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| These TRE-MET transgenic mice express human MET (met proto-oncogene (hepatocyte growth factor receptor)) gene under the control of a tetracycline-responsive promoter element (TRE or tetO). When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein or tetracycline-controlled transactivator protein, a bitransgenic animal can be produced that has tissue-specific expression of MET that can be regulated with the tetracycline analog, doxycycline. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?pN1
Generation DefinitionsDonating Investigator Rong Wang, Univ of California Medical Center (UCSF) Description
These transgenic mice express the human MET (met proto-oncogene (hepatocyte growth factor receptor)) gene under the control of a tetracycline-responsive promoter element (TRE or tetO). When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), a bitransgenic animal can be produced that has tissue-specific expression of MET that can be regulated with the tetracycline analog, doxycycline. When bred to mice carrying the transgene LAP-tTA, (see Stock No. 003563 for example), the resulting bi-transgenic mice have inducible overexpression of MET in hepatocytes and represent an effective tool for studying hepatocellular carcinoma.Development
The TRE-MET transgene was designed with the human MET (met proto-oncogene (hepatocyte growth factor receptor)) coding sequence, under the control of heptamerized tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) sequences and a cytomegalovirus (hCMV) minimal promoter with a beta-globin poly A signal. This transgene was injected into fertilized FVB/N mouse oocytes. Founder line 23 was subsequently established.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of MET
002675 FVB/N-Tg(MtTPRMET)243Lng/J 002775 FVB/N-Tg(MtTPRMET)773Lng/J View Strains carrying other alleles of MET (2 strains)
Additional Web Information
Tet Expression Systems
Use of MICE by companies or for-profit entities requires a license prior to shipping.
Use of MICE by companies or for-profit entities requires a license prior to shipping.
Phenotype
Phenotype Information
- Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.
Hepatocellular Carcinoma
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Autism, Susceptibility to, 9; AUTS9 (MET)
Renal Cell Carcinoma, Papillary, 1; RCCP1 (MET)
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Tg(tetO-MET)23Rwng/0 Tg(Cebpb-tTA)5Bjd/0
involves: FVB/N * NMRI
- mortality/aging
- premature death
- mice from 2 lines (1 and 2) of double transgenic founders die within 2 months postpartum; early mortality can be prevented by repression of MET expression with doxycycline administration to mating parents, and then to pups until 4 weeks of age (MGI Ref ID J:69731)
- with this doxycycline treatment, animals appear normal until 10 months of age, then mice start dying (MGI Ref ID J:69731)
- mice from 2 other lines (3 and 4) of double transgenic parents are healthy at birth, then begin to die at 4 months of age (MGI Ref ID J:69731)
- growth/size phenotype
- cachexia
- animals dying without evidence of tumors are usually cachexic (MGI Ref ID J:69731)
- liver/biliary system phenotype
- abnormal hepatocyte morphology
- hepatocytes in foci of hyperplasia develop foamy cytoplasm containing fat deposits (MGI Ref ID J:69731)
- with progression of hyperplastic foci toward malignancy, cytologic changes are observed such as cellular and nuclear enlargement with disorganization of cell plate and lobular structures; malignant foci enlarge and develop trabeculae lined with endothelial cells, often separated from each other by blood-filled spaces (MGI Ref ID J:69731)
- enlarged liver
- pups are born with enlarged and fatty livers (MGI Ref ID J:69731)
- focal hepatic necrosis
- fully developed tumors often display areas of necrosis (MGI Ref ID J:69731)
- hepatic steatosis
- pups are born with enlarged and fatty livers (MGI Ref ID J:69731)
- jaundice
- animals dying without evidence of tumors display jaundice (MGI Ref ID J:69731)
- tumorigenesis
- hepatocellular carcinoma
- 85% of animals dying after 10 months after receiving DOX till 4 weeks of age show hepatocellular carcinoma (HCC); incidence by 1 year is >60% in lines 1 and 2 (MGI Ref ID J:69731)
- for lines 3 and 4, 85% of deaths are accompanied by HCC, which appears as an abdominal mass at 6 months of age or later; incidence by 1 year is 60% (MGI Ref ID J:69731)
- by 60 weeks of age, many small foci of hyperplasia are detected, often around central hepatic lobule and become more numerous, larger, and uniformly distributed by 4 months of age (MGI Ref ID J:69731)
- zones of progression to malignancy are observed within hyperplasias by 6 months, with cells in the zones showing poor organization and less differentiation than normal livers (MGI Ref ID J:69731)
- majority of moribund mice treated with doxycycline regain health tumors nearly regress completely, with liver morphology becoming almost normal by 4 months of treatment (MGI Ref ID J:69731)
- fully developed tumors often display areas of necrosis (MGI Ref ID J:69731)
This mouse can be used to support research in many areas including:
MET relatedCancer Research
Increased Tumor Incidence
Hepatomas
Hepatomas: hepatacellular carcinoma
Research Tools
Cancer Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Responsive Strains
Cancer Research
Increased Tumor Incidence
Mammary Gland Tumors
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(tetO-MET)23Rwng | ||
|---|---|---|---|
| Allele Name | transgene insertion 23, Rong Wang | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | TRE-MET; TRE-hMET; | ||
| Mutation Made By | Christin Munkittrick, | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | MET, met proto-oncogene (hepatocyte growth factor receptor), human | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | The TRE-MET transgene was designed with the human MET (met proto-oncogene (hepatocyte growth factor receptor)) coding sequence, under the control of heptamerized tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) sequences and a cytomegalovirus (hCMV) minimal promoter with a beta-globin poly A signal. Founder line 23 was subsequently established. [MGI Ref ID J:69731] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tetO-MET)23Rwng, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Wang R; Ferrell LD; Faouzi S; Maher JJ; Bishop JM. 2001. Activation of the met receptor by cell attachment induces and sustains hepatocellular carcinomas in transgenic mice. J Cell Biol 153(5):1023-34. [PubMed: 11381087] [MGI Ref ID J:69731]
Additional References
Tg(tetO-MET)23Rwng relatedDamsky WE; Curley DP; Santhanakrishnan M; Rosenbaum LE; Platt JT; Gould Rothberg BE; Taketo MM; Dankort D; Rimm DL; McMahon M; Bosenberg M. 2011. beta-Catenin Signaling Controls Metastasis in Braf-Activated Pten-Deficient Melanomas. Cancer Cell 20(6):741-54. [PubMed: 22172720] [MGI Ref ID J:178598]
Ivanovska I; Zhang C; Liu AM; Wong KF; Lee NP; Lewis P; Philippar U; Bansal D; Buser C; Scott M; Mao M; Poon RT; Fan ST; Cleary MA; Luk JM; Dai H. 2011. Gene signatures derived from a c-MET-driven liver cancer mouse model predict survival of patients with hepatocellular carcinoma. PLoS One 6(9):e24582. [PubMed: 21949730] [MGI Ref ID J:177679]
Tward AD; Jones KD; Yant S; Cheung ST; Fan ST; Chen X; Kay MA; Wang R; Bishop JM. 2007. Distinct pathways of genomic progression to benign and malignant tumors of the liver. Proc Natl Acad Sci U S A 104(37):14771-14776. [PubMed: 17785413] [MGI Ref ID J:124960]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these founder line 23 mice may be bred as homozygotes.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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