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| Homozygous targeted mutant mice have thymocytes and splenocytes that, studied in vitro, are insensitive to pH-dependent cAMP production. A preliminary examination of homozygous animals has not revealed any major abnormalities in the development of the immune system or in its major functions. A green fluorescent protein (GFP) insertion acts as a marker for cells that normally express the targeted gene. This strain may be helpful in elucidating the role of this and other G protein-coupled receptors in the regulation of immune cell responses in acidic microenvironments. | |||||||||||||||
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Owen Witte, University of California, Los Angeles Description
Homozygous targeted mutant mice have thymocytes and splenocytes that, studied in vitro, are insensitive to pH-dependent cAMP production. Despite significant in vitro defects in the acidic pH-induced cAMP production, a preliminary examination of homozygous animals has not revealed any major abnormalities in the development of the immune system or in its major functions. A green fluorescent protein (GFP) insertion acts as a marker for cells that normally express the gene. This strain may be helpful in elucidating the role of this and other G protein-coupled receptors in the regulation of immune cell responses in acidic microenvironments.Development
A targeting vector was designed to replace exon 2 with IRES-GFP and a PGK-neo cassette flanked by loxP sites. The targeting vector was electroporated into 129X1/SvJ embryonic stem (ES) cells. Clones were injected into C57BL/6 blastocysts. Chimeric animals were backcrossed to C57BL/6 for seven generations.
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Phenotype
Phenotype Information
assigned by genotype
Gpr65tm1Witt/Gpr65tm1Witt
B6.Cg-Gpr65tm1Witt
- immune system phenotype
- abnormal immune cell physiology (MGI Ref ID J:96112)
- production of cAMP by total splenocytes was insensitive to pH
- abnormal leukocyte physiology (MGI Ref ID J:96112)
- no pH-dependent cAMP production in thymocytes
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gpr65tm1Witt/Gpr65tm1Witt
C.Cg-Gpr65tm1Witt
- immune system phenotype
- abnormal leukocyte physiology (MGI Ref ID J:96112)
- no pH-dependent cAMP production in thymocytes
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gpr65tm1Witt | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Owen N Witte | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | TDAG8-; | ||
| Mutation Made By | Owen Witte, University of California, Los Angeles | ||
| Strain of Origin | 129X1/SvJ | ||
| Gene Symbol and Name | Gpr65, G-protein coupled receptor 65 | ||
| Chromosome | 12 | ||
| Gene Common Name(s) | Dig1; G-protein coupled receptor 25; Gpcr25; TDAG8; hTDAG8; | ||
| Molecular Note | Most of exon 2 was replaced with an IRES-EGFP and a floxed neo. The absence of transcript expression was confirmed by RT-PCR on spleen and thymus extracts. [MGI Ref ID J:144144] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gpr65tm1Witt, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Radu CG; Nijagal A; McLaughlin J; Wang L; Witte ON. 2005. Differential proton sensitivity of related G protein-coupled receptors T cell death-associated gene 8 and G2A expressed in immune cells. Proc Natl Acad Sci U S A 102(5):1632-7. [PubMed: 15665078] [MGI Ref ID J:96112]
Additional References
Gpr65tm1Witt relatedRadu CG; Cheng D; Nijagal A; Riedinger M; McLaughlin J; Yang LV; Johnson J; Witte ON. 2006. Normal immune development and glucocorticoid-induced thymocyte apoptosis in mice deficient for the T-cell death-associated gene 8 receptor. Mol Cell Biol 26(2):668-77. [PubMed: 16382156] [MGI Ref ID J:144144]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, heterozyogotes or homozygotes may be bred.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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