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| These NG2CreERTM BAC transgenic mice express a tamoxifen-inducible Cre recombinase (CreERTM) under the control of the mouse NG2 (Cspg4) promoter/enhancer. When bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. These NG2CreERTM BAC transgenic mice may be useful for inducible Cre recombinase expression in NG2-expressing glia (polydendrocytes, oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs. | ||||||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
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- References
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 22-DEC-10 Species laboratory mouse Generation N7+N1F1 (07-JUL-11)
Generation DefinitionsDonating Investigator Akiko Nishiyama, University of Connecticut Description
Mice hemizygous for the NG2CreERTM BAC transgene are viable and fertile, with the mouse NG2 (Cspg4) promoter/enhancer directing expression of a tamoxifen-inducible Cre recombinase (CreERTM). This CreERTM fusion protein is estrogen insensitive, and is only active when it binds the estrogen analog 4-hydroxytamoxifen (OHT) or tamoxifen. When these NG2CreERTM BAC transgenic mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. No background cre activity is reported in the absence of tamoxifen. Following tamoxifen administration, the majority of Cre recombinase activity is reported in NG2-expressing glia (polydendrocytes, oligodendrocyte progenitor cells). The donating investigator specifically reports that tamoxifen-induced Cre recombinase activity is observed throughout the postnatal central nervous system; including the gray and white matter of the brain, cerebellum and spinal cord, and vascular mural cells. In addition, tamoxifen-induced Cre recombinase activity is not found in astrocytes, resting microglia, or neurons before postnatal day (p)60 (and <1% of neurons when cre is induced at/after p60). The donating investigator summarizes the cre induction efficiency in postnatal tissues as 45-50% of NG2 cells in P2-P60 brain and spinal cord, and has not extensively studied this in embryonic tissues. With tamoxifen administration, the Cre recombinase activity of these NG2CreERTM BAC transgenic mice is similar to that observed for the NG2creBAC mice with the same NG2 promoter/enhancer driving a constitutively active Cre recombinase (Stock No. 008533).The CreERTM fusion protein consists of Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.
Development
The 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences), was modified by inserting a CreERTM fusion gene (CreERTM; Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain) followed by a rabbit beta-globin polyA signal into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent translation from the BAC NG2 gene and to allow translation from the first ATG in the CreERTM sequence. DNA from a correctly modified BAC clone was used as the source for the NG2CreERTM BAC transgene; this ~219 kb sequence was linearized and microinjected into the pronucleus of fertilized oocytes from C57BL/6 or (C57BL/6 x SJL)F1 mice. Cre-positive founder mice were identified and bred to C57BL/6J mice. Mice derived from line B (NG2creERB) had no background cre activity in the absence of tamoxifen, with cre activity specifically in NG2-expressing cells after tamoxifen injection. NG2CreERTM BAC transgenic mice were backcrossed to C57BL/6J mice for six generations (and breeders may have been selected with high transgene expression levels) prior to sending to The Jackson Laboratory Repository. Upon arrival, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. During backcrossing, the Y chromosome is likely to have been fixed to the C57BL/6J genetic background.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Cspg4
008533 B6;FVB-Tg(Cspg4-cre)1Akik/J 008241 STOCK Tg(Cspg4-DsRed.T1)1Akik/J View Strains carrying other alleles of Cspg4 (2 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Inducible
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: glial cells
Tissue/Cell Markers: glial cells, oligodendrocytes, Schwann cells
Neurobiology Research
cell marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Cspg4-cre/Esr1*)BAkik | ||
|---|---|---|---|
| Allele Name | transgene insertion B, Akiko Nishiyama | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | NG2-CreER; NG2creERTM; NG2creERB; NG2creERTM; | ||
| Mutation Made By | Akiko Nishiyama, University of Connecticut | ||
| Strain of Origin | C57BL/6 or (C57BL/6 x SJL)F1 | ||
| Site of Expression | Induced Cre recombinase drives expression in NG2-expressing glia (polydendrocytes, oligodendrocyte progenitor cells) in the central nervous system and Cspg4-expressing cells in other organs. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Cspg4, chondroitin sulfate proteoglycan 4, mouse, laboratory | ||
| Driver Note | Cspg4 | ||
| Inducible Note | induced by tamoxifen | ||
| Molecular Note | The 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences), was modified by inserting a CreERTM fusion gene (cre recombinase fusedto a G525R mutant form of the mouse estrogen receptor ligand binding domain) followed by a rabbit beta-globin polyA signal, into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent translation from the BAC NG2gene and to allow translation from the first ATG in the CreERTM sequence. The ~219 kb BAC sequence was linearized and microinjected into the pronucleus of fertilized oocytes from C57BL/6 or (C57BL/6 x SJL)F1 mice. Mice derived from line B (NG2creERB) had no background cre activity in the absence of tamoxifen, with cre activity specifically in NG2-expressing cells after tamoxifen injection. [MGI Ref ID J:162529] [MGI Ref ID J:167902] | ||
| Gene Symbol and Name | Tg(Cspg4-cre/Esr1*)BAkik, transgene insertion B, Akiko Nishiyama | ||
| Chromosome | UN | ||
| Gene Common Name(s) | NG2-CreER; NG2creERTM; NG2creERB; NG2creERTM; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Zhu X; Hill RA; Dietrich D; Komitova M; Suzuki R; Nishiyama A. 2011. Age-dependent fate and lineage restriction of single NG2 cells. Development 138(4):745-53. [PubMed: 21266410] [MGI Ref ID J:167902]
Additional References
Tg(Cspg4-cre/Esr1*)BAkik relatedArmulik A; Genove G; Betsholtz C. 2011. Pericytes: developmental, physiological, and pathological perspectives, problems, and promises. Dev Cell 21(2):193-215. [PubMed: 21839917] [MGI Ref ID J:193884]
De Biase LM; Nishiyama A; Bergles DE. 2010. Excitability and synaptic communication within the oligodendrocyte lineage. J Neurosci 30(10):3600-11. [PubMed: 20219994] [MGI Ref ID J:162529]
Feng J; Mantesso A; De Bari C; Nishiyama A; Sharpe PT. 2011. Dual origin of mesenchymal stem cells contributing to organ growth and repair. Proc Natl Acad Sci U S A 108(16):6503-8. [PubMed: 21464310] [MGI Ref ID J:171368]
Komitova M; Serwanski DR; Richard Lu Q; Nishiyama A. 2011. NG2 cells are not a major source of reactive astrocytes after neocortical stab wound injury. Glia 59(5):800-9. [PubMed: 21351161] [MGI Ref ID J:169748]
Rock JR; Barkauskas CE; Cronce MJ; Xue Y; Harris JR; Liang J; Noble PW; Hogan BL. 2011. Multiple stromal populations contribute to pulmonary fibrosis without evidence for epithelial to mesenchymal transition. Proc Natl Acad Sci U S A 108(52):E1475-83. [PubMed: 22123957] [MGI Ref ID J:180047]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic carrier mice may be bred together, to wildtype (noncarrier) mice from the colony, or to C57BL/6J inbred mice (Stock No. 000664). Mating System Noncarrier x Hemizygote (Female x Male) 22-DEC-10 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Cspg4-cre/Esr1*)BAkik
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Cspg4-cre/Esr1*)BAkik x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(Cspg4-cre/Esr1*)BAkik Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Cspg4-cre/Esr1*)BAkik
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Cspg4-cre/Esr1*)BAkik x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(Cspg4-cre/Esr1*)BAkik Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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