Strain Name:

B6.Cg-Dmc1tm1Jcs/JcsJ

Stock Number:

008608

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Availability:

Cryopreserved - Ready for recovery

These mice have a targeted "knockout" mutation of the Dmc1 gene (DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)) and may be useful in studying gametogenesis and meiosis, including double strand breaks, chromosome asynapsis, DNA repair mechanisms, homologous recombination, and the pachytene checkpoint.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Generation?pN1
Generation Definitions
 
Donating Investigator John Schimenti,   Cornell University

Description
While mice heterozygous for this meiosis-specific RecA homolog (Dmc1) mutation are viable and fertile, homozygotes are viable but sterile due to arrest of gametogenesis in the first meiotic prophase (prophase I). No RNA message from the targeted gene is observed in homozygous testis. Homozygous males have reduced testis size and no mature spermatozoa in the epididymis; spermatocytes undergo meiotic arrest from defective double strand break repair and extensive chromosome asynapsis. Homozygous females exhibit defective oogenesis due to similar aberrations occurring at the pachytene stage or earlier and thus have small, malformed ovaries with complete depletion of oocytes and follicles by adulthood. These Dmc1-mutant mice may be useful in studying gametogenesis and meiosis (including double strand breaks, chromosome asynapsis, DNA repair mechanisms, homologous recombination, and the pachytene checkpoint).

Development
A targeting vector was designed to replace a region of the targeted gene containing the RecA-like DNA binding motif (GEFRTGKT) with a neomycin resistance cassette. This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6J females. Mutant mice were backcrossed to C57BL/6J for at least 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Dmc1tm1Jcs/Dmc1tm1Jcs

        involves: 129S4/SvJae * C57BL/6J
  • reproductive system phenotype
  • abnormal reproductive system morphology   (MGI Ref ID J:47308)
    • abnormal gametogenesis   (MGI Ref ID J:47308)
      • abnormal oogenesis   (MGI Ref ID J:47308)
        • abnormal female meiosis
          • defects during prophase of meiosis I beginning at, or prior to, the pachytene stage   (MGI Ref ID J:47308)
        • absent oocytes   (MGI Ref ID J:183982)
      • abnormal spermatogenesis   (MGI Ref ID J:47308)
        • arrest of male meiosis
          • arrest during prophase of meiosis I at a zygotene-like stage in which chromosomal synapsis was not initiated   (MGI Ref ID J:47308)
        • azoospermia   (MGI Ref ID J:47308)
    • absent ovarian follicles   (MGI Ref ID J:47308)
    • decreased ovary weight
      • approximately one-third the weight of those of controls   (MGI Ref ID J:47308)
    • decreased testis weight
      • approximately one-third the weight of those of controls   (MGI Ref ID J:47308)
  • infertility   (MGI Ref ID J:47308)
    • female infertility   (MGI Ref ID J:47308)
    • male infertility   (MGI Ref ID J:47308)
  • endocrine/exocrine gland phenotype
  • absent ovarian follicles   (MGI Ref ID J:47308)
  • decreased ovary weight
    • approximately one-third the weight of those of controls   (MGI Ref ID J:47308)
  • decreased testis weight
    • approximately one-third the weight of those of controls   (MGI Ref ID J:47308)

Dmc1tm1Jcs/Dmc1tm1Jcs

        involves: 129S4/SvJae * C57BL/6
  • reproductive system phenotype
  • abnormal male meiosis
    • impaired double strand break processing and loss of preleptotene pairing   (MGI Ref ID J:194067)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Other
      DNA Repair

Cell Biology Research
DNA Damage Response

Developmental Biology Research
Internal/Organ Defects
      gonads

Endocrine Deficiency Research
Gonad Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads
      germ cell deficient
Endocrine Deficiencies Affecting Gonads
Fertility Defects

Research Tools
Reproductive Biology Research
      male germ cells
      oocyte

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Dmc1tm1Jcs
Allele Name targeted mutation 1, John C Schimenti
Allele Type Targeted (knock-out)
Common Name(s) Dmc1-;
Mutation Made By John Schimenti,   Cornell University
Strain of Origin(C57BL/6 x 129S4/SvJae)F1
ES Cell Line Namev6.5
ES Cell Line Strain(C57BL/6 x 129S4/SvJae)F1
Gene Symbol and Name Dmc1, DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination
Chromosome 15
Gene Common Name(s) DMC1H; Dmc1h; LIM15; Mei11; dJ199H16.1; disrupted meiotic cDNA homolog 1; meiosis defective 11; sgdp; spermatogonial depletion;
Molecular Note A neomycin selection cassette replaced a genomic fragment containing sequences encoding the conserved DNA binding motif required for protein activity. RT-PCR analysis on RNA derived from testis of homozygous males demonstrated that no detectable transcript was produced from this allele. [MGI Ref ID J:47308]

Genotyping

Genotyping Information

Genotyping Protocols

Dmc1tm1Jcs, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Pittman DL; Cobb J; Schimenti KJ; Wilson LA; Cooper DM; Brignull E ; Handel MA ; Schimenti JC. 1998. Meiotic prophase arrest with failure of chromosome synapsis in mice deficient for Dmc1, a germline-specific RecA homolog. Mol Cell 1(5):697-705. [PubMed: 9660953]  [MGI Ref ID J:47308]

Additional References

Dmc1tm1Jcs related

Bannister LA; Pezza RJ; Donaldson JR; de Rooij DG; Schimenti KJ; Camerini-Otero RD; Schimenti JC. 2007. A dominant, recombination-defective allele of Dmc1 causing male-specific sterility. PLoS Biol 5(5):e105. [PubMed: 17425408]  [MGI Ref ID J:122037]

Barchi M; Mahadevaiah S; Di Giacomo M; Baudat F; de Rooij DG; Burgoyne PS; Jasin M; Keeney S. 2005. Surveillance of different recombination defects in mouse spermatocytes yields distinct responses despite elimination at an identical developmental stage. Mol Cell Biol 25(16):7203-15. [PubMed: 16055729]  [MGI Ref ID J:133053]

Boateng KA; Bellani MA; Gregoretti IV; Pratto F; Camerini-Otero RD. 2013. Homologous pairing preceding SPO11-mediated double-strand breaks in mice. Dev Cell 24(2):196-205. [PubMed: 23318132]  [MGI Ref ID J:194067]

Bolcun-Filas E; Rinaldi VD; White ME; Schimenti JC. 2014. Reversal of female infertility by Chk2 ablation reveals the oocyte DNA damage checkpoint pathway. Science 343(6170):533-6. [PubMed: 24482479]  [MGI Ref ID J:207474]

Daniel K; Lange J; Hached K; Fu J; Anastassiadis K; Roig I; Cooke HJ; Stewart AF; Wassmann K; Jasin M; Keeney S; Toth A. 2011. Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1. Nat Cell Biol 13(5):599-610. [PubMed: 21478856]  [MGI Ref ID J:173089]

Di Giacomo M; Barchi M; Baudat F; Edelmann W; Keeney S; Jasin M. 2005. Distinct DNA-damage-dependent and -independent responses drive the loss of oocytes in recombination-defective mouse mutants. Proc Natl Acad Sci U S A 102(3):737-42. [PubMed: 15640358]  [MGI Ref ID J:96250]

Lange J; Pan J; Cole F; Thelen MP; Jasin M; Keeney S. 2011. ATM controls meiotic double-strand-break formation. Nature 479(7372):237-40. [PubMed: 22002603]  [MGI Ref ID J:178306]

Li X; Schimenti JC. 2007. Mouse Pachytene Checkpoint 2 (Trip13) Is Required for Completing Meiotic Recombination but Not Synapsis. PLoS Genet 3(8):e130. [PubMed: 17696610]  [MGI Ref ID J:123677]

Liebe B; Petukhova G; Barchi M; Bellani M; Braselmann H; Nakano T; Pandita TK; Jasin M; Fornace A; Meistrich ML; Baarends WM; Schimenti J; de Lange T; Keeney S; Camerini-Otero RD; Scherthan H. 2006. Mutations that affect meiosis in male mice influence the dynamics of the mid-preleptotene and bouquet stages. Exp Cell Res 312(19):3768-81. [PubMed: 17010969]  [MGI Ref ID J:147391]

Lyndaker AM; Lim PX; Mleczko JM; Diggins CE; Holloway JK; Holmes RJ; Kan R; Schlafer DH; Freire R; Cohen PE; Weiss RS. 2013. Conditional inactivation of the DNA damage response gene Hus1 in mouse testis reveals separable roles for components of the RAD9-RAD1-HUS1 complex in meiotic chromosome maintenance. PLoS Genet 9(2):e1003320. [PubMed: 23468651]  [MGI Ref ID J:195191]

Mahadevaiah SK; Bourc'his D; de Rooij DG; Bestor TH; Turner JM; Burgoyne PS. 2008. Extensive meiotic asynapsis in mice antagonises meiotic silencing of unsynapsed chromatin and consequently disrupts meiotic sex chromosome inactivation. J Cell Biol 182(2):263-76. [PubMed: 18663141]  [MGI Ref ID J:139518]

Reinholdt LG; Schimenti JC. 2005. Mei1 is epistatic to Dmc1 during mouse meiosis. Chromosoma 114(2):127-34. [PubMed: 15928951]  [MGI Ref ID J:103876]

Shin YH; McGuire MM; Rajkovic A. 2013. Mouse HORMAD1 is a meiosis i checkpoint protein that modulates DNA double- strand break repair during female meiosis. Biol Reprod 89(2):29. [PubMed: 23759310]  [MGI Ref ID J:203746]

Souquet B; Abby E; Herve R; Finsterbusch F; Tourpin S; Le Bouffant R; Duquenne C; Messiaen S; Martini E; Bernardino-Sgherri J; Toth A; Habert R; Livera G. 2013. MEIOB targets single-strand DNA and is necessary for meiotic recombination. PLoS Genet 9(9):e1003784. [PubMed: 24068956]  [MGI Ref ID J:203426]

Wojtasz L; Cloutier JM; Baumann M; Daniel K; Varga J; Fu J; Anastassiadis K; Stewart AF; Remenyi A; Turner JM; Toth A. 2012. Meiotic DNA double-strand breaks and chromosome asynapsis in mice are monitored by distinct HORMAD2-independent and -dependent mechanisms. Genes Dev 26(9):958-73. [PubMed: 22549958]  [MGI Ref ID J:183982]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous mice may be bred together (or to wildtype siblings or C57BL/6J inbred mice). Homozygous mice are viable but sterile.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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