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| These IRG transgenic mice are a double-fluorescent, Cre-reporter strain; with widespread expression of red fluorescence prior to Cre recombinase exposure, and green fluorescence following cre-mediated recombination in a pattern determined by cre expression, and should provide a versatile tool for analyzing complex cellular relationships in a wide variety of tissues. | |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Donating Investigator Gregory Elder, James J. Peters VA Medical Center Description
Hemizygous IRG transgenic mice are viable and fertile, with widespread expression of a loxP-flanked optimized red fluorescent protein variant (DsRed-Express) directed to embryonic and adult tissues by the CAG promoter prior to exposure to Cre recombinase. When bred to cre-expressing mice, the resulting offspring have the DsRed-Express cassette deleted in the cre-expressing tissue(s), allowing expression of the enhanced green fluorescent protein (EGFP) cassette located just downstream. These IRG transgenic mice are a double-fluorescent, Cre-reporter strain; with widespread expression of red fluorescence prior to Cre recombinase exposure, and green fluorescence following cre-mediated recombination in a pattern determined by cre expression, and should provide a versatile tool for analyzing complex cellular relationships in a wide variety of tissues.For example, when using IRG transgenic mice along with Nestin-Cre mice (see Stock No. 003771), red and green native fluorescence can be imaged simultaneously in thick tissue sections by confocal microscopy; thus allowing for analysis of complex reconstructions suitable for studying neuronal morphologies as well as neurovascular interactions in brain.
Development
The CCAG-R/G expression vector was designed with (from 5' to 3') a Human cytomegalovirus immediate early enhancer and chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP site, a DsRed-Express protein sequence followed by three SV40 polyadenylation signals (DsRed-Express (Clontech) is a red fluorescent protein tetramer with improved solubility and fluorescence detection), a second loxP site, an enhanced green fluorescent protein (EGFP) sequence itself followed by a rabbit beta-globin polyadenylation signal. To minimize integration site effects and possible promoter interference, the CCAG-R/S expression vector was flanked on both sides with two paired copies of anti-sense oriented 5'-HS4 insulator sequences from the chicken beta-globin gene; generating the final "insulator/red/green" targeting vector (pIRG). The donating investigator confirms that this 9.4 kb transgene was injected into fertilized oocytes from B6C3F1 hybrid mice. Founder mice were bred to C57BL/6 wild-type mice to generate IRG line 5 (estimated to contain seven copies of the transgene). These IRG mice were maintained on a mixed C57BL/6 and C3H genetic background by breeding hemizygotes with C57BL/6 or B6C3 hybrid mice for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100010 B6C3F1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (169 strains)
006051 B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J 006872 B6.Cg-Tg(Ins1-DsRed*T4)32Hara/J 005328 NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J 005438 STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J 005441 STOCK Tg(CAG-DsRed*MST)1Nagy/J 008241 STOCK Tg(Cspg4-DsRed.T1)1Akik/J 006866 STOCK Tg(Ins1-DsRed*T4)32Hara/J
Additional Web Information
Cre-lox Systems
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(CAG-DsRed,-EGFP)5Gae/0
involves: C3H * C57BL/6
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:137251)
- hemizygous mice are viable and fertile; no detailed phenotypic is provided
This mouse can be used to support research in many areas including:
DsRed relatedNeurobiology Research
Cre-lox System (loxP-flanked Sequences: Test/Reporter)
Fluorescent protein expression in neural tissue
Research Tools
Cre-lox System (loxP-flanked Sequences: Test/Reporter)
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Genetics Research (Tissue/Cell Markers: multiple)
GFP relatedResearch Tools
Fluorescent Proteins
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-DsRed,-EGFP)5Gae | ||
|---|---|---|---|
| Allele Name | transgene insertion 5, Gregory A Elder | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | IRG; insulator/red/green; | ||
| Mutation Made By | Gregory Elder, James J. Peters VA Medical Center | ||
| Strain of Origin | (C57BL/6 x C3H)F1 | ||
| Site of Expression | Widespread expression of red fluorescent protein is observed; following cre-mediated recombination, GFP is expressed. | ||
| Expressed Gene | DsRed, red fluorescent protein, | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | The CCAG-R/G expression vector was designed with (from 5' to 3') a Human cytomegalovirus immediate early enhancer and chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP site, a DsRed-Express protein sequence followed by three SV40 polyadenylation signals (DsRed-Express (Clontech) is a red fluorescent protein tetramer with improved solubility and fluorescence detection), a second loxP site, an enhanced green fluorescent protein (EGFP) sequence itself followed by a rabbit beta-globin polyadenylation signal. To minimize integration site effects and possible promoter interference, the CCAG-R/S expression vector was flanked on both sides with two paired copies of anti-sense oriented 5'-HS4 insulator sequences from the chicken beta-globin gene; generating the final "insulator/red/green" targeting vector (pIRG). Line 5 was established and has an estimated transgene copy number of 7. Embryonic and adult tissues exhibit widespread red fluorescent protein expression in the absence of cre recombinase. [MGI Ref ID J:137251] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
De Gasperi R; Rocher AB; Sosa MA; Wearne SL; Perez GM; Friedrich VL Jr; Hof PR; Elder GA. 2008. The IRG mouse: a two-color fluorescent reporter for assessing Cre-mediated recombination and imaging complex cellular relationships in situ. Genesis 46(6):308-17. [PubMed: 18543298] [MGI Ref ID J:137251]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred with noncarrier (wildtype) mice.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100010 B6C3F1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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Contact information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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