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| WAP-Cre transgenic mice have expression of Cre recombinase directed to mammary gland secretory epithelial cells by the mouse Wap (whey acidic protein) promoter, and may be used to generate conditional knockouts for studying breast cancer and lactation (for example). | |||||||||||||||
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- Disease & phenotype
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6.Cg-Tg(Wap-cre)11738Mam/JKwnJ (Changed: 04-NOV-09 ) Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Dr. Barbara Knowles, Institute of Medical Biology Description
WAP-Cre transgenic mice are viable and fertile, with expression of P1 Cre recombinase under the control of the mouse Wap (whey acidic protein) promoter. When bred with other mutant mice containing loxP-flanked sequence(s), Cre-mediated recombination will result in deletion of the floxed sequence(s) in mammary gland epithelium. Deletion of floxed sequences is reported in brain tissue from WAP-Cre mice on a mixed genetic background but has not been examined in this strain. Maximum Cre recombinase activity is achieved during pregnancy and lactation, but in virgin mice small numbers of mammary epithelial cells show evidence of estrus cycle-related Cre recombinase activity. These WAP-Cre transgenic mice may be useful in generating conditional mutations in mammary glands for studying mammary physiology and processes such as breast development and breast cancer.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
The WAP-Cre transgene was designed by Drs. Lothar Hennighausen and Kay-Uwe Wagner (NIH) to contain the 2.6 kb mouse Wap (whey acidic protein) promoter and cre coding sequence, followed by an intron and polyadenylation site from the rabbit beta-globin gene. The donating investigator reports that the transgenic mice were generated on a B6SJLF1/J genetic background. Mice from founder line 11738 were bred to FVB mice before being crossed to B6129F1 mice by the donating investigator. Mice on a mixed genetic background were then sent to The Jackson Laboratory (as Stock No. 003552). After this, some mice were obtained by Dr. Barbara Knowles at The Jackson Laboratory and additionally backcrossed for 10 generations to C57BL/6J inbred mice (Stock No. 000664), resulting in this congenic strain (Stock No. 008735).
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(Wap-cre)11738Mam allele
003552 B6129-Tg(Wap-cre)11738Mam/J View Strains carrying Tg(Wap-cre)11738Mam (1 strain)
010562 B6.Cg-Tg(Wap-ERBB2)229Wzw/J 002409 B6;SJL-Tg(Wap-HRAS)69Lln Chr YSJL/J 003189 C57BL/6J-Tg(WAPTAg)3Knw/J 002500 C57BL/6J-Tg(WapIgf1)39Dlr/J 003188 C57BL/6J-Tg(WapTAg)1Knw/J 002410 FVB.Cg-Tg(Wap-HRAS)69Lln Chr YSJL/J 003078 FVB.Cg-Tg(WapIgf1)39Dlr/J 002678 FVB.Cg-Tg(WapTgfa)215Bri/J 002755 FVB/N-Tg(WapNotch4)10Rnc/J 016116 STOCK Waptm2(rtTA)Kuw/J 004184 STOCK Tg(Wap-HRAS)69Lln Chr YSJL-Edaraddcr-3J/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedCancer Research
Other
Research Tools
Cancer Research
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Reproductive Biology Research
Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Wap-cre)11738Mam | ||
|---|---|---|---|
| Allele Name | transgene insertion 11738, Lothar Hennighausen | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | WAP-Cre; WC; | ||
| Mutation Made By | Dr. Kay-Uwe Wagner, University of Nebraska Medical Center | ||
| Strain of Origin | (C57BL/6 x SJL)F1 | ||
| Site of Expression | mammary gland tissues, specifically the secretory epithelium; not entirely restricted to mammary gland, a limited amount of cre activity has been reported in brain tissue | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Wap, whey acidic protein, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Wap-cre)11738Mam, transgene insertion 11738, Lothar Hennighausen | ||
| Chromosome | UN | ||
| Gene Common Name(s) | WAP-Cre; WC; | ||
| Driver Note | Wap | ||
| Molecular Note | This transgene directs the expression of Cre recombinase under the control of a 2.6 kb (EcoRI-Asp718) mouse whey acidic protein (Wap) promoter fragment. The Wap promoter directed expression of Cre recombinase in the mammary glands of late pregnant and lactating female mice. No expression was detected in mammary tissue of virgin mice. [MGI Ref ID J:67925] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Wagner KU; Wall RJ; St-Onge L; Gruss P; Wynshaw-Boris A; Garrett L; Li M; Furth PA; Hennighausen L. 1997. Cre-mediated gene deletion in the mammary gland. Nucleic Acids Res 25(21):4323-30. [PubMed: 9336464] [MGI Ref ID J:67925]
Additional References
Tg(Wap-cre)11738Mam relatedAhmed F; Wyckoff J; Lin EY; Wang W; Wang Y; Hennighausen L; Miyazaki J; Jones J; Pollard JW; Condeelis JS; Segall JE. 2002. GFP Expression in the Mammary Gland for Imaging of Mammary Tumor Cells in Transgenic Mice. Cancer Res 62(24):7166-9. [PubMed: 12499251] [MGI Ref ID J:80828]
Asselin-Labat ML; Sutherland KD; Barker H; Thomas R; Shackleton M; Forrest NC; Hartley L; Robb L; Grosveld FG; van der Wees J; Lindeman GJ; Visvader JE. 2007. Gata-3 is an essential regulator of mammary-gland morphogenesis and luminal-cell differentiation. Nat Cell Biol 9(2):201-9. [PubMed: 17187062] [MGI Ref ID J:185713]
Bierie B; Stover DG; Abel TW; Chytil A; Gorska AE; Aakre M; Forrester E; Yang L; Wagner KU; Moses HL. 2008. Transforming growth factor-beta regulates mammary carcinoma cell survival and interaction with the adjacent microenvironment. Cancer Res 68(6):1809-19. [PubMed: 18339861] [MGI Ref ID J:133311]
Brodie SG; Xu X; Qiao W; Li WM; Cao L; Deng CX. 2001. Multiple genetic changes are associated with mammary tumorigenesis in Brca1 conditional knockout mice. Oncogene 20(51):7514-23. [PubMed: 11709723] [MGI Ref ID J:72815]
Bry C; Maass K; Miyoshi K; Willecke K; Ott T; Robinson GW; Hennighausen L. 2004. Loss of connexin 26 in mammary epithelium during early but not during late pregnancy results in unscheduled apoptosis and impaired development. Dev Biol 267(2):418-29. [PubMed: 15013803] [MGI Ref ID J:88743]
Carstens MJ; Krempler A; Triplett AA; Van Lohuizen M; Wagner KU. 2004. Cell cycle arrest and cell death are controlled by p53-dependent and p53-independent mechanisms in Tsg101-deficient cells. J Biol Chem 279(34):35984-94. [PubMed: 15210712] [MGI Ref ID J:92528]
Chang MY; Boulden J; Sutanto-Ward E; Duhadaway JB; Soler AP; Muller AJ; Prendergast GC. 2007. Bin1 ablation in mammary gland delays tissue remodeling and drives cancer progression. Cancer Res 67(1):100-7. [PubMed: 17210688] [MGI Ref ID J:117330]
Cheung AM; Elia A; Tsao MS; Done S; Wagner KU; Hennighausen L; Hakem R; Mak TW. 2004. Brca2 deficiency does not impair mammary epithelium development but promotes mammary adenocarcinoma formation in p53(+/-) mutant mice. Cancer Res 64(6):1959-65. [PubMed: 15026330] [MGI Ref ID J:89150]
Cui Y; Miyoshi K; Claudio E; Siebenlist UK; Gonzalez FJ; Flaws J; Wagner KU; Hennighausen L. 2002. Loss of the peroxisome proliferation-activated receptor gamma (PPARgamma ) does not affect mammary development and propensity for tumor formation but leads to reduced fertility. J Biol Chem 277(20):17830-5. [PubMed: 11884400] [MGI Ref ID J:76553]
Cui Y; Riedlinger G; Miyoshi K; Tang W; Li C; Deng CX; Robinson GW; Hennighausen L. 2004. Inactivation of Stat5 in mouse mammary epithelium during pregnancy reveals distinct functions in cell proliferation, survival, and differentiation. Mol Cell Biol 24(18):8037-47. [PubMed: 15340066] [MGI Ref ID J:92770]
Declercq J; Skaland I; Van Dyck F; Janssen EA; Baak JP; Drijkoningen M; Van de Ven WJ. 2008. Adenomyoepitheliomatous lesions of the mammary glands in transgenic mice with targeted PLAG1 overexpression. Int J Cancer 123(7):1593-600. [PubMed: 18649356] [MGI Ref ID J:140057]
Deng CX; Xu X. 2004. Generation and analysis of brca1 conditional knockout mice. Methods Mol Biol 280:185-200. [PubMed: 15187254] [MGI Ref ID J:90788]
Feng Y; Manka D; Wagner KU; Khan SA. 2007. Estrogen receptor-{alpha} expression in the mammary epithelium is required for ductal and alveolar morphogenesis in mice. Proc Natl Acad Sci U S A 104(37):14718-23. [PubMed: 17785410] [MGI Ref ID J:124962]
Frau E; Magnon C; Opolon P; Connault E; Opolon D; Beermann F; Abitbol M; Perricaudet M; Bouquet C. 2007. A gene transfer comparative study of HSA-conjugated antiangiogenic factors in a transgenic mouse model of metastatic ocular cancer. Cancer Gene Ther 14(3):251-61. [PubMed: 17082795] [MGI Ref ID J:133447]
Hesling C; Lopez J; Fattet L; Gonzalo P; Treilleux I; Blanchard D; Losson R; Goffin V; Pigat N; Puisieux A; Mikaelian I; Gillet G; Rimokh R. 2013. Tif1gamma is essential for the terminal differentiation of mammary alveolar epithelial cells and for lactation through SMAD4 inhibition. Development 140(1):167-75. [PubMed: 23154409] [MGI Ref ID J:191080]
Humphreys RC; Bierie B; Zhao L; Raz R; Levy D; Hennighausen L. 2002. Deletion of Stat3 blocks mammary gland involution and extends functional competence of the secretory epithelium in the absence of lactogenic stimuli. Endocrinology 143(9):3641-50. [PubMed: 12193580] [MGI Ref ID J:81459]
Jeselsohn R; Brown NE; Arendt L; Klebba I; Hu MG; Kuperwasser C; Hinds PW. 2010. Cyclin D1 Kinase Activity Is Required for the Self-Renewal of Mammary Stem and Progenitor Cells that Are Targets of MMTV-ErbB2 Tumorigenesis. Cancer Cell 17(1):65-76. [PubMed: 20129248] [MGI Ref ID J:156929]
Jiang Z; Deng T; Jones R; Li H; Herschkowitz JI; Liu JC; Weigman VJ; Tsao MS; Lane TF; Perou CM; Zacksenhaus E. 2010. Rb deletion in mouse mammary progenitors induces luminal-B or basal-like/EMT tumor subtypes depending on p53 status. J Clin Invest 120(9):3296-309. [PubMed: 20679727] [MGI Ref ID J:165289]
Klein L; Khazaie K; von Boehmer H. 2003. In vivo dynamics of antigen-specific regulatory T cells not predicted from behavior in vitro. Proc Natl Acad Sci U S A 100(15):8886-91. [PubMed: 12857946] [MGI Ref ID J:125750]
Klover PJ; Muller WJ; Robinson GW; Pfeiffer RM; Yamaji D; Hennighausen L. 2010. Loss of STAT1 from mouse mammary epithelium results in an increased neu-induced tumor burden. Neoplasia 12(11):899-905. [PubMed: 21076615] [MGI Ref ID J:169262]
Kode A; Mosialou I; Silva BC; Joshi S; Ferron M; Rached MT; Kousteni S. 2012. FoxO1 protein cooperates with ATF4 protein in osteoblasts to control glucose homeostasis. J Biol Chem 287(12):8757-68. [PubMed: 22298775] [MGI Ref ID J:183276]
Kumar P; Mukherjee M; Johnson JP; Patel M; Huey B; Albertson DG; Simin K. 2012. Cooperativity of Rb, Brca1, and p53 in malignant breast cancer evolution. PLoS Genet 8(11):e1003027. [PubMed: 23173005] [MGI Ref ID J:194081]
Kumaraswamy E; Carlson BA; Morgan F; Miyoshi K; Robinson GW; Su D; Wang S; Southon E; Tessarollo L; Lee BJ; Gladyshev VN; Hennighausen L; Hatfield DL. 2003. Selective removal of the selenocysteine tRNA [Ser]Sec gene (Trsp) in mouse mammary epithelium. Mol Cell Biol 23(5):1477-88. [PubMed: 12588969] [MGI Ref ID J:82321]
Kuraguchi M; Ohene-Baah NY; Sonkin D; Bronson RT; Kucherlapati R. 2009. Genetic mechanisms in apc-mediated mammary tumorigenesis. PLoS Genet 5(2):e1000367. [PubMed: 19197353] [MGI Ref ID J:146481]
Lam MH; Liu Q; Elledge SJ; Rosen JM. 2004. Chk1 is haploinsufficient for multiple functions critical to tumor suppression. Cancer Cell 6(1):45-59. [PubMed: 15261141] [MGI Ref ID J:91266]
Le Provost F; Riedlinger G; Hee Yim S; Benedict J; Gonzalez FJ; Flaws J; Hennighausen L. 2002. The aryl hydrocarbon receptor (AhR) and its nuclear translocator (Arnt) are dispensable for normal mammary gland development but are required for fertility. Genesis 32(3):231-9. [PubMed: 11892012] [MGI Ref ID J:76280]
Li W; Qiao W; Chen L; Xu X; Yang X; Li D; Li C; Brodie SG; Meguid MM; Hennighausen L; Deng CX. 2003. Squamous cell carcinoma and mammary abscess formation through squamous metaplasia in Smad4/Dpc4 conditional knockout mice. Development 130(24):6143-53. [PubMed: 14597578] [MGI Ref ID J:86484]
Li W; Xiao C; Vonderhaar BK; Deng CX. 2007. A role of estrogen/ERalpha signaling in BRCA1-associated tissue-specific tumor formation. Oncogene 26(51):7204-12. [PubMed: 17496925] [MGI Ref ID J:129624]
Li Z; Tognon CE; Godinho FJ; Yasaitis L; Hock H; Herschkowitz JI; Lannon CL; Cho E; Kim SJ; Bronson RT; Perou CM; Sorensen PH; Orkin SH. 2007. ETV6-NTRK3 fusion oncogene initiates breast cancer from committed mammary progenitors via activation of AP1 complex. Cancer Cell 12(6):542-58. [PubMed: 18068631] [MGI Ref ID J:130323]
Loladze AV; Stull MA; Rowzee AM; Demarco J; Lantry JH 3rd; Rosen CJ; Leroith D; Wagner KU; Hennighausen L; Wood TL. 2006. Epithelial-specific and stage-specific functions of insulin-like growth factor-I during postnatal mammary development. Endocrinology 147(11):5412-23. [PubMed: 16901968] [MGI Ref ID J:117180]
Long W; Wagner KU; Lloyd KC; Binart N; Shillingford JM; Hennighausen L; Jones FE. 2003. Impaired differentiation and lactational failure of Erbb4-deficient mammary glands identify ERBB4 as an obligate mediator of STAT5. Development 130(21):5257-68. [PubMed: 12954715] [MGI Ref ID J:86319]
Manuylov NL; Smagulova FO; Tevosian SG. 2007. Fog2 excision in mice leads to premature mammary gland involution and reduced Esr1 gene expression Oncogene 26(36):5204-13. [PubMed: 17310981] [MGI Ref ID J:120985]
McPherson JP; Lemmers B; Hirao A; Hakem A; Abraham J; Migon E; Matysiak-Zablocki E; Tamblyn L; Sanchez-Sweatman O; Khokha R; Squire J; Hande MP; Mak TW; Hakem R. 2004. Collaboration of Brca1 and Chk2 in tumorigenesis. Genes Dev 18(10):1144-53. [PubMed: 15131084] [MGI Ref ID J:90512]
Miyoshi K; Shillingford JM; Le Provost F; Gounari F; Bronson R; von Boehmer H; Taketo MM; Cardiff RD; Hennighausen L; Khazaie K. 2002. Activation of beta -catenin signaling in differentiated mammary secretory cells induces transdifferentiation into epidermis and squamous metaplasias. Proc Natl Acad Sci U S A 99(1):219-24. [PubMed: 11773619] [MGI Ref ID J:73556]
Morel AP; Hinkal GW; Thomas C; Fauvet F; Courtois-Cox S; Wierinckx A; Devouassoux-Shisheboran M; Treilleux I; Tissier A; Gras B; Pourchet J; Puisieux I; Browne GJ; Spicer DB; Lachuer J; Ansieau S; Puisieux A. 2012. EMT Inducers Catalyze Malignant Transformation of Mammary Epithelial Cells and Drive Tumorigenesis towards Claudin-Low Tumors in Transgenic Mice. PLoS Genet 8(5):e1002723. [PubMed: 22654675] [MGI Ref ID J:185192]
Morroni M; Giordano A; Zingaretti MC; Boiani R; De Matteis R; Kahn BB; Nisoli E; Tonello C; Pisoschi C; Luchetti MM; Marelli M; Cinti S. 2004. Reversible transdifferentiation of secretory epithelial cells into adipocytes in the mammary gland. Proc Natl Acad Sci U S A 101(48):16801-6. [PubMed: 15556998] [MGI Ref ID J:94729]
Nemade RV; Bierie B; Nozawa M; Bry C; Smith GH; Vasioukhin V; Fuchs E; Hennighausen L. 2004. Biogenesis and function of mouse mammary epithelium depends on the presence of functional alpha-catenin. Mech Dev 121(1):91-9. [PubMed: 14706703] [MGI Ref ID J:87469]
Oh KB; Stanton MJ; West WW; Todd GL; Wagner KU. 2007. Tsg101 is upregulated in a subset of invasive human breast cancers and its targeted overexpression in transgenic mice reveals weak oncogenic properties for mammary cancer initiation. Oncogene 26(40):5950-9. [PubMed: 17369844] [MGI Ref ID J:121475]
Park S; Zhao Y; Yoon S; Xu J; Liao L; Lydon J; DeMayo F; O'Malley BW; Katzenellenbogen BS. 2011. Repressor of estrogen receptor activity (REA) is essential for mammary gland morphogenesis and functional activities: studies in conditional knockout mice. Endocrinology 152(11):4336-49. [PubMed: 21862609] [MGI Ref ID J:178347]
Peddibhotla S; Lam MH; Gonzalez-Rimbau M; Rosen JM. 2009. The DNA-damage effector checkpoint kinase 1 is essential for chromosome segregation and cytokinesis. Proc Natl Acad Sci U S A 106(13):5159-64. [PubMed: 19289837] [MGI Ref ID J:147137]
Raafat A; Lawson S; Bargo S; Klauzinska M; Strizzi L; Goldhar AS; Buono K; Salomon D; Vonderhaar BK; Callahan R. 2009. Rbpj conditional knockout reveals distinct functions of Notch4/Int3 in mammary gland development and tumorigenesis. Oncogene 28(2):219-30. [PubMed: 18836481] [MGI Ref ID J:145788]
Robinson GW; Pacher-Zavisin M; Zhu BM; Yoshimura A; Hennighausen L. 2007. Socs 3 modulates the activity of the transcription factor Stat3 in mammary tissue and controls alveolar homeostasis. Dev Dyn 236(3):654-61. [PubMed: 17205581] [MGI Ref ID J:118349]
Ryu S; McDonnell K; Choi H; Gao D; Hahn M; Joshi N; Park SM; Catena R; Do Y; Brazin J; Vahdat LT; Silver RB; Mittal V. 2013. Suppression of miRNA-708 by polycomb group promotes metastases by calcium-induced cell migration. Cancer Cell 23(1):63-76. [PubMed: 23328481] [MGI Ref ID J:194343]
Sakamoto K; Lin WC; Triplett AA; Wagner KU. 2009. Targeting janus kinase 2 in Her2/neu-expressing mammary cancer: Implications for cancer prevention and therapy. Cancer Res 69(16):6642-50. [PubMed: 19638583] [MGI Ref ID J:151772]
Seagroves TN; Peacock DL; Liao D; Schwab LP; Krueger R; Handorf CR; Haase VH; Johnson RS. 2010. VHL deletion impairs mammary alveologenesis but is not sufficient for mammary tumorigenesis. Am J Pathol 176(5):2269-82. [PubMed: 20382704] [MGI Ref ID J:160375]
Semba S; Han SY; Qin HR; McCorkell KA; Iliopoulos D; Pekarsky Y; Druck T; Trapasso F; Croce CM; Huebner K. 2006. Biological functions of mammalian Nit1, the counterpart of the invertebrate NitFhit Rosetta stone protein, a possible tumor suppressor. J Biol Chem 281(38):28244-53. [PubMed: 16864578] [MGI Ref ID J:115052]
Serber DW; Rogala A; Makarem M; Rosson GB; Simin K; Godfrey V; Van Dyke T; Eaves CJ; Bultman SJ. 2012. The BRG1 chromatin remodeler protects against ovarian cysts, uterine tumors, and mammary tumors in a lineage-specific manner. PLoS One 7(2):e31346. [PubMed: 22363625] [MGI Ref ID J:185316]
Shillingford JM; Miyoshi K; Robinson GW; Bierie B; Cao Y; Karin M; Hennighausen L. 2003. Proteotyping of mammary tissue from transgenic and gene knockout mice with immunohistochemical markers: a tool to define developmental lesions. J Histochem Cytochem 51(5):555-65. [PubMed: 12704203] [MGI Ref ID J:124525]
Thangaraju M; Rudelius M; Bierie B; Raffeld M; Sharan S; Hennighausen L; Huang AM; Sterneck E. 2005. C/EBPdelta is a crucial regulator of pro-apoptotic gene expression during mammary gland involution. Development 132(21):4675-85. [PubMed: 16192306] [MGI Ref ID J:102705]
Trimboli AJ; Fukino K; de Bruin A; Wei G; Shen L; Tanner SM; Creasap N; Rosol TJ; Robinson ML; Eng C; Ostrowski MC; Leone G. 2008. Direct evidence for epithelial-mesenchymal transitions in breast cancer. Cancer Res 68(3):937-45. [PubMed: 18245497] [MGI Ref ID J:131862]
Wagh PK; Zinser GM; Gray JK; Shrestha A; Waltz SE. 2012. Conditional deletion of beta-catenin in mammary epithelial cells of Ron receptor, Mst1r, overexpressing mice alters mammary tumorigenesis. Endocrinology 153(6):2735-46. [PubMed: 22474186] [MGI Ref ID J:186728]
Wagner KU; Boulanger CA; Henry MD; Sgagias M; Hennighausen L; Smith GH. 2002. An adjunct mammary epithelial cell population in parous females: its role in functional adaptation and tissue renewal. Development 129(6):1377-86. [PubMed: 11880347] [MGI Ref ID J:75045]
Wagner KU; Krempler A; Qi Y; Park K; Henry MD; Triplett AA; Riedlinger G; Rucker III EB; Hennighausen L. 2003. Tsg101 is essential for cell growth, proliferation, and cell survival of embryonic and adult tissues. Mol Cell Biol 23(1):150-62. [PubMed: 12482969] [MGI Ref ID J:80891]
Wagner KU; Krempler A; Triplett AA; Qi Y; George NM; Zhu J; Rui H. 2004. Impaired alveologenesis and maintenance of secretory mammary epithelial cells in Jak2 conditional knockout mice. Mol Cell Biol 24(12):5510-20. [PubMed: 15169911] [MGI Ref ID J:90883]
Wang RH; Zheng Y; Kim HS; Xu X; Cao L; Luhasen T; Lee MH; Xiao C; Vassilopoulos A; Chen W; Gardner K; Man YG; Hung MC; Finkel T; Deng CX. 2008. Interplay among BRCA1, SIRT1, and Survivin during BRCA1-associated tumorigenesis. Mol Cell 32(1):11-20. [PubMed: 18851829] [MGI Ref ID J:141108]
Wang X; Cunningham M; Zhang X; Tokarz S; Laraway B; Troxell M; Sears RC. 2011. Phosphorylation regulates c-Myc's oncogenic activity in the mammary gland. Cancer Res 71(3):925-36. [PubMed: 21266350] [MGI Ref ID J:169403]
Weaver Z; Montagna C; Xu X; Howard T; Gadina M; Brodie SG; Deng CX; Ried T. 2002. Mammary tumors in mice conditionally mutant for Brca1 exhibit gross genomic instability and centrosome amplification yet display a recurring distribution of genomic imbalances that is similar to human breast cancer. Oncogene 21(33):5097-107. [PubMed: 12140760] [MGI Ref ID J:77948]
Weiler S; Rohrbach V; Pulvirenti T; Adams R; Ziemiecki A; Andres AC. 2009. Mammary epithelial-specific knockout of the ephrin-B2 gene leads to precocious epithelial cell death at lactation. Dev Growth Differ 51(9):809-19. [PubMed: 19843150] [MGI Ref ID J:166309]
Wijnhoven SW; Zwart E; Speksnijder EN; Beems RB; Olive KP; Tuveson DA; Jonkers J; Schaap MM; van den Berg J; Jacks T; van Steeg H; de Vries A. 2005. Mice expressing a mammary gland-specific R270H mutation in the p53 tumor suppressor gene mimic human breast cancer development. Cancer Res 65(18):8166-73. [PubMed: 16166291] [MGI Ref ID J:101617]
Wyckoff JB; Wang Y; Lin EY; Li JF; Goswami S; Stanley ER; Segall JE; Pollard JW; Condeelis J. 2007. Direct visualization of macrophage-assisted tumor cell intravasation in mammary tumors. Cancer Res 67(6):2649-56. [PubMed: 17363585] [MGI Ref ID J:120318]
Xu X; Wagner KU; Larson D; Weaver Z; Li C; Ried T; Hennighausen L ; Wynshaw-Boris A ; Deng CX. 1999. Conditional mutation of Brca1 in mammary epithelial cells results in blunted ductal morphogenesis and tumour formation [see comments] Nat Genet 22(1):37-43. [PubMed: 10319859] [MGI Ref ID J:54533]
Zhang D; Hirota T; Marumoto T; Shimizu M; Kunitoku N; Sasayama T; Arima Y; Feng L; Suzuki M; Takeya M; Saya H. 2004. Cre-loxP-controlled periodic Aurora-A overexpression induces mitotic abnormalities and hyperplasia in mammary glands of mouse models. Oncogene 23(54):8720-30. [PubMed: 15480417] [MGI Ref ID J:94713]
Zhao L; Hart S; Cheng J; Melenhorst JJ; Bierie B; Ernst M; Stewart C; Schaper F; Heinrich PC; Ullrich A; Robinson GW; Hennighausen L. 2004. Mammary gland remodeling depends on gp130 signaling through Stat3 and MAPK. J Biol Chem 279(42):44093-100. [PubMed: 15292206] [MGI Ref ID J:93986]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred together.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.