Strain Name:

B6;129S7-Pou4f3tm1Xia/J

Stock Number:

008645

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These mice carry a targeted mutation of the POU domain, class 4, transcription factor 3 gene. Homozygotes are deaf, have impaired balance, and display a robust circling behavior due to defects of the inner ear. This strain may be useful in studies of auditory and vestibular system development.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator David P. Corey,   Harvard Medical School/HHMI

Description
These mice carry a targeted mutation of the POU domain, class 4, transcription factor 3 gene. Homozygotes are deaf, have impaired balance, and display a robust circling behavior due to defects of the inner ear. Auditory and vestibular hair cells are lost during late embryonic and early postnatal periods. A secondary loss of spiral and vestibular ganglion neurons is also seen. This strain may be useful in studies of auditory and vestibular system development.

Development
The first exon and the majority of the second exon, encompassing most of the coding region were replaced with a PGK-neomycin cassette. The mutation was introduced using a 129S7/SvEvBrd-Hprt1+-derived AB1 embryonic stem (ES) cell line. Chimaeric mice were bred to C57BL/6J mice. The strain was maintained on a mixed C57BL/6 and 129S7/SvEvBrd-Hprt1+ background by the donating laboratory.

Related Strains

Strains carrying other alleles of Pou4f3
010560   B6.129-Pou4f3tm1.1Nat/J
003484   C57BL/6J-Pou4f3ddl/J
View Strains carrying other alleles of Pou4f3     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Deafness, Autosomal Dominant 15; DFNA15   (POU4F3)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Pou4f3tm1Xia/Pou4f3tm1Xia

        involves: 129S7/SvEvBrd * C57BL/6J
  • hearing/vestibular/ear phenotype
  • abnormal crista ampullaris neuroepithelium morphology
    • by P5, homozygotes lack hair cells in the cristae; the sensory epithelium contains only supporting cells   (MGI Ref ID J:42478)
  • abnormal organ of Corti morphology
    • the apical surface of the organ of Corti lacks stereociliary bundles and is apparently composed of a contiguous sheet of supporting cells bearing microvilli   (MGI Ref ID J:42478)
    • at P8, the spiral capillary is not located below the organ of Corti but in the spiral limbus nearby   (MGI Ref ID J:82156)
    • abnormal Deiters cell morphology
      • Deiter's-like cells are variably present   (MGI Ref ID J:42478)
      • absent Deiters cells
        • at P7/P8, no Deiters' cells are recognizable based on morphology   (MGI Ref ID J:82156)
    • abnormal cochlear hair cell development
      • at P8, only 1% of myosin VIIa-positive immature hair cells are present in the apical turn of the cochlea; not a single immature hair cell forms in the cochlear apex   (MGI Ref ID J:82156)
    • abnormal cochlear inner hair cell morphology
      • at E18.5 or P0, very small IHCs with poor luminal surface differentiation are noted in the apical region while no IHCs can be detected at the base   (MGI Ref ID J:92833)
      • absent inner hair cell stereocilia
        • at E18.5 or P0, IHCs of the apical cochlear region appear to lack stereocilia   (MGI Ref ID J:92833)
      • cochlear inner hair cell degeneration
        • by P5, homozygotes lack identifiable IHCs in the organ of Corti   (MGI Ref ID J:42478)
        • at E18.5, IHCs are readily identified in the apex but are clearly absent from the most basal 30-35% of the cochlear duct   (MGI Ref ID J:92833)
        • less severe IHC degeneration is noted at E16.5   (MGI Ref ID J:92833)
        • at P0, no IHCs can be identified in the basal parts   (MGI Ref ID J:92833)
    • abnormal cochlear outer hair cell morphology
      • at P0, both afferent and efferent fiber outgrowth to the outer hair cells is disorganized   (MGI Ref ID J:82156)
      • at E18.5, OHC morphology and patterning is markedly abnormal, esp. in the basal region of the cochlea   (MGI Ref ID J:92833)
      • abnormal outer hair cell stereociliary bundle morphology
        • at E18.5, no intact OHC stereociliary bundles can be identified with phalloidin staining   (MGI Ref ID J:92833)
        • at E18.5 or P0, the luminal surface of OHCs throughout the length of the cochlea is composed of a sheath of poorly differentiated and disorganized apical projections and poorly defined cell boundaries   (MGI Ref ID J:92833)
        • at P0, long and aberrant stereocilia-like structures are identified on the surface of some degenerating OHCs   (MGI Ref ID J:92833)
      • cochlear outer hair cell degeneration
        • by P5, homozygotes lack identifiable OHCs in the organ of Corti   (MGI Ref ID J:42478)
        • no cholinergic innervation of OHCs is detected, as shown by absence of acetylcholine esterase activity   (MGI Ref ID J:42478)
        • at E18.5, homozygotes exhibit OHC degeneration in the base and apex of the cochlea; less severe OHC degeneration is noted at E16.5   (MGI Ref ID J:92833)
    • abnormal pillar cell differentiation
      • at P7/P8, pillar cells remain undifferentiated almost throughout the cochlea   (MGI Ref ID J:82156)
    • absent cochlear hair cell stereocilia
      • the apical surface of the organ of Corti lacks stereociliary bundles   (MGI Ref ID J:42478)
      • by P8, no mature stereociliary bundles are identified in the cochlear labyrinth; however, initial generation and differentiation of cochlear hair cells is normal   (MGI Ref ID J:43752)
    • absent organ of Corti supporting cells
      • adult homozygotes show absence of supporting cells   (MGI Ref ID J:42478)
      • typically, only a single cell type, presumably the cells of Hensen, are detected above the basilar membrane   (MGI Ref ID J:42478)
    • absent pillar cells
      • pillar cells are absent or rudimentary   (MGI Ref ID J:42478)
    • cochlear hair cell degeneration
      • homozygotes exhibit progressive disorganization and loss of cochlear hair cells as early as E17.5, with nearly complete loss by P5   (MGI Ref ID J:42478)
      • at E18.5 and early postnatal stages, hair cell numbers are severely reduced in the organ of Corti via apoptosis   (MGI Ref ID J:43752)
      • at P0, most of the basal sensory epithelium has degenerated   (MGI Ref ID J:92833)
      • cochlear inner hair cell degeneration
        • by P5, homozygotes lack identifiable IHCs in the organ of Corti   (MGI Ref ID J:42478)
        • at E18.5, IHCs are readily identified in the apex but are clearly absent from the most basal 30-35% of the cochlear duct   (MGI Ref ID J:92833)
        • less severe IHC degeneration is noted at E16.5   (MGI Ref ID J:92833)
        • at P0, no IHCs can be identified in the basal parts   (MGI Ref ID J:92833)
      • cochlear outer hair cell degeneration
        • by P5, homozygotes lack identifiable OHCs in the organ of Corti   (MGI Ref ID J:42478)
        • no cholinergic innervation of OHCs is detected, as shown by absence of acetylcholine esterase activity   (MGI Ref ID J:42478)
        • at E18.5, homozygotes exhibit OHC degeneration in the base and apex of the cochlea; less severe OHC degeneration is noted at E16.5   (MGI Ref ID J:92833)
    • pillar cell degeneration
      • at E18.5, a slow degeneration of pillar cells occurs in a wave that begins from the base of the cochlea toward the apex   (MGI Ref ID J:92833)
      • at this stage, an undisrupted row of pillar cells is clearly present in the apex   (MGI Ref ID J:92833)
  • abnormal vestibular hair cell development
    • at E15.5, a small number of fresh postmitotic hair cells are abnormally retained in the supporting cell layer of vestibular sensory epithelia, indicating improper migration into the luminal cell layer   (MGI Ref ID J:43752)
    • at P8, ~20% of immature hair cells are present in mutant vestibular sensory epithelia   (MGI Ref ID J:82156)
  • absent vestibular hair cell stereocilia
    • by P8, no mature stereociliary bundles are identified in the vestibular labyrinth; however, initial generation and differentiation of vestibular hair cells is normal   (MGI Ref ID J:43752)
  • deafness
    • adult homozygotes are deaf   (MGI Ref ID J:42478)
  • increased or absent threshold for auditory brainstem response
    • unlike heterozygotes and wild-type mice which have an auditory threshold of ~50 dB SPL, homozygotes show no auditory brainstem responses at any stimulus level, including 114 dB SPL   (MGI Ref ID J:42478)
  • vestibular hair cell degeneration
    • homozygotes exhibit progressive disorganization and loss of vestibular hair cells as early as E17.5, with nearly complete loss by P5   (MGI Ref ID J:42478)
    • by P5, homozygotes lack hair cells in the otolith organs; the sensory epithelium contains only supporting cells   (MGI Ref ID J:42478)
    • at E18.5, homozygotes show only 27%, 28% and 26%, respectively, of hair cells in the sensory epithelia of the saccule, utricle and cristae   (MGI Ref ID J:43752)
    • at P4, these numbers are reduced to ~23%, 15%, and 12%, respectively, via apoptosis (as shown by TUNEL labeling)   (MGI Ref ID J:43752)
  • behavior/neurological phenotype
  • circling
    • homozygotes spend a significant time running in circles   (MGI Ref ID J:42478)
  • decreased startle reflex
    • homozygotes fail to exhibit a robust startle response upon exposure to a sharp sound   (MGI Ref ID J:42478)
  • impaired balance
    • homozygotes show an extremely poor balance, falling from a stationary drum within 10 sec; when the drum is slowly rotated, no animals remain on the drum after 5 sec   (MGI Ref ID J:42478)
  • impaired swimming
    • all homozygotes fail to remain upright and swim effectively in a tub of water   (MGI Ref ID J:42478)
  • nervous system phenotype
  • abnormal cochlear hair cell development
    • at P8, only 1% of myosin VIIa-positive immature hair cells are present in the apical turn of the cochlea; not a single immature hair cell forms in the cochlear apex   (MGI Ref ID J:82156)
  • abnormal cochlear inner hair cell morphology
    • at E18.5 or P0, very small IHCs with poor luminal surface differentiation are noted in the apical region while no IHCs can be detected at the base   (MGI Ref ID J:92833)
    • absent inner hair cell stereocilia
      • at E18.5 or P0, IHCs of the apical cochlear region appear to lack stereocilia   (MGI Ref ID J:92833)
    • cochlear inner hair cell degeneration
      • by P5, homozygotes lack identifiable IHCs in the organ of Corti   (MGI Ref ID J:42478)
      • at E18.5, IHCs are readily identified in the apex but are clearly absent from the most basal 30-35% of the cochlear duct   (MGI Ref ID J:92833)
      • less severe IHC degeneration is noted at E16.5   (MGI Ref ID J:92833)
      • at P0, no IHCs can be identified in the basal parts   (MGI Ref ID J:92833)
  • abnormal cochlear outer hair cell morphology
    • at P0, both afferent and efferent fiber outgrowth to the outer hair cells is disorganized   (MGI Ref ID J:82156)
    • at E18.5, OHC morphology and patterning is markedly abnormal, esp. in the basal region of the cochlea   (MGI Ref ID J:92833)
    • abnormal outer hair cell stereociliary bundle morphology
      • at E18.5, no intact OHC stereociliary bundles can be identified with phalloidin staining   (MGI Ref ID J:92833)
      • at E18.5 or P0, the luminal surface of OHCs throughout the length of the cochlea is composed of a sheath of poorly differentiated and disorganized apical projections and poorly defined cell boundaries   (MGI Ref ID J:92833)
      • at P0, long and aberrant stereocilia-like structures are identified on the surface of some degenerating OHCs   (MGI Ref ID J:92833)
    • cochlear outer hair cell degeneration
      • by P5, homozygotes lack identifiable OHCs in the organ of Corti   (MGI Ref ID J:42478)
      • no cholinergic innervation of OHCs is detected, as shown by absence of acetylcholine esterase activity   (MGI Ref ID J:42478)
      • at E18.5, homozygotes exhibit OHC degeneration in the base and apex of the cochlea; less severe OHC degeneration is noted at E16.5   (MGI Ref ID J:92833)
  • abnormal sensory neuron innervation pattern
    • at P0, homozygotes show only a reduced density of afferent and efferent fibers to all vestibular sensory epithelia, with no specific loss of all afferent and efferent innervation to the canal crista; no increase in radial fiber spacing is noted in the apex   (MGI Ref ID J:82156)
    • at P7/P8, innervation is severely reduced both qualitatively and quantitatively   (MGI Ref ID J:82156)
    • despite a more severe loss of hair cells (1% in cochlea vs 20% in vestibular epithelia), the cochlea retains many more sensory neurons than vestibular epithelia (46% vs 15%)   (MGI Ref ID J:82156)
    • at 6 months, homozygotes show long term retention of cochlear sensory neurons, esp. in the cochlear apex   (MGI Ref ID J:82156)
    • retention of afferents and efferents is unrelated to hair cell differentiation, as not even immature hair cells are detectable at early postnatal stages in the cochlear apex   (MGI Ref ID J:82156)
  • abnormal vestibular hair cell development
    • at E15.5, a small number of fresh postmitotic hair cells are abnormally retained in the supporting cell layer of vestibular sensory epithelia, indicating improper migration into the luminal cell layer   (MGI Ref ID J:43752)
    • at P8, ~20% of immature hair cells are present in mutant vestibular sensory epithelia   (MGI Ref ID J:82156)
  • absent cochlear hair cell stereocilia
    • the apical surface of the organ of Corti lacks stereociliary bundles   (MGI Ref ID J:42478)
    • by P8, no mature stereociliary bundles are identified in the cochlear labyrinth; however, initial generation and differentiation of cochlear hair cells is normal   (MGI Ref ID J:43752)
  • absent vestibular hair cell stereocilia
    • by P8, no mature stereociliary bundles are identified in the vestibular labyrinth; however, initial generation and differentiation of vestibular hair cells is normal   (MGI Ref ID J:43752)
  • cochlear ganglion degeneration
    • adult homozygotes have cochlear ganglia with less than one-tenth as many myelinated axons and neuronal cell bodies relative to wild-type mice   (MGI Ref ID J:42478)
    • neuronal loss is minimal at E17.5, with reduced numbers of neurons at P1 and P5, and severe depletion of neurons and myelinated fibers by several months of age   (MGI Ref ID J:42478)
    • at E18.5, mutant spiral ganglia show a ~13% loss of neurons   (MGI Ref ID J:43752)
    • at E18.5 and P0, homozygotes show a 83%-140% increase in the number of TUNEL+ apoptotic cells in the spiral ganglion   (MGI Ref ID J:43752)
    • by P4, ~29% of neurons are lost and mutant spiral ganglia become overtly smaller than wild-type   (MGI Ref ID J:43752)
    • at P7, 46% of spiral ganglion neurons are lost overall with a less severe loss in the apex   (MGI Ref ID J:82156)
  • cochlear hair cell degeneration
    • homozygotes exhibit progressive disorganization and loss of cochlear hair cells as early as E17.5, with nearly complete loss by P5   (MGI Ref ID J:42478)
    • at E18.5 and early postnatal stages, hair cell numbers are severely reduced in the organ of Corti via apoptosis   (MGI Ref ID J:43752)
    • at P0, most of the basal sensory epithelium has degenerated   (MGI Ref ID J:92833)
    • cochlear inner hair cell degeneration
      • by P5, homozygotes lack identifiable IHCs in the organ of Corti   (MGI Ref ID J:42478)
      • at E18.5, IHCs are readily identified in the apex but are clearly absent from the most basal 30-35% of the cochlear duct   (MGI Ref ID J:92833)
      • less severe IHC degeneration is noted at E16.5   (MGI Ref ID J:92833)
      • at P0, no IHCs can be identified in the basal parts   (MGI Ref ID J:92833)
    • cochlear outer hair cell degeneration
      • by P5, homozygotes lack identifiable OHCs in the organ of Corti   (MGI Ref ID J:42478)
      • no cholinergic innervation of OHCs is detected, as shown by absence of acetylcholine esterase activity   (MGI Ref ID J:42478)
      • at E18.5, homozygotes exhibit OHC degeneration in the base and apex of the cochlea; less severe OHC degeneration is noted at E16.5   (MGI Ref ID J:92833)
  • small vestibular ganglion
    • at P0, homozygotes exhibit reduced vestibular ganglia, consistent with a 77% loss of vestibular ganglion neurons at P4   (MGI Ref ID J:82156)
  • vestibular ganglion degeneration
    • adult homozygotes exhibit few or no myelinated axons beneath the otolith organs   (MGI Ref ID J:42478)
    • neuronal loss is minimal at E17.5, with reduced numbers of neurons at P1 and P5, and severe depletion of neurons and myelinated fibers by several months of age   (MGI Ref ID J:42478)
    • in contrast, no differences in the size, number, or arrangement of neurons in the retina, dorsal root and trigeminal ganglia, and midbrain are noted at E17.5, E19.5, P1, and P5   (MGI Ref ID J:42478)
    • at E16.7, mutant vestibular ganglia are significantly reduced, indicating a faster rate of vestibular ganglion neuron degeneration relative to spiral ganglion neurons   (MGI Ref ID J:43752)
    • at E18.5, 34% of vestibular ganglion neurons are already lost via apoptosis   (MGI Ref ID J:43752)
    • at E18.5 and P0, homozygotes show a 44%-68% increase in the number of TUNEL+ apoptotic cells in the vestibular ganglion   (MGI Ref ID J:43752)
    • by P4, 77% of vestibular ganglion neurons are lost via apoptosis   (MGI Ref ID J:43752)
    • at P7, 15% of vestibular ganglion neurons are lost   (MGI Ref ID J:82156)
  • vestibular hair cell degeneration
    • homozygotes exhibit progressive disorganization and loss of vestibular hair cells as early as E17.5, with nearly complete loss by P5   (MGI Ref ID J:42478)
    • by P5, homozygotes lack hair cells in the otolith organs; the sensory epithelium contains only supporting cells   (MGI Ref ID J:42478)
    • at E18.5, homozygotes show only 27%, 28% and 26%, respectively, of hair cells in the sensory epithelia of the saccule, utricle and cristae   (MGI Ref ID J:43752)
    • at P4, these numbers are reduced to ~23%, 15%, and 12%, respectively, via apoptosis (as shown by TUNEL labeling)   (MGI Ref ID J:43752)
  • growth/size/body phenotype
  • decreased body size
    • homozygotes are viable but are 10-20% smaller than wild-type littermates   (MGI Ref ID J:42478)
    • decreased body weight   (MGI Ref ID J:42478)
  • reproductive system phenotype
  • reduced fertility
    • homozygotes display reduced fertility   (MGI Ref ID J:42478)
  • homeostasis/metabolism phenotype
  • abnormal energy expenditure
    • homozygotes exhibit higher energy expenditure than wild-type littermates   (MGI Ref ID J:42478)
  • cellular phenotype
  • abnormal pillar cell differentiation
    • at P7/P8, pillar cells remain undifferentiated almost throughout the cochlea   (MGI Ref ID J:82156)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Neurodegeneration

Sensorineural Research
Hearing Defects

Pou4f3tm1Xia related

Neurobiology Research
Hearing Defects
Vestibular Defects

Sensorineural Research
Hearing Defects
Vestibular Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Pou4f3tm1Xia
Allele Name targeted mutation 1, M Xiang
Allele Type Targeted (Null/Knockout)
Common Name(s) Brn-3c-;
Mutation Made By Mengqing Xiang,   University of Med. Dent. New Jersey
Strain of Origin129S7/SvEvBrd-Hprt<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt<+>
Gene Symbol and Name Pou4f3, POU domain, class 4, transcription factor 3
Chromosome 18
Gene Common Name(s) BRN3C; Brn-3.1; Brn3.1; Brn3c; Brn3c POU domain transcription factor; DFNA15; ddl; dreidel;
Molecular Note A PGK neomycin cassette replaces the first exon and the majority of the second exon. This deletion encompasses most of the coding region for this gene. [MGI Ref ID J:42478]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Xiang M; Gan L; Li D; Chen ZY; Zhou L; O'Malley BW Jr; Klein W ; Nathans J. 1997. Essential role of POU-domain factor Brn-3c in auditory and vestibular hair cell development. Proc Natl Acad Sci U S A 94(17):9445-50. [PubMed: 9256502]  [MGI Ref ID J:42478]

Additional References

Pou4f3tm1Xia related

Atar O; Avraham KB. 2010. Anti-apoptotic factor z-Val-Ala-Asp-fluoromethylketone promotes the survival of cochlear hair cells in a mouse model for human deafness. Neuroscience 168(3):851-7. [PubMed: 20394804]  [MGI Ref ID J:161473]

Badea TC; Nathans J. 2011. Morphologies of mouse retinal ganglion cells expressing transcription factors Brn3a, Brn3b, and Brn3c: analysis of wild type and mutant cells using genetically-directed sparse labeling. Vision Res 51(2):269-79. [PubMed: 20826176]  [MGI Ref ID J:177260]

Badea TC; Williams J; Smallwood P; Shi M; Motajo O; Nathans J. 2012. Combinatorial expression of brn3 transcription factors in somatosensory neurons: genetic and morphologic analysis. J Neurosci 32(3):995-1007. [PubMed: 22262898]  [MGI Ref ID J:179888]

Hertzano R; Dror AA; Montcouquiol M; Ahmed ZM; Ellsworth B; Camper S; Friedman TB; Kelley MW; Avraham KB. 2007. Lhx3, a LIM domain transcription factor, is regulated by Pou4f3 in the auditory but not in the vestibular system. Eur J Neurosci 25(4):999-1005. [PubMed: 17331196]  [MGI Ref ID J:119779]

Hertzano R; Montcouquiol M; Rashi-Elkeles S; Elkon R; Yucel R; Frankel WN; Rechavi G; Moroy T; Friedman TB; Kelley MW; Avraham KB. 2004. Transcription profiling of inner ears from Pou4f3(ddl/ddl) identifies Gfi1 as a target of the Pou4f3 deafness gene. Hum Mol Genet 13(18):2143-53. [PubMed: 15254021]  [MGI Ref ID J:92833]

Xiang M; Gao WQ; Hasson T; Shin JJ. 1998. Requirement for Brn-3c in maturation and survival, but not in fate determination of inner ear hair cells. Development 125(20):3935-46. [PubMed: 9735355]  [MGI Ref ID J:43752]

Xiang M; Maklad A; Pirvola U; Fritzsch B. 2003. Brn3c null mutant mice show long-term, incomplete retention of some afferent inner ear innervation. BMC Neurosci 4(1):2. [PubMed: 12585968]  [MGI Ref ID J:82156]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, heterozygotes or homozygotes may be bred, but homozygotes demonstrate a circling behavior that makes them difficult to breed.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)