|These transgenic mice express a myc peptide tagged mutant (truncated) human DISC1 gene under the control of tetracycline operator (tetO). When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) to create a bitransgenic animal, tissue-specific transgene expression can be regulated with the tetracycline analog, doxycycline. When bred to a strain expressing rtTA or tTA in brain tissues (see Stock No. 003010, B6;CBA-Tg(Camk2a-tTA)1Mmay/J, for example), this mutant mouse strain may be useful in studies of psychiatric disorders such as schizophrenia.|
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Mikhail Pletnikov, Johns Hopkins University
These transgenic mice express a myc peptide-tagged, mutant (truncated) human DISC1 (disrupted in schizophrenia 1) gene under the control of tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator). The Donating Investigator reports that mice homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) to create a bitransgenic animal, tissue-specific mutant DISC1 transgene expression can be regulated with the tetracycline analog, doxycycline. When bred to a strain expressing rtTA or tTA in brain tissues (see Stock No. 003010, B6;CBA-Tg(Camk2a-tTA)1Mmay/J, for example), this mutant mouse strain may be useful in studies of psychiatric disorders such as schizophrenia.
The Tg(TRE-CMVhDISC1) transgene was designed with the myc peptide tagged mutant human DISC1 (disrupted in schizophrenia 1) gene, under the control of tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) sequence and a cytomegalovirus minimal promoter. This transgene was injected into fertilized B6;SJL mouse eggs. Founder line 1001 was subsequently established. The mice were then crossed to CBA and possibly C3H.
|Considerations for Choosing Controls|
Strains carrying other alleles of tetO
008079 129S-Ppargtm2Yba/J 016178 B6(Cg)-Tg(tetO-Cry2)3Jt/J 016176 B6(Cg)-Tg(tetO-Per2)2Jt/J 023757 B6(Cg)-Tg(tetO-tetX,lacZ)1Gogo/UmriJ 009602 B6.129S4(Cg)-Kcnn2tm2Jpad/J 009603 B6.129S4-Kcnn3tm1Jpad/J 023910 B6.Cg-Col1a1tm1(tetO-Lin28a)Gqda/J 023911 B6.Cg-Col1a1tm2(tetO-LIN28B)Gqda/J 023912 B6.Cg-Col1a1tm3(tetO-Mirlet7g/Mir21)Gqda/J 017983 B6.Cg-Col1a1tm9(tetO-Dnmt3b_i1)Jae Gt(ROSA)26Sortm1(rtTA*M2)Jae/J 014588 B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm6(tetO-MSI2)Jae/J 023749 B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Tg(tetO-Pou5f1,-Sox2,-Klf4,-Myc)1Srn/J 014648 B6.Cg-Gt(ROSA)26Sortm37(H1/tetO-RNAi:Taz)Arte/ZkhuJ 006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J 016998 B6.Cg-Tg(TetO-Axin1,EGFP)TA6Cos/J 003762 B6.Cg-Tg(tetFosb)4468Nes/J 007051 B6.Cg-Tg(tetO-APPSwInd)102Dbo/Mmjax 007052 B6.Cg-Tg(tetO-APPSwInd)107Dbo/Mmjax 007049 B6.Cg-Tg(tetO-APPSwInd)885Dbo/Mmjax 007618 B6.Cg-Tg(tetO-Arntl)1Jt/J 017555 B6.Cg-Tg(tetO-CALY)5Cber/J 024114 B6.Cg-Tg(tetO-CHRM4*)2Blr/J 008277 B6.Cg-Tg(tetO-Clockm1Jt)CL57Jt/J 008468 B6.Cg-Tg(tetO-DTA)1Gfi/J 017791 B6.Cg-Tg(tetO-Hamp)2181Nca/J 009344 B6.Cg-Tg(tetO-Ifng)184Pop/J 009136 B6.Cg-Tg(tetO-Kcnj2,lacZ)1Gogo/J 013583 B6.Cg-Tg(tetO-LRRK2)C7874Cai/J 020652 B6.Cg-Tg(tetO-Mif)279Aren/J 017331 B6.Cg-Tg(tetO-Ppp3ca*)11255Kndl/J 017332 B6.Cg-Tg(tetO-Ppp3ca*)13967Kndl/J 017330 B6.Cg-Tg(tetO-TAg*)175Kndl/J 006234 B6.Cg-Tg(tetO-cre)1Jaw/J 005738 B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J 021025 B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-cre)Haho/J 006911 B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J 011001 B6;129S4-Col1a1tm1(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Hoch/J 016836 B6;129S4-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm7(tetO-HIST1H2BJ/GFP)Jae/J 012433 B6;C3-Tg(ACTA1-rtTA,tetO-cre)102Monk/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 023598 B6;C3-Tg(tetO-AIMP2)630Tmd/J 023642 B6;C3-Tg(tetO-AIMP2)634Tmd/J 016841 B6;C3-Tg(tetO-TARDBP)12Vle/J 014650 B6;C3-Tg(tetO-TARDBP*)4Vle/J 012450 B6;D2-Tg(tetO-SNCA)1Cai/J 008344 B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J 024742 B6;DBA-Tg(tetO-GCaMP6s)2Niell/J 024088 B6;FVB-Tg(tetO-AML1/ETO)8Dzh/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 010575 B6;SJL-Tg(tetO-Egfr*)2-9Jek/J 010577 B6;SJL-Tg(tetO-Erbb2*)8-4Jek/J 002621 B6;SJL-Tg(tetop-lacZ)2Mam/J 006004 B6C3-Tg(tetO-APPSwInd)885Dbo/Mmjax 016976 B6C3-Tg(tetO-SNCA*A53T)33Vle/J 018913 B6N.Cg-Tg(tetO-GFP,-lacZ)G3Rsp/J 006244 C.Cg-Tg(tetO-cre)1Jaw/J 017719 C3HeB/FeJ-Tg(tetO-TAg)1Efr/J 017955 C57BL/6-Tg(Gfap-rtTA,tetO-MAOB,-lacZ)1Jkan/J 005706 C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 017613 C57BL/6-Tg(tetO-Cdkn1b)1Scpr/J 013729 C57BL/6-Tg(tetO-EDN1,-lacZ)9Mhus/J 016260 C57BL/6-Tg(tetO-Fbxl21)38Jt/J 016179 C57BL/6-Tg(tetO-Fbxl21*)11Jt/J 010713 C57BL/6-Tg(tetO-GFP/tetX)5696Stl/J 013728 C57BL/6-Tg(tetO-NOS2,-lacZ)240iMhus/J 016181 C57BL/6-Tg(tetO-Nr1d1)1Schb/J 016581 C57BL/6J-Tg(tetO-Btrc*)1Jt/J 008278 C57BL/6J-Tg(tetO-Clock)1Jt/J 024898 C57BL/6J-Tg(tetO-EGFP/Rpl10a)5aReij/J 016580 C57BL/6J-Tg(tetO-Usf1)2Jt/J 021065 FVB(C)-Tg(tetO-Npc1/YFP)1Mps/J 017542 FVB-Tg(Myh6/tetO-ATP2B4)1Jmol/J 016571 FVB-Tg(Myh6/tetO-Gata6)2Jmol/J 014155 FVB-Tg(Myh6/tetO-Itpr1)22.3Jmol/J 014153 FVB-Tg(Myh6/tetO-Itpr2)3.11Jmol/J 014154 FVB-Tg(Myh6/tetO-Itpr2)4.9Jmol/J 012684 FVB-Tg(Myh6/tetO-POSTN)22.1Jmol/J 010580 FVB-Tg(Myh6/tetO-PRKCA*)1Jmk/J 013156 FVB-Tg(tetO-CDK5R1*)1Vln/J 013777 FVB-Tg(tetO-Cacna1g)1Jmol/J 013778 FVB-Tg(tetO-Cacnb2)1Jmol/J 013779 FVB-Tg(tetO-Cacnb2)2Jmol/J 013780 FVB-Tg(tetO-Cib1)1Jmol/J 010578 FVB-Tg(tetO-Dusp6)1Jmol/J 017333 FVB-Tg(tetO-Gnai2*,-lacZ)382Kndl/J 008685 FVB-Tg(tetO-Kdr*)4377.5Rwng/J 023397 FVB-Tg(tetO-Lmnb1)AF1Yfu/J 015815 FVB-Tg(tetO-MAPT*P301L)#Kha/JlwsJ 008695 FVB-Tg(tetO-MET)23Rwng/J 012387 FVB-Tg(tetO-Ppargc1a)1Dpk/J 012385 FVB-Tg(tetO-Ppargc1b)7Dpk/J 022979 FVB-Tg(tetO-Thbs4)17.7Jmol/J 006439 FVB-Tg(tetO/CMV-KRAS*G12C)9.1Msmi/J 019038 FVB.Cg-Tg(tetO-GLI1)10Rup/Mmjax 019039 FVB.Cg-Tg(tetO-KLF4)32831Rup/Mmjax 008244 FVB.Cg-Tg(tetO-cre)1Jaw/J 012459 FVB/N-Tg(Myh6*/tetO-Capn1)L2Gwd/J 005941 FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J 006202 FVB/N-Tg(tetO-BCR/ABL1)2Dgt/J 014547 FVB/N-Tg(tetO-Fasl)BDepa/J 025672 FVB/N-Tg(tetO-Fgfr2b/Igh)1.3Jaw/J 019376 FVB/N-Tg(tetO-MYC)36aBop/J 022938 FVB/N-Tg(tetO-Wnt5a)17Rva/J 003315 FVB/N-Tg(tetORo1-lacZ)3Conk/J 005076 NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ 006999 STOCK Dbttm1Geh Tg(Cebpb-tTA)5Bjd Tg(tetO-DBT)A1Geh/J 011004 STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J 011011 STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J 011013 STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm5(tetO-Pou5f1,-Klf4,-Myc)Jae/J 018999 STOCK Gt(ROSA)26Sortm1(tTA,tetO-Mir155)Fjsl/J 017596 STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#aAhmb/J 017597 STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#bAhmb/J 025671 STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(tetO-Fgf10)1Jaw/SpdlJ 024854 STOCK Tg(Camk2a-tTA)1Mmay Tg(tetO-MAPT*P301L)#Kha/J 008755 STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J 012477 STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J 016572 STOCK Tg(Myh6/tetO-Gata4)1Jmol/J 014544 STOCK Tg(tetO-ABL1*P242E*P249E)CPdav/J 014093 STOCK Tg(tetO-CHRM3*)1Blr/J 008168 STOCK Tg(tetO-DTA)1Gfi/J 017755 STOCK Tg(tetO-GCAMP2)12iRyu/J 024509 STOCK Tg(tetO-Gata6)1Abl/J 016970 STOCK Tg(tetO-HCV)1Mlch/Mmjax 005104 STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J 005699 STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J 005728 STOCK Tg(tetO-Ipf1,lacZ)958.1Macd/J 012441 STOCK Tg(tetO-LRRK2*G2019S)E3Cai/J 017918 STOCK Tg(tetO-MAML1*/EGFP)2Akar/J 017599 STOCK Tg(tetO-SMN2,-luc)#aAhmb/J 017600 STOCK Tg(tetO-SMN2,-luc)#bAhmb/J 012442 STOCK Tg(tetO-SNCA*A53T)E2Cai/J 006224 STOCK Tg(tetO-cre)1Jaw/J 017906 STOCK Tg(tetO-hop/EGFP,-COP4/mCherry)6Kftnk/J 012345 STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J 012449 STOCK Tg(teto-LRRK2)C7874Cai/JView Strains carrying other alleles of tetO (136 strains)
View Related Disease (OMIM) TermsRelated Disease (OMIM) Terms provided by MGIView Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services firstname.lastname@example.org for customized breeding options.
Tg(Camk2a-tTA)1Mmay/0 Tg(tetO-DISC1*)1001Plet/0involves: C57BL/6 * CBA * SJL
- behavior/neurological phenotype
- abnormal social/conspecific interaction
- male mutants spend significantly less time in non-aggressive social interaction with other males (MGI Ref ID J:146881)
- increased aggression towards mice
- males display increased aggressiveness toward other males, with an increase in number of attacks (MGI Ref ID J:146881)
- abnormal spatial reference memory
- spatial memory in females is impaired in probe trials, with females spending less time in the target quadrant where the platform had previously been (MGI Ref ID J:146881)
- male mutants show higher degree of spontaneous locomotor activity than wild-type littermate controls during a 20-hour period (MGI Ref ID J:146881)
- nervous system phenotype
- abnormal lateral ventricle morphology
- volume is significantly increased, with no change in total brain volume (MGI Ref ID J:146881)
- abnormal neuron morphology
- cultured control neurons grow more complex neurite arbors than mutant neurons (for 20-100 um rings centered on neuron soma) (MGI Ref ID J:146881)View Research Applications
|Allele Name||transgene insertion 1001, Mikhail Pletnikov|
|Allele Type||Transgenic (Inducible, Inserted expressed sequence)|
|Common Name(s)||Tg(TRE-CMVhDISC1); Tg(tetO/CMV-DISC1*)1001Plet;|
|Strain of Origin||C57BL/6 and SJL|
|Expressed Gene||DISC1, disrupted in schizophrenia 1, human|
|Promoter||tetO, tet operator,|
|Molecular Note||The transgene construct was designed with the myc peptide tagged mutant human DISC1 (disrupted in schizophrenia 1) gene, under the control of tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) sequence and a cytomegalovirus minimal promoter. Founder line 1001, which showed strong expression, was established. A second line, line 70, shows weak expression of human DISC1. [MGI Ref ID J:146881]|
Tg(teto-DISC1*)1001Plet, Fast MCA
Tg(teto-DISC1*)1001Plet, Standard PCR
Kvajo M; McKellar H; Gogos JA. 2012. Avoiding mouse traps in schizophrenia genetics: lessons and promises from current and emerging mouse models. Neuroscience 211:136-64. [PubMed: 21821099] [MGI Ref ID J:184660]
Pletnikov MV; Ayhan Y; Nikolskaia O; Xu Y; Ovanesov MV; Huang H; Mori S; Moran TH; Ross CA. 2008. Inducible expression of mutant human DISC1 in mice is associated with brain and behavioral abnormalities reminiscent of schizophrenia. Mol Psychiatry 13(2):173-86, 115. [PubMed: 17848917] [MGI Ref ID J:146881]
Pogorelov VM; Nomura J; Kim J; Kannan G; Ayhan Y; Yang C; Taniguchi Y; Abazyan B; Valentine H; Krasnova IN; Kamiya A; Cadet JL; Wong DF; Pletnikov MV. 2012. Mutant DISC1 affects methamphetamine-induced sensitization and conditioned place preference: a comorbidity model. Neuropharmacology 62(3):1242-51. [PubMed: 21315744] [MGI Ref ID J:183592]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes or homozygotes. The Donating Investigator reports that homozygous mice are viable and fertile.
|Pricing for USA, Canada and Mexico shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2140.00
At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
|Pricing for International shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2782.00
Cryorecovery - Standard.
Progeny testing is not required.
|Considerations for Choosing Controls|
|Control Pricing Information for Genetically Engineered Mutant Strains.|
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