jackson Laboratory Repository. Upon arrival, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. During backcrossing, the Y chromosome is likely to have been fixed to the C57BL/6J genetic background.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cspg4
008533   B6;FVB-Tg(Cspg4-cre)1Akik/J
022735   FVB.Cg-Tg(Cspg4-EGFP*)HDbe/J
008241   STOCK Tg(Cspg4-DsRed.T1)1Akik/J
View Strains carrying other alleles of Cspg4     (3 strains)

View Strains carrying other alleles of cre/Esr1     (15 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Cre-lox System
      Cre Recombinase expression in neural tissue

Research Tools
Cre-lox System
      Cre Recombinase Expression
      Cre Recombinase Expression: Inducible
Developmental Biology Research
      Cre-lox System
Genetics Research
      Mutagenesis and Transgenesis: Cre-lox System
      Tissue/Cell Markers
      Tissue/Cell Markers: Cre-lox System
      Tissue/Cell Markers: glial cells
      Tissue/Cell Markers: glial cells, oligodendrocytes, Schwann cells
Neurobiology Research
      cell marker

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Cspg4-cre/Esr1*)BAkik
Allele Name transgene insertion B, Akiko Nishiyama
Allele Type Transgenic (Inducible, cre- or Flp-expressing)
Common Name(s) NG2-CreER; NG2-CreER; NG2creERTM; NG2creERB; NG2creERTM;
Mutation Made By Akiko Nishiyama,   University of Connecticut
Strain of OriginC57BL/6 or (C57BL/6 x SJL)F1
Site of Expression Induced Cre recombinase drives expression in NG2-expressing glia (polydendrocytes, oligodendrocyte progenitor cells) in the central nervous system and Cspg4-expressing cells in other organs.
Expressed Gene cre/Esr1, Cre recombinase and estrogen receptor 1 fusion gene,
Promoter Cspg4, chondroitin sulfate proteoglycan 4, mouse, laboratory
Driver Note Cspg4
Inducible Note induced by tamoxifen
Molecular Note The 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences), was modified by inserting a CreERTM fusion gene (cre recombinase fusedto a G525R mutant form of the mouse estrogen receptor ligand binding domain) followed by a rabbit beta-globin polyA signal, into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent translation from the BAC NG2gene and to allow translation from the first ATG in the CreERTM sequence. The ~219 kb BAC sequence was linearized and microinjected into the pronucleus of fertilized oocytes from C57BL/6 or (C57BL/6 x SJL)F1 mice. Mice derived from line B (NG2creERB) had no background cre activity in the absence of tamoxifen, with cre activity specifically in NG2-expressing cells after tamoxifen injection. [MGI Ref ID J:162529] [MGI Ref ID J:167902]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Generic Cre Melt Curve Analysis, Probe
Gt(ROSA)26Sor(CAG-tdTomato), MELT
Generic Cre, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Zhu X; Hill RA; Dietrich D; Komitova M; Suzuki R; Nishiyama A. 2011. Age-dependent fate and lineage restriction of single NG2 cells. Development 138(4):745-53. [PubMed: 21266410]  [MGI Ref ID J:167902]

Additional References

Tg(Cspg4-cre/Esr1*)BAkik related

Armulik A; Genove G; Betsholtz C. 2011. Pericytes: developmental, physiological, and pathological perspectives, problems, and promises. Dev Cell 21(2):193-215. [PubMed: 21839917]  [MGI Ref ID J:193884]

De Biase LM; Nishiyama A; Bergles DE. 2010. Excitability and synaptic communication within the oligodendrocyte lineage. J Neurosci 30(10):3600-11. [PubMed: 20219994]  [MGI Ref ID J:162529]

Feng J; Mantesso A; De Bari C; Nishiyama A; Sharpe PT. 2011. Dual origin of mesenchymal stem cells contributing to organ growth and repair. Proc Natl Acad Sci U S A 108(16):6503-8. [PubMed: 21464310]  [MGI Ref ID J:171368]

Galvao RP; Kasina A; McNeill RS; Harbin JE; Foreman O; Verhaak RG; Nishiyama A; Miller CR; Zong H. 2014. Transformation of quiescent adult oligodendrocyte precursor cells into malignant glioma through a multistep reactivation process. Proc Natl Acad Sci U S A 111(40):E4214-23. [PubMed: 25246577]  [MGI Ref ID J:216462]

Komitova M; Serwanski DR; Richard Lu Q; Nishiyama A. 2011. NG2 cells are not a major source of reactive astrocytes after neocortical stab wound injury. Glia 59(5):800-9. [PubMed: 21351161]  [MGI Ref ID J:169748]

Magnusson JP; Goritz C; Tatarishvili J; Dias DO; Smith EM; Lindvall O; Kokaia Z; Frisen J. 2014. A latent neurogenic program in astrocytes regulated by Notch signaling in the mouse. Science 346(6206):237-41. [PubMed: 25301628]  [MGI Ref ID J:217437]

Robins SC; Trudel E; Rotondi O; Liu X; Djogo T; Kryzskaya D; Bourque CW; Kokoeva MV. 2013. Evidence for NG2-glia derived, adult-born functional neurons in the hypothalamus. PLoS One 8(10):e78236. [PubMed: 24205170]  [MGI Ref ID J:209234]

Rock JR; Barkauskas CE; Cronce MJ; Xue Y; Harris JR; Liang J; Noble PW; Hogan BL. 2011. Multiple stromal populations contribute to pulmonary fibrosis without evidence for epithelial to mesenchymal transition. Proc Natl Acad Sci U S A 108(52):E1475-83. [PubMed: 22123957]  [MGI Ref ID J:180047]

Zhao H; Feng J; Seidel K; Shi S; Klein O; Sharpe P; Chai Y. 2014. Secretion of shh by a neurovascular bundle niche supports mesenchymal stem cell homeostasis in the adult mouse incisor. Cell Stem Cell 14(2):160-73. [PubMed: 24506883]  [MGI Ref ID J:210170]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, transgenic carrier mice may be bred together, to wildtype (noncarrier) mice from the colony, or to C57BL/6J inbred mice (Stock No. 000664).
Mating SystemNoncarrier x Hemizygote         (Female x Male)   22-DEC-10
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHemizygous for Tg(Cspg4-cre/Esr1*)BAkik  
Price per Pair (US dollars $)Pair Genotype
$313.00Hemizygous for Tg(Cspg4-cre/Esr1*)BAkik x Noncarrier  
$313.00Noncarrier x Hemizygous for Tg(Cspg4-cre/Esr1*)BAkik  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHemizygous for Tg(Cspg4-cre/Esr1*)BAkik  
Price per Pair (US dollars $)Pair Genotype
$406.90Hemizygous for Tg(Cspg4-cre/Esr1*)BAkik x Noncarrier  
$406.90Noncarrier x Hemizygous for Tg(Cspg4-cre/Esr1*)BAkik  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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