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| These transgenic mice express a myc peptide tagged mutant (truncated) human DISC1 gene under the control of tetracycline operator (tetO). When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) to create a bitransgenic animal, tissue-specific transgene expression can be regulated with the tetracycline analog, doxycycline. When bred to a strain expressing rtTA or tTA in brain tissues (see Stock No. 003010, B6;CBA-Tg(Camk2a-tTA)1Mmay/J, for example), this mutant mouse strain may be useful in studies of psychiatric disorders such as schizophrenia. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Mikhail Pletnikov, Johns Hopkins University Description
These transgenic mice express a myc peptide-tagged, mutant (truncated) human DISC1 (disrupted in schizophrenia 1) gene under the control of tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator). The Donating Investigator reports that mice homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) to create a bitransgenic animal, tissue-specific mutant DISC1 transgene expression can be regulated with the tetracycline analog, doxycycline. When bred to a strain expressing rtTA or tTA in brain tissues (see Stock No. 003010, B6;CBA-Tg(Camk2a-tTA)1Mmay/J, for example), this mutant mouse strain may be useful in studies of psychiatric disorders such as schizophrenia.Development
The Tg(TRE-CMVhDISC1) transgene was designed with the myc peptide tagged mutant human DISC1 (disrupted in schizophrenia 1) gene, under the control of tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) sequence and a cytomegalovirus minimal promoter. This transgene was injected into fertilized B6;SJL mouse eggs. Founder line 1001 was subsequently established. The mice were then crossed to CBA and possibly C3H.
Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of tetO
View Strains carrying other alleles of tetO (80 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Tg(Camk2a-tTA)1Mmay/0 Tg(tetO-DISC1*)1001Plet/0
involves: C57BL/6 * CBA * SJL
- behavior/neurological phenotype
- abnormal social/conspecific interaction
- male mutants spend significantly less time in non-aggressive social interaction with other males (MGI Ref ID J:146881)
- increased aggression towards mice
- males display increased aggressiveness toward other males, with an increase in number of attacks (MGI Ref ID J:146881)
- abnormal spatial reference memory
- spatial memory in females is impaired in probe trials, with females spending less time in the target quadrant where the platform had previously been (MGI Ref ID J:146881)
- hyperactivity
- male mutants show higher degree of spontaneous locomotor activity than wild-type littermate controls during a 20-hour period (MGI Ref ID J:146881)
- nervous system phenotype
- abnormal lateral ventricle morphology
- volume is significantly increased, with no change in total brain volume (MGI Ref ID J:146881)
- abnormal neuron morphology
- cultured control neurons grow more complex neurite arbors than mutant neurons (for 20-100 um rings centered on neuron soma) (MGI Ref ID J:146881)
This mouse can be used to support research in many areas including:
Neurobiology Research
Behavioral and Learning Defects
Tet Expression System
tTA/rtTA Responsive Strains
Research Tools
Neurobiology Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Responsive Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(tetO-DISC1*)1001Plet | ||
|---|---|---|---|
| Allele Name | transgene insertion 1001, Mikhail Pletnikov | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Tg(TRE-CMVhDISC1); Tg(tetO/CMV-DISC1*)1001Plet; | ||
| Strain of Origin | C57BL/6 and SJL | ||
| Expressed Gene | DISC1, disrupted in schizophrenia 1, human | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | The transgene construct was designed with the myc peptide tagged mutant human DISC1 (disrupted in schizophrenia 1) gene, under the control of tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) sequence and a cytomegalovirus minimal promoter. Founder line 1001, which showed strong expression, was established. A second line, line 70, shows weak expression of human DISC1. [MGI Ref ID J:146881] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tTA), Melt Curve Analysis
Tg(teto-DISC1*)1001Plet, Fast MCA
Tg(teto-DISC1*)1001Plet, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Tg(tetO-DISC1*)1001Plet relatedPletnikov MV; Ayhan Y; Nikolskaia O; Xu Y; Ovanesov MV; Huang H; Mori S; Moran TH; Ross CA. 2008. Inducible expression of mutant human DISC1 in mice is associated with brain and behavioral abnormalities reminiscent of schizophrenia. Mol Psychiatry 13(2):173-86, 115. [PubMed: 17848917] [MGI Ref ID J:146881]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes or homozygotes. The Donating Investigator reports that homozygous mice are viable and fertile.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use
General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
Contact information
General inquiriesContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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