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| This strain carries a FRT-STOP-FRT cassette in intron 1 of an oncogenic allele of the v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog gene (G12D mutation). Following introduction of the FLP recombinase, the the STOP cassette is removed, thus allowing for expression of an oncogenic K-ras transcript and protein. Hyperplasias or overt tumors form in a variety of tissues tested, such as lung, pancreas, and colon. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Dr. Tyler Jacks, Massachusetts Institute of Technology Description
This strain carries a FRT-STOP-FRT cassette in intron 1 of an oncogenic allele of the v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog gene (G12D mutation). Prior to expression of enhanced Flp (FLPe) or mouse codon-optimized FLP (FLPo) recombinase the allele is not expressed due to the transcriptional STOP cassette. Following introduction of the recombinase, the FRT sites are recombined and the STOP cassette is removed, thus allowing for expression of an oncogenic K-ras transcript and protein. When recombined in various tissues, the phenotype is anticipated to be the same as that seen with tissue-specific Cre-mediated recombination in the K-ras LSL strain (see Stock No. 008180). Hyperplasias or overt tumors form in a variety of tissues tested, such as lung, pancreas, and colon.Development
A Frt-STOP-Frt (FSF) cassette was inserted into the Sal I site in intron 1 of the targeted gene, which also carries a G->D point mutation in codon 12. A puromycin resistance gene is flanked by FRT sites, 5' of the STOP element, and reads in the opposite orientation to that of the targeted locus. The mutation was created using C57BL/6-derived v26.2 embryonic stem (ES) cells. Subsequent crosses with other strains (major alleles now bred away) has placed this mutation on a mixed background composed of C57BL/6 and 129.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Kras
002674 129-Krastm1Tyj/J 008185 129S/Sv-Krastm3Tyj/J 008180 129S/Sv-Krastm4Tyj/J 008179 B6.129S4-Krastm4Tyj/J View Strains carrying other alleles of Kras (4 strains)
Additional Web Information
FLP-FRT Systems
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Bladder Cancer (KRAS)
Breast Cancer (KRAS)
Cardiofaciocutaneous Syndrome (KRAS)
Gastric Cancer, Hereditary Diffuse; HDGC (KRAS)
Lung Cancer (KRAS)
Noonan Syndrome 3; NS3 (KRAS)
Pancreatic Cancer (KRAS)
Schimmelpenning-Feuerstein-Mims Syndrome; SFM (KRAS)
assigned by genotype
Krastm5Tyj/Kras+
involves: 129S4/SvJae * C57BL/6 (conditional)
- tumorigenesis
- increased lung tumor incidence
- following administration of a flp-expressing adenovirus or lentivirus (MGI Ref ID J:172206)
This mouse can be used to support research in many areas including:
Cancer Research
Genes Regulating Growth and Proliferation
Oncogenes
Research Tools
FLP-FRT System
FRT-flanked Sequences
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Krastm5Tyj | ||
|---|---|---|---|
| Allele Name | targeted mutation 5, Tyler Jacks | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | K-ras FSF; K-rasFSF-G12D; | ||
| Mutation Made By | Dr. Tyler Jacks, Massachusetts Institute of Technology | ||
| Strain of Origin | C57BL/6 | ||
| ES Cell Line Name | V26.2 | ||
| ES Cell Line Strain | C57BL/6 | ||
| Gene Symbol and Name | Kras, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AI929937; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; K-ras; KI-RAS; KRAS1; KRAS2; Kirsten rat sarcoma oncogene 2, expressed; Kras-2; Kras2; NS; NS3; RASK2; c-Ki-ras; expressed sequence AI929937; p21; | ||
| Molecular Note | This allele has a FRT-STOP-FRT cassette in intron 1 of the oncogenic form of the v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog gene (G12D mutation). Before expression of enhanced Flp (FLPe) or mouse codon-optimized FLP (FLPo) recombinase, the allele is not expressed due to the transcriptional STOP cassette. Following introduction of the recombinase, the FRT sites are recombined and the STOP cassette is removed resulting in expression of the mutant Kras transcript and protein. [MGI Ref ID J:140469] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Krastm4Tyj, Fast MCA
Krastm4Tyj, Separated PCR
Krastm4Tyj, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Krastm5Tyj relatedJacks T. 2008. Generation of a conditional mutant Kras allele MGI Direct Data Submission :. [MGI Ref ID J:140469]
Lee CL; Moding EJ; Huang X; Li Y; Woodlief LZ; Rodrigues RC; Ma Y; Kirsch DG. 2012. Generation of primary tumors with Flp recombinase in FRT-flanked p53 mice. Dis Model Mech 5(3):397-402. [PubMed: 22228755] [MGI Ref ID J:180576]
Youn JI; Kumar V; Collazo M; Nefedova Y; Condamine T; Cheng P; Villagra A; Antonia S; McCaffrey JC; Fishman M; Sarnaik A; Horna P; Sotomayor E; Gabrilovich DI. 2013. Epigenetic silencing of retinoblastoma gene regulates pathologic differentiation of myeloid cells in cancer. Nat Immunol 14(3):211-20. [PubMed: 23354483] [MGI Ref ID J:193480]
Young NP; Crowley D; Jacks T. 2011. Uncoupling Cancer Mutations Reveals Critical Timing of p53 Loss in Sarcomagenesis. Cancer Res 71(11):4040-7. [PubMed: 21512139] [MGI Ref ID J:172206]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintained as a live colony, heterozygotes may be bred. Homozygotes die during embryogenesis.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
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