|Mice of this strain harbor a deletion in the killer cell lectin-like receptor, subfamily A, member 8; cytomegalovirus resistance 1 gene (Klra8, originally Ly49h).|
Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Wayne Yokoyama, Washington University Sch. Med., HHMI
Animals lacking Ly49H (Klra8, killer cell lectin-like receptor, subfamily A, member 8) expression on natural killer cells were selected from progeny of BXD8 recombinant inbred mice backcrossed to C57BL/6 for ten generations. A number of other intact killer cell lectin-like receptors and integrin alpha M (Mac1) are still effectively expressed on natural killer cells. Natural killer cell functions (e.g. upregulation of IFN-gamma after activation via NK1.1, and cellular proliferation) are unaffected by this mutation. Compared to the parental C57BL/6 strain, these mutant mice are more susceptible to murine cytomegalovirus (MCMV), but not herpes simplex virus-1 (HSV-1) or ectromelia virus infection. Homozygotes are viable and fertile.
The recombinant inbred strain BXD8 was backcrossed to C57BL/6NCr for ten generations, selecting for animals lacking Ly49H (Klra8, killer cell lectin-like receptor, subfamily A, member 8) expression on natural killer cells. Cell surface staining with anti-Ly49H-specific antibody 3D10 was used to follow the marker. Analysis of 110 polymorphic microsatellite markers by the donating laboratory indicated that the original BXD8 recombinant inbred strain carried 52% of C57BL/6-derived genome; the congenic demonstrated 100% of C57BL/6-derived genome, with the exception of Ly49H.
Strains carrying Klra8Cmv1-del allele
000084 BXD8/TyJView Strains carrying Klra8Cmv1-del (1 strain)Strains carrying other alleles of Klra8
002936 C.B6-Klra8Cmv1-r/UwaJ 025661 C.Cg-Klrk1tm1Dhr Klra8Cmv1-r/DhrJView Strains carrying other alleles of Klra8 (2 strains)
View Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
Immunology, Inflammation and Autoimmunity Research
|Allele Name||cytomegalovirus resistance 1, deletion|
|Allele Type||Not Applicable|
|Strain of Origin||BXD8/TyJ|
|Gene Symbol and Name||Klra8, killer cell lectin-like receptor, subfamily A, member 8|
|Gene Common Name(s)||Cmv-1; Cmv1; Ly49h; Ly49i6; Ly49u129; cytomegalovirus resistance 1; lymphocyte antigen 49 complex, locus H;|
|Molecular Note||Mapping experiments identified a 23 kb deletion in the Klra cluster in the BXD-8/Ty strain that contained only the Klra8 gene. This deletion correlated with an increased susceptiblity to cytomegalovirus infection. [MGI Ref ID J:79093]|
Lee SH; Girard S; Macina D; Busa M; Zafer A; Belouchi A; Gros P; Vidal SM. 2001. Susceptibility to mouse cytomegalovirus is associated with deletion of an activating natural killer cell receptor of the C-type lectin superfamily. Nat Genet 28(1):42-5. [PubMed: 11326273] [MGI Ref ID J:79093]
Bezman NA; Cedars E; Steiner DF; Blelloch R; Hesslein DG; Lanier LL. 2010. Distinct requirements of microRNAs in NK cell activation, survival, and function. J Immunol 185(7):3835-46. [PubMed: 20805417] [MGI Ref ID J:164317]
Brown MG; Dokun AO; Heusel JW; Smith HR; Beckman DL; Blattenberger EA; Dubbelde CE; Stone LR; Scalzo AA; Yokoyama WM. 2001. Vital involvement of a natural killer cell activation receptor in resistance to viral infection. Science 292(5518):934-7. [PubMed: 11340207] [MGI Ref ID J:131208]
Fodil-Cornu N; Lee SH; Belanger S; Makrigiannis AP; Biron CA; Buller RM; Vidal SM. 2008. Ly49h-deficient C57BL/6 mice: a new mouse cytomegalovirus-susceptible model remains resistant to unrelated pathogens controlled by the NK gene complex. J Immunol 181(9):6394-405. [PubMed: 18941230] [MGI Ref ID J:140724]
Geurs TL; Zhao YM; Hill EB; French AR. 2009. Ly49H engagement compensates for the absence of type I interferon signaling in stimulating NK cell proliferation during murine cytomegalovirus infection. J Immunol 183(9):5830-6. [PubMed: 19828630] [MGI Ref ID J:156608]
Lee SH; Fragoso MF; Biron CA. 2012. Cutting edge: a novel mechanism bridging innate and adaptive immunity: IL-12 induction of CD25 to form high-affinity IL-2 receptors on NK cells. J Immunol 189(6):2712-6. [PubMed: 22888135] [MGI Ref ID J:189815]
Lee SH; Kim KS; Fodil-Cornu N; Vidal SM; Biron CA. 2009. Activating receptors promote NK cell expansion for maintenance, IL-10 production, and CD8 T cell regulation during viral infection. J Exp Med 206(10):2235-51. [PubMed: 19720840] [MGI Ref ID J:153778]
Nabekura T; Kanaya M; Shibuya A; Fu G; Gascoigne NR; Lanier LL. 2014. Costimulatory molecule DNAM-1 is essential for optimal differentiation of memory natural killer cells during mouse cytomegalovirus infection. Immunity 40(2):225-34. [PubMed: 24440149] [MGI Ref ID J:209371]
Scalzo AA; Fitzgerald NA; Wallace CR; Gibbons AE; Smart YC; Burton RC; Shellam GR. 1992. The effect of the Cmv-1 resistance gene, which is linked to the natural killer cell gene complex, is mediated by natural killer cells. J Immunol 149(2):581-9. [PubMed: 1378069] [MGI Ref ID J:1426]
Thomas MF; Abdul-Wajid S; Panduro M; Babiarz JE; Rajaram M; Woodruff P; Lanier LL; Heissmeyer V; Ansel KM. 2012. Eri1 regulates microRNA homeostasis and mouse lymphocyte development and antiviral function. Blood 120(1):130-42. [PubMed: 22613798] [MGI Ref ID J:189125]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry When maintained as a live colony, homozygotes may be bred.
|Pricing for USA, Canada and Mexico shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2140.00
At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
|Pricing for International shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2782.00
Cryorecovery - Standard.
Progeny testing is not required.
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