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| These IRG transgenic mice are a double-fluorescent, Cre-reporter strain; with widespread expression of red fluorescence prior to Cre recombinase exposure, and green fluorescence following cre-mediated recombination in a pattern determined by cre expression, and should provide a versatile tool for analyzing complex cellular relationships in a wide variety of tissues. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Hemizygote x Noncarrier (Female x Male) 15-JUL-10 Species laboratory mouse Generation N10 (14-JUL-10)
Generation DefinitionsDonating Investigator IMR Colony, The Jackson Laboratory Description
Hemizygous IRG transgenic mice are viable and fertile, with widespread expression of a loxP-flanked optimized red fluorescent protein variant (DsRed-Express) directed to embryonic and adult tissues by the CAG promoter prior to exposure to Cre recombinase. When bred to cre-expressing mice, the resulting offspring have the DsRed-Express cassette deleted in the cre-expressing tissue(s), allowing expression of the enhanced green fluorescent protein (EGFP) cassette located just downstream. These IRG transgenic mice are a double-fluorescent, Cre-reporter strain; with widespread expression of red fluorescence prior to Cre recombinase exposure, and green fluorescence following cre-mediated recombination in a pattern determined by cre expression, and should provide a versatile tool for analyzing complex cellular relationships in a wide variety of tissues.For example, when using IRG transgenic mice along with Nestin-Cre mice (see Stock No. 003771), red and green native fluorescence can be imaged simultaneously in thick tissue sections by confocal microscopy; thus allowing for analysis of complex reconstructions suitable for studying neuronal morphologies as well as neurovascular interactions in brain.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
The CCAG-R/G expression vector was designed with (from 5' to 3') a Human cytomegalovirus immediate early enhancer and chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP site, a DsRed-Express protein sequence followed by three SV40 polyadenylation signals (DsRed-Express (Clontech) is a red fluorescent protein tetramer with improved solubility and fluorescence detection), a second loxP site, an enhanced green fluorescent protein (EGFP) sequence itself followed by a rabbit beta-globin polyadenylation signal. To minimize integration site effects and possible promoter interference, the CCAG-R/S expression vector was flanked on both sides with two paired copies of anti-sense oriented 5'-HS4 insulator sequences from the chicken beta-globin gene; generating the final "insulator/red/green" targeting vector (pIRG). The donating investigator confirms that this 9.4 kb transgene was injected into fertilized oocytes from B6C3F1 hybrid mice. Founder mice were bred to C57BL/6 wildtype mice to generate IRG line 5 (estimated to contain seven copies of the transgene). These IRG mice were maintained on a mixed C57BL/6 and C3H genetic background by breeding hemizygotes with C57BL/6 or B6C3 hybrid mice for many generations prior to arrival at The Jackson Laboratory (as Stock No. 008605). Upon arrival, some mice were additionally backcrossed to C57BL/6J inbred mice (Stock No. 000664) to generate this congenic strain (Stock No. 008705).
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (345 strains)
008605 B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J
008605 B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(CAG-DsRed,-EGFP)5Gae/0
involves: C3H * C57BL/6
- no phenotypic analysis
- *normal* no phenotypic analysis
- hemizygous mice are viable and fertile; no detailed phenotypic is provided (MGI Ref ID J:137251)
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Fluorescent protein expression in neural tissue
Research Tools
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: multiple
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CAG-DsRed,-EGFP)5Gae | ||
|---|---|---|---|
| Allele Name | transgene insertion 5, Gregory A Elder | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | IRG; insulator/red/green; | ||
| Mutation Made By | Gregory Elder, James J. Peters VA Medical Center | ||
| Strain of Origin | (C57BL/6 x C3H)F1 | ||
| Site of Expression | Widespread expression of red fluorescent protein is observed; following cre-mediated recombination, GFP is expressed. | ||
| Expressed Gene | DsRed, red fluorescent protein, | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | The CCAG-R/G expression vector was designed with (from 5' to 3') a Human cytomegalovirus immediate early enhancer and chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP site, a DsRed-Express protein sequence followed by three SV40 polyadenylation signals (DsRed-Express (Clontech) is a red fluorescent protein tetramer with improved solubility and fluorescence detection), a second loxP site, an enhanced green fluorescent protein (EGFP) sequence itself followed by a rabbit beta-globin polyadenylation signal. To minimize integration site effects and possible promoter interference, the CCAG-R/G expression vector was flanked on both sides with two paired copies of anti-sense oriented 5'-HS4 insulator sequences from the chicken beta-globin gene; generating the final "insulator/red/green" targeting vector (pIRG). Line 5 was established and has an estimated transgene copy number of 7. Embryonic and adult tissues exhibit widespread red fluorescent protein expression in the absence of cre recombinase. [MGI Ref ID J:137251] | ||
| Gene Symbol and Name | Tg(CAG-DsRed,-EGFP)5Gae, transgene insertion 5, Gregory A Elder | ||
| Chromosome | UN | ||
| Gene Common Name(s) | IRG; insulator/red/green; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic DsRed, Melt Curve Analysis
Generic DsRed, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
De Gasperi R; Rocher AB; Sosa MA; Wearne SL; Perez GM; Friedrich VL Jr; Hof PR; Elder GA. 2008. The IRG mouse: a two-color fluorescent reporter for assessing Cre-mediated recombination and imaging complex cellular relationships in situ. Genesis 46(6):308-17. [PubMed: 18543298] [MGI Ref ID J:137251]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375
Colony Maintenance
Breeding & Husbandry Mutant mice were bred to C57BL/6J mice for many generations to establish this congenic strain. When maintaining the live congenic colony, hemizygous mice are bred with noncarrier (wildtype) siblings or to C57BL/6J inbred mice. Mating System Hemizygote x Noncarrier (Female x Male) 15-JUL-10 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(CAG-DsRed,-EGFP)5Gae
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(CAG-DsRed,-EGFP)5Gae x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(CAG-DsRed,-EGFP)5Gae Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(CAG-DsRed,-EGFP)5Gae
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(CAG-DsRed,-EGFP)5Gae x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(CAG-DsRed,-EGFP)5Gae Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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