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| These transgenic mice express Cre recombinase under the control of the mouse kidney androgen regulated protein (Kap). Cre recombinase expression is detected in the proximal tubule cells of the renal cortex in male mice. Female mice do not express the transgene unless treated with androgen (testosterone). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the proximal tubule cells of the kidney. | |||||||||||
- Description
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Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 28-APR-09 Species laboratory mouse Donating Investigator Curt Sigmund, University of Iowa College of Medicine Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase (iCre, improved cre) under the control of the mouse kidney androgen regulated protein (Kap). Cre recombinase expression is detected in the proximal tubule cells of the renal cortex in male mice. Female mice do not express the transgene unless treated with androgen (testosterone). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the proximal tubule cells of the kidney.Development
A transgenic construct containing cre coding sequence (iCre, improved cre) under the control of the mouse kidney androgen regulated protein (Kap) was injected into fertilized C57BL/6J X SJL/J mouse eggs. The resulting founder mice were backcrossed for 6 generations on the C57BL/6J background.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (162 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Tissue/Cell Markers: kidney specific marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Kap-cre)29066/2Sig | ||
|---|---|---|---|
| Allele Name | transgene insertion 29066/2, Curt Sigmund | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | KAP2-iCre; | ||
| Mutation Made By | Curt Sigmund, University of Iowa College of Medicine | ||
| Strain of Origin | C57BL/6J x SJL | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Kap, kidney androgen regulated protein, mouse, laboratory | ||
| Driver Note | Kap | ||
| Molecular Note | The Kap promoter is used to drive androgen inducible improved cre (crei) in the kidney. Twent-six founders were created of which only line 29066/2 expressed cre in male kidneys with some expression in the brain, liver and heart. [MGI Ref ID J:137742] | ||
| Gene Symbol and Name | Tg(Kap-cre)29066/2Sig, transgene insertion 29066/2, Curt Sigmund | ||
| Chromosome | UN | ||
| Gene Common Name(s) | KAP2-iCre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic iCre, Melt Curve Analysis
Generic iCre, Standard PCR
Generic iCre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Li H; Zhou X; Davis DR; Xu D; Sigmund CD. 2008. An androgen-inducible proximal tubule-specific Cre recombinase transgenic model. Am J Physiol Renal Physiol 294(6):F1481-6. [PubMed: 18385272] [MGI Ref ID J:137742]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375/MGL377
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator has not attempted to make the strain homozygous. Mating System Noncarrier x Hemizygote (Female x Male) 28-APR-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Kap-cre)29066/2Sig
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Kap-cre)29066/2Sig x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Kap-cre)29066/2Sig
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Kap-cre)29066/2Sig
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Kap-cre)29066/2Sig x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Kap-cre)29066/2Sig
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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