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| These transgenic mice express a myc peptide tagged mutant (truncated) human DISC1 gene under the control of tetracycline operator (tetO). When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) to create a bitransgenic animal, tissue-specific transgene expression can be regulated with the tetracycline analog, doxycycline. When bred to a strain expressing rtTA or tTA in brain tissues (see Stock No. 003010, B6;CBA-Tg(Camk2a-tTA)1Mmay/J, for example), this mutant mouse strain may be useful in studies of psychiatric disorders such as schizophrenia. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
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Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System +/+ sibling x Hemizygote (Female x Male) 08-AUG-09 Species laboratory mouse Generation F?+N1 (18-AUG-09) Donating Investigator Mikhail Pletnikov, Johns Hopkins University Description
These transgenic mice express a myc peptide-tagged, mutant (truncated) human DISC1 (disrupted in schizophrenia 1) gene under the control of tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator). The Donating Investigator reports that mice homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) to create a bitransgenic animal, tissue-specific mutant DISC1 transgene expression can be regulated with the tetracycline analog, doxycycline. When bred to a strain expressing rtTA or tTA in brain tissues (see Stock No. 003010, B6;CBA-Tg(Camk2a-tTA)1Mmay/J, for example), this mutant mouse strain may be useful in studies of psychiatric disorders such as schizophrenia.Development
The Tg(TRE-CMVhDISC1) transgene was designed with the myc peptide tagged mutant human DISC1 (disrupted in schizophrenia 1) gene, under the control of tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) sequence and a cytomegalovirus minimal promoter. This transgene was injected into fertilized B6;SJL mouse eggs. Founder line 1001 was subsequently established. The mice were then crossed to CBA and possibly C3H.
Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of tetO
View Strains carrying other alleles of tetO (36 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Tg(Camk2a-tTA)1Mmay/0 Tg(tetO-DISC1*)1001Plet/0
involves: C57BL/6 * CBA * SJL
- behavior/neurological phenotype
- abnormal social/conspecific interaction (MGI Ref ID J:146881)
- male mutants spend significantly less time in non-aggressive social interaction with other males
- increased aggression towards mice (MGI Ref ID J:146881)
- males display increased aggressiveness toward other males, with an increase in number of attacks
- abnormal spatial reference memory (MGI Ref ID J:146881)
- spatial memory in females is impaired in probe trials, with females spending less time in the target quadrant where the platform had previously been
- hyperactivity (MGI Ref ID J:146881)
- male mutants show higher degree of spontaneous locomotor activity than wild-type littermate controls during a 20-hour period
- nervous system phenotype
- abnormal lateral ventricle morphology (MGI Ref ID J:146881)
- volume is significantly increased, with no change in total brain volume
- abnormal neuron morphology (MGI Ref ID J:146881)
- cultured control neurons grow more complex neurite arbors than mutant neurons (for 20-100 um rings centered on neuron soma)
This mouse can be used to support research in many areas including:
Neurobiology Research
Behavioral and Learning Defects
Tet Expression System
tTA/rtTA Responsive Strains
Research Tools
Neurobiology Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Responsive Strains
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(tetO-DISC1*)1001Plet | ||
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| Allele Name | transgene insertion 1001, Mikhail Pletnikov | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Tg(TRE-CMVhDISC1); Tg(tetO/CMV-DISC1*)1001Plet; | ||
| Strain of Origin | C57BL/6 and SJL | ||
| Expressed Gene | DISC1, disrupted in schizophrenia 1, human | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | The transgene construct was designed with the myc peptide tagged mutant human DISC1 (disrupted in schizophrenia 1) gene, under the control of tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) sequence and a cytomegalovirus minimal promoter. Founder line 1001, which showed strong expression, was established. A second line, line 70, shows weak expression of human DISC1. [MGI Ref ID J:146881] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Tg(tetO-DISC1*)1001Plet relatedPletnikov MV; Ayhan Y; Nikolskaia O; Xu Y; Ovanesov MV; Huang H; Mori S; Moran TH; Ross CA. 2008. Inducible expression of mutant human DISC1 in mice is associated with brain and behavioral abnormalities reminiscent of schizophrenia. Mol Psychiatry 13(2):173-86, 115. [PubMed: 17848917] [MGI Ref ID J:146881]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes or homozygotes. The Donating Investigator reports that homozygous mice are viable and fertile. Mating System +/+ sibling x Hemizygote (Female x Male) 08-AUG-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(tetO-DISC1*)1001Plet
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(tetO-DISC1*)1001Plet x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(tetO-DISC1*)1001Plet
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(tetO-DISC1*)1001Plet
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(tetO-DISC1*)1001Plet x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(tetO-DISC1*)1001Plet
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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