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| A mouse prion protein promoter drives expression of the human amyloid precursor protein 695 (APP695) carboxy terminus tagged with enhanced yellow fluorescent protein (EYFP) in this transgenic line. Expression in brain, spinal cord and sciatic nerve has been confirmed by Western blot. Primary hippocampal cultures express EYFP at low levels. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 17-MAR-09 Species laboratory mouse Generation F?+ (13-MAR-09) Donating Investigator Lawrence Goldstein, UCSD-HHMI Description
A mouse prion protein promoter drives expression of the human amyloid precursor protein 695 (APP695) carboxy terminus tagged with enhanced yellow fluorescent protein (EYFP) in this transgenic line. Expression in brain, spinal cord and sciatic nerve has been confirmed by Western blot. Primary hippocampal cultures express EYFP at low levels. Mice appear normal and have no observable abnormalities.Development
The mouse prion protein promoter drives expression of the carboxy terminal portion of human amyloid precursor protein 695 (APP695) fused to enhanced yellow fluorescent protein (EYFP). The vector was introduced to CB6F1 hybrid embryos, then the line was backcrossed to C57BL/6 for an unknown number of generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
005483 129-Tg(CAG-EYFP)7AC5Nagy/J 006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J 008299 B6.Cg-Tg(NEFL-EYFP/Nefh)40Gsn/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 005620 B6;D2-Tg(S100B-EYFP)1Wjt/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 007857 C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J 007860 C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J 009422 NOD.Cg-Tg(Itgax-EYFP)1Mnz/QtngJ 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
Tg(Prnp-APP695*/EYFP)49Gsn/0
involves: BALB/c * C57BL/6
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:139717)
- mice are viable and have no observable abnormalities
This mouse can be used to support research in many areas including:
APP695 relatedNeurobiology Research
Alzheimer's Disease
strains expressing mutant APP
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
YFP relatedNeurobiology Research
Alzheimer's Disease
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Prnp-APP695*/EYFP)49Gsn | ||
|---|---|---|---|
| Allele Name | transgene insertion 49, Lawrence SB Goldstein | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | APP-YFP; | ||
| Mutation Made By | Lawrence Goldstein, UCSD-HHMI | ||
| Strain of Origin | (BALB/c x C57BL/6)F1 | ||
| Site of Expression | brain, spinal cord and sciatic nerve | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Promoter | APP695, amyloid beta (A4) precursor protein (chimeric), mouse/human chimera | ||
| Molecular Note | The mouse prion protein promoter drives expression of the carboxy terminal portion of human amyloid precursor protein 695 (APP695) fused to enhanced yellow fluorescent protein (EYFP). Expression in brain, spinal cord and sciatic nerve has been confirmed by Western blot. Primary hippocampal cultures express EYFP at low levels. [MGI Ref ID J:139717] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Tg(Prnp-APP695*/EYFP)49Gsn relatedGoldstein LS. 2008. Generation of a human APP695-EYFP fusion transgenic allele MGI Direct Data Submission :. [MGI Ref ID J:139717]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375/MGL377
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes may be bred with wildtype or C57BL/6J inbred mice. Mating System Noncarrier x Hemizygote (Female x Male) 17-MAR-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Prnp-APP695*/EYFP)49Gsn
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Prnp-APP695*/EYFP)49Gsn x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Prnp-APP695*/EYFP)49Gsn
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Prnp-APP695*/EYFP)49Gsn
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Prnp-APP695*/EYFP)49Gsn x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Prnp-APP695*/EYFP)49Gsn
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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