Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N14+N1F3pN1 Generation Definitions   Donating Investigator Dr. Dan R. Littman,   New York University Medical Center

Description
Exon 4 of these targeted mutant mice is flanked by loxP sites. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. When crossed to a CD4-cre mouse strain, progeny have fewer CD4⁺ T cells than wildtype mice and completely lack NK T cells.

Development
A loxP site was placed 5' of exon 4 and a loxP-flanked neomycin cassette was placed in intron 4 of the targeted gene. Transient infection with Cre excised the neomycin cassette, leaving a loxP-flanked exon 4. This mutation was created in 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. This strain was backcrossed 13 times to C57BL/6 (see SNP note below) by the donating laboratory.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Runx1

005669	B6.129S-Runx1tm1Spe/J
010673	B6;129-Runx1tm3.1Spe/J

View Strains carrying other alleles of Runx1     (2 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Leukemia, Acute Myeloid; AML   (RUNX1) Platelet Disorder, Familial, with Associated Myeloid Malignancy   (RUNX1)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Immunology and Inflammation Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Runx1tm1Tani
Allele Name		targeted mutation 1, Ichiro Taniuchi
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		Runx1F;
Mutation Made By		Ichiro Taniuchi,   Inst. of Physical and Chemical Research
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14
ES Cell Line Strain		129P2/OlaHsd
Gene Symbol and Name		Runx1, runt related transcription factor 1
Chromosome		16
Gene Common Name(s)		AI462102; AML1; AML1-EVI-1; AMLCR1; CBFA2; Cbfa2; EVI-1; PEBP2aB; Pebp2a2; core binding factor alpha 2; expressed sequence AI462102; runt domain, alpha subunit 2;
Molecular Note		Exon 4 was floxed by insertion of a single upstream loxP site and a downstream floxed neo cassette. The neo cassette was subsequently removed by transient expression of cre recombinase in correctly targeted ES cells, leaving exon 4 flanked by loxP sites. [MGI Ref ID J:80690]

Genotyping

Genotyping Information

Genotyping Protocols

Runx1^tm1Tani, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Taniuchi I; Osato M; Egawa T; Sunshine MJ; Bae SC; Komori T; Ito Y; Littman DR. 2002. Differential Requirements for Runx Proteins in CD4 Repression and Epigenetic Silencing during T Lymphocyte Development. Cell 111(5):621-33. [PubMed: 12464175]  [MGI Ref ID J:80690]

Additional References

Runx1^tm1Tani related

Egawa T; Eberl G; Taniuchi I; Benlagha K; Geissmann F; Hennighausen L; Bendelac A; Littman DR. 2005. Genetic evidence supporting selection of the valpha14i NKT cell lineage from double-positive thymocyte precursors. Immunity 22(6):705-16. [PubMed: 15963785]  [MGI Ref ID J:99111]

Egawa T; Littman DR. 2008. ThPOK acts late in specification of the helper T cell lineage and suppresses Runx-mediated commitment to the cytotoxic T cell lineage. Nat Immunol 9(10):1131-9. [PubMed: 18776905]  [MGI Ref ID J:141144]

Egawa T; Tillman RE; Naoe Y; Taniuchi I; Littman DR. 2007. The role of the Runx transcription factors in thymocyte differentiation and in homeostasis of naive T cells. J Exp Med 204(8):1945-57. [PubMed: 17646406]  [MGI Ref ID J:125959]

Kitoh A; Ono M; Naoe Y; Ohkura N; Yamaguchi T; Yaguchi H; Kitabayashi I; Tsukada T; Nomura T; Miyachi Y; Taniuchi I; Sakaguchi S. 2009. Indispensable role of the Runx1-Cbfbeta transcription complex for in vivo-suppressive function of FoxP3+ regulatory T cells. Immunity 31(4):609-20. [PubMed: 19800266]  [MGI Ref ID J:153817]

Lukin K; Fields S; Guerrettaz L; Straign D; Rodriguez V; Zandi S; Mansson R; Cambier JC; Sigvardsson M; Hagman J. 2011. A dose-dependent role for EBF1 in repressing non-B-cell-specific genes. Eur J Immunol 41(6):1787-93. [PubMed: 21469119]  [MGI Ref ID J:176486]

Lukin K; Fields S; Lopez D; Cherrier M; Ternyak K; Ramirez J; Feeney AJ; Hagman J. 2010. Compound haploinsufficiencies of Ebf1 and Runx1 genes impede B cell lineage progression. Proc Natl Acad Sci U S A 107(17):7869-74. [PubMed: 20385820]  [MGI Ref ID J:159374]

Rudra D; Egawa T; Chong MM; Treuting P; Littman DR; Rudensky AY. 2009. Runx-CBFbeta complexes control expression of the transcription factor Foxp3 in regulatory T cells. Nat Immunol 10(11):1170-7. [PubMed: 19767756]  [MGI Ref ID J:157751]

Seo W; Ikawa T; Kawamoto H; Taniuchi I. 2012. Runx1-Cbfbeta facilitates early B lymphocyte development by regulating expression of Ebf1. J Exp Med 209(7):1255-62. [PubMed: 22665574]  [MGI Ref ID J:189102]

Setoguchi R; Tachibana M; Naoe Y; Muroi S; Akiyama K; Tezuka C; Okuda T; Taniuchi I. 2008. Repression of the transcription factor Th-POK by Runx complexes in cytotoxic T cell development. Science 319(5864):822-5. [PubMed: 18258917]  [MGI Ref ID J:131081]

Wang X; Blagden C; Fan J; Nowak SJ; Taniuchi I; Littman DR; Burden SJ. 2005. Runx1 prevents wasting, myofibrillar disorganization, and autophagy of skeletal muscle. Genes Dev 19(14):1715-22. [PubMed: 16024660]  [MGI Ref ID J:99622]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintained as a live colony, homozygotes may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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