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| This strain expresses Cre recombinase from the targeted Alpl (alkaline phosphatase, liver/bone/kidney) locus. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the flanked sequence, primarily in embryonic primordial germ cells. This mutant mouse strain represents a model that may be useful in studies of reproductive and endocrine systems development. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
Strain Information
Type Coisogenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System +/+ sibling x Heterozygote (Female x Male) 20-JAN-09 Species laboratory mouse Generation N1+F7 (31-OCT-11)
Generation DefinitionsDonating Investigator Andras Nagy, Mount Sinai Hospital Description
This strain expresses Cre recombinase from the targeted locus. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the flanked sequence. Recombination occurs primarily in embryonic primordial germ cells. Approximately 60% of gonadal cells isolated from embryonic day 13.5 embryos exhibit Cre recombinase activity. Mosaic ectopic recombinase activity does occur. Homozygotes are not viable. This mutant mouse strain represents a model that may be useful in studies of reproductive and endocrine systems development.Development
A targeting vector containing cre coding sequence, IRES sequence and a neomycin resistance gene was inserted into exon 6 the targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+ derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were aggregated with ICR embryos and the resulting chimeric males were crossed to ICR females to test for germline transmission, after that identified chimeras-transmitters were crossed to 129S1 females to establish the line on 129 background. The strain was maintained by the donating investigator by crossing heterozygote males to 129S1 females. After arriving at The Jackson Laboratory the mice were crossed to 129S1/SvImJ.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Alpl
002484 129-Alpltm1Sor/J 002741 B6.129S7-Alpltm1Sor/J 002317 B6;129S7-Alpltm1Sor/J View Strains carrying other alleles of Alpl (3 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Germline/Embryonic Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Reproductive Biology Research
Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Alpltm1(cre)Nagy | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Andras Nagy | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Alpl-cre; TNAP-Cre; TNAP/Cre; TNAPCre; | ||
| Mutation Made By | Andras Nagy, Mount Sinai Hospital | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | embryonic primordial germ cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Driver Note | Alpl | ||
| Molecular Note | An IRES-cre gene followed by a neomycin gene was inserted into exon 6 of the gene. This allele expresses Cre recombinase in primordial germ cells. [MGI Ref ID J:60934] | ||
| Gene Symbol and Name | Alpl, alkaline phosphatase, liver/bone/kidney | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | AP-TNAP; APTNAP; Akp-2; Akp2; HOPS; PHOA; TNAP; TNSALP; alkaline phosphatase 2, liver; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Alplsuptm1(cre)Nagy/sup, Melt Curve Analysis
Alpltm1(cre)Nagy, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Lomeli H; Ramos-Mejia V; Gertsenstein M; Lobe CG; Nagy A. 2000. Targeted insertion of Cre recombinase into the TNAP gene: excision in primordial germ cells. Genesis 26(2):116-7. [PubMed: 10686602] [MGI Ref ID J:60934]
Additional References
Alpltm1(cre)Nagy relatedDubose AJ; Smith EY; Yang TP; Johnstone KA; Resnick JL. 2011. A new deletion refines the boundaries of the murine Prader-Willi syndrome imprinting center. Hum Mol Genet 20(17):3461-6. [PubMed: 21659337] [MGI Ref ID J:174457]
Elliott AM; de Miguel MP; Rebel VI; Donovan PJ. 2007. Identifying genes differentially expressed between PGCs and ES cells reveals a role for CREB-binding protein in germ cell survival. Dev Biol 311(2):347-58. [PubMed: 17936260] [MGI Ref ID J:127542]
Escalante-Alcalde D; Sanchez-Sanchez R; Stewart CL. 2007. Generation of a conditional Ppap2b/Lpp3 null allele. Genesis 45(7):465-9. [PubMed: 17610274] [MGI Ref ID J:125025]
Godmann M; Gashaw I; Katz JP; Nagy A; Kaestner KH; Behr R. 2009. Kruppel-like factor 4, a 'pluripotency transcription factor' highly expressed in male postmeiotic germ cells, is dispensable for spermatogenesis in the mouse. Mech Dev 126(8-9):650-64. [PubMed: 19539755] [MGI Ref ID J:152857]
Gu TP; Guo F; Yang H; Wu HP; Xu GF; Liu W; Xie ZG; Shi L; He X; Jin SG; Iqbal K; Shi YG; Deng Z; Szabo PE; Pfeifer GP; Li J; Xu GL. 2011. The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes. Nature 477(7366):606-10. [PubMed: 21892189] [MGI Ref ID J:176196]
Hayashi K; Chuva de Sousa Lopes SM; Kaneda M; Tang F; Hajkova P; Lao K; O'Carroll D; Das PP; Tarakhovsky A; Miska EA; Surani MA. 2008. MicroRNA Biogenesis Is Required for Mouse Primordial Germ Cell Development and Spermatogenesis. PLoS ONE 3(3):e1738. [PubMed: 18320056] [MGI Ref ID J:133129]
Hirasawa R; Chiba H; Kaneda M; Tajima S; Li E; Jaenisch R; Sasaki H. 2008. Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development. Genes Dev 22(12):1607-16. [PubMed: 18559477] [MGI Ref ID J:137187]
Hirota T; Ohta H; Shigeta M; Niwa H; Saitou M. 2011. Drug-inducible gene recombination by the Dppa3-MER Cre MER transgene in the developmental cycle of the germ cell lineage in mice. Biol Reprod 85(2):367-77. [PubMed: 21525417] [MGI Ref ID J:175289]
Holloway JK; Morelli MA; Borst PL; Cohen PE. 2010. Mammalian BLM helicase is critical for integrating multiple pathways of meiotic recombination. J Cell Biol 188(6):779-89. [PubMed: 20308424] [MGI Ref ID J:158806]
Inagaki T; Tachibana M; Magoori K; Kudo H; Tanaka T; Okamura M; Naito M; Kodama T; Shinkai Y; Sakai J. 2009. Obesity and metabolic syndrome in histone demethylase JHDM2a-deficient mice. Genes Cells 14(8):991-1001. [PubMed: 19624751] [MGI Ref ID J:158258]
Kaneda M; Okano M; Hata K; Sado T; Tsujimoto N; Li E; Sasaki H. 2004. Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting. Nature 429(6994):900-3. [PubMed: 15215868] [MGI Ref ID J:91108]
Kehler J; Tolkunova E; Koschorz B; Pesce M; Gentile L; Boiani M; Lomeli H; Nagy A; McLaughlin KJ; Scholer HR; Tomilin A. 2004. Oct4 is required for primordial germ cell survival. EMBO Rep 5(11):1078-83. [PubMed: 15486564] [MGI Ref ID J:95317]
Kidane D; Jonason AS; Gorton TS; Mihaylov I; Pan J; Keeney S; de Rooij DG; Ashley T; Keh A; Liu Y; Banerjee U; Zelterman D; Sweasy JB. 2010. DNA polymerase beta is critical for mouse meiotic synapsis. EMBO J 29(2):410-23. [PubMed: 20019666] [MGI Ref ID J:156442]
Kim S; Bardwell VJ; Zarkower D. 2007. Cell type-autonomous and non-autonomous requirements for Dmrt1 in postnatal testis differentiation. Dev Biol 307(2):314-27. [PubMed: 17540358] [MGI Ref ID J:122954]
Kimura T; Nakamura T; Murayama K; Umehara H; Yamano N; Watanabe S; Taketo MM; Nakano T. 2006. The stabilization of beta-catenin leads to impaired primordial germ cell development via aberrant cell cycle progression. Dev Biol 300(2):545-53. [PubMed: 17055474] [MGI Ref ID J:117138]
Kimura T; Suzuki A; Fujita Y; Yomogida K; Lomeli H; Asada N; Ikeuchi M; Nagy A; Mak TW; Nakano T. 2003. Conditional loss of PTEN leads to testicular teratoma and enhances embryonic germ cell production. Development 130(8):1691-700. [PubMed: 12620992] [MGI Ref ID J:82464]
Krentz AD; Murphy MW; Kim S; Cook MS; Capel B; Zhu R; Matin A; Sarver AL; Parker KL; Griswold MD; Looijenga LH; Bardwell VJ; Zarkower D. 2009. The DM domain protein DMRT1 is a dose-sensitive regulator of fetal germ cell proliferation and pluripotency. Proc Natl Acad Sci U S A 106(52):22323-8. [PubMed: 20007774] [MGI Ref ID J:156489]
Maatouk DM; Loveland KL; McManus MT; Moore K; Harfe BD. 2008. Dicer1 is required for differentiation of the mouse male germline. Biol Reprod 79(4):696-703. [PubMed: 18633141] [MGI Ref ID J:140872]
Mattiske DM; Han L; Mann JR. 2009. Meiotic maturation failure induced by DICER1 deficiency is derived from primary oocyte ooplasm. Reproduction 137(4):625-32. [PubMed: 19129368] [MGI Ref ID J:150221]
Moreno SG; Attali M; Allemand I; Messiaen S; Fouchet P; Coffigny H; Romeo PH; Habert R. 2010. TGFbeta signaling in male germ cells regulates gonocyte quiescence and fertility in mice. Dev Biol 342(1):74-84. [PubMed: 20346356] [MGI Ref ID J:160474]
Pan J; Eckardt S; Leu NA; Buffone MG; Zhou J; Gerton GL; McLaughlin KJ; Wang PJ. 2009. Inactivation of Nxf2 causes defects in male meiosis and age-dependent depletion of spermatogonia. Dev Biol 330(1):167-74. [PubMed: 19345203] [MGI Ref ID J:149228]
Pelka GJ; Watson CM; Radziewic T; Hayward M; Lahooti H; Christodoulou J; Tam PP. 2006. Mecp2 deficiency is associated with learning and cognitive deficits and altered gene activity in the hippocampal region of mice. Brain 129(Pt 4):887-98. [PubMed: 16467389] [MGI Ref ID J:137513]
Qiu MR; Jiang L; Matthaei KI; Schoenwaelder SM; Kuffner T; Mangin P; Joseph JE; Low J; Connor D; Valenzuela SM; Curmi PM; Brown LJ; Mahaut-Smith M; Jackson SP; Breit SN. 2010. Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene. Genesis 48(2):127-36. [PubMed: 20049953] [MGI Ref ID J:158394]
Quah BJ; Barlow VP; McPhun V; Matthaei KI; Hulett MD; Parish CR. 2008. Bystander B cells rapidly acquire antigen receptors from activated B cells by membrane transfer. Proc Natl Acad Sci U S A 105(11):4259-64. [PubMed: 18337504] [MGI Ref ID J:173514]
Schnabel D; Ramirez L; Gertsenstein M; Nagy A; Lomeli H. 2005. Ectopic expression of Kit(D814Y) in spermatids of transgenic mice, interferes with sperm morphogenesis. Dev Dyn 233(1):29-40. [PubMed: 15736269] [MGI Ref ID J:97480]
Tachibana M; Nozaki M; Takeda N; Shinkai Y. 2007. Functional dynamics of H3K9 methylation during meiotic prophase progression. EMBO J 26(14):3346-59. [PubMed: 17599069] [MGI Ref ID J:123269]
Tang H; Ross A; Capel B. 2008. Expression and functional analysis of Gm114, a putative mammalian ortholog of Drosophila bam. Dev Biol 318(1):73-81. [PubMed: 18423593] [MGI Ref ID J:136802]
Thomsen N; Chappell A; Ali RG; Jones T; Adams DH; Matthaei KI; Campbell HD; Cowin AJ; Arkell RM. 2011. Mouse strains for the ubiquitous or conditional overexpression of the Flii gene Genesis 49:681-688. [PubMed: 21786402] [MGI Ref ID J:168970]
Wai T; Ao A; Zhang X; Cyr D; Dufort D; Shoubridge EA. 2010. The role of mitochondrial DNA copy number in mammalian fertility. Biol Reprod 83(1):52-62. [PubMed: 20130269] [MGI Ref ID J:161982]
Weber S; Eckert D; Nettersheim D; Gillis AJ; Schafer S; Kuckenberg P; Ehlermann J; Werling U; Biermann K; Looijenga LH; Schorle H. 2010. Critical function of AP-2 gamma/TCFAP2C in mouse embryonic germ cell maintenance. Biol Reprod 82(1):214-23. [PubMed: 19776388] [MGI Ref ID J:156642]
Yamaguchi S; Kurimoto K; Yabuta Y; Sasaki H; Nakatsuji N; Saitou M; Tada T. 2009. Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells. Development 136(23):4011-20. [PubMed: 19906868] [MGI Ref ID J:154977]
Yang T; Riehl J; Esteve E; Matthaei KI; Goth S; Allen PD; Pessah IN; Lopez JR. 2006. Pharmacologic and functional characterization of malignant hyperthermia in the R163C RyR1 knock-in mouse. Anesthesiology 105(6):1164-75. [PubMed: 17122579] [MGI Ref ID J:168222]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes are not viable. Mating System +/+ sibling x Heterozygote (Female x Male) 20-JAN-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Heterozygous for Alpltm1(cre)Nagy
Pairs /Price (US dollars $) Pair Genotype $321.00 Heterozygous for Alpltm1(cre)Nagy x Wild-type for Alpltm1(cre)Nagy $321.00 Wild-type for Alpltm1(cre)Nagy x Heterozygous for Alpltm1(cre)Nagy Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Heterozygous for Alpltm1(cre)Nagy
Pairs /Price (US dollars $) Pair Genotype $417.30 Heterozygous for Alpltm1(cre)Nagy x Wild-type for Alpltm1(cre)Nagy $417.30 Wild-type for Alpltm1(cre)Nagy x Heterozygous for Alpltm1(cre)Nagy Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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