| |||||||||||||||
| These transgenic mice express the mouse Abca1 (ATP-binding cassette, sub-family A (ABC1), member 1) gene under the control of the mouse Prnp (prion protein) promoter. These founder line E mice have a 6-fold increase in expression of ABCA1 in the cortex over wild-type levels. Transgenic mice exhibit reduced apoE levels in the hippocampus and cerebrospinal fluid (CSF). These mice may be useful in studies of the underlying mechanism of amyloid deposition in the brain and Alzheimers disease. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Hemizygote x +/+ sibling (Female x Male) 23-JAN-09 Species laboratory mouse Generation N5+ (22-JAN-09) Donating Investigator David Holtzman, Washington University Description
These transgenic mice express the mouse Abca1 (ATP-binding cassette, sub-family A (ABC1), member 1) gene under the control of the mouse Prnp (prion protein) promoter.These founder line E mice have a 6-fold increase in expression of ABCA1 in the cortex over wild-type levels. Trangene expression is high in total brain tissue, kidney, testis and muscle as detected by Western blot analysis. Transgenic mice exhibit reduced apoE levels: 40% of wild-type levels in the hippocampus, approximately half of wild-type levels in cerebrospinal fluid (CSF). The apoE protein that is overexpressed has altered biochemical properties and is not as soluble as that found in controls. Lipoprotein particles from the CSF that contain apoE protein are larger in size than wild-type, indicating that the transgenic apoE particles are more lipidated.
Male transgenic mice have atropied testes, defective spermatogenesis and are infertile. The strain can be maintained by mating hemizygous females to wild-type males.
These mice may be useful in studies of the underlying mechanism of amyloid deposition in the brain and Alzheimers disease.
Development
A transgenic construct containing the mouse Abca1 (ATP-binding cassette, sub-family A (ABC1), member 1) gene under the control of the mouse Prnp (prion protein) promoter was injected into fertilized B6CBAF1 eggs. Founder line E was subsequently established. The mice were backcrossed to C57BL/6 for four generations. After arriving at The Jackson Laboratory, the mice were backcrossed to C57BL/6J for one generation.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Abca1
003897 DBA/1-Abca1tm1Jdm/J View Strains carrying other alleles of Abca1 (1 strain)
Additional Web Information
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Prnp-Abca1)EHol/0
involves: C57BL/6 * CBA
- reproductive system phenotype
- abnormal spermatogenesis (MGI Ref ID J:131400)
- elongated spermatids and spermatozoa are absent in testes of mutant males
- abnormal testis morphology (MGI Ref ID J:131400)
- 3-fold increased numbers of apoptotic cells in testes relative to controls
- abnormal testis development (MGI Ref ID J:131400)
- testes exhibit maturation arrest due to absence of mature products of spermatogenesis
- decreased testis weight (MGI Ref ID J:131400)
- 50% reduction in testes mass relative to wild-type controls
- seminiferous tubule degeneration (MGI Ref ID J:131400)
- tubules display atrophy with marked degenerative changes such as large increase in multinucleated giant cells and cells with condensed nuclei
- testicular atrophy (MGI Ref ID J:131400)
- severe atrophy
- male infertility (MGI Ref ID J:131400)
- nervous system phenotype
- abnormal glial cell physiology (MGI Ref ID J:131400)
- Apoe-containing lipoprotein particles secreted by astrocytes contain more lipid than those secreted by non-transgenic controls; lipoprotein fractions contain a greater ratio of cholesterol to Apoe
- amyloid beta deposits (MGI Ref ID J:131400)
- in 12-month old mice have frequent amyloid beta (Abeta) deposits in cortex and hippocampus including molecular layer of hippocampus
- almost no deposits are observed in hilus of dentate gyrus of hippocampus
- thioflavin-S positive fibrillar Abeta plaques are frequently observed in cortex and hippocampus at 12 months
- thioflavin-S positive plaques are associated with reactive microglia
- other phenotype
- amyloid beta deposits (MGI Ref ID J:131400)
- in 12-month old mice have frequent amyloid beta (Abeta) deposits in cortex and hippocampus including molecular layer of hippocampus
- almost no deposits are observed in hilus of dentate gyrus of hippocampus
- thioflavin-S positive fibrillar Abeta plaques are frequently observed in cortex and hippocampus at 12 months
- thioflavin-S positive plaques are associated with reactive microglia
- endocrine/exocrine gland phenotype
- abnormal testis morphology (MGI Ref ID J:131400)
- 3-fold increased numbers of apoptotic cells in testes relative to controls
- abnormal testis development (MGI Ref ID J:131400)
- testes exhibit maturation arrest due to absence of mature products of spermatogenesis
- decreased testis weight (MGI Ref ID J:131400)
- 50% reduction in testes mass relative to wild-type controls
- seminiferous tubule degeneration (MGI Ref ID J:131400)
- tubules display atrophy with marked degenerative changes such as large increase in multinucleated giant cells and cells with condensed nuclei
- testicular atrophy (MGI Ref ID J:131400)
- severe atrophy
- homeostasis/metabolism phenotype
- *normal* homeostasis/metabolism phenotype (MGI Ref ID J:131400)
- plasma levels of cholesterol including HDL-associated cholesterol and phospolipids, and Apoe are not significantly different from controls at 3 months
- abnormal protein level (MGI Ref ID J:131400)
- mice show 40% reduction in Apoe levels in hippocampus at 3 months; in CSF, levels of Apoe are decreased >40%
- brain lysates contain significantly lower levels of Clusterin than control samples
This mouse can be used to support research in many areas including:
Neurobiology Research
Alzheimer's Disease
Reproductive Biology Research
Fertility Defects
males only
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Prnp-Abca1)EHol | ||
|---|---|---|---|
| Allele Name | transgene insertion E, David M Holtzman | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | PrP-mAbca1 line E; | ||
| Mutation Made By | David Holtzman, Washington University | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Expressed Gene | Abca1, ATP-binding cassette, sub-family A (ABC1), member 1, mouse, laboratory | ||
| Promoter | Prnp, prion protein, mouse, laboratory | ||
| Molecular Note | A transgenic construct containing the mouse Abca1 (ATP-binding cassette, sub-family A (ABC1), member 1) gene under the control of the mouse Prnp (prion protein) promoter was injected into fertilized B6CBAF1 eggs. Founder line E was subsequently established. These founder line E mice have a 6-fold increase in expression of ABCA1 in the cortex over wildtype levels. Transgene expression is high in total brain tissue, kidney, testis and muscle as detected by Western blot analysis. [MGI Ref ID J:131400] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Prnp-Abca1), Standard PCR
Tg(Prnp-Abca1), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Wahrle SE; Jiang H; Parsadanian M; Kim J; Li A; Knoten A; Jain S; Hirsch-Reinshagen V; Wellington CL; Bales KR; Paul SM; Holtzman DM. 2008. Overexpression of ABCA1 reduces amyloid deposition in the PDAPP mouse model of Alzheimer disease. J Clin Invest 118(2):671-82. [PubMed: 18202749] [MGI Ref ID J:131400]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375/MGL377
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these transgenic mice can be bred by mating female hemizygote to a wildtype male. Male transgenic mice are sterile. Mating System Hemizygote x +/+ sibling (Female x Male) 23-JAN-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Prnp-Abca1)EHol
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Prnp-Abca1)EHol x Noncarrier
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Prnp-Abca1)EHol
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Prnp-Abca1)EHol x Noncarrier
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.