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| These Nkx2.1Cre (or BAC-Nkx2.1-Cre) transgenic mice express cre under the control of the Nkx2.1 promoter/enhancer regions and may be useful for generating conditional mutations for studying gain/loss of function or fate mapping in subgroups of brain progenitor cells (interneurons in telencephalon and hypothalamus cells), as well as developing lung, thyroid, and pituitary tissues. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names C57BL/6J-Tg(Nkx2-1-cre)2Qixu/J (Changed: 03-NOV-08 ) Type Coisogenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System +/+ sibling x Hemizygote (Female x Male) 23-JAN-09 Species laboratory mouse Generation N6+F11 (22-JAN-09)
Generation DefinitionsDonating Investigator Stewart Anderson, Weill Medical College-Cornell University Description
Mice homozygous for the Nkx2.1Cre (or BAC-Nkx2.1-Cre) transgene are viable and fertile, with cre expression directed to major subgroups of brain interneuron progenitors, developing lung, thyroid, and pituitary by the Nkx2.1 promoter/enhancer regions within the BAC transgene. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. For example, when bred with beta-galactosidase reporter mice, the first detectable Cre recombinase expression in double mutant offspring is observed on embryonic day (E)10.5 in the basal telencephalon. These Nkx2.1Cre (or BAC-Nkx2.1-Cre) transgenic mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain progenitors (including future major interneurons in telencephalon and hypothalamus cells), as well as developing lung, thyroid, and pituitary.View cre expression characterization.
Development
The 223 kb C57BL/6 mouse bacterial artificial chromosome (BAC) RP23-3H18 containing the entire Nkx2.1 (and Nkx2.9) gene, was modified by replacing the first 15 bp (including the ATG) of exon 2 of Nkx2.1 with a cre coding sequence and SV40 polyA signal. DNA from a cre modified BAC clone was used as the source for the BAC-Nkx2.1-Cre transgene, which was linearized to remove its original constructing vector backbone (pBACe3.6) and then microinjected into the pronucleus of C57BL/6 fertilized eggs. The resulting Nkx2.1Cre (or BAC-Nkx2.1-Cre) transgenic mice from founder line 2 were subsequently backcrossed to C57BL/6J for five generations prior to arrival at The Jackson Laboratory. Upon arrival, transgenic mice were bred with C57BL/6J inbred mice for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Nkx2-1
014552 STOCK Nkx2-1tm1.1(cre/ERT2)Zjh/J View Strains carrying other alleles of Nkx2-1 (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Nkx2-1-cre)2Sand | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Stewart Anderson | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | NKX2.1Cre; Nkx2-1BACCre; Nkx2.1-cre; Tg(Nkx2-1-cre)1Qixu; Tg(Nkx2-1-cre)2Qixu; Ttf-1Cre; | ||
| Mutation Made By | Stewart Anderson, Weill Medical College-Cornell University | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | Cre expression is directed to major subgroups of brain interneuron progenitors, developing lung, thyroid, and pituitary by the Nkx2.1 promoter/enhancer regions. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Nkx2-1, NK2 homeobox 1, mouse, laboratory | ||
| Driver Note | Nkx2-1 | ||
| Molecular Note | A mouse BAC clone containing the Nkx2-1 gene was engineered to express cre recombinase. Fifteen base pairs at the 5'end of exon 2 of the Nkx2-1 gene were replaced with a cre-poly(A) construct. The mutated BAC clone was then used to generate transgenic mice. This construct is expressed in brain, thyroid, pituitary, and lung, with telecephalic expression first detectable at E10.5. [MGI Ref ID J:131144] | ||
| Gene Symbol and Name | Tg(Nkx2-1-cre)2Sand, transgene insertion 2, Stewart Anderson | ||
| Chromosome | UN | ||
| Gene Common Name(s) | NKX2.1Cre; Nkx2.1-cre; Tg(Nkx2-1-cre)1Qixu; Tg(Nkx2-1-cre)2Qixu; transgene insertion 1, Qing Xu; transgene insertion 2, Qing Xu; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Nkx2-1-cre)2Sand, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Xu Q; Tam M; Anderson SA. 2008. Fate mapping Nkx2.1-lineage cells in the mouse telencephalon. J Comp Neurol 506(1):16-29. [PubMed: 17990269] [MGI Ref ID J:131144]
Additional References
Tg(Nkx2-1-cre)2Sand relatedBenadiba C; Magnani D; Niquille M; Morle L; Valloton D; Nawabi H; Ait-Lounis A; Otsmane B; Reith W; Theil T; Hornung JP; Lebrand C; Durand B. 2012. The ciliogenic transcription factor RFX3 regulates early midline distribution of guidepost neurons required for corpus callosum development. PLoS Genet 8(3):e1002606. [PubMed: 22479201] [MGI Ref ID J:183848]
Brown KN; Chen S; Han Z; Lu CH; Tan X; Zhang XJ; Ding L; Lopez-Cruz A; Saur D; Anderson SA; Huang K; Shi SH. 2011. Clonal production and organization of inhibitory interneurons in the neocortex. Science 334(6055):480-6. [PubMed: 22034427] [MGI Ref ID J:177854]
Cai Y; Zhang Q; Wang C; Zhang Y; Ma T; Zhou X; Tian M; Rubenstein JL; Yang Z. 2013. Nuclear receptor COUP-TFII-expressing neocortical interneurons are derived from the medial and lateral/caudal ganglionic eminence and define specific subsets of mature interneurons. J Comp Neurol 521(2):479-97. [PubMed: 22791192] [MGI Ref ID J:193583]
Carney RS; Mangin JM; Hayes L; Mansfield K; Sousa VH; Fishell G; Machold RP; Ahn S; Gallo V; Corbin JG. 2010. Sonic hedgehog expressing and responding cells generate neuronal diversity in the medial amygdala. Neural Dev 5:14. [PubMed: 20507551] [MGI Ref ID J:161400]
Chen L; Chatterjee M; Li JY. 2010. The mouse homeobox gene Gbx2 is required for the development of cholinergic interneurons in the striatum. J Neurosci 30(44):14824-34. [PubMed: 21048141] [MGI Ref ID J:166699]
DeMaio L; Buckley ST; Krishnaveni MS; Flodby P; Dubourd M; Banfalvi A; Xing Y; Ehrhardt C; Minoo P; Zhou B; Crandall ED; Borok Z. 2012. Ligand-independent transforming growth factor-beta type I receptor signalling mediates type I collagen-induced epithelial-mesenchymal transition. J Pathol 226(4):633-44. [PubMed: 21984393] [MGI Ref ID J:181814]
Gelman DM; Martini FJ; Nobrega-Pereira S; Pierani A; Kessaris N; Marin O. 2009. The embryonic preoptic area is a novel source of cortical GABAergic interneurons. J Neurosci 29(29):9380-9. [PubMed: 19625528] [MGI Ref ID J:151793]
Genetic Resource Sciences at The Jackson Laboratory. 2012. Expression/Specificity Patterns of Cre Alleles, 2012 MGI Direct Data Submission :. [MGI Ref ID J:184579]
Gulacsi AA; Anderson SA. 2008. Beta-catenin-mediated Wnt signaling regulates neurogenesis in the ventral telencephalon. Nat Neurosci 11(12):1383-91. [PubMed: 18997789] [MGI Ref ID J:145017]
Higginbotham H; Eom TY; Mariani LE; Bachleda A; Hirt J; Gukassyan V; Cusack CL; Lai C; Caspary T; Anton ES. 2012. Arl13b in primary cilia regulates the migration and placement of interneurons in the developing cerebral cortex. Dev Cell 23(5):925-38. [PubMed: 23153492] [MGI Ref ID J:191083]
Hoffmann AD; Peterson MA; Friedland-Little JM; Anderson SA; Moskowitz IP. 2009. sonic hedgehog is required in pulmonary endoderm for atrial septation. Development 136(10):1761-70. [PubMed: 19369393] [MGI Ref ID J:148020]
Lee S; Hjerling-Leffler J; Zagha E; Fishell G; Rudy B. 2010. The largest group of superficial neocortical GABAergic interneurons expresses ionotropic serotonin receptors. J Neurosci 30(50):16796-808. [PubMed: 21159951] [MGI Ref ID J:167596]
Li C; Li A; Li M; Xing Y; Chen H; Hu L; Tiozzo C; Anderson S; Taketo MM; Minoo P. 2009. Stabilized beta-catenin in lung epithelial cells changes cell fate and leads to tracheal and bronchial polyposis. Dev Biol 334(1):97-108. [PubMed: 19631635] [MGI Ref ID J:153553]
Li M; Krishnaveni MS; Li C; Zhou B; Xing Y; Banfalvi A; Li A; Lombardi V; Akbari O; Borok Z; Minoo P. 2011. Epithelium-specific deletion of TGF-beta receptor type II protects mice from bleomycin-induced pulmonary fibrosis. J Clin Invest 121(1):277-87. [PubMed: 21135509] [MGI Ref ID J:171845]
Londhe VA; Maisonet TM; Lopez B; Jeng JM; Xiao J; Li C; Minoo P. 2011. Conditional deletion of epithelial IKKbeta impairs alveolar formation through apoptosis and decreased VEGF expression during early mouse lung morphogenesis. Respir Res 12:134. [PubMed: 21985298] [MGI Ref ID J:181392]
Miyoshi G; Hjerling-Leffler J; Karayannis T; Sousa VH; Butt SJ; Battiste J; Johnson JE; Machold RP; Fishell G. 2010. Genetic fate mapping reveals that the caudal ganglionic eminence produces a large and diverse population of superficial cortical interneurons. J Neurosci 30(5):1582-94. [PubMed: 20130169] [MGI Ref ID J:157935]
Riccio O; Murthy S; Szabo G; Vutskits L; Kiss JZ; Vitalis T; Lebrand C; Dayer AG. 2012. New pool of cortical interneuron precursors in the early postnatal dorsal white matter. Cereb Cortex 22(1):86-98. [PubMed: 21616983] [MGI Ref ID J:191180]
Ring LE; Zeltser LM. 2010. Disruption of hypothalamic leptin signaling in mice leads to early-onset obesity, but physiological adaptations in mature animals stabilize adiposity levels. J Clin Invest 120(8):2931-41. [PubMed: 20592471] [MGI Ref ID J:163776]
Song H; Yao E; Lin C; Gacayan R; Chen MH; Chuang PT. 2012. Functional characterization of pulmonary neuroendocrine cells in lung development, injury, and tumorigenesis. Proc Natl Acad Sci U S A 109(43):17531-6. [PubMed: 23047698] [MGI Ref ID J:190366]
Sunmonu NA; Chen L; Li JY. 2009. Misexpression of Gbx2 throughout the mesencephalon by a conditional gain-of-function transgene leads to deletion of the midbrain and cerebellum in mice. Genesis 47(10):667-673. [PubMed: 19603509] [MGI Ref ID J:153564]
Tiozzo C; De Langhe S; Yu M; Londhe VA; Carraro G; Li M; Li C; Xing Y; Anderson S; Borok Z; Bellusci S; Minoo P. 2009. Deletion of Pten expands lung epithelial progenitor pools and confers resistance to airway injury. Am J Respir Crit Care Med 180(8):701-12. [PubMed: 19574443] [MGI Ref ID J:167962]
Wei B; Huang Z; He S; Sun C; You Y; Liu F; Yang Z. 2012. The onion skin-like organization of the septum arises from multiple embryonic origins to form multiple adult neuronal fates. Neuroscience 222:110-23. [PubMed: 22820053] [MGI Ref ID J:192502]
Xing Y; Li C; Hu L; Tiozzo C; Li M; Chai Y; Bellusci S; Anderson S; Minoo P. 2008. Mechanisms of TGFbeta inhibition of LUNG endodermal morphogenesis: the role of TbetaRII, Smads, Nkx2.1 and Pten. Dev Biol 320(2):340-50. [PubMed: 18602626] [MGI Ref ID J:138200]
Xing Y; Li C; Li A; Sridurongrit S; Tiozzo C; Bellusci S; Borok Z; Kaartinen V; Minoo P. 2010. Signaling via Alk5 controls the ontogeny of lung Clara cells. Development 137(5):825-33. [PubMed: 20147383] [MGI Ref ID J:157922]
Yee CL; Wang Y; Anderson S; Ekker M; Rubenstein JL. 2009. Arcuate nucleus expression of NKX2.1 and DLX and lineages expressing these transcription factors in neuropeptide Y(+), proopiomelanocortin(+), and tyrosine hydroxylase(+) neurons in neonatal and adult mice. J Comp Neurol 517(1):37-50. [PubMed: 19711380] [MGI Ref ID J:161578]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic carrier mice may be bred together, to wildtype (noncarrier) siblings, or to C57BL/6J inbred mice. Mating System +/+ sibling x Hemizygote (Female x Male) 23-JAN-09 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Nkx2-1-cre)2Sand
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Nkx2-1-cre)2Sand x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(Nkx2-1-cre)2Sand Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Nkx2-1-cre)2Sand
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Nkx2-1-cre)2Sand x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(Nkx2-1-cre)2Sand Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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