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| Mice harboring the Vav-iCre transgene have the mouse HS21/45 control regions directing expression of an optimized variant of Cre recombinase (iCre) to hematopoietic cells (and their progenitors), and may be useful for generating conditional mutations in hematopoietic cells. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
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Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 28-APR-09 Species laboratory mouse Generation N16+F6 (14-MAR-11)
Generation DefinitionsDonating Investigator Dimitris Kioussis, MRC Natl Inst for Med Research Description
Mice hemizygous for this Vav-iCre transgene are viable and fertile, with the mouse HS21/45-vav control regions directing expression of an optimized variant of Cre recombinase (iCre) to hematopoietic cells (and their progenitors). Using crosses to a reporter strain, variegated germ line (testis and ovaries), and heart and gut expression is also reported. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Vav-iCre transgenic mice may be useful for generating conditional mutations in hematopoietic cells.Of note, this Vav-iCre strain (Stock No. 008610) allows reliable deletion of specific genes throughout the entire hematopoietic compartment, whereas the hCD2-iCre strain (Stock No. 008520) allows targeting to be focused to T cells and B cells.
Development
The Vav-iCre transgene was designed with the mouse vav 1 oncogene (Vav1) promoter region (containing the 2 proximal upstream DNase-I hypersensitive (HS) sites (HS21)), an optimized variant of Cre recombinase (iCre; improved with mammalian codon usage, removed putative cryptic splce sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals) and an SV40 polyA signal replacing Vav1 exon 1, and Vav1 intron 1 (containing both proximal HS sites (HS45)). The transgene was microinjected into the pronuclei of fertilized oocytes from (CBA/Ca x C57BL/10)F1 mice. Founder mice (founder line ".A2") were established and used to generate mutant mice. The resulting Vav-iCre transgenic mice were subsequently bred to wildtype C57BL/6 mice for more than 16 generations prior to arrival at The Jackson Laboratory Repository. Upon arrival, the Vav-iCre transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Vav1
019379 B6.129S-Vav1tm1Tyb/J 010505 C57BL/6-Tg(Vav1-NUP98/HOXD13)G2Apla/J View Strains carrying other alleles of Vav1 (2 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Hematological Research
Immunology and Inflammation Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Vav1-cre)A2Kio | ||
|---|---|---|---|
| Allele Name | transgene insertion A2, Dimitris Kioussis | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Tg(Vav1-cre)1Kio; Vav-cre; Vav-iCre; Vav1 (HS21/5)-iCre; Vav1-Cre; VavCre; | ||
| Mutation Made By | Dimitris Kioussis, MRC Natl Inst for Med Research | ||
| Strain of Origin | (CBA/Ca x C57BL/10)F2 | ||
| Site of Expression | Cre recombinase is expressed in lymph nodes, thymus, spleen, Peyer's patches, intestinal cryptopatches, ovaries and testes. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Vav1, vav 1 oncogene, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Vav1-cre)A2Kio, transgene insertion A2, Dimitris Kioussis | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(Vav1-cre)1Kio; Vav-cre; Vav-iCre; Vav1-Cre; VavCre; transgene insertion 1, Dimitris Kioussis; | ||
| Driver Note | Vav1 | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a vav promoter. This promoter was shown to be active in lymph nodes, thymus, spleen, Peyer's patches, intestinal cryptopatches, ovaries and testes. [MGI Ref ID J:81568] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic iCre, Melt Curve Analysis
Generic iCre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
De Boer J; Williams A; Skavdis G; Harker N; Coles M; Tolaini M; Norton T; Williams K; Roderick K; Potocnik AJ; Kioussis D. 2003. Transgenic mice with hematopoietic and lymphoid specific expression of Cre. Eur J Immunol 33(2):314-25. [PubMed: 12548562] [MGI Ref ID J:81568]
Ogilvy S; Elefanty AG; Visvader J; Bath ML; Harris AW; Adams JM. 1998. Transcriptional regulation of vav, a gene expressed throughout the hematopoietic compartment. Blood 91(2):419-30. [PubMed: 9427694] [MGI Ref ID J:45594]
Ogilvy S; Metcalf D; Gibson L; Bath ML; Harris AW; Adams JM. 1999. Promoter elements of vav drive transgene expression in vivo throughout the hematopoietic compartment. Blood 94(6):1855-63. [PubMed: 10477714] [MGI Ref ID J:140234]
Shimshek DR; Kim J; Hubner MR; Spergel DJ; Buchholz F; Casanova E; Stewart AF; Seeburg PH; Sprengel R. 2002. Codon-improved Cre recombinase (iCre) expression in the mouse. Genesis 32(1):19-26. [PubMed: 11835670] [MGI Ref ID J:78663]
Additional References
Tg(Vav1-cre)A2Kio relatedArndt PG; Strahan B; Wang Y; Long C; Horiuchi K; Walcheck B. 2011. Leukocyte ADAM17 Regulates Acute Pulmonary Inflammation. PLoS One 6(5):e19938. [PubMed: 21603616] [MGI Ref ID J:172742]
Banerjee A; Grumont R; Gugasyan R; White C; Strasser A; Gerondakis S. 2008. NF-kappaB1 and c-Rel cooperate to promote the survival of TLR4-activated B cells by neutralizing Bim via distinct mechanisms. Blood 112(13):5063-73. [PubMed: 18805964] [MGI Ref ID J:143616]
Berquam-Vrieze KE; Nannapaneni K; Brett BT; Holmfeldt L; Ma J; Zagorodna O; Jenkins NA; Copeland NG; Meyerholz DK; Knudson CM; Mullighan CG; Scheetz TE; Dupuy AJ. 2011. Cell of origin strongly influences genetic selection in a mouse model of T-ALL. Blood 118(17):4646-56. [PubMed: 21828136] [MGI Ref ID J:178407]
Berry R; Rodeheffer MS. 2013. Characterization of the adipocyte cellular lineage in vivo. Nat Cell Biol 15(3):302-8. [PubMed: 23434825] [MGI Ref ID J:195198]
Bertossi A; Aichinger M; Sansonetti P; Lech M; Neff F; Pal M; Wunderlich FT; Anders HJ; Klein L; Schmidt-Supprian M. 2011. Loss of Roquin induces early death and immune deregulation but not autoimmunity. J Exp Med 208(9):1749-56. [PubMed: 21844204] [MGI Ref ID J:177784]
Buza-Vidas N; Duarte S; Luc S; Bouriez-Jones T; Woll PS; Jacobsen SE. 2011. GATA3 is redundant for maintenance and self-renewal of hematopoietic stem cells. Blood 118(5):1291-3. [PubMed: 21670475] [MGI Ref ID J:176922]
Case AJ; McGill JL; Tygrett LT; Shirasawa T; Spitz DR; Waldschmidt TJ; Legge KL; Domann FE. 2011. Elevated mitochondrial superoxide disrupts normal T cell development, impairing adaptive immune responses to an influenza challenge. Free Radic Biol Med 50(3):448-58. [PubMed: 21130157] [MGI Ref ID J:168481]
Cataisson C; Salcedo R; Hakim S; Moffitt BA; Wright L; Yi M; Stephens R; Dai RM; Lyakh L; Schenten D; Yuspa HS; Trinchieri G. 2012. IL-1R-MyD88 signaling in keratinocyte transformation and carcinogenesis. J Exp Med 209(9):1689-702. [PubMed: 22908325] [MGI Ref ID J:191813]
Chamson-Reig A; Arany EJ; Hill DJ. 2010. Lineage tracing and resulting phenotype of haemopoietic-derived cells in the pancreas during beta cell regeneration. Diabetologia 53(10):2188-97. [PubMed: 20585934] [MGI Ref ID J:165691]
Chi MN; Auriol J; Jegou B; Kontoyiannis DL; Turner JM; de Rooij DG; Morello D. 2011. The RNA-binding protein ELAVL1/HuR is essential for mouse spermatogenesis, acting both at meiotic and postmeiotic stages. Mol Biol Cell 22(16):2875-2885. [PubMed: 21737689] [MGI Ref ID J:175695]
Decker T; Pasca di Magliano M; McManus S; Sun Q; Bonifer C; Tagoh H; Busslinger M. 2009. Stepwise activation of enhancer and promoter regions of the B cell commitment gene Pax5 in early lymphopoiesis. Immunity 30(4):508-20. [PubMed: 19345119] [MGI Ref ID J:147974]
Delogu A; Schebesta A; Sun Q; Aschenbrenner K; Perlot T; Busslinger M. 2006. Gene repression by Pax5 in B cells is essential for blood cell homeostasis and is reversed in plasma cells. Immunity 24(3):269-81. [PubMed: 16546096] [MGI Ref ID J:113322]
Ding L; Saunders TL; Enikolopov G; Morrison SJ. 2012. Endothelial and perivascular cells maintain haematopoietic stem cells. Nature 481(7382):457-62. [PubMed: 22281595] [MGI Ref ID J:180431]
Finney BA; Schweighoffer E; Navarro-Nunez L; Benezech C; Barone F; Hughes CE; Langan SA; Lowe KL; Pollitt AY; Mourao-Sa D; Sheardown S; Nash GB; Smithers N; Reis e Sousa C; Tybulewicz VL; Watson SP. 2012. CLEC-2 and Syk in the megakaryocytic/platelet lineage are essential for development. Blood 119(7):1747-56. [PubMed: 22186994] [MGI Ref ID J:181715]
Gerbaulet A; Wickenhauser C; Scholten J; Peschke K; Drube S; Horny HP; Kamradt T; Naumann R; Muller W; Krieg T; Waskow C; Hartmann K; Roers A. 2011. Mast cell hyperplasia, B-cell malignancy, and intestinal inflammation in mice with conditional expression of a constitutively active kit. Blood 117(6):2012-21. [PubMed: 21148330] [MGI Ref ID J:169611]
Hart GT; Wang X; Hogquist KA; Jameson SC. 2011. Kruppel-like factor 2 (KLF2) regulates B-cell reactivity, subset differentiation, and trafficking molecule expression. Proc Natl Acad Sci U S A 108(2):716-21. [PubMed: 21187410] [MGI Ref ID J:168687]
Hazenbos WL; Wu P; Eastham-Anderson J; Kinoshita T; Brown EJ. 2011. Impaired FcepsilonRI stability, signaling, and effector functions in murine mast cells lacking glycosylphosphatidylinositol-anchored proteins. Blood 118(16):4377-83. [PubMed: 21865342] [MGI Ref ID J:178391]
He C; Hu H; Braren R; Fong SY; Trumpp A; Carlson TR; Wang RA. 2008. c-myc in the hematopoietic lineage is crucial for its angiogenic function in the mouse embryo. Development 135(14):2467-77. [PubMed: 18550710] [MGI Ref ID J:137645]
Hou B; Reizis B; Defranco AL. 2008. Toll-like Receptors Activate Innate and Adaptive Immunity by using Dendritic Cell-Intrinsic and -Extrinsic Mechanisms. Immunity 29(2):272-82. [PubMed: 18656388] [MGI Ref ID J:139001]
Ito T; Kwon HY; Zimdahl B; Congdon KL; Blum J; Lento WE; Zhao C; Lagoo A; Gerrard G; Foroni L; Goldman J; Goh H; Kim SH; Kim DW; Chuah C; Oehler VG; Radich JP; Jordan CT; Reya T. 2010. Regulation of myeloid leukaemia by the cell-fate determinant Musashi. Nature 466(7307):765-8. [PubMed: 20639863] [MGI Ref ID J:162770]
Kosan C; Saba I; Godmann M; Herold S; Herkert B; Eilers M; Moroy T. 2010. Transcription factor miz-1 is required to regulate interleukin-7 receptor signaling at early commitment stages of B cell differentiation. Immunity 33(6):917-28. [PubMed: 21167753] [MGI Ref ID J:167721]
Kubovcakova L; Lundberg P; Grisouard J; Hao-Shen H; Romanet V; Andraos R; Murakami M; Dirnhofer S; Wagner KU; Radimerski T; Skoda RC. 2013. Differential effects of hydroxyurea and INC424 on mutant allele burden and myeloproliferative phenotype in a JAK2-V617F polycythemia vera mouse model. Blood 121(7):1188-99. [PubMed: 23264594] [MGI Ref ID J:194600]
Kwon K; Hutter C; Sun Q; Bilic I; Cobaleda C; Malin S; Busslinger M. 2008. Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development. Immunity 28(6):751-62. [PubMed: 18538592] [MGI Ref ID J:137719]
Lai JC; Ponti S; Pan D; Kohler H; Skoda RC; Matthias P; Nagamine Y. 2012. The DEAH-box helicase RHAU is an essential gene and critical for mouse hematopoiesis. Blood 119(18):4291-300. [PubMed: 22422825] [MGI Ref ID J:185179]
Long C; Hosseinkhani MR; Wang Y; Sriramarao P; Walcheck B. 2012. ADAM17 activation in circulating neutrophils following bacterial challenge impairs their recruitment. J Leukoc Biol 92(3):667-72. [PubMed: 22623356] [MGI Ref ID J:188073]
Long C; Wang Y; Herrera AH; Horiuchi K; Walcheck B. 2010. In vivo role of leukocyte ADAM17 in the inflammatory and host responses during E. coli-mediated peritonitis. J Leukoc Biol 87(6):1097-101. [PubMed: 20154226] [MGI Ref ID J:162272]
Magee JA; Ikenoue T; Nakada D; Lee JY; Guan KL; Morrison SJ. 2012. Temporal changes in PTEN and mTORC2 regulation of hematopoietic stem cell self-renewal and leukemia suppression. Cell Stem Cell 11(3):415-28. [PubMed: 22958933] [MGI Ref ID J:191342]
McMahon KA; Hiew SY; Hadjur S; Veiga-Fernandes H; Menzel U; Price AJ; Kioussis D; Williams O; Brady HJ. 2007. Mll has a critical role in fetal and adult hematopoietic stem cell self-renewal. Cell Stem Cell 1(3):338-45. [PubMed: 18371367] [MGI Ref ID J:149811]
Menzel U; Kosteas T; Tolaini M; Killeen N; Roderick K; Kioussis D. 2011. Modulation of the murine CD8 gene complex following the targeted integration of human CD2-locus control region sequences. J Immunol 187(7):3712-20. [PubMed: 21880987] [MGI Ref ID J:179326]
Mortensen M; Ferguson DJ; Edelmann M; Kessler B; Morten KJ; Komatsu M; Simon AK. 2010. Loss of autophagy in erythroid cells leads to defective removal of mitochondria and severe anemia in vivo. Proc Natl Acad Sci U S A 107(2):832-7. [PubMed: 20080761] [MGI Ref ID J:156519]
Mortensen M; Soilleux EJ; Djordjevic G; Tripp R; Lutteropp M; Sadighi-Akha E; Stranks AJ; Glanville J; Knight S; Jacobsen SE; Kranc KR; Simon AK. 2011. The autophagy protein Atg7 is essential for hematopoietic stem cell maintenance. J Exp Med 208(3):455-67. [PubMed: 21339326] [MGI Ref ID J:176843]
Nern C; Wolff I; Macas J; von Randow J; Scharenberg C; Priller J; Momma S. 2009. Fusion of hematopoietic cells with Purkinje neurons does not lead to stable heterokaryon formation under noninvasive conditions. J Neurosci 29(12):3799-807. [PubMed: 19321776] [MGI Ref ID J:158171]
Odumade OA; Weinreich MA; Jameson SC; Hogquist KA. 2010. Kruppel-like factor 2 regulates trafficking and homeostasis of gammadelta T cells. J Immunol 184(11):6060-6. [PubMed: 20427763] [MGI Ref ID J:161230]
Outram SV; Hager-Theodorides AL; Shah DK; Rowbotham NJ; Drakopoulou E; Ross SE; Lanske B; Dessens JT; Crompton T. 2009. Indian hedgehog (Ihh) both promotes and restricts thymocyte differentiation. Blood 113(10):2217-28. [PubMed: 19109233] [MGI Ref ID J:145997]
Pan D; Schomber T; Kalberer CP; Terracciano LM; Hafen K; Krenger W; Hao-Shen H; Deng C; Skoda RC. 2007. Normal erythropoiesis but severe polyposis and bleeding anemia in Smad4-deficient mice. Blood 110(8):3049-55. [PubMed: 17638848] [MGI Ref ID J:149127]
Patra AK; Avots A; Zahedi RP; Schuler T; Sickmann A; Bommhardt U; Serfling E. 2012. An alternative NFAT-activation pathway mediated by IL-7 is critical for early thymocyte development. Nat Immunol 14(2):127-35. [PubMed: 23263556] [MGI Ref ID J:192612]
Rasmussen SB; Horan KA; Holm CK; Stranks AJ; Mettenleiter TC; Simon AK; Jensen SB; Rixon FJ; He B; Paludan SR. 2011. Activation of autophagy by alpha-herpesviruses in myeloid cells is mediated by cytoplasmic viral DNA through a mechanism dependent on stimulator of IFN genes. J Immunol 187(10):5268-76. [PubMed: 21998456] [MGI Ref ID J:179626]
Saba I; Kosan C; Vassen L; Moroy T. 2011. IL-7R-dependent survival and differentiation of early T-lineage progenitors is regulated by the BTB/POZ domain transcription factor Miz-1. Blood 117(12):3370-81. [PubMed: 21258009] [MGI Ref ID J:170508]
Sasaki M; Knobbe CB; Munger JC; Lind EF; Brenner D; Brustle A; Harris IS; Holmes R; Wakeham A; Haight J; You-Ten A; Li WY; Schalm S; Su SM; Virtanen C; Reifenberger G; Ohashi PS; Barber DL; Figueroa ME; Melnick A; Zuniga-Pflucker JC; Mak TW. 2012. IDH1(R132H) mutation increases murine haematopoietic progenitors and alters epigenetics. Nature 488(7413):656-9. [PubMed: 22763442] [MGI Ref ID J:186700]
Scheiermann C; Kunisaki Y; Lucas D; Chow A; Jang JE; Zhang D; Hashimoto D; Merad M; Frenette PS. 2012. Adrenergic nerves govern circadian leukocyte recruitment to tissues. Immunity 37(2):290-301. [PubMed: 22863835] [MGI Ref ID J:187383]
Schotta G; Sengupta R; Kubicek S; Malin S; Kauer M; Callen E; Celeste A; Pagani M; Opravil S; De La Rosa-Velazquez IA; Espejo A; Bedford MT; Nussenzweig A; Busslinger M; Jenuwein T. 2008. A chromatin-wide transition to H4K20 monomethylation impairs genome integrity and programmed DNA rearrangements in the mouse. Genes Dev 22(15):2048-61. [PubMed: 18676810] [MGI Ref ID J:139510]
Singbrant S; Karlsson G; Ehinger M; Olsson K; Jaako P; Miharada K; Stadtfeld M; Graf T; Karlsson S. 2010. Canonical BMP signaling is dispensable for hematopoietic stem cell function in both adult and fetal liver hematopoiesis, but essential to preserve colon architecture. Blood 115(23):4689-98. [PubMed: 20371744] [MGI Ref ID J:161541]
Srinivasan RS; Geng X; Yang Y; Wang Y; Mukatira S; Studer M; Porto MP; Lagutin O; Oliver G. 2010. The nuclear hormone receptor Coup-TFII is required for the initiation and early maintenance of Prox1 expression in lymphatic endothelial cells. Genes Dev 24(7):696-707. [PubMed: 20360386] [MGI Ref ID J:158959]
Stumpf M; Zhou X; Bluestone JA. 2013. The B7-independent isoform of CTLA-4 functions to regulate autoimmune diabetes. J Immunol 190(3):961-9. [PubMed: 23293354] [MGI Ref ID J:193019]
Tiedt R; Hao-Shen H; Sobas MA; Looser R; Dirnhofer S; Schwaller J; Skoda RC. 2008. Ratio of mutant JAK2-V617F to wild-type Jak2 determines the MPD phenotypes in transgenic mice. Blood 111(8):3931-40. [PubMed: 18160670] [MGI Ref ID J:134364]
Travis MA; Reizis B; Melton AC; Masteller E; Tang Q; Proctor JM; Wang Y; Bernstein X; Huang X; Reichardt LF; Bluestone JA; Sheppard D. 2007. Loss of integrin alpha(v)beta8 on dendritic cells causes autoimmunity and colitis in mice. Nature 449(7160):361-5. [PubMed: 17694047] [MGI Ref ID J:126778]
Tsalavos S; Segklia K; Passa O; Petryk A; O'Connor MB; Graf D. 2011. Involvement of twisted gastrulation in T cell-independent plasma cell production. J Immunol 186(12):6860-70. [PubMed: 21572028] [MGI Ref ID J:175484]
Van Ziffle JA; Lowell CA. 2009. Neutrophil-specific deletion of Syk kinase results in reduced host defense to bacterial infection. Blood 114(23):4871-82. [PubMed: 19797524] [MGI Ref ID J:155479]
Venigalla RK; McGuire VA; Clarke R; Patterson-Kane JC; Najafov A; Toth R; McCarthy PC; Simeons F; Stojanovski L; Arthur JS. 2013. PDK1 regulates VDJ recombination, cell-cycle exit and survival during B-cell development. EMBO J 32(7):1008-22. [PubMed: 23463102] [MGI Ref ID J:195305]
Vilagos B; Hoffmann M; Souabni A; Sun Q; Werner B; Medvedovic J; Bilic I; Minnich M; Axelsson E; Jaritz M; Busslinger M. 2012. Essential role of EBF1 in the generation and function of distinct mature B cell types. J Exp Med 209(4):775-92. [PubMed: 22473956] [MGI Ref ID J:183852]
Wang L; Benedito R; Bixel MG; Zeuschner D; Stehling M; Savendahl L; Haigh JJ; Snippert H; Clevers H; Breier G; Kiefer F; Adams RH. 2013. Identification of a clonally expanding haematopoietic compartment in bone marrow. EMBO J 32(2):219-30. [PubMed: 23188081] [MGI Ref ID J:193124]
Wei W; Yang Z; Tang CH; Liu L. 2011. Targeted deletion of GSNOR in hepatocytes of mice causes nitrosative inactivation of O6-alkylguanine-DNA alkyltransferase and increased sensitivity to genotoxic diethylnitrosamine. Carcinogenesis 32(7):973-7. [PubMed: 21385828] [MGI Ref ID J:173666]
Weinreich MA; Odumade OA; Jameson SC; Hogquist KA. 2010. T cells expressing the transcription factor PLZF regulate the development of memory-like CD8+ T cells. Nat Immunol 11(8):709-16. [PubMed: 20601952] [MGI Ref ID J:162317]
Welinder E; Mansson R; Mercer EM; Bryder D; Sigvardsson M; Murre C. 2011. The transcription factors E2A and HEB act in concert to induce the expression of FOXO1 in the common lymphoid progenitor. Proc Natl Acad Sci U S A 108(42):17402-7. [PubMed: 21972416] [MGI Ref ID J:177444]
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Yu S; Zhou X; Steinke FC; Liu C; Chen SC; Zagorodna O; Jing X; Yokota Y; Meyerholz DK; Mullighan CG; Knudson CM; Zhao DM; Xue HH. 2012. The TCF-1 and LEF-1 transcription factors have cooperative and opposing roles in T cell development and malignancy. Immunity 37(5):813-26. [PubMed: 23103132] [MGI Ref ID J:190675]
Zhang H; Li H; Xi HS; Li S. 2012. HIF1alpha is required for survival maintenance of chronic myeloid leukemia stem cells. Blood 119(11):2595-607. [PubMed: 22275380] [MGI Ref ID J:182536]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to C57BL/6J. The donating investigator reports that homozygous mice are not viable. Mating System Noncarrier x Hemizygote (Female x Male) 28-APR-09 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Vav1-cre)A2Kio
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Vav1-cre)A2Kio x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(Vav1-cre)A2Kio Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Vav1-cre)A2Kio
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Vav1-cre)A2Kio x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(Vav1-cre)A2Kio Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
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Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.