Strain Name:

B6.129S7-Atoh1tm3Hzo/J

Stock Number:

008681

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These mice possess loxP sites on either side of the coding region of the atonal homolog 1 gene (Atoh1). When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene and elucidating its role in cell fate commitment.

Description

Strain Information

Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   09-OCT-10
Specieslaboratory mouse
GenerationN11+F13 (04-AUG-14)
Generation Definitions
 
Donating Investigator Huda Zoghbi,   Baylor College of Medicine

Description
These mice possess loxP sites on either side of the coding region of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in further elucidating the role of this gene in cell fate commitment.

Development
A loxP site was inserted 300 bp upstream of the translocation start site, and a second was placed immediately 3' of the coding sequence, followed by an FRT-flanked PGK-neomycin selection cassette. The mutation was introduced to AB2.2 129S7/SvEvBrd-Hprt1+-derived embryonic stem (ES) cell line. Mice were crossed with an 129SvEv background hACT-Flpe strain to excise the selection cassette. This strain was backcrossed to C57BL/6 for more than ten generations by the donating laboratory.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Atoh1
013593   B6.129S-Atoh1tm4.1Hzo/J
013594   B6.129S-Atoh1tm5.1(Cre/PGR)Hzo/J
013595   B6.129S-Atoh1tm6.1Hzo/J
005970   B6.129S7-Atoh1tm2Hzo/J
011104   B6.Cg-Tg(Atoh1-cre)1Bfri/J
007684   STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J
View Strains carrying other alleles of Atoh1     (6 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Atoh1tm3Hzo/Atoh1tm3Hzo

        involves: 129S7/SvEvBrd   (conditional)
  • nervous system phenotype
  • abnormal neuronal precursor proliferation
    • after adenoviral cre infection, proliferation of granule neuron precursors in response to stimulation by sonic hedgehog for 3 days is decreased   (MGI Ref ID J:155047)
  • cellular phenotype
  • abnormal neuronal precursor proliferation
    • after adenoviral cre infection, proliferation of granule neuron precursors in response to stimulation by sonic hedgehog for 3 days is decreased   (MGI Ref ID J:155047)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Genes Regulating Growth and Proliferation

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Atoh1tm3Hzo
Allele Name targeted mutation 3, Huda Y Zoghbi
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Atoh1flox; Math1flox;
Mutation Made By Huda Zoghbi,   Baylor College of Medicine
Strain of Origin129S7/SvEvBrd-Hprt
ES Cell Line NameAB2.2
ES Cell Line Strain129S7/SvEvBrd-Hprt
Gene Symbol and Name Atoh1, atonal homolog 1 (Drosophila)
Chromosome 6
Gene Common Name(s) ATH1; HATH1; MATH-1; Math1; RGD1565171; bHLHa14;
Molecular Note A loxP site was inserted 300 bp upstream of the translation start site and a second loxP site was inserted along with an Frt flanked neo cassette immediately 3' of the coding region. The neo cassette was removed by in vivo Flp-mediated recombination leaving the entire coding region flanked with loxP sites. [MGI Ref ID J:128312]

Genotyping

Genotyping Information

Genotyping Protocols

Atoh1tm3Hzo, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Shroyer NF; Helmrath MA; Wang VY; Antalffy B; Henning SJ; Zoghbi HY. 2007. Intestine-specific ablation of mouse atonal homolog 1 (Math1) reveals a role in cellular homeostasis. Gastroenterology 132(7):2478-88. [PubMed: 17570220]  [MGI Ref ID J:128312]

Additional References

Atoh1tm3Hzo related

Bjerknes M; Khandanpour C; Moroy T; Fujiyama T; Hoshino M; Klisch TJ; Ding Q; Gan L; Wang J; Martin MG; Cheng H. 2012. Origin of the brush cell lineage in the mouse intestinal epithelium. Dev Biol 362(2):194-218. [PubMed: 22185794]  [MGI Ref ID J:180770]

Bossuyt W; Kazanjian A; De Geest N; Van Kelst S; De Hertogh G; Geboes K; Boivin GP; Luciani J; Fuks F; Chuah M; VandenDriessche T; Marynen P; Cools J; Shroyer NF; Hassan BA. 2009. Atonal homolog 1 is a tumor suppressor gene. PLoS Biol 7(2):e39. [PubMed: 19243219]  [MGI Ref ID J:145954]

Cai T; Seymour ML; Zhang H; Pereira FA; Groves AK. 2013. Conditional deletion of Atoh1 reveals distinct critical periods for survival and function of hair cells in the organ of Corti. J Neurosci 33(24):10110-22. [PubMed: 23761906]  [MGI Ref ID J:199162]

Chonko KT; Jahan I; Stone J; Wright MC; Fujiyama T; Hoshino M; Fritzsch B; Maricich SM. 2013. Atoh1 directs hair cell differentiation and survival in the late embryonic mouse inner ear. Dev Biol 381(2):401-10. [PubMed: 23796904]  [MGI Ref ID J:200775]

Durand A; Donahue B; Peignon G; Letourneur F; Cagnard N; Slomianny C; Perret C; Shroyer NF; Romagnolo B. 2012. Functional intestinal stem cells after Paneth cell ablation induced by the loss of transcription factor Math1 (Atoh1). Proc Natl Acad Sci U S A 109(23):8965-70. [PubMed: 22586121]  [MGI Ref ID J:184755]

Flora A; Klisch TJ; Schuster G; Zoghbi HY. 2009. Deletion of Atoh1 disrupts Sonic Hedgehog signaling in the developing cerebellum and prevents medulloblastoma. Science 326(5958):1424-7. [PubMed: 19965762]  [MGI Ref ID J:155047]

Gerbe F; van Es JH; Makrini L; Brulin B; Mellitzer G; Robine S; Romagnolo B; Shroyer NF; Bourgaux JF; Pignodel C; Clevers H; Jay P. 2011. Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium. J Cell Biol 192(5):767-80. [PubMed: 21383077]  [MGI Ref ID J:170320]

Ho LL; Sinha A; Verzi M; Bernt KM; Armstrong SA; Shivdasani RA. 2013. DOT1L-mediated H3K79 methylation in chromatin is dispensable for Wnt pathway-specific and other intestinal epithelial functions. Mol Cell Biol 33(9):1735-45. [PubMed: 23428873]  [MGI Ref ID J:203756]

Kiesslich R; Goetz M; Angus EM; Hu Q; Guan Y; Potten C; Allen T; Neurath MF; Shroyer NF; Montrose MH; Watson AJ. 2007. Identification of epithelial gaps in human small and large intestine by confocal endomicroscopy. Gastroenterology 133(6):1769-78. [PubMed: 18054549]  [MGI Ref ID J:135499]

Kim TH; Escudero S; Shivdasani RA. 2012. Intact function of Lgr5 receptor-expressing intestinal stem cells in the absence of Paneth cells. Proc Natl Acad Sci U S A 109(10):3932-7. [PubMed: 22355124]  [MGI Ref ID J:182139]

Kim TH; Li F; Ferreiro-Neira I; Ho LL; Luyten A; Nalapareddy K; Long H; Verzi M; Shivdasani RA. 2014. Broadly permissive intestinal chromatin underlies lateral inhibition and cell plasticity. Nature 506(7489):511-5. [PubMed: 24413398]  [MGI Ref ID J:207494]

Kim TH; Shivdasani RA. 2011. Genetic Evidence That Intestinal Notch Functions Vary Regionally and Operate through a Common Mechanism of Math1 Repression. J Biol Chem 286(13):11427-33. [PubMed: 21282114]  [MGI Ref ID J:170937]

Klisch TJ; Xi Y; Flora A; Wang L; Li W; Zoghbi HY. 2011. In vivo Atoh1 targetome reveals how a proneural transcription factor regulates cerebellar development. Proc Natl Acad Sci U S A 108(8):3288-93. [PubMed: 21300888]  [MGI Ref ID J:169298]

Li X; Newbern JM; Wu Y; Morgan-Smith M; Zhong J; Charron J; Snider WD. 2012. MEK Is a Key Regulator of Gliogenesis in the Developing Brain. Neuron 75(6):1035-50. [PubMed: 22998872]  [MGI Ref ID J:188328]

Maksimovic S; Nakatani M; Baba Y; Nelson AM; Marshall KL; Wellnitz SA; Firozi P; Woo SH; Ranade S; Patapoutian A; Lumpkin EA. 2014. Epidermal Merkel cells are mechanosensory cells that tune mammalian touch receptors. Nature 509(7502):617-21. [PubMed: 24717432]  [MGI Ref ID J:213252]

Maricich SM; Morrison KM; Mathes EL; Brewer BM. 2012. Rodents rely on Merkel cells for texture discrimination tasks. J Neurosci 32(10):3296-300. [PubMed: 22399751]  [MGI Ref ID J:182735]

Maricich SM; Wellnitz SA; Nelson AM; Lesniak DR; Gerling GJ; Lumpkin EA; Zoghbi HY. 2009. Merkel cells are essential for light-touch responses. Science 324(5934):1580-2. [PubMed: 19541997]  [MGI Ref ID J:149981]

Maricich SM; Xia A; Mathes EL; Wang VY; Oghalai JS; Fritzsch B; Zoghbi HY. 2009. Atoh1-lineal neurons are required for hearing and for the survival of neurons in the spiral ganglion and brainstem accessory auditory nuclei. J Neurosci 29(36):11123-33. [PubMed: 19741118]  [MGI Ref ID J:152678]

McDole JR; Wheeler LW; McDonald KG; Wang B; Konjufca V; Knoop KA; Newberry RD; Miller MJ. 2012. Goblet cells deliver luminal antigen to CD103+ dendritic cells in the small intestine. Nature 483(7389):345-9. [PubMed: 22422267]  [MGI Ref ID J:182576]

Morrison KM; Miesegaes GR; Lumpkin EA; Maricich SM. 2009. Mammalian Merkel cells are descended from the epidermal lineage. Dev Biol 336(1):76-83. [PubMed: 19782676]  [MGI Ref ID J:154915]

Noah TK; Kazanjian A; Whitsett J; Shroyer NF. 2010. SAM pointed domain ETS factor (SPDEF) regulates terminal differentiation and maturation of intestinal goblet cells. Exp Cell Res 316(3):452-65. [PubMed: 19786015]  [MGI Ref ID J:156638]

Pan N; Jahan I; Kersigo J; Kopecky B; Santi P; Johnson S; Schmitz H; Fritzsch B. 2010. Conditional deletion of Atoh1 using Pax2-Cre results in viable mice without differentiated cochlear hair cells that have lost most of the organ of Corti. Hear Res :. [PubMed: 21146598]  [MGI Ref ID J:169044]

Van Keymeulen A; Mascre G; Youseff KK; Harel I; Michaux C; De Geest N; Szpalski C; Achouri Y; Bloch W; Hassan BA; Blanpain C. 2009. Epidermal progenitors give rise to Merkel cells during embryonic development and adult homeostasis. J Cell Biol 187(1):91-100. [PubMed: 19786578]  [MGI Ref ID J:163216]

van Es JH; de Geest N; van de Born M; Clevers H; Hassan BA. 2010. Intestinal stem cells lacking the Math1 tumour suppressor are refractory to Notch inhibitors. Nat Commun 1:18. [PubMed: 20975679]  [MGI Ref ID J:205667]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, homozygotes may be bred.
Mating SystemHomozygote x Homozygote         (Female x Male)   09-OCT-10
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHomozygous for Atoh1tm3Hzo  
Price per Pair (US dollars $)Pair Genotype
$478.00Homozygous for Atoh1tm3Hzo x Homozygous for Atoh1tm3Hzo  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHomozygous for Atoh1tm3Hzo  
Price per Pair (US dollars $)Pair Genotype
$621.40Homozygous for Atoh1tm3Hzo x Homozygous for Atoh1tm3Hzo  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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