Strain Name:

FVB-Tg(tetO-MET)23Rwng/J

Stock Number:

008695

Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These TRE-MET transgenic mice express human MET (met proto-oncogene (hepatocyte growth factor receptor)) gene under the control of a tetracycline-responsive promoter element (TRE or tetO). When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein or tetracycline-controlled transactivator protein, a bitransgenic animal can be produced that has tissue-specific expression of MET that can be regulated with the tetracycline analog, doxycycline.

Description

Strain Information

Type Coisogenic; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   24-FEB-09
Specieslaboratory mouse
GenerationF?+ (23-FEB-09)
 
Donating Investigator Rong Wang,   Univ of California Medical Center (UCSF)

Description
These transgenic mice express the human MET (met proto-oncogene (hepatocyte growth factor receptor)) gene under the control of a tetracycline-responsive promoter element (TRE or tetO). When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), a bitransgenic animal can be produced that has tissue-specific expression of MET that can be regulated with the tetracycline analog, doxycycline. When bred to mice carrying the transgene LAP-tTA, (see Stock No. 003563 for example), the resulting bi-transgenic mice have inducible overexpression of MET in hepatocytes and represent an effective tool for studying hepatocellular carcinoma.

Development
The TRE-MET transgene was designed with the human MET (met proto-oncogene (hepatocyte growth factor receptor)) coding sequence, under the control of heptamerized tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) sequences and a cytomegalovirus (hCMV) minimal promoter with a beta-globin poly A signal. This transgene was injected into fertilized FVB/N mouse oocytes. Founder line 23 was subsequently established.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of MET
002675   FVB/N-Tg(MtTPRMET)243Lng/J
002775   FVB/N-Tg(MtTPRMET)773Lng/J
View Strains carrying other alleles of MET     (2 strains)

Strains carrying other alleles of tetO
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
003762   B6.Cg-Tg(tetFosb)4468Nes/J
007051   B6.Cg-Tg(tetO-APPSwInd)102Dbo/J
007052   B6.Cg-Tg(tetO-APPSwInd)107Dbo/J
007049   B6.Cg-Tg(tetO-APPSwInd)885Dbo/J
007618   B6.Cg-Tg(tetO-Arntl)1Jt/J
008277   B6.Cg-Tg(tetO-Clockm1Jt)CL57Jt/J
008468   B6.Cg-Tg(tetO-DTA)1Gfi/J
006234   B6.Cg-Tg(tetO-cre)1Jaw/J
005738   B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
002709   B6;C3-Tg(TettTALuc)1Dgs/J
008344   B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J
008082   B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J
010577   B6;SJL-Tg(tetO-Erbb2*)8-4Jek/J
002621   B6;SJL-Tg(tetop-lacZ)2Mam/J
006004   B6C3-Tg(tetO-APPSwInd)885Dbo/J
006244   C.Cg-Tg(tetO-cre)1Jaw/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
006618   C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
008278   C57BL/6J-Tg(tetO-Clock)1Jt/J
010578   FVB-Tg(tetO-Dusp6)1Jmol/J
008685   FVB-Tg(tetO-Kdr*)4377.5Rwng/J
006439   FVB-Tg(tetO/CMV-KRAS*G12C)9.1Msmi/J
008244   FVB.Cg-Tg(tetO-cre)1Jaw/J
005941   FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J
006202   FVB/N-Tg(tetO-BCR/ABL1)2Dgt/J
003315   FVB/N-Tg(tetORo1-lacZ)3Conk/J
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
006999   STOCK Dbttm1Geh Tg(tTALap)5Bjd Tg(tetO-DBT)A1Geh/J
008755   STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J
008790   STOCK Tg(tetO-DISC1*)1001Plet/J
008168   STOCK Tg(tetO-DTA)1Gfi/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
005728   STOCK Tg(tetO-Ipf1,lacZ)958.1Macd/J
006224   STOCK Tg(tetO-cre)1Jaw/J
View Strains carrying other alleles of tetO     (36 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Tg(tetO-MET)23Rwng/0 Tg(tTALap)5Bjd/0

        involves: FVB/N * NMRI
  • life span-post-weaning/aging
  • premature death (MGI Ref ID J:69731)
    • mice from 2 lines (1 and 2) of double transgenic founders die within 2 months postpartum; early mortality can be prevented by repression of MET expression with doxycycline administration to mating parents, and then to pups until 4 weeks of age
    • with this doxycycline treatment, animals appear normal until 10 months of age, then mice start dying
    • mice from 2 other lines (3 and 4) of double transgenic parents are healthy at birth, then begin to die at 4 months of age
  • growth/size phenotype
  • cachexia (MGI Ref ID J:69731)
    • animals dying without evidence of tumors are usually cachexic
  • liver/biliary system phenotype
  • abnormal hepatocyte morphology (MGI Ref ID J:69731)
    • hepatocytes in foci of hyperplasia develop foamy cytoplasm containing fat deposits
    • with progression of hyperplastic foci toward malignancy, cytologic changes are observed such as cellular and nuclear enlargement with disorganization of cell plate and lobular structures; malignant foci enlarge and develop trabeculae lined with endothelial cells, often separated from each other by blood-filled spaces
  • enlarged liver (MGI Ref ID J:69731)
    • pups are born with enlarged and fatty livers
  • focal hepatic necrosis (MGI Ref ID J:69731)
    • fully developed tumors often display areas of necrosis
  • hepatic steatosis (MGI Ref ID J:69731)
    • pups are born with enlarged and fatty livers
  • jaundice (MGI Ref ID J:69731)
    • animals dying without evidence of tumors display jaundice
  • tumorigenesis
  • hepatocellular carcinoma (MGI Ref ID J:69731)
    • 85% of animals dying after 10 months after receiving DOX till 4 weeks of age show hepatocellular carcinoma (HCC); incidence by 1 year is >60% in lines 1 and 2
    • for lines 3 and 4, 85% of deaths are accompanied by HCC, which appears as an abdominal mass at 6 months of age or later; incidence by 1 year is 60%
    • by 60 weeks of age, many small foci of hyperplasia are detected, often around central hepatic lobule and become more numerous, larger, and uniformly distributed by 4 months of age
    • zones of progression to malignancy are observed within hyperplasias by 6 months, with cells in the zones showing poor organization and less differentiation than normal livers
    • majority of moribund mice treated with doxycycline regain health tumors nearly regress completely, with liver morphology becoming almost normal by 4 months of treatment
    • fully developed tumors often display areas of necrosis
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cancer Research
      Tetop Tet System
Tet Expression Systems
      tTA/rtTA Responsive Strains

MET related

Cancer Research
Increased Tumor Incidence
      Mammary Gland Tumors

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(tetO-MET)23Rwng
Allele Name transgene insertion 23, Rong Wang
Allele Type Transgenic (random, expressed)
Common Name(s) TRE-MET; TRE-hMET;
Mutation Made By Christin Munkittrick,  
Strain of OriginFVB/N
Expressed Gene MET, met proto-oncogene (hepatocyte growth factor receptor), human
Promoter tetO, tet operator,
Molecular Note The TRE-MET transgene was designed with the human MET (met proto-oncogene (hepatocyte growth factor receptor)) coding sequence, under the control of heptamerized tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) sequences and a cytomegalovirus (hCMV) minimal promoter with a beta-globin poly A signal. Founder line 23 was subsequently established. [MGI Ref ID J:69731]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(tetO-MET)23Rwng, Standard PCR
Tg(tetO-MET), Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Wang R; Ferrell LD; Faouzi S; Maher JJ; Bishop JM. 2001. Activation of the met receptor by cell attachment induces and sustains hepatocellular carcinomas in transgenic mice. J Cell Biol 153(5):1023-34. [PubMed: 11381087]  [MGI Ref ID J:69731]

Additional References

Tg(tetO-MET)23Rwng related

Tward AD; Jones KD; Yant S; Cheung ST; Fan ST; Chen X; Kay MA; Wang R; Bishop JM. 2007. Distinct pathways of genomic progression to benign and malignant tumors of the liver. Proc Natl Acad Sci U S A 104(37):14771-14776. [PubMed: 17785413]  [MGI Ref ID J:124960]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           MGL375/MGL377

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these founder line 23 mice may be bred as homozygotes.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   24-FEB-09
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $243.50Female or MaleHemizygous for Tg(tetO-MET)23Rwng
Pairs /Price (US dollars $)Pair Genotype
$297.85Hemizygous for Tg(tetO-MET)23Rwng x Noncarrier
$297.85Noncarrier x Hemizygous for Tg(tetO-MET)23Rwng

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $316.60Female or MaleHemizygous for Tg(tetO-MET)23Rwng
Pairs /Price (US dollars $)Pair Genotype
$387.30Hemizygous for Tg(tetO-MET)23Rwng x Noncarrier
$387.30Noncarrier x Hemizygous for Tg(tetO-MET)23Rwng

Additional Supply Details

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:207-288-6470
fax:207-288-6655

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(3.12)