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| Mouse strains created by the Pleiades Promoter Project (Dr. Elizabeth M. Simpson, Centre for Molecular Medicine and Therapeutics, University of British Columbia, Canada) are now available through the Mutant Mouse Regional Resource Center (MMRRC) at The Jackson Laboratory. For the Ple178-EGFP/cre mice, please see MMRRC #032921 at the MMRRC website. | |||||||||||||||||||
| These Ple178-EGFPCre;mEMS756 mice have the Ple178-EGFPCre transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. As the promoter/regulatory regions of the human regulator of G-protein signaling 16 (RGS16) gene direct expression of an EGFPCre fusion protein to hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum, and retina, these Ple178-EGFPCre;mEMS756 mice may be useful in studying RGS16-expressing cells in the brain and diseases affecting the brain. | |||||||||||||||||||
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- References
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Description
Strain Information
Former Names B6.129P2(Cg)-Hprttm9(Ple178-EGFP/cre)Ems/Mmjax (Changed: 19-OCT-10 ) B6.129P2-Hprttm9(Ple178-EGFP/cre)Ems/J (Changed: 19-OCT-10 ) B6.129P2-Hprt1tm9(Ple178-EGFP/cre)Ems/J (Changed: 14-DEC-09 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N4+N1pN1
Generation DefinitionsDonating Investigator Elizabeth M Simpson, University of British Columbia
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Hprttm9(Ple178-EGFP/cre)Ems/Y
B6.129P2(129S4)-Hprttm9(Ple178-EGFP/cre)Ems
- normal phenotype
- no abnormal phenotype detected
- heterozygous females and hemizygous males are viable and fertile (MGI Ref ID J:145689)
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
cre relatedResearch Tools
Fluorescent Proteins
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Hprttm9(Ple178-EGFP/cre)Ems | ||
|---|---|---|---|
| Allele Name | targeted mutation 9, Elizabeth M Simpson | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Hprt1tm9(Ple178-EGFPcre;mEMS756)Ems; Hprt1tm9(mEMS756)Ems; RGS16-D-EFGP/cre; | ||
| Mutation Made By | Elizabeth Simpson, University of British Columbia | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14TG2a | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | Expression of an EGFPCre fusion protein is directed to hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum, and retina | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | RGS16, regulator of G-protein signaling 16, human | ||
| Driver Note | RGS16 | ||
| General Note | Germ line transmission of mutant cell line mEMS756 has been established. | ||
| Molecular Note | The Ple178-EGFPCre transgene (pEMS1090) was designed with the 864 bp Ple178 minipromoter (RGS16-D; derived from an intronic candidate regulatory region and a subsection of the human regulator of G-protein signaling 16 (RGS16) promoter) upstream of a minimal F5 mutant-frt site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). [MGI Ref ID J:164356] | ||
| Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase | ||
| Chromosome | X | ||
| Gene Common Name(s) | C81579; HGPRT; Hgprtase; Hprt1; expressed sequence C81579; hypoxanthine guanine phosphoribosyl transferase 1; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Hprt1tm9(Ple178-EGFP/cre)Ems STD PCR, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
The Pleiades Promoter Project. 2009. Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain MGI Direct Data Submission :. [MGI Ref ID J:145689]
Additional References
Hprttm9(Ple178-EGFP/cre)Ems relatedPortales-Casamar E; Swanson DJ; Liu L; de Leeuw CN; Banks KG; Ho Sui SJ; Fulton DL; Ali J; Amirabbasi M; Arenillas DJ; Babyak N; Black SF; Bonaguro RJ; Brauer E; Candido TR; Castellarin M; Chen J; Chen Y; Cheng JC; Chopra V; Docking TR; Dreolini L; D'Souza CA; Flynn EK; Glenn R; Hatakka K; Hearty TG; Imanian B; Jiang S; Khorasan-zadeh S; Komljenovic I; Laprise S; Liao NY; Lim JS; Lithwick S; Liu F; Liu J; Lu M; McConechy M; McLeod AJ; Milisavljevic M; Mis J; O'Connor K; Palma B; Palmquist DL; Simpson EM:. 2010. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A 107(38):16589-94. [PubMed: 20807748] [MGI Ref ID J:164356]
Yang GS; Banks KG; Bonaguro RJ; Wilson G; Dreolini L; de Leeuw CN; Liu L; Swanson DJ; Goldowitz D; Holt RA; Simpson EM. 2009. Next generation tools for high-throughput promoter and expression analysis employing single-copy knock-ins at the Hprt1 locus. Genomics 93(3):196-204. [PubMed: 18950699] [MGI Ref ID J:144244]
Health & husbandry
Health & Colony Maintenance Information
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Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
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