Strain Name:

B6;129-Krastm5Tyj/J

Stock Number:

008653

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Availability:

Cryopreserved - Ready for recovery

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This strain carries a FRT-STOP-FRT cassette in intron 1 of an oncogenic allele of the v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog gene (G12D mutation). Following introduction of the FLP recombinase, the the STOP cassette is removed, thus allowing for expression of an oncogenic K-ras transcript and protein. Hyperplasias or overt tumors form in a variety of tissues tested, such as lung, pancreas, and colon.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Tyler Jacks,   Massachusetts Institute of Technology

Description
This strain carries a FRT-STOP-FRT cassette in intron 1 of an oncogenic allele of the v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog gene (G12D mutation). Prior to expression of enhanced Flp (FLPe) or mouse codon-optimized FLP (FLPo) recombinase the allele is not expressed due to the transcriptional STOP cassette. Following introduction of the recombinase, the FRT sites are recombined and the STOP cassette is removed, thus allowing for expression of an oncogenic K-ras transcript and protein. When recombined in various tissues, the phenotype is anticipated to be the same as that seen with tissue-specific Cre-mediated recombination in the K-ras LSL strain (see Stock No. 008180). Hyperplasias or overt tumors form in a variety of tissues tested, such as lung, pancreas, and colon.

Development
A Frt-STOP-Frt (FSF) cassette was inserted into the Sal I site in intron 1 of the targeted gene, which also carries a G->D point mutation in codon 12. A puromycin resistance gene is flanked by FRT sites, 5' of the STOP element, and reads in the opposite orientation to that of the targeted locus. The mutation was created using C57BL/6-derived v26.2 embryonic stem (ES) cells. Subsequent crosses with other strains (major alleles now bred away) has placed this mutation on a mixed background composed of C57BL/6 and 129.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Krastm5Tyj allele
023590   B6(Cg)-Krastm5Tyj/J
View Strains carrying   Krastm5Tyj     (1 strain)

Strains carrying other alleles of Kras
002674   129-Krastm1Tyj/J
008185   129S/Sv-Krastm3Tyj/J
008180   129S/Sv-Krastm4Tyj/J
008179   B6.129S4-Krastm4Tyj/J
019104   B6N.Cg-Krastm4Tyj/CjDswJ
View Strains carrying other alleles of Kras     (5 strains)

Additional Web Information

FLP-FRT Systems

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Bladder Cancer   (KRAS)
Breast Cancer   (KRAS)
Cardiofaciocutaneous Syndrome 2; CFC2   (KRAS)
Gastric Cancer, Hereditary Diffuse; HDGC   (KRAS)
Lung Cancer   (KRAS)
Noonan Syndrome 3; NS3   (KRAS)
Pancreatic Cancer   (KRAS)
Schimmelpenning-Feuerstein-Mims Syndrome; SFM   (KRAS)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Krastm5Tyj/Kras+

        involves: 129S4/SvJae * C57BL/6   (conditional)
  • tumorigenesis
  • increased lung tumor incidence
    • following administration of a flp-expressing adenovirus or lentivirus   (MGI Ref ID J:172206)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation
Oncogenes

Research Tools
FLP-FRT System
      FRT-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Krastm5Tyj
Allele Name targeted mutation 5, Tyler Jacks
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) K-ras FSF; K-rasFSF-G12D;
Mutation Made ByDr. Tyler Jacks,   Massachusetts Institute of Technology
Strain of OriginC57BL/6
ES Cell Line NameV26.2
ES Cell Line StrainC57BL/6
Gene Symbol and Name Kras, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Chromosome 6
Gene Common Name(s) AI929937; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; K-ras; KI-RAS; KRAS1; KRAS2; Kirsten rat sarcoma oncogene 2, expressed; Kras-2; Kras2; NS; NS3; RASK2; c-Ki-ras; expressed sequence AI929937; p21;
Molecular Note This allele has a FRT-STOP-FRT cassette in intron 1 of the oncogenic form of the v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog gene (G12D mutation). Before expression of enhanced Flp (FLPe) or mouse codon-optimized FLP (FLPo) recombinase, the allele is not expressed due to the transcriptional STOP cassette. Following introduction of the recombinase, the FRT sites are recombined and the STOP cassette is removed resulting in expression of the mutant Kras transcript and protein. [MGI Ref ID J:140469]

Genotyping

Genotyping Information

Genotyping Protocols

Krastm4Tyj,

SEPARATED MELT


Krastm4Tyj, Separated PCR
Krastm4Tyj, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Krastm5Tyj related

Jacks T. 2008. Generation of a conditional mutant Kras allele MGI Direct Data Submission :.  [MGI Ref ID J:140469]

Lee CL; Moding EJ; Huang X; Li Y; Woodlief LZ; Rodrigues RC; Ma Y; Kirsch DG. 2012. Generation of primary tumors with Flp recombinase in FRT-flanked p53 mice. Dis Model Mech 5(3):397-402. [PubMed: 22228755]  [MGI Ref ID J:180576]

Snyder EL; Watanabe H; Magendantz M; Hoersch S; Chen TA; Wang DG; Crowley D; Whittaker CA; Meyerson M; Kimura S; Jacks T. 2013. Nkx2-1 represses a latent gastric differentiation program in lung adenocarcinoma. Mol Cell 50(2):185-99. [PubMed: 23523371]  [MGI Ref ID J:198227]

Youn JI; Kumar V; Collazo M; Nefedova Y; Condamine T; Cheng P; Villagra A; Antonia S; McCaffrey JC; Fishman M; Sarnaik A; Horna P; Sotomayor E; Gabrilovich DI. 2013. Epigenetic silencing of retinoblastoma gene regulates pathologic differentiation of myeloid cells in cancer. Nat Immunol 14(3):211-20. [PubMed: 23354483]  [MGI Ref ID J:193480]

Young NP; Crowley D; Jacks T. 2011. Uncoupling Cancer Mutations Reveals Critical Timing of p53 Loss in Sarcomagenesis. Cancer Res 71(11):4040-7. [PubMed: 21512139]  [MGI Ref ID J:172206]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, heterozygotes may be bred. Homozygotes die during embryogenesis.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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