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| Mice harboring the Vav-iCre transgene have the mouse HS21/45 control regions directing expression of an optimized variant of Cre recombinase (iCre) to hematopoietic cells (and their progenitors, and may be useful for generating conditional mutations in hematopoietic cells. | |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 28-APR-09 Species laboratory mouse Generation N16+ (27-APR-09) Donating Investigator Dimitris Kioussis, MRC Natl Inst for Med Research Description
Mice hemizygous for this Vav-iCre transgene are viable and fertile, with the mouse HS21/45-vav control regions directing expression of an optimized variant of Cre recombinase (iCre) to hematopoietic cells (and their progenitors). Using crosses to a reporter strain, variegated germ line (testis and ovaries), and heart and gut expression is also reported. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Vav-iCre transgenic mice may be useful for generating conditional mutations in hematopoietic cells.Of note, this Vav-iCre strain (Stock No. 008610) allows reliable deletion of specific genes throughout the entire hematopoietic compartment, whereas the hCD2-iCre strain (Stock No. 008520) allows targeting to be focused to T cells and B cells.
Development
The Vav-iCre transgene was designed with the mouse vav 1 oncogene (Vav1) promoter region (containing the 2 proximal upstream DNase-I hypersensitive (HS) sites (HS21)), an optimized variant of Cre recombinase (iCre; improved with mammalian codon usage, removed putative cryptic splce sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals) and an SV40 polyA signal replacing Vav1 exon 1, and Vav1 intron 1 (containing both proximal HS sites (HS45)). The transgene was microinjected into the pronuclei of fertilized oocytes from (CBA/Ca x C57BL/10)F1 mice. Founder mice (founder line ".A2") were established and used to generate mutant mice. The resulting Vav-iCre transgenic mice were subsequently bred to wildtype C57BL/6 mice for more than 16 generations prior to arrival at The Jackson Laboratory Repository. Upon arrival, the Vav-iCre transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Vav1
010505 C57BL/6-Tg(Vav1-NUP98/HOXD13)G2Apla/J View Strains carrying other alleles of Vav1 (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Hematological Research
Immunology and Inflammation Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Vav1-cre)A2Kio | ||
|---|---|---|---|
| Allele Name | transgene insertion A2, Dimitris Kioussis | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Tg(Vav1-cre)1Kio; Vav-cre; Vav-iCre; Vav1-Cre; VavCre; | ||
| Mutation Made By | Dimitris Kioussis, MRC Natl Inst for Med Research | ||
| Strain of Origin | (CBA/Ca x C57BL/10)F1 | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Vav1, vav 1 oncogene, mouse, laboratory | ||
| Driver Note | Vav1 | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a vav promoter. This promoter was shown to be active in lymph nodes, thymus, spleen, Peyer's patches, intestinal cryptopatches, ovaries and testes. [MGI Ref ID J:81568] | ||
| Gene Symbol and Name | Tg(Vav1-cre)A2Kio, transgene insertion A2, Dimitris Kioussis | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(Vav1-cre)1Kio; Vav-cre; Vav-iCre; Vav1-Cre; VavCre; transgene insertion 1, Dimitris Kioussis; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic iCre, Melt Curve Analysis
Generic iCre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
De Boer J; Williams A; Skavdis G; Harker N; Coles M; Tolaini M; Norton T; Williams K; Roderick K; Potocnik AJ; Kioussis D. 2003. Transgenic mice with hematopoietic and lymphoid specific expression of Cre. Eur J Immunol 33(2):314-25. [PubMed: 12548562] [MGI Ref ID J:81568]
Ogilvy S; Elefanty AG; Visvader J; Bath ML; Harris AW; Adams JM. 1998. Transcriptional regulation of vav, a gene expressed throughout the hematopoietic compartment. Blood 91(2):419-30. [PubMed: 9427694] [MGI Ref ID J:45594]
Ogilvy S; Metcalf D; Gibson L; Bath ML; Harris AW; Adams JM. 1999. Promoter elements of vav drive transgene expression in vivo throughout the hematopoietic compartment. Blood 94(6):1855-63. [PubMed: 10477714] [MGI Ref ID J:140234]
Shimshek DR; Kim J; Hubner MR; Spergel DJ; Buchholz F; Casanova E; Stewart AF; Seeburg PH; Sprengel R. 2002. Codon-improved Cre recombinase (iCre) expression in the mouse. Genesis 32(1):19-26. [PubMed: 11835670] [MGI Ref ID J:78663]
Additional References
Tg(Vav1-cre)A2Kio relatedBanerjee A; Grumont R; Gugasyan R; White C; Strasser A; Gerondakis S. 2008. NF-kappaB1 and c-Rel cooperate to promote the survival of TLR4-activated B cells by neutralizing Bim via distinct mechanisms. Blood 112(13):5063-73. [PubMed: 18805964] [MGI Ref ID J:143616]
Decker T; Pasca di Magliano M; McManus S; Sun Q; Bonifer C; Tagoh H; Busslinger M. 2009. Stepwise activation of enhancer and promoter regions of the B cell commitment gene Pax5 in early lymphopoiesis. Immunity 30(4):508-20. [PubMed: 19345119] [MGI Ref ID J:147974]
Delogu A; Schebesta A; Sun Q; Aschenbrenner K; Perlot T; Busslinger M. 2006. Gene repression by Pax5 in B cells is essential for blood cell homeostasis and is reversed in plasma cells. Immunity 24(3):269-81. [PubMed: 16546096] [MGI Ref ID J:113322]
He C; Hu H; Braren R; Fong SY; Trumpp A; Carlson TR; Wang RA. 2008. c-myc in the hematopoietic lineage is crucial for its angiogenic function in the mouse embryo. Development 135(14):2467-77. [PubMed: 18550710] [MGI Ref ID J:137645]
Hou B; Reizis B; Defranco AL. 2008. Toll-like Receptors Activate Innate and Adaptive Immunity by using Dendritic Cell-Intrinsic and -Extrinsic Mechanisms. Immunity 29(2):272-82. [PubMed: 18656388] [MGI Ref ID J:139001]
Kwon K; Hutter C; Sun Q; Bilic I; Cobaleda C; Malin S; Busslinger M. 2008. Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development. Immunity 28(6):751-62. [PubMed: 18538592] [MGI Ref ID J:137719]
McMahon KA; Hiew SY; Hadjur S; Veiga-Fernandes H; Menzel U; Price AJ; Kioussis D; Williams O; Brady HJ. 2007. Mll has a critical role in fetal and adult hematopoietic stem cell self-renewal. Cell Stem Cell 1(3):338-45. [PubMed: 18371367] [MGI Ref ID J:149811]
Outram SV; Hager-Theodorides AL; Shah DK; Rowbotham NJ; Drakopoulou E; Ross SE; Lanske B; Dessens JT; Crompton T. 2009. Indian hedgehog (Ihh) both promotes and restricts thymocyte differentiation. Blood 113(10):2217-28. [PubMed: 19109233] [MGI Ref ID J:145997]
Pan D; Schomber T; Kalberer CP; Terracciano LM; Hafen K; Krenger W; Hao-Shen H; Deng C; Skoda RC. 2007. Normal erythropoiesis but severe polyposis and bleeding anemia in Smad4-deficient mice. Blood 110(8):3049-55. [PubMed: 17638848] [MGI Ref ID J:149127]
Schotta G; Sengupta R; Kubicek S; Malin S; Kauer M; Callen E; Celeste A; Pagani M; Opravil S; De La Rosa-Velazquez IA; Espejo A; Bedford MT; Nussenzweig A; Busslinger M; Jenuwein T. 2008. A chromatin-wide transition to H4K20 monomethylation impairs genome integrity and programmed DNA rearrangements in the mouse. Genes Dev 22(15):2048-61. [PubMed: 18676810] [MGI Ref ID J:139510]
Tiedt R; Hao-Shen H; Sobas MA; Looser R; Dirnhofer S; Schwaller J; Skoda RC. 2008. Ratio of mutant JAK2-V617F to wild-type Jak2 determines the MPD phenotypes in transgenic mice. Blood 111(8):3931-40. [PubMed: 18160670] [MGI Ref ID J:134364]
Travis MA; Reizis B; Melton AC; Masteller E; Tang Q; Proctor JM; Wang Y; Bernstein X; Huang X; Reichardt LF; Bluestone JA; Sheppard D. 2007. Loss of integrin alpha(v)beta8 on dendritic cells causes autoimmunity and colitis in mice. Nature 449(7160):361-5. [PubMed: 17694047] [MGI Ref ID J:126778]
Zhao C; Blum J; Chen A; Kwon HY; Jung SH; Cook JM; Lagoo A; Reya T. 2007. Loss of beta-catenin impairs the renewal of normal and CML stem cells in vivo. Cancer Cell 12(6):528-41. [PubMed: 18068630] [MGI Ref ID J:130324]
Zhao C; Chen A; Jamieson CH; Fereshteh M; Abrahamsson A; Blum J; Kwon HY; Kim J; Chute JP; Rizzieri D; Munchhof M; Vanarsdale T; Beachy PA; Reya T. 2009. Hedgehog signalling is essential for maintenance of cancer stem cells in myeloid leukaemia. Nature 458(7239):776-779. [PubMed: 19169242] [MGI Ref ID J:147298]
Zouvelou V; Passa O; Segklia K; Tsalavos S; Valenzuela DM; Economides AN; Graf D. 2009. Generation and functional characterization of mice with a conditional BMP7 allele. Int J Dev Biol 53(4):597-603. [PubMed: 19247966] [MGI Ref ID J:149134]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375/MGL377
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to C57BL/6J. The donating investigator reports that homozygous mice are not viable. Mating System Noncarrier x Hemizygote (Female x Male) 28-APR-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Vav1-cre)A2Kio
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Vav1-cre)A2Kio x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Vav1-cre)A2Kio
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Vav1-cre)A2Kio
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Vav1-cre)A2Kio x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Vav1-cre)A2Kio
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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