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| TH-Cre transgenic mice have the rat tyrosine hydroxylase (TH) promoter directing expression of Cre recombinase to catecholaminergic cells, and may be useful for generating conditional mutations in these cells for studying dopaminergic cell function, Parkinson's disease, and other neurodegenerative disorders. | |||||||||||||||
- Description
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- Genes & alleles
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Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 09-JUN-09 Species laboratory mouse Generation N6+F5 (13-SEP-11)
Generation DefinitionsDonating Investigator Ted Dawson, Johns Hopkins Univ School of Medicine Description
Mice hemizygous for the TH-Cre transgene are viable and fertile, with the rat tyrosine hydroxylase (TH) promoter directing expression of Cre recombinase to catecholaminergic cells. Using crosses to reporter strains, cre activity is confirmed in catecholaminergic cells and is present in many of the projection areas of these neuronal populations. A mosaic of cre activity is noted in TH-positive neurons. Several other areas that are not typically thought to have active TH expression, including the lateral septal nucleus, accessory olfactory bulb, suparafascicular thalamus, and pretectal area, also exhibit Cre recombinase activity (possibly as a result of TH promoter activity in precursor cell populations or ectopic expression from the exogenous TH promoter). Some TH-negative cells closely clustered around and within TH-positive nuclei demonstrate cre activity. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These TH-Cre transgenic mice are a tool for generating conditional mutations in catecholaminergic cells and may be useful for studying dopaminergic cell function, Parkinson's disease, and other neurodegenerative disorders.Development
The TH-Cre transgene was designed with a 9.0 kb fragment of the rat tyrosine hydroxylase (TH) promoter, synthetic 5' UTR intron, cre cDNA, and SV40 late region polyadenylation site. The transgene was microinjected into the pronuclei of fertilized B6/SJLF2 oocytes. Chimeric mice were bred to C57BL/6NCrl to confirm germline transmission and establish founder line 1 (TH-Cre 1). The resulting TH-Cre 1 transgenic mice were subsequently maintained by backcrossing to C57BL/6NCrl mice (see SNP note below) for greater than 5 generations prior to arrival at The Jackson Laboratory Repository. Upon arrival, transgenic mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) to establish the colony.A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 005304 C57BL/6NJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Parkinson's Disease Models
View Parkinson's Disease Models (99 strains)
012588 B6.Cg-Tg(TH-ALPP)1Erav/J 008750 B6.Cg-Tg(Th-Oprm1)4Jtw/J 008245 C57BL/6J-Tg(Th-SNCA)5Eric/J 008239 C57BL/6J-Tg(Th-SNCA*A30P*A53T)39Eric/J
Additional Web Information
Introduction to Cre-lox technology
Visit the Parkinson's Disease Resource site for helpful information on Parkinson's and research resources.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Parkinson's Disease
strains expressing cre
Research Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Th-cre)1Tmd | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Ted M Dawson | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | TH-cre; | ||
| Mutation Made By | Ted Dawson, Johns Hopkins Univ School of Medicine | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | dopaminergic neurons | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Th, tyrosine hydroxylase, rat | ||
| Driver Note | Th | ||
| Molecular Note | Cre recombinase is expressed under the control of the rat tyrosine hydroxylase promoter. Cre recombinase activity is detected in catecholaminergic cells. This is a representative record representing TH-cre1 - TH-cre5 all of which have identical activity.The authors state that TH-cre4 appears to be X-linked. [MGI Ref ID J:99848] | ||
| Gene Symbol and Name | Tg(Th-cre)1Tmd, transgene insertion 1, Ted M Dawson | ||
| Chromosome | UN | ||
| Gene Common Name(s) | TH-cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Savitt JM; Jang SS; Mu W; Dawson VL; Dawson TM. 2005. Bcl-x is required for proper development of the mouse substantia nigra. J Neurosci 25(29):6721-8. [PubMed: 16033881] [MGI Ref ID J:99848]
Additional References
Daher JP; Ying M; Banerjee R; McDonald RS; Dumas Hahn M; Yang L; Beal MF; Thomas B; Dawson VL; Dawson TM; Moore DJ. 2009. Conditional transgenic mice expressing C-terminally truncated human alpha-synuclein (alphaSyn119) exhibit reduced striatal dopamine without loss of nigrostriatal pathway dopaminergic neurons. Mol Neurodegener 4(1):34. [PubMed: 19630976] [MGI Ref ID J:150777]
Tg(Th-cre)1Tmd relatedChuang JC; Perello M; Sakata I; Osborne-Lawrence S; Savitt JM; Lutter M; Zigman JM. 2011. Ghrelin mediates stress-induced food-reward behavior in mice. J Clin Invest 121(7):2684-92. [PubMed: 21701068] [MGI Ref ID J:175916]
Daher JP; Ying M; Banerjee R; McDonald RS; Dumas Hahn M; Yang L; Beal MF; Thomas B; Dawson VL; Dawson TM; Moore DJ. 2009. Conditional transgenic mice expressing C-terminally truncated human alpha-synuclein (alphaSyn119) exhibit reduced striatal dopamine without loss of nigrostriatal pathway dopaminergic neurons. Mol Neurodegener 4(1):34. [PubMed: 19630976] [MGI Ref ID J:150777]
Fuerst PG; Bruce F; Rounds RP; Erskine L; Burgess RW. 2012. Cell autonomy of DSCAM function in retinal development. Dev Biol 361(2):326-37. [PubMed: 22063212] [MGI Ref ID J:179393]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to C57BL/6NJ (Stock No. 005304) inbred mice. The donating investigator reports that homozygous mice are viable. Mating System Noncarrier x Hemizygote (Female x Male) 09-JUN-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(Th-cre)1Tmd
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(Th-cre)1Tmd x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(Th-cre)1Tmd Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(Th-cre)1Tmd
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(Th-cre)1Tmd x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(Th-cre)1Tmd Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 005304 C57BL/6NJ | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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