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| These Ple185-EGFP;mEMS1627 mice have the Ple185-EGFP transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) locus on the X chromosome. As the promoter/regulatory regions of the human S100 calcium binding protein B (S100B) gene direct expression of enhanced green fluorescent protein (EGFP), these Ple185-EGFP;mEMS1627 mice may be useful in studying S100β-expressing cells in the brain and diseases affecting the brain. | |||||||||||||||||||
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Description
Strain Information
Former Names B6.129P2-Hprt1tm7(S100B-EGFP)Ems/J (Changed: 10-MAR-09 ) Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Hemizygote (Female x Male) 19-AUG-09 Species laboratory mouse Generation N5+F1 (18-NOV-09) Donating Investigator Elizabeth Simpson, University of British Columbia Description
These Ple185-EGFP;mEMS1627 mice have the Ple185-EGFP transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human S100 calcium binding protein B (S100B) gene directing expression of enhanced green fluorescent protein (EGFP). These Ple185-EGFP;mEMS1627 mice may be useful in studying S100β-expressing cells in the brain and diseases affecting the brain.The donating investigator reports:
The pattern of expression of the Ple185-EGFP MiniPromoter is broad throughout the brain in glial populations, most notably in cerebellar Bergman glia, as well as fiber tracts throughout the brain. Descending white matter tracts coursing through the corpus callosum, striatum, and into the brainstem are also prominently labeled. The EGFP-immunoreactivity shows significant overlap with S100β expression in many regions, including the cerebellum, cortex, and spinal cord. There is also extensive colabeling of EGFP with the myelin marker RIP in white matter tracts throughout the Ple185-EGFP MiniPromoter brain.For more characterization information, see The Pleiades Promoter Project website (Ple185 Promoter (pEMS1384)).
These mice were created and deposited by The Pleiades Promoter Project (Centre for Molecular Medicine and Therapeutics, University of British Columbia); their goal is to generate 160 fully characterized, human DNA promoters of less than 4 kb (MiniPromoters) to drive gene expression in defined brain regions of therapeutic interest for studying disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease, Amyotrophic Lateral Sclerosis (Lou Gehrig's disease), Multiple Sclerosis, Spinocerebellar Ataxia, Depression, Autism, and Cancer.
Development
The Ple185-EGFP transgene (pEMS1384) was designed with a Ple185 minipromoter (S100β-C; a 2440 bp promoter segment and 1340 bp basal promoter element, both from the human S100 calcium binding protein B (S100B) promoter region) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt1b-m3 mutant locus on the X chromosome via electroporation into mEMS1204 embryonic stem cells (generated from F1 generation male pups born of C57BL/6-congenic females homozygous for a Hprt1b-m3 mutation [originally derived from 129P2/OlaHsd-derived E14TG2a embryonic stem cells] X 129S-Gt(ROSA)26Sortm1Sor heterozygous males (see Stock No. 002171 and 003310, respectively)). Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric males (founder line mEMS1627) were bred to B6(Cg)-Tyrc-2J/J females (B6-albino; Stock No. 000058). Female progeny (obligate Hprt1Ple185-EGFP;mEMS1627 heterozygotes) also harboring a Tyr+ allele in combination with the Aw (agouti, white belly) and a (non-agouti/black) coat color alleles were identified and bred with C57BL/6J inbred males (Stock No. 000664). Next, hemizygous Hprt1Ple185-EGFP;mEMS1627 mice were bred with C57BL/6J inbred mice for at least three generations prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred with C57BL/6J inbred mice for at least one generation to establish the live colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
004302 129S1-Hprt1tm1(cre)Mnn/J 002027 129S8/SvEv-Gpi1c Hprt1b-m2/J 002171 B6.129P2-Hprt1b-m3/J 008710 B6.129P2-Hprt1tm10(Ple162-EGFP/cre)Ems/J 008877 B6.129P2-Hprt1tm12(Ple177-EGFP/cre)Ems/J 008706 B6.129P2-Hprt1tm4(Ple88-EGFP)Ems/J 008708 B6.129P2-Hprt1tm8(Ple151-EGFP)Ems/J 008709 B6.129P2-Hprt1tm9(Ple178-EGFP/cre)Ems/J 008876 B6;129-Hprt1tm11(Ple176-EGFP/cre)Ems/J 003138 B6;129-Hprt1tm1Detl/J 000807 RBJ/DnJ
005621 B6;D2-Tg(S100B-EGFP)1Wjt/J 005620 B6;D2-Tg(S100B-EYFP)1Wjt/J 003198 STOCK Tg(huS100B)5Daoh/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: astrocytes
Tissue/Cell Markers: astrocytes, neurons
Tissue/Cell Markers: glial cells
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Hprt1tm7(Ple185-EGFP)Ems | ||
|---|---|---|---|
| Allele Name | targeted mutation 7, Elizabeth M Simpson | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Hprt1tm7(Ple185-EGFP;mEMS1627)Ems; Hprt1tm7(mEMS1627)Ems; Ple185;S100B-C;mEMS1627; | ||
| Mutation Made By | Elizabeth Simpson, University of British Columbia | ||
| Strain of Origin | (B6.129P2-Hprt1 | ||
| ES Cell Line Name | mEMS1204 | ||
| ES Cell Line Strain | (B6.129P2-Hprt1 | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | S100B, S100 calcium binding protein B, human | ||
| Gene Symbol and Name | Hprt1, hypoxanthine guanine phosphoribosyl transferase 1 | ||
| Chromosome | X | ||
| Gene Common Name(s) | C81579; HGPRT; HPGRT; HPRT; Hgprtase; MGC112554; expressed sequence C81579; | ||
| Molecular Note |
The Ple185-EGFP transgene (pEMS1384) was designed with a Ple185 minipromoter (S100beta-C; a 2440 bp promoter segment and 1340 bp basal promoter element, both from the human S100 calcium binding protein B (S100B) promoter region) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt1b-m3 mutant locus on the X chromosome. The promoter/regulatory regions of the human S100 calcium binding protein B (S100B) gene directs expression of enhanced green fluorescent protein (EGFP) to neural white matter glia and astrocytes. There is broad EGFP expression throughout the brain in some glial populations, most notably in cerebellar Bergmann glia, as well as fiber tracts throughout the brain. Descending white matterfiber tracts coursing through the corpus callosum, striatum, and into the brainstem are also prominently labeled. The EGFP expression pattern in the brain co-labels with myelinated axons in the cortex, striatum, and brainstem. [MGI Ref ID J:144244] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1sor STD, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Yang GS; Banks KG; Bonaguro RJ; Wilson G; Dreolini L; de Leeuw CN; Liu L; Swanson DJ; Goldowitz D; Holt RA; Simpson EM. 2008. Next generation tools for high-throughput promoter and expression analysis employing single-copy knock-ins at the Hprt1 locus. Genomics :. [PubMed: 18950699] [MGI Ref ID J:144244]
Additional References
Hprt1tm7(Ple185-EGFP)Ems relatedThe Pleiades Promoter Project, URL: http://pleiades.org/index.php. 2009. Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain MGI Direct Data Submission :. [MGI Ref ID J:145689]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males (Stock No. 000664). Mating System Heterozygote x Hemizygote (Female x Male) 19-AUG-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 23-NOV-09
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Male Hemizygous for Hprt1tm7(Ple185-EGFP)Ems $243.50 Female Heterozygous for Hprt1tm7(Ple185-EGFP)Ems
Pairs /Price (US dollars $) Pair Genotype $487.00 Heterozygous for Hprt1tm7(Ple185-EGFP)Ems x Hemizygous for Hprt1tm7(Ple185-EGFP)Ems
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Male Hemizygous for Hprt1tm7(Ple185-EGFP)Ems $316.60 Female Heterozygous for Hprt1tm7(Ple185-EGFP)Ems
Pairs /Price (US dollars $) Pair Genotype $633.10 Heterozygous for Hprt1tm7(Ple185-EGFP)Ems x Hemizygous for Hprt1tm7(Ple185-EGFP)Ems
Additional Supply Details
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Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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