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| Mouse strains created by the Pleiades Promoter Project (Dr. Elizabeth M. Simpson, Centre for Molecular Medicine and Therapeutics, University of British Columbia, Canada) are now available through the Mutant Mouse Regional Resource Center (MMRRC) at The Jackson Laboratory. For the Ple151-EGFP mice, please see MMRRC #032920 at the MMRRC website. | ||||||||||||||||||||||
| These Ple151-EGFP;mEMS1615 mice have the Ple151-EGFP transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. As the promoter/regulatory regions of the human oligodendrocyte transcription factor 1 (OLIG1) gene direct expression of enhanced green fluorescent protein (EGFP), these Ple151-EGFP;mEMS1615 mice may be useful in studying OLIG1-expressing cells in the brain and diseases affecting the brain. | ||||||||||||||||||||||
- Description
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Description
Strain Information
Former Names B6.129P2-Hprttm8(Ple151-EGFP)Ems/J (Changed: 19-OCT-10 ) B6.129P2-Hprt1tm8(Ple151-EGFP)Ems/J (Changed: 14-DEC-09 ) B6.129P2-Hprt1tm8(OLIG1-EGFP)Ems/J (Changed: 10-MAR-09 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Hemizygote (Female x Male) 30-MAR-10 Species laboratory mouse Generation N5+N2F2pN1
Generation DefinitionsDonating Investigator Elizabeth Simpson, University of British Columbia Description
These Ple151-EGFP;mEMS1615 mice have the Ple151-EGFP transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human oligodendrocyte transcription factor 1 (OLIG1) gene directing expression of enhanced green fluorescent protein (EGFP). These Ple151-EGFP;mEMS1615 mice may be useful in studying OLIG1-expressing cells in the brain and diseases affecting the brain.The donating investigator reports:
Positive EGFP immunoreactivity was detected across numerous brain regions. EGFP-positive cells show darker staining in the nucleus with a lighter staining halo of processes. In the brainstem, cortex and thalamus, numerous immunopositive profiles are detected while fewer profiles are seen in the olfactory bulb, striatum and cerebellum. In contrast to the predicted expression of OLIG1, the EGFP expression does not appear to be enriched in white matter tracts such as the corpus callosum.For more characterization information, see The Pleiades Promoter Project website (Ple151 Promoter (pEMS1172)).
These mice were created and deposited by The Pleiades Promoter Project (Centre for Molecular Medicine and Therapeutics, University of British Columbia); their goal is to generate 160 fully characterized, human DNA promoters of less than 4 kb (MiniPromoters) to drive gene expression in defined brain regions of therapeutic interest for studying disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease, Amyotrophic Lateral Sclerosis (Lou Gehrig's disease), Multiple Sclerosis, Spinocerebellar Ataxia, Depression, Autism, and Cancer.
Development
The Ple151-EGFP transgene (pEMS1172) was designed with a Ple151 minipromoter (OLIG1-D; a combination of four regulatory sequences from the human oligodendrocyte transcription factor 1 (OLIG1) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome via electroporation into mEMS1204 embryonic stem cells (generated from F1 generation male pups born of C57BL/6-congenic females homozygous for a Hprtb-m3 mutation [originally derived from 129P2/OlaHsd-derived E14TG2a embryonic stem cells] X 129S-Gt(ROSA)26Sortm1Sor heterozygous males (see Stock No. 002171 and 003310, respectively)). Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric males (founder line mEMS1615) were bred to B6(Cg)-Tyrc-2J/J females (B6-albino; Stock No. 000058). Female progeny (obligate HprtPle151-EGFP;mEMS1615 heterozygotes) also harboring a Tyr+ allele in combination with the Aw (agouti, white belly) and a (non-agouti/black) coat color alleles were identified and bred with C57BL/6J inbred males (Stock No. 000664). Next, hemizygous HprtPle151-EGFP;mEMS1615 mice were bred with C57BL/6J inbred mice for at least three generations prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred with C57BL/6J inbred mice for at least one generation to establish the live colony.For more information, see The Pleiades Promoter Project website (Ple151 Promoter (pEMS1172)).
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
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Additional Web Information
Fluorescent Proteins/lacZ Systems
JAX® NOTES, Spring 2009; 513. Pleiades Promoter Project mice shine at The Jackson Laboratory.
Phenotype
Phenotype Information
assigned by genotype
Hprttm8(Ple151-EGFP)Ems/Y
B6.129P2(Cg)-Hprttm8(Ple151-EGFP)Ems/Mmjax
- normal phenotype
- no abnormal phenotype detected
- heterozygous females and hemizygous males are viable and fertile (MGI Ref ID J:145689)
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: glial cells
Tissue/Cell Markers: glial cells, oligodendrocytes, Schwann cells
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Hprttm8(Ple151-EGFP)Ems | ||
|---|---|---|---|
| Allele Name | targeted mutation 8, Elizabeth M Simpson | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Hprt1tm8(OLIG1-EGFP)Ems; Hprt1tm8(Ple151-EGFP;mEMS1615)Ems; Hprt1tm8(mEMS1615)Ems; Ple151;OLIG1-D;mEMS1615; | ||
| Mutation Made By | Elizabeth Simpson, University of British Columbia | ||
| Strain of Origin | (B6.129P2-Hprt | ||
| ES Cell Line Name | mEMS1204 | ||
| ES Cell Line Strain | (B6.129P2-Hprt | ||
| Site of Expression | EGFP immunoreactivity is reported across numerous brain regions including the brainstem, cortex and thalamus. Less is detected in the olfactory bulb, striatum and cerebellum. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | OLIG1, oligodendrocyte transcription factor 1, human | ||
| Associated Marker Note | Expressed-count: 1Ex1-Source: JellyfishEx1-Gene: EGFPDriver-count: 1Dr1-Source: HumanDr1-Gene: 116448 | ||
| General Note | Germ line transmission of mutant cell line mEMS1615 has been established. | ||
| Molecular Note |
The Ple151-EGFP transgene (pEMS1172) was designed with a Ple151 minipromoter (OLIG1-D; a combination of four regulatory sequences from the human oligodendrocyte transcription factor 1 (OLIG1) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct wastargeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. The promoter/regulatory regions of the human oligodendrocyte transcription factor 1 (OLIG1) gene direct expression of enhanced green fluorescent protein (EGFP) to neural white matter glia and oligodendroglia. EGFP expression (via anti-GFP immunocytochemistry) is observed throughout cortical and subcortical regions of the brain. Within the cortex, individual cell bodies are distinctly labeled and "puffy" processes surround these cells. In contrast to the predicted expression of OLIG1, EGFP expression does not appear to be enriched in white matter tracts or in the corpus callosum. [MGI Ref ID J:144244] [MGI Ref ID J:164356] | ||
| Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase | ||
| Chromosome | X | ||
| Gene Common Name(s) | C81579; HGPRT; Hgprtase; Hprt1; expressed sequence C81579; hypoxanthine guanine phosphoribosyl transferase 1; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1sor STD, Robotic STD
Gt(ROSA)26Sortm1sor STD, Standard PCR
Hprt1tm8(Ple151-EGFP)Ems, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Yang GS; Banks KG; Bonaguro RJ; Wilson G; Dreolini L; de Leeuw CN; Liu L; Swanson DJ; Goldowitz D; Holt RA; Simpson EM. 2009. Next generation tools for high-throughput promoter and expression analysis employing single-copy knock-ins at the Hprt1 locus. Genomics 93(3):196-204. [PubMed: 18950699] [MGI Ref ID J:144244]
Additional References
Hprttm8(Ple151-EGFP)Ems relatedPortales-Casamar E; Swanson DJ; Liu L; de Leeuw CN; Banks KG; Ho Sui SJ; Fulton DL; Ali J; Amirabbasi M; Arenillas DJ; Babyak N; Black SF; Bonaguro RJ; Brauer E; Candido TR; Castellarin M; Chen J; Chen Y; Cheng JC; Chopra V; Docking TR; Dreolini L; D'Souza CA; Flynn EK; Glenn R; Hatakka K; Hearty TG; Imanian B; Jiang S; Khorasan-zadeh S; Komljenovic I; Laprise S; Liao NY; Lim JS; Lithwick S; Liu F; Liu J; Lu M; McConechy M; McLeod AJ; Milisavljevic M; Mis J; O'Connor K; Palma B; Palmquist DL; Simpson EM:. 2010. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A 107(38):16589-94. [PubMed: 20807748] [MGI Ref ID J:164356]
The Pleiades Promoter Project. 2009. Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain MGI Direct Data Submission :. [MGI Ref ID J:145689]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry This targeted mutation is on the X chromosome. The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males (Stock No. 000664). Note, homozygous females may also be bred with hemizygous males (as long as there are no reported problems with such a breeding scheme). Mating System Homozygote x Hemizygote (Female x Male) 30-MAR-10
Purchasing information
Pricing, Supply Level & Notes, Controls
This strain is currently Transferred.
General Supply Notes
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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