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| EGFP-Fmn1-IV mice express Enhanced Green Fluorescent Protein (EGFP) from the Fmn1 (formin 1) locus. This mutant mouse strain may be useful in studies of cytoskeleton regulation, cell motility, and vesicle endocytosis. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Philip Leder, Harvard Medical School Description
These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted in-frame into the Fmn1 locus. Expression of the EGFP is detected in the cytoplasm and localized along microtubules. This strain serves as a reporter strain, with EGFP expression mimicking endogenous expression patterns. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of cytoskeleton regulation, cell motility, and vesicle endocytosis.Development
A targeting vector containing a loxP site flanked PGKneo cassette and sequence encoding EGFP (Green Fluorescent Protein) was inserted upstream of exon 6. The endogenous Fmn1 promoter drives expression of the EGFP through the in-frame insertion of the EGFP coding sequence to exon 6 of the Fmn1 gene, generating a fusion gene product (protein). The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to Cre recombinase expressing mice to remove the PGKneo cassette. The mice were crossed to C57BL/6J, and then backcrossed to 129 for 10 generations before arriving at The Jackson Laboratory. Upon arrival, mice were bred to 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
002225 B10.M-H2f/nMob Fmn1ld-2J/J 008665 FVB.129S6-Fmn1tm2Made/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Fmn1tm1Made/Fmn1+
129S6/SvEvTac
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:137209)
- mice were only used for expression analysis
This mouse can be used to support research in many areas including:
Cell Biology Research
Vesicular Trafficking
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Fmn1tm1Made | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Markus Dettenhofer | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | EGFP-Fmn1-IV; | ||
| Mutation Made By | Philip Leder, Harvard Medical School | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | TC-1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Gene Symbol and Name | Fmn1, formin 1 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | BB164513; DKFZp686C2281; DKFZp686G2387; FLJ45135; FMN; Fmn; LD; MGC125288; MGC125289; expressed sequence BB164513; formin; formin-1; ld; limb deformity; | ||
| Molecular Note | An EGFP gene was inserted inframe directly upstream of exon 6 to create a fusion product. A floxed neo cassette was also inserted upstream of exon 6 but was removed by germ line, cre-mediated recombination. [MGI Ref ID J:137209] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Dettenhofer M; Zhou F; Leder P. 2008. Formin 1-isoform IV deficient cells exhibit defects in cell spreading and focal adhesion formation. PLoS ONE 3(6):e2497. [PubMed: 18560567] [MGI Ref ID J:137209]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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