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| These transgenic mice express the mutant human ubiquitin B, UBB+1, under the direction of the mouse Camk2a, calcium/calmodulin-dependent protein kinase II alpha, promoter. Hemizygous transgenic mice exhibit diminished ubiquitin-proteasome system activity with a resulting increase in ubiquitinated proteins in the cortex. Transgenic mice display impaired spatial learning. This mutant mouse strain may be useful in studies of neurodegenerative diseases such as Alzheimer's and Huntington disease. | ||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
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Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 30-MAY-09 Species laboratory mouse Generation N17+ (29-MAY-09)
Generation DefinitionsDonating Investigator F.W. Van Leeuwen, University Maastricht Description
These transgenic mice express the mutant human ubiquitin B, UBB+1, under the direction of the mouse Camk2a, calcium/calmodulin-dependent protein kinase II alpha, promoter. In hemizygotes, mutant transcript is expressed at 49% of the endogenous levels. The transgene is expressed in neurons of the cortex, hippocampus, amygdala and striatum. Hemizygous transgenic mice exhibit diminished ubiquitin-proteasome system activity with a resulting increase in ubiquitinated proteins in the cortex. Transgenic mice display a reduced synaptic plasticity in the hippocampus and impaired spatial learning. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical abnormalities. This mutant mouse strain may be useful in studies of neurodegenerative diseases such as Alzheimer's and Huntington disease.Development
A transgenic construct containing cDNA encoding the mutant human ubiquitin B, UBB+1, under the direction of the mouse Camk2a, calcium/calmodulin-dependent protein kinase II alpha, promoter was injected into fertilized C57BL/6 mouse eggs. Founder line 3413-1 was subsequently established. The mice were backcrossed to C57BL/6 for 16 generations before arriving at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Alzheimer's Disease Models
View Alzheimer's Disease Models (87 strains)
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002362 B6.129P2-Camk2atm1Sva/J 007574 B6.Cg-Tg(Camk2a-Crebbp*)1364Tabe/J 005855 B6.Cg-Tg(Camk2a-Prkaca)426Tabe/J 005359 B6.Cg-Tg(Camk2a-cre)T29-1Stl/J 007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 004995 C3H-Tg(Camk2a-Creb1/ESR1)3Sva/J 006575 C57BL/6-Camk2atm1Vyb/J 006579 C57BL/6-Tg(Camk2a-Bdnf)A9Stl/J 005087 C57BL/6-Tg(Camk2a-IDE)1Selk/J 005086 C57BL/6-Tg(Camk2a-MME)3Selk/J
Additional Web Information
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Visit the Huntington's Diease Resource site for helpful information on Huntington's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
Tg(Camk2a-UBB*)3413-1Fwvl/?
C57BL/6-Tg(Camk2a-UBB*)3413-1Fwvl
- behavior/neurological phenotype
- abnormal spatial reference memory (MGI Ref ID J:147938)
- 9-month old male mice exhibit poor performance in the Morris water maze task in probe trials done 1 week after training commenced; mice show no quadrant preference for the former location of the platform, in contrast to wild-type mice, and significantly larger distance to platform travel compared to wild-type
- impaired contextual conditioning behavior (MGI Ref ID J:147938)
- in a fear-conditioning paradigm, 9-month old mice exhibit significantly less freezing behavior on day 8 after training than wild-type littermates; however, cued-conditioning behavior is similar in transgenic and wild-type littermates
This mouse can be used to support research in many areas including:
Neurobiology Research
Alzheimer's Disease
Behavioral and Learning Defects
Huntington's disease
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Camk2a-UBB*)3413-1Fwvl | ||
|---|---|---|---|
| Allele Name | transgene insertion 3413-1, FW van Leeuwen | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | UBB+1 3413; UBB<+1> 3413; | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | UBB, ubiquitin B, human | ||
| Promoter | Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory | ||
| Molecular Note | A transgenic construct containing cDNA encoding the mutant human ubiquitin B, UBB+1 sequence (in the +1 reading frame), under the direction of the mouse Camk2a (calcium/calmodulin-dependent protein kinase II alpha) promoter was injected into fertilized C57BL/6 mouse eggs. Founder line 3413-1 with 13 copies of the transgene was subsequently established. mRNA levels for UBB+1 are 49% of endogenous Ubb mRNA levels. [MGI Ref ID J:147938] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Camk2a-UBB), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Fischer DF; van Dijk R; van Tijn P; Hobo B; Verhage MC; van der Schors RC; Li KW; van Minnen J; Hol EM; van Leeuwen FW. 2009. Long-term proteasome dysfunction in the mouse brain by expression of aberrant ubiquitin. Neurobiol Aging 30(6):847-63. [PubMed: 18760506] [MGI Ref ID J:147938]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375/MGL377
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator reports that homozygotes are viable and fertile. Mating System Noncarrier x Hemizygote (Female x Male) 30-MAY-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Camk2a-UBB)3413-1Fwvl
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Camk2a-UBB)3413-1Fwvl x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Camk2a-UBB)3413-1Fwvl
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Camk2a-UBB)3413-1Fwvl
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Camk2a-UBB)3413-1Fwvl x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Camk2a-UBB)3413-1Fwvl
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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