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| These TRE-VEGFR2T transgenic mice express a truncated mouse Kdr (kinase insert domain protein receptor) gene under the control of a tetracycline-responsive promoter element (TRE or tetO). When bred with a mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), a bitransgenic animal can be produced that has tissue-specific expression of Kdr that can be regulated with the tetracycline analog, doxycycline. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Rong Wang, Univ of California Medical Center (UCSF) Description
These transgenic mice express a truncated mouse Kdr (kinase insert domain protein receptor) gene under the control of a tetracycline-responsive promoter element (TRE or tetO). The truncated gene encodes amino acids 1 through 828, which includes the cytoplasmic tyrosine kinase domain, and has a hemaglutin (HA) tag fused at the C terminus. When bred with a transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), a bitransgenic animal can be produced that has tissue-specific expression of Kdr that can be regulated with the tetracycline analog, doxycycline.For example, when bred to a strain expressing tTA in liver (see Stock No. 003563) and a targeted mutation of Kdr (see Stock No. 002938) , this mutant mouse strain may be useful in studies of liver organogenesis.
Development
The TRE-VEGFR2T transgene was designed with a truncated mouse Kdr (kinase insert domain protein receptor) gene, under the control of heptamerized tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) sequences and a cytomegalovirus minimal promoter with a beta-globin poly A signal. This transgene was injected into fertilized FVB/N mouse eggs. Founder line 4377.5 was subsequently established.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Kdr
002938 B6.129-Kdrtm1Jrt/J View Strains carrying other alleles of Kdr (1 strain)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Kdrtm1Jrt/Kdr+ Tg(tetO-Kdr*)4377.5Rwng/0 Tg(tTALap)5Bjd/0
involves: 129S1/Sv * 129X1/SvJ * FVB/N * NMRI
- liver/biliary system phenotype
- abnormal liver development (MGI Ref ID J:100427)
- at E12.5, liver vasculature is less organized and vessels are dilated
- at E13.5, microvascular network is more disorganized with fewer branches than in control livers; newborns have very little microvascular network is observed
Tg(tetO-Kdr*)4377.5Rwng/0 Tg(tTALap)5Bjd/0
involves: FVB/N * NMRI
- lethality-prenatal/perinatal
- perinatal lethality (MGI Ref ID J:100427)
- between 25% and 38% of pups are stillborn
- liver/biliary system phenotype
- *normal* liver/biliary system phenotype (MGI Ref ID J:100427)
- liver glycogen and albumin levels are comparable to control animals
- abnormal liver morphology (MGI Ref ID J:100427)
- livers from neonates display a decrease in blood vessels
- livers of all newborn animals are dark red in appearance in contrast to pink color of control livers
- fewer endothelial cells (ECs) are present in mutant liver vasculature; ECs are present but are less organized and more discontinuous than in controls
- fewer or collapsed-appearing sinusoidal channels are observed
- livers have a sparse and poorly developed sinusoidal network with numerous blood cells in the disrupted sinusoidal spaces
- parenchymal cells contain very few or no lipid droplets
- morphology of space of Disse is abnormal, showing large gaps between sinusoidal epithelial cells (SECs) and hepatocytes
- sinusoidal epithelium lacks fenestrations seen in normal livers
- abnormal hepatocyte morphology (MGI Ref ID J:100427)
- hepatocytes are more scattered than in control livers and many are oddly shaped with fewer microvilli projections into the space of Disse
- abnormal liver physiology (MGI Ref ID J:100427)
- there is increase in red blood cells in liver due to defective circulation
- livers show a significant defect in lipoprotein uptake compared to controls
- jaundice (MGI Ref ID J:100427)
- 30 to 63% of newborns are jaundiced; suppression of transgenic Kdr by doxycycline treatement of dams during embryogenesis eliminates jaundice that is observed in untreated animals
- cardiovascular system phenotype
- abnormal blood vessel morphology (MGI Ref ID J:100427)
- decreased number of blood vessels in liver
- homeostasis/metabolism phenotype
- *normal* homeostasis/metabolism phenotype (MGI Ref ID J:100427)
- serum bilirubin levels are normal
This mouse can be used to support research in many areas including:
Research Tools
Cardiovascular Research
Tetop Tet System
Cell Biology Research
Neurobiology Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Responsive Strains
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(tetO-Kdr*)4377.5Rwng | ||
|---|---|---|---|
| Allele Name | transgene insertion 4377.5, Rong Wang | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | TRE-VEGFR2T; | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | Kdr, kinase insert domain protein receptor, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | The transgene construct was designed with a truncated mouse Kdr (kinase insert domain protein receptor) gene, under the control of heptamerized tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) sequences and a cytomegalovirus minimal promoter with a beta-globin poly A signal. The truncated gene encodes amino acids 1 through 828, which includes the cytoplasmic tyrosine kinase domain, and has a hemaglutin (HA) tag fused at the C terminus.This transgene was injected into fertilized FVB/N mouse eggs. Founder line 4377.5 was subsequently established. [MGI Ref ID J:100427] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tetO-Kdr*), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Carpenter B; Lin Y; Stoll S; Raffai RL; McCuskey R; Wang R. 2005. VEGF is crucial for the hepatic vascular development required for lipoprotein uptake. Development 132(14):3293-303. [PubMed: 15944181] [MGI Ref ID J:100427]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice may be bred as hemizygotes. The Donating Investigator has not attempted to make the strain homozygous.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Terms of Use
General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
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