Strain Name:

B6.Cg-KitlSl-gb/MbeJ

Stock Number:

008656

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This spontaneous mutation in the Kit ligand, grizzle belly, is perinatally lethal in the homozygous state. Heterozygotes exhibit a mild anemia, a head spot and light belly. This strain may be useful for research in hematopoiesis, pigmentation and gametogenesis.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Spontaneous Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Donor Strain STOCK a/a Adamts20bt MitfMi-wh
H2 Haplotypeb

Appearance
black
Related Genotype: a/a

Description
The 120 Kb deletion in the spontaneous mutation, grizzle belly, represents the smallest complete deletion among the Kit ligand (Steel) alleles. Mice homozygous for the mutation die just before or after birth. Mice that survive to postnatal day 1 display a substantial decrease in peripheral red blood cells (RBC) and a severe anemia. Heterozygotes exhibit a mild anemia, and some decrease in peripheral RBC. In the homozygous embryo there is an absence of primordial germ cells (PGCs) by E11.5; an intermediate number of PGCs are found in the heterozygote. Like the other mutations at the Steel locus, this allele causes abnormal pigmentation. Heterozygotes can be identified by a head spot and light belly. This strain may be useful for research in hematopoiesis, pigmentation and gametogenesis.

Development
The grizzle belly (Sl-gb) mutation arose spontaneously on a mixed stock background at the MRC Radiobiology Unit in 1960. The strain was transferred to Neal Copeland and Nancy Jenkins at NCI in the 1990s where it was backcrossed to C3H/HeJ for at least 10 generations. In 1997, mice from the colony were sent to the University of Georgia and crossed to C3H/HeNCr for many generations. Subsequently, the mutation was backcrossed to C57BL/6J for 10 generations and then maintained by sibling matings. Dr. Mary Bedell at the University of Georgia donated the strain to the Repository in 2008.

Related Strains

View Strains carrying other alleles of Kitl     (15 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Hyperpigmentation, Familial Progressive, 2; FPH2   (KITLG)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

KitlSl-gb/Kitl+

        C3.Cg-KitlSl-gb
  • hematopoietic system phenotype
  • increased mean corpuscular hemoglobin
    • significantly increased compared to wild-type at P24-25   (MGI Ref ID J:79293)
  • increased mean corpuscular volume
    • significantly increased compared to wild-type at P24-25   (MGI Ref ID J:79293)
  • macrocytic anemia
    • mild at birth   (MGI Ref ID J:79293)
  • pigmentation phenotype
  • abnormal ventral coat pigmentation
    • diluted ventrum   (MGI Ref ID J:79293)
  • head spot   (MGI Ref ID J:79293)
  • integument phenotype
  • abnormal ventral coat pigmentation
    • diluted ventrum   (MGI Ref ID J:79293)
  • head spot   (MGI Ref ID J:79293)

KitlSl-gb/KitlSl-gb

        C3.Cg-KitlSl-gb
  • mortality/aging
  • complete perinatal lethality
    • some are born live but all die by P2   (MGI Ref ID J:79293)
  • partial lethality throughout fetal growth and development   (MGI Ref ID J:79293)
  • partial prenatal lethality   (MGI Ref ID J:79293)
  • reproductive system phenotype
  • abnormal primordial germ cell migration
    • at E10.5, only 31% of total PGCs have migrated from hindgut, compared to 93% in wild-type   (MGI Ref ID J:115437)
  • abnormal primordial germ cell morphology
    • between E9.5 and 10.5, most PGCs are found with in the hindgut and these have abnormal morphology, while in wild-type embryos most PGCs are found in dorsal portions of mesentery   (MGI Ref ID J:115437)
    • decreased primordial germ cell number
      • at E9.5, PGCs are located primarily in the ventral axis of the hindgut while in wild-type PGCs are found primarily associated with the hindgut epithelium or in the dorsal axis of the hindgut; total PGC number in mutant embryos is 19% of wild-type   (MGI Ref ID J:115437)
      • at E10.5, mutants have 4% of the wild-type number of PGCs   (MGI Ref ID J:115437)
      • absent primordial germ cells
        • at E11.5, no alkaline phosphatase-positive primordial germ cells (PGCs) are detected in genital ridges   (MGI Ref ID J:115437)
  • abnormal primordial germ cell proliferation
    • at E10.5, migratory PGCs in the hindgut are observed to proliferate   (MGI Ref ID J:115437)
  • hematopoietic system phenotype
  • low mean erythrocyte cell number
    • significantly lower than wild-type at P1 (RBC count is 17% of wild-type value)   (MGI Ref ID J:79293)
  • macrocytic anemia
    • severe at birth   (MGI Ref ID J:79293)
  • cellular phenotype
  • abnormal primordial germ cell migration
    • at E10.5, only 31% of total PGCs have migrated from hindgut, compared to 93% in wild-type   (MGI Ref ID J:115437)
  • abnormal primordial germ cell proliferation
    • at E10.5, migratory PGCs in the hindgut are observed to proliferate   (MGI Ref ID J:115437)
  • increased apoptosis
    • at E10.5, many PGCs in hindgut appear to be disintegrating; abnormal PGCs in hindgut tend to be nonmotile and apoptotic   (MGI Ref ID J:115437)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Perinatal Lethality
      Homozygous

Hematological Research
Anemia, Iron Deficiency and Transport Defects
      macrocytic

Reproductive Biology Research
Developmental Defects Affecting Gonads
      germ cell deficient

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol KitlSl-gb
Allele Name grizzle belly
Allele Type Spontaneous
Common Name(s) Slgb;
Strain of OriginSTOCK a/a Adamts20 Mitf
Gene Symbol and Name Kitl, kit ligand
Chromosome 10
Gene Common Name(s) Clo; Con; FPH2; Gb; KL-1; MGF; Mgf; SCF; SF; SHEP7; SLF; Sl; Steel; Steel factor; blaze; blz; cloud gray; contrasted; grizzle-belly; mast cell growth factor; steel; stem cell factor;
Molecular Note Sequence analysis showed a proximal breakpoint ~60kb 5' of the Kitl coding region and distal breakpoint at position 5287 in the 3'UTR with ~ 120 kb deleted. [MGI Ref ID J:10748] [MGI Ref ID J:31566]

Genotyping

Genotyping Information

Genotyping Protocols

Cg-KitlSl-gb, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

KitlSl-gb related

Bedell MA; Cleveland LS; O'Sullivan TN; Copeland NG; Jenkins NA. 1996. Deletion and interallelic complementation analysis of Steel mutant mice. Genetics 142(3):935-44. [PubMed: 8849899]  [MGI Ref ID J:31566]

Copeland NG; Gilbert DJ; Cho BC; Donovan PJ; Jenkins NA; Cosman D; Anderson D; Lyman SD; Williams DE. 1990. Mast cell growth factor maps near the steel locus on mouse chromosome 10 and is deleted in a number of steel alleles. Cell 63(1):175-83. [PubMed: 1698554]  [MGI Ref ID J:10748]

Heaney JD; Lam MY; Michelson MV; Nadeau JH. 2008. Loss of the transmembrane but not the soluble kit ligand isoform increases testicular germ cell tumor susceptibility in mice. Cancer Res 68(13):5193-7. [PubMed: 18593919]  [MGI Ref ID J:138878]

Mahakali Zama A; Hudson FP 3rd; Bedell MA. 2005. Analysis of hypomorphic KitlSl mutants suggests different requirements for KITL in proliferation and migration of mouse primordial germ cells. Biol Reprod 73(4):639-47. [PubMed: 15917341]  [MGI Ref ID J:115437]

Nash DJ. 1964. Grizzle-belly allele of Sl Mouse News Lett 30:53-4.  [MGI Ref ID J:186]

Rajaraman S; Davis WS; Mahakali-Zama A; Evans HK; Russell LB; Bedell MA. 2002. An Allelic Series of Mutations in the Kit ligand Gene of Mice. II. Effects of Ethylnitrosourea-Induced Kitl Point Mutations on Survival and Peripheral Blood Cells of Kitl(Steel) Mice. Genetics 162(1):341-53. [PubMed: 12242245]  [MGI Ref ID J:79293]

Schaible RH. 1960. "Grizzle-belly" Mouse News Lett 23:31.  [MGI Ref ID J:81206]

Schaible RH. 1963. Grizzle-belly Mouse News Lett 29:48.  [MGI Ref ID J:13414]

Schaible RH. 1961. Grizzle-belly, Gb Mouse News Lett 24:38.  [MGI Ref ID J:28097]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous mice may be bred together, to wildtype (noncarrier) siblings, or to C57BL/6J inbred mice. Homozygotes die just before or after birth.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

  • View the complete collection of spontaneous mutants in the Mouse Mutant Resource.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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