Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donor Strain STOCK a/a Adamts20bt MitfMi-wh H2 Haplotype b

Appearance
black
Related Genotype: a/a

Description
The 120 Kb deletion in the spontaneous mutation, grizzle belly, represents the smallest complete deletion among the Kit ligand (Steel) alleles. Mice homozygous for the mutation die just before or after birth. Mice that survive to postnatal day 1 display a substantial decrease in peripheral red blood cells (RBC) and a severe anemia. Heterozygotes exhibit a mild anemia, and some decrease in peripheral RBC. In the homozygous embryo there is an absence of primordial germ cells (PGCs) by E11.5; an intermediate number of PGCs are found in the heterozygote. Like the other mutations at the Steel locus, this allele causes abnormal pigmentation. Heterozygotes can be identified by a head spot and light belly. This strain may be useful for research in hematopoiesis, pigmentation and gametogenesis.

Development
The grizzle belly (Sl-gb) mutation arose spontaneously on a mixed stock background at the MRC Radiobiology Unit in 1960. The strain was transferred to Neal Copeland and Nancy Jenkins at NCI in the 1990s where it was backcrossed to C3H/HeJ for at least 10 generations. In 1997, mice from the colony were sent to the University of Georgia and crossed to C3H/HeNCr for many generations. Subsequently, the mutation was backcrossed to C57BL/6J for 10 generations and then maintained by sibling matings. Dr. Mary Bedell at the University of Georgia donated the strain to the Repository in 2008.

Related Strains

Strains carrying other alleles of Kitl

000090	129S1/Sv-Oca2+ Tyr+ KitlSl-J/J
002993	B6.Cg-KitlSl-18H/EiJ
000124	B6.Cg-KitlSl Krt71Ca/J
000160	B6.D2-KitlSl-d/J
000291	C3FeLe.Cg-a/a Hm KitlSl Krt71Ca-J/J
001380	C3Sn.Cg-KitlSl-con/J
003252	C57BL/6J-KitlSl-20J/J
000979	STOCK KitlSl-16J/J
000161	WB.D2-KitlSl-d/J
000693	WC/ReJ KitlSl/J
100401	WCB6F1/J KitlSl KitlSl-d

View Strains carrying other alleles of Kitl     (11 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Kitl^Sl-gb/Kitl⁺

        C3.Cg-Kitl^Sl-gb

• hematopoietic system phenotype
• increased mean corpuscular hemoglobin (MGI Ref ID J:79293)
  • significantly increased compared to wild-type at P24-25
• increased mean corpuscular volume (MGI Ref ID J:79293)
  • significantly increased compared to wild-type at P24-25
• macrocytic anemia (MGI Ref ID J:79293)
  • mild at birth
• pigmentation phenotype
• abnormal ventral coat pigmentation (MGI Ref ID J:79293)
  • diluted ventrum
• head spot (MGI Ref ID J:79293)
• skin/coat/nails phenotype
• abnormal ventral coat pigmentation (MGI Ref ID J:79293)
  • diluted ventrum
• head spot (MGI Ref ID J:79293)

Kitl^Sl-gb/Kitl^Sl-gb

        C3.Cg-Kitl^Sl-gb

• lethality-prenatal/perinatal
• perinatal lethality (MGI Ref ID J:79293)
  • some are born live but all die by P2
• prenatal lethality (MGI Ref ID J:79293)
• lethality throughout fetal growth and development (MGI Ref ID J:79293)
• reproductive system phenotype
• abnormal primordial germ cell morphology (MGI Ref ID J:115437)
  • between E9.5 and 10.5, most PGCs are found with in the hindgut and these have abnormal morphology, while in wild-type embryos most PGCs are found in dorsal portions of mesentery
• decreased primordial germ cell number (MGI Ref ID J:115437)
  • at E9.5, PGCs are located primarily in the ventral axis of the hindgut while in wild-type PGCs are found primarily associated with the hindgut epithelium or in the dorsal axis of the hindgut; total PGC number in mutant embryos is 19% of wild-type
  • at E10.5, mutants have 4% of the wild-type number of PGCs
• absent primordial germ cells (MGI Ref ID J:115437)
  • at E11.5, no alkaline phosphatase-positive primordial germ cells (PGCs) are detected in genital ridges
• hematopoietic system phenotype
• low mean erythrocyte cell number (MGI Ref ID J:79293)
  • significantly lower than wild-type at P1 (RBC count is 17% of wild-type value)
• macrocytic anemia (MGI Ref ID J:79293)
  • severe at birth
• cellular phenotype
• abnormal cell migration (MGI Ref ID J:115437)
  • at E10.5, only 31% of total PGCs have migrated from hindgut, compared to 93% in wild-type
• abnormal cell proliferation (MGI Ref ID J:115437)
  • at E10.5, migratory PGCs in the hindgut are observed to proliferate
• increased apoptosis (MGI Ref ID J:115437)
  • at E10.5, many PGCs in hindgut appear to be disintegrating; abnormal PGCs in hindgut tend to be nonmotile and apoptotic

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Perinatal Lethality
      Homozygous

Hematological Research
Anemia, Iron Deficiency and Transport Defects
      macrocytic

Reproductive Biology Research
Developmental Defects Affecting Gonads
      germ cell deficient

Genes & Alleles

Gene & Allele Information

Allele Symbol		KitlSl-gb
Allele Name		grizzle belly
Allele Type		Spontaneous
Common Name(s)		Slgb;
Strain of Origin		STOCK a/a Adamts20 Mitf
Gene Symbol and Name		Kitl, kit ligand
Chromosome		10
Gene Common Name(s)		Clo; Con; DKFZp686F2250; FPH2; Gb; KL-1; MGF; Mgf; SCF; SF; SHEP7; SLF; Sl; Steel; Steel factor; cloud gray; contrasted; grizzle-belly; mast cell growth factor; steel; stem cell factor;
Molecular Note		Sequence analysis showed a proximal breakpoint ~60kb 5' of the Kitl coding region and distal breakpoint at position 5287 in the 3'UTR with ~ 120 kb deleted. [MGI Ref ID J:10748] [MGI Ref ID J:31566]

Genotyping

Genotyping Information

Genotyping Protocols

Cg-Kitl^Sl-gb, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Additional References

Kitl^Sl-gb related

Bedell MA; Cleveland LS; O'Sullivan TN; Copeland NG; Jenkins NA. 1996. Deletion and interallelic complementation analysis of Steel mutant mice. Genetics 142(3):935-44. [PubMed: 8849899]  [MGI Ref ID J:31566]

Copeland NG; Gilbert DJ; Cho BC; Donovan PJ; Jenkins NA; Cosman D; Anderson D; Lyman SD; Williams DE. 1990. Mast cell growth factor maps near the steel locus on mouse chromosome 10 and is deleted in a number of steel alleles. Cell 63(1):175-83. [PubMed: 1698554]  [MGI Ref ID J:10748]

Heaney JD; Lam MY; Michelson MV; Nadeau JH. 2008. Loss of the transmembrane but not the soluble kit ligand isoform increases testicular germ cell tumor susceptibility in mice. Cancer Res 68(13):5193-7. [PubMed: 18593919]  [MGI Ref ID J:138878]

Mahakali Zama A; Hudson FP rd; Bedell MA. 2005. Analysis of hypomorphic KitlSl mutants suggests different requirements for KITL in proliferation and migration of mouse primordial germ cells. Biol Reprod 73(4):639-47. [PubMed: 15917341]  [MGI Ref ID J:115437]

Nash DJ. 1964. Grizzle-belly allele of Sl Mouse News Lett 30:53-4.  [MGI Ref ID J:186]

Rajaraman S; Davis WS; Mahakali-Zama A; Evans HK; Russell LB; Bedell MA. 2002. An Allelic Series of Mutations in the Kit ligand Gene of Mice. II. Effects of Ethylnitrosourea-Induced Kitl Point Mutations on Survival and Peripheral Blood Cells of Kitl(Steel) Mice. Genetics 162(1):341-53. [PubMed: 12242245]  [MGI Ref ID J:79293]

Schaible RH. 1960. "Grizzle-belly" Mouse News Lett 23:31.  [MGI Ref ID J:81206]

Schaible RH. 1963. Grizzle-belly Mouse News Lett 29:48.  [MGI Ref ID J:13414]

Schaible RH. 1961. Grizzle-belly, Gb Mouse News Lett 24:38.  [MGI Ref ID J:28097]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, heterozygous mice may be bred together, to wildtype (noncarrier) siblings, or to C57BL/6J inbred mice. Homozygotes die just before or after birth.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Mouse Mutant Resource collection.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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