Strain Name:

B6.129P2-Cbfbtm1Itan/J

Stock Number:

008765

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Availability:

Cryopreserved - Ready for recovery

These mice possess loxP sites on either side of exon 5 in the targeted gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

When bred to CD4-cre mice, homozyogous animals have reduced peripheral T cell numbers, abnormal CD4/CD8 expression, and altered response to antigen receptor stimulation. In addition, these mice show autoimmune colitis and asthma-like syndrome.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN8pN1
Generation Definitions
 
Donating InvestigatorDr. Dan R. Littman,   New York University Medical Center

Description
These mice possess loxP sites on either side of exon 5 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

When bred to CD4-cre mice, homozyogous animals have reduced peripheral T cell numbers, abnormal CD4/CD8 expression, and altered response to antigen receptor stimulation. In addition, these mice show autoimmune colitis and asthma-like syndrome.

Development
A floxed neomycin resistance cassette was placed in intron 4 and a loxP site was placed in intron 5. The mutation was introduced using 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Cre expression in the embryonic stem cells excised the floxed neomycin cassette, leaving exon 5 flanked by loxP sites. This line was backcrossed to C57BL/6 for eight generations by the donating laboratory.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cbfb
017411   FVB/N-CbfbTgTn(sb-cHS4,Tyr)2512E-2Ove/Mmjax
View Strains carrying other alleles of Cbfb     (1 strain)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
Immunodeficiency
      T cell deficiency
Inflammation
      Inflammatory bowel disease

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Cbfbtm1Itan
Allele Name targeted mutation 1, Ichiro Taniuchi
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Cbfbetaf; Cbfbetaflox;
Mutation Made By Ichiro Taniuchi,   Inst. of Physical and Chemical Research
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Cbfb, core binding factor beta
Chromosome 8
Gene Common Name(s) AI893578; PEA2; PEBP2B; Pebp2; Pebpb2; expressed sequence AI893578;
Molecular Note A floxed neomycin resistance cassette was placed adjacent to exon 5 and a loxP site was placed in the other flanking intron. The neomycin resistance cassette was subsequently removed by transient expression of Cre recombinase, leaving exon 5 flanked by loxP sites [MGI Ref ID J:125953]

Genotyping

Genotyping Information

Genotyping Protocols

Cbfbtm1ltan, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Cbfbtm1Itan related

Boucheron N; Tschismarov R; Goeschl L; Moser MA; Lagger S; Sakaguchi S; Winter M; Lenz F; Vitko D; Breitwieser FP; Muller L; Hassan H; Bennett KL; Colinge J; Schreiner W; Egawa T; Taniuchi I; Matthias P; Seiser C; Ellmeier W. 2014. CD4(+) T cell lineage integrity is controlled by the histone deacetylases HDAC1 and HDAC2. Nat Immunol 15(5):439-48. [PubMed: 24681565]  [MGI Ref ID J:209977]

Bruno L; Mazzarella L; Hoogenkamp M; Hertweck A; Cobb BS; Sauer S; Hadjur S; Leleu M; Naoe Y; Telfer JC; Bonifer C; Taniuchi I; Fisher AG; Merkenschlager M. 2009. Runx proteins regulate Foxp3 expression. J Exp Med 206(11):2329-37. [PubMed: 19841090]  [MGI Ref ID J:154164]

Chen W; Ma J; Zhu G; Jules J; Wu M; McConnell M; Tian F; Paulson C; Zhou X; Wang L; Li YP. 2014. Cbfbeta deletion in mice recapitulates cleidocranial dysplasia and reveals multiple functions of Cbfbeta required for skeletal development. Proc Natl Acad Sci U S A 111(23):8482-7. [PubMed: 24850862]  [MGI Ref ID J:211343]

Chong MM; Simpson N; Ciofani M; Chen G; Collins A; Littman DR. 2010. Epigenetic propagation of CD4 expression is established by the Cd4 proximal enhancer in helper T cells. Genes Dev 24(7):659-69. [PubMed: 20360383]  [MGI Ref ID J:158962]

Collins A; Hewitt SL; Chaumeil J; Sellars M; Micsinai M; Allinne J; Parisi F; Nora EP; Bolland DJ; Corcoran AE; Kluger Y; Bosselut R; Ellmeier W; Chong MM; Littman DR; Skok JA. 2011. RUNX Transcription Factor-Mediated Association of Cd4 and Cd8 Enables Coordinate Gene Regulation. Immunity 34(3):303-14. [PubMed: 21435585]  [MGI Ref ID J:169841]

Egawa T; Littman DR. 2008. ThPOK acts late in specification of the helper T cell lineage and suppresses Runx-mediated commitment to the cytotoxic T cell lineage. Nat Immunol 9(10):1131-9. [PubMed: 18776905]  [MGI Ref ID J:141144]

Goyama S; Schibler J; Cunningham L; Zhang Y; Rao Y; Nishimoto N; Nakagawa M; Olsson A; Wunderlich M; Link KA; Mizukawa B; Grimes HL; Kurokawa M; Liu PP; Huang G; Mulloy JC. 2013. Transcription factor RUNX1 promotes survival of acute myeloid leukemia cells. J Clin Invest 123(9):3876-88. [PubMed: 23979164]  [MGI Ref ID J:201581]

Kitoh A; Ono M; Naoe Y; Ohkura N; Yamaguchi T; Yaguchi H; Kitabayashi I; Tsukada T; Nomura T; Miyachi Y; Taniuchi I; Sakaguchi S. 2009. Indispensable role of the Runx1-Cbfbeta transcription complex for in vivo-suppressive function of FoxP3+ regulatory T cells. Immunity 31(4):609-20. [PubMed: 19800266]  [MGI Ref ID J:153817]

Klunker S; Chong MM; Mantel PY; Palomares O; Bassin C; Ziegler M; Ruckert B; Meiler F; Akdis M; Littman DR; Akdis CA. 2009. Transcription factors RUNX1 and RUNX3 in the induction and suppressive function of Foxp3+ inducible regulatory T cells. J Exp Med 206(12):2701-15. [PubMed: 19917773]  [MGI Ref ID J:155453]

Kurosaka H; Islam MN; Kuremoto K; Hayano S; Nakamura M; Kawanabe N; Yanagita T; Rice DP; Harada H; Taniuchi I; Yamashiro T. 2011. Core binding factor beta functions in the maintenance of stem cells and orchestrates continuous proliferation and differentiation in mouse incisors. Stem Cells 29(11):1792-803. [PubMed: 21898689]  [MGI Ref ID J:190203]

Naoe Y; Setoguchi R; Akiyama K; Muroi S; Kuroda M; Hatam F; Littman DR; Taniuchi I. 2007. Repression of interleukin-4 in T helper type 1 cells by Runx/Cbf beta binding to the Il4 silencer. J Exp Med 204(8):1749-55. [PubMed: 17646405]  [MGI Ref ID J:125953]

Rudra D; Egawa T; Chong MM; Treuting P; Littman DR; Rudensky AY. 2009. Runx-CBFbeta complexes control expression of the transcription factor Foxp3 in regulatory T cells. Nat Immunol 10(11):1170-7. [PubMed: 19767756]  [MGI Ref ID J:157751]

Satpathy AT; Briseno CG; Cai X; Michael DG; Chou C; Hsiung S; Bhattacharya D; Speck NA; Egawa T. 2014. Runx1 and Cbfbeta regulate the development of Flt3+ dendritic cell progenitors and restrict myeloproliferative disorder. Blood 123(19):2968-77. [PubMed: 24677539]  [MGI Ref ID J:212417]

Seo W; Ikawa T; Kawamoto H; Taniuchi I. 2012. Runx1-Cbfbeta facilitates early B lymphocyte development by regulating expression of Ebf1. J Exp Med 209(7):1255-62. [PubMed: 22665574]  [MGI Ref ID J:189102]

Setoguchi R; Tachibana M; Naoe Y; Muroi S; Akiyama K; Tezuka C; Okuda T; Taniuchi I. 2008. Repression of the transcription factor Th-POK by Runx complexes in cytotoxic T cell development. Science 319(5864):822-5. [PubMed: 18258917]  [MGI Ref ID J:131081]

Tober J; Yzaguirre AD; Piwarzyk E; Speck NA. 2013. Distinct temporal requirements for Runx1 in hematopoietic progenitors and stem cells. Development 140(18):3765-76. [PubMed: 23924635]  [MGI Ref ID J:204463]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, heterozygous or homozygous mice may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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