Description

Strain Information

Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   29-MAR-10 Species laboratory mouse Generation N8+F5 (06-OCT-11) Generation Definitions   Donating Investigator Dan Littman,   New York University Medical Center

Description
These mice possess loxP sites on either side of exon 5 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

When bred to CD4-cre mice, homozyogous animals have reduced peripheral T cell numbers, abnormal CD4/CD8 expression, and altered response to antigen receptor stimulation. In addition, these mice show autoimmune colitis and asthma-like syndrome.

Development
A floxed neomycin resistance cassette was placed in intron 4 and a loxP site was placed in intron 5. The mutation was introduced using 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Cre expression in the embryonic stem cells excised the floxed neomycin cassette, leaving exon 5 flanked by loxP sites. This line was backcrossed to C57BL/6 for eight generations by the donating laboratory.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
Autoimmunity
Immunodeficiency
      T cell deficiency
Inflammation
      Inflammatory bowel disease

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Cbfbtm1Itan
Allele Name		targeted mutation 1, Ichiro Taniuchi
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		Cbfbetaf; Cbfbetaflox;
Mutation Made By		Ichiro Taniuchi,   Inst. of Physical and Chemical Research
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14
ES Cell Line Strain		129P2/OlaHsd
Gene Symbol and Name		Cbfb, core binding factor beta
Chromosome		8
Gene Common Name(s)		AI893578; PEA2; PEBP2B; Pebp2; Pebpb2; expressed sequence AI893578;
Molecular Note		A floxed neomycin resistance cassette was placed adjacent to exon 5 and a loxP site was placed in the other flanking intron. The neomycin resistance cassette was subsequently removed by transient expression of Cre recombinase, leaving exon 5 flanked by loxP sites [MGI Ref ID J:125953]

Genotyping

Genotyping Information

Genotyping Protocols

Cbfb^tm1ltan, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Naoe Y; Setoguchi R; Akiyama K; Muroi S; Kuroda M; Hatam F; Littman DR; Taniuchi I. 2007. Repression of interleukin-4 in T helper type 1 cells by Runx/Cbf beta binding to the Il4 silencer. J Exp Med 204(8):1749-55. [PubMed: 17646405]  [MGI Ref ID J:125953]

Additional References

Cbfb^tm1Itan related

Bruno L; Mazzarella L; Hoogenkamp M; Hertweck A; Cobb BS; Sauer S; Hadjur S; Leleu M; Naoe Y; Telfer JC; Bonifer C; Taniuchi I; Fisher AG; Merkenschlager M. 2009. Runx proteins regulate Foxp3 expression. J Exp Med 206(11):2329-37. [PubMed: 19841090]  [MGI Ref ID J:154164]

Chong MM; Simpson N; Ciofani M; Chen G; Collins A; Littman DR. 2010. Epigenetic propagation of CD4 expression is established by the Cd4 proximal enhancer in helper T cells. Genes Dev 24(7):659-69. [PubMed: 20360383]  [MGI Ref ID J:158962]

Collins A; Hewitt SL; Chaumeil J; Sellars M; Micsinai M; Allinne J; Parisi F; Nora EP; Bolland DJ; Corcoran AE; Kluger Y; Bosselut R; Ellmeier W; Chong MM; Littman DR; Skok JA. 2011. RUNX Transcription Factor-Mediated Association of Cd4 and Cd8 Enables Coordinate Gene Regulation. Immunity 34(3):303-14. [PubMed: 21435585]  [MGI Ref ID J:169841]

Egawa T; Littman DR. 2008. ThPOK acts late in specification of the helper T cell lineage and suppresses Runx-mediated commitment to the cytotoxic T cell lineage. Nat Immunol 9(10):1131-9. [PubMed: 18776905]  [MGI Ref ID J:141144]

Kitoh A; Ono M; Naoe Y; Ohkura N; Yamaguchi T; Yaguchi H; Kitabayashi I; Tsukada T; Nomura T; Miyachi Y; Taniuchi I; Sakaguchi S. 2009. Indispensable role of the Runx1-Cbfbeta transcription complex for in vivo-suppressive function of FoxP3+ regulatory T cells. Immunity 31(4):609-20. [PubMed: 19800266]  [MGI Ref ID J:153817]

Klunker S; Chong MM; Mantel PY; Palomares O; Bassin C; Ziegler M; Ruckert B; Meiler F; Akdis M; Littman DR; Akdis CA. 2009. Transcription factors RUNX1 and RUNX3 in the induction and suppressive function of Foxp3+ inducible regulatory T cells. J Exp Med 206(12):2701-15. [PubMed: 19917773]  [MGI Ref ID J:155453]

Rudra D; Egawa T; Chong MM; Treuting P; Littman DR; Rudensky AY. 2009. Runx-CBFbeta complexes control expression of the transcription factor Foxp3 in regulatory T cells. Nat Immunol 10(11):1170-7. [PubMed: 19767756]  [MGI Ref ID J:157751]

Setoguchi R; Tachibana M; Naoe Y; Muroi S; Akiyama K; Tezuka C; Okuda T; Taniuchi I. 2008. Repression of the transcription factor Th-POK by Runx complexes in cytotoxic T cell development. Science 319(5864):822-5. [PubMed: 18258917]  [MGI Ref ID J:131081]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           MGL375

Colony Maintenance

Breeding & Husbandry	When maintained as a live colony, heterozygous or homozygous mice may be bred.
Mating System	Homozygote x Homozygote         (Female x Male)   29-MAR-10
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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