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| EGFP-Fmn1-IV mice express Enhanced Green Fluorescent Protein (EGFP) from the Fmn1 (formin 1) locus. This mutant mouse strain may be useful in studies of cytoskeleton regulation, cell motility, and vesicle endocytosis. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names 129(Cg)-Fmn1tm1Made/J (Changed: 02-FEB-10 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Philip Leder, Harvard Medical School Description
These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted in-frame into the Fmn1 locus. Expression of the EGFP is detected in the cytoplasm and localized along microtubules. This strain serves as a reporter strain, with EGFP expression mimicking endogenous expression patterns. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of cytoskeleton regulation, cell motility, and vesicle endocytosis.Development
A targeting vector containing a loxP site flanked PGKneo cassette and sequence encoding EGFP (Green Fluorescent Protein) was inserted upstream of exon 6. The endogenous Fmn1 promoter drives expression of the EGFP through the in-frame insertion of the EGFP coding sequence to exon 6 of the Fmn1 gene, generating a fusion gene product (protein). The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to Cre recombinase expressing mice to remove the PGKneo cassette. The mice were crossed to C57BL/6J, and then backcrossed to 129 for 10 generations before arriving at The Jackson Laboratory. Upon arrival, mice were bred to 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed 3 of 27 markers (on chromsomes 3, 9 and 18) that were not 129S allele-type. These data suggest C57BL/6 or 129X1 (and perhaps other) genetic contamination prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (240 strains)
002225 B10.M-H2f/nMob Fmn1ld-2J/J 008665 FVB.129S6-Fmn1tm2Made/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Fmn1tm1Made/Fmn1+
129S6/SvEvTac
- no phenotypic analysis
- *normal* no phenotypic analysis
- mice were only used for expression analysis (MGI Ref ID J:137209)
This mouse can be used to support research in many areas including:
Cell Biology Research
Vesicular Trafficking
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Fmn1tm1Made | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Markus Dettenhofer | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | EGFP-Fmn1-IV; | ||
| Mutation Made By | Philip Leder, Harvard Medical School | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | TC-1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Site of Expression | EGFP is detected in the cytoplasm and localized along microtubules. | ||
| Gene Symbol and Name | Fmn1, formin 1 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | BB164513; DKFZp686C2281; DKFZp686G2387; FLJ45135; FMN; Fmn; LD; MGC125288; MGC125289; expressed sequence BB164513; formin; formin-1; ld; limb deformity; | ||
| Molecular Note | An EGFP gene was inserted inframe directly upstream of exon 6 to create a fusion product. A floxed neo cassette was also inserted upstream of exon 6 but was removed by germ line, cre-mediated recombination. [MGI Ref ID J:137209] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fmn1tm1Made, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Dettenhofer M; Zhou F; Leder P. 2008. Formin 1-isoform IV deficient cells exhibit defects in cell spreading and focal adhesion formation. PLoS ONE 3(6):e2497. [PubMed: 18560567] [MGI Ref ID J:137209]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
General inquiriesContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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