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| Exon 4 of these targeted mutant mice is flanked by loxP sites. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. When crossed to either a CD4-cre or Lck-cre mouse strain, the numbers of CD8+ mature thymocytes and CD8+ T cells in spleen or lymph nodes are reduced and show defective responses to antigen receptor stimulation. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Dr. Dan R. Littman, New York University Medical Center Description
Exon 4 of these targeted mutant mice is flanked by loxP sites. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.When crossed to either a CD4-cre or Lck-cre mouse strain, the numbers of CD8+ mature thymocytes and CD8+ T cells in spleen or lymph nodes are reduced and show defective responses to antigen receptor stimulation. CD8+ T cells express CD4 and the ectopic CD4 expression is enhanced when the floxed region is excised.
Development
A loxP-flanked neomycin cassette was placed in intron 3, and an additional loxP site was introduced to intron 4 of the targeted gene. Transient infection with Cre excised the neomycin cassette, leaving exon 4 flanked by loxP sites. This mutation was created in 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. This strain was backcrossed ten times to C57BL/6 (see SNP note below) by the donating laboratory.A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Runx3
008774 B6;129P2-Runx3tm1Litt/J View Strains carrying other alleles of Runx3 (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System
loxP-flanked Sequences
Immunology and Inflammation Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Runx3tm1Itan | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Ichior Taniuchi | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | Runx3f; Runx3flox; | ||
| Mutation Made By | Ichiro Taniuchi, Inst. of Physical and Chemical Research | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Runx3, runt related transcription factor 3 | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | AML2; CBFA3; Cbfa3; PEBP2aC; core binding factor alpha 3; | ||
| Molecular Note | A floxed neomycin selection cassette was placed in an adjacent intron of a coding exon and was subsequently removed, leaving behind a single loxP site. A second loxP site was introduced in a downstream intron to "flox" the coding exon. [MGI Ref ID J:125953] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Runx3tm1Itan, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Naoe Y; Setoguchi R; Akiyama K; Muroi S; Kuroda M; Hatam F; Littman DR; Taniuchi I. 2007. Repression of interleukin-4 in T helper type 1 cells by Runx/Cbf beta binding to the Il4 silencer. J Exp Med 204(8):1749-55. [PubMed: 17646405] [MGI Ref ID J:125953]
Additional References
Runx3tm1Itan relatedEgawa T; Littman DR. 2008. ThPOK acts late in specification of the helper T cell lineage and suppresses Runx-mediated commitment to the cytotoxic T cell lineage. Nat Immunol 9(10):1131-9. [PubMed: 18776905] [MGI Ref ID J:141144]
Egawa T; Tillman RE; Naoe Y; Taniuchi I; Littman DR. 2007. The role of the Runx transcription factors in thymocyte differentiation and in homeostasis of naive T cells. J Exp Med 204(8):1945-57. [PubMed: 17646406] [MGI Ref ID J:125959]
Kitoh A; Ono M; Naoe Y; Ohkura N; Yamaguchi T; Yaguchi H; Kitabayashi I; Tsukada T; Nomura T; Miyachi Y; Taniuchi I; Sakaguchi S. 2009. Indispensable role of the Runx1-Cbfbeta transcription complex for in vivo-suppressive function of FoxP3+ regulatory T cells. Immunity 31(4):609-20. [PubMed: 19800266] [MGI Ref ID J:153817]
Seo W; Ikawa T; Kawamoto H; Taniuchi I. 2012. Runx1-Cbfbeta facilitates early B lymphocyte development by regulating expression of Ebf1. J Exp Med 209(7):1255-62. [PubMed: 22665574] [MGI Ref ID J:189102]
Setoguchi R; Tachibana M; Naoe Y; Muroi S; Akiyama K; Tezuka C; Okuda T; Taniuchi I. 2008. Repression of the transcription factor Th-POK by Runx complexes in cytotoxic T cell development. Science 319(5864):822-5. [PubMed: 18258917] [MGI Ref ID J:131081]
Xiong Y; Castro E; Yagi R; Zhu J; Lesourne R; Love PE; Feigenbaum L; Bosselut R. 2013. Thpok-independent repression of Runx3 by Gata3 during CD4(+) T-cell differentiation in the thymus. Eur J Immunol 43(4):918-28. [PubMed: 23310955] [MGI Ref ID J:195075]
Yagi R; Junttila IS; Wei G; Urban JF Jr; Zhao K; Paul WE; Zhu J. 2010. The transcription factor GATA3 actively represses RUNX3 protein-regulated production of interferon-gamma. Immunity 32(4):507-17. [PubMed: 20399120] [MGI Ref ID J:160369]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintained as a live colony, either heterozygotes or homozygotes may be bred.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2085.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2710.50 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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