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| These transgenic mice express Cre recombinase under the control of the mouse Prdm1 (PR domain containing 1, with ZNF domain; Blimp1) promoter. Cre-mediated recombination is detected in 55-76% of primordial germ cells when this strain is crossed with Gt(ROSA)26-GFP reporter mice. These mice may be useful for generating tissue-specific targeted mutants for studies of development and germ cell fate. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
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- Health & husbandry
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Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Michel Nussenzweig, The Rockefeller University Description
These transgenic mice express Cre recombinase under the control of the mouse Prdm1 (PR domain containing 1, with ZNF domain; Blimp1) promoter. Cre-mediated recombination is detected in 55-76% of primordial germ cells when this strain is crossed with Gt(ROSA)26-GFP reporter mice. Expression is also seen in plasma cells. These mice may be useful for generating tissue-specific targeted mutants for studies of development and germ cell fate.Development
The exon 1 coding region of the targeted mouse gene was substituted with the first 100bp of the Cre sequence preceded by a nuclear localization sequence (NLS). Exon 2 was replaced by the remaining Cre sequence followed by a transcriptional stop cassette. The modified BAC vector was linearized and microinjected into pronuclei of B6CBAF1 zygotes. This strain was backcrossed to C57BL/6 four times by the donating investigator.
Related Strains
Strains carrying other alleles of Prdm1
008100 B6.129-Prdm1tm1Clme/J View Strains carrying other alleles of Prdm1 (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Prdm1-cre)1Masu | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, M Azim Surani | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Blimp1-Cre; Blimp1Cre; | ||
| Mutation Made By | M. Azim Surani, Gurdon Institute,University of Cambridge | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Prdm1, PR domain containing 1, with ZNF domain, mouse, laboratory | ||
| Driver Note | Prdm1 | ||
| Molecular Note | The coding region in exon 1 and 2 were replaced by an NLS and Cre cDNA sequence, followed by a transcriptional STOP cassette. [MGI Ref ID J:99994] | ||
| Gene Symbol and Name | Tg(Prdm1-cre)1Masu, transgene insertion 1, M Azim Surani | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Blimp1-Cre; Blimp1Cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Prdm1-cre), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Ohinata Y; Payer B; O'Carroll D; Ancelin K; Ono Y; Sano M; Barton SC; Obukhanych T; Nussenzweig M; Tarakhovsky A; Saitou M; Surani MA. 2005. Blimp1 is a critical determinant of the germ cell lineage in mice. Nature 436(7048):207-13. [PubMed: 15937476] [MGI Ref ID J:99994]
Additional References
Tg(Prdm1-cre)1Masu relatedHorsley V; O'Carroll D; Tooze R; Ohinata Y; Saitou M; Obukhanych T; Nussenzweig M; Tarakhovsky A; Fuchs E. 2006. Blimp1 defines a progenitor population that governs cellular input to the sebaceous gland. Cell 126(3):597-609. [PubMed: 16901790] [MGI Ref ID J:115995]
Robertson EJ; Charatsi I; Joyner CJ; Koonce CH; Morgan M; Islam A; Paterson C; Lejsek E; Arnold SJ; Kallies A; Nutt SL; Bikoff EK. 2007. Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice. Development 134(24):4335-45. [PubMed: 18039967] [MGI Ref ID J:129204]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes may be bred with wildtype mice.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
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Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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