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| These transgenic mice express the mutant human ubiquitin B, UBB+1, under the direction of the mouse Camk2a, calcium/calmodulin-dependent protein kinase II alpha, promoter. Hemizygous transgenic mice exhibit diminished ubiquitin-proteasome system activity with a resulting increase in ubiquitinated proteins in the cortex. Transgenic mice display impaired spatial learning. This mutant mouse strain may be useful in studies of neurodegenerative diseases such as Alzheimer's and Huntington disease. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?pN1
Generation DefinitionsDonating Investigator F.W. Van Leeuwen, University Maastricht Description
These transgenic mice express the mutant human ubiquitin B, UBB+1, under the direction of the mouse Camk2a, calcium/calmodulin-dependent protein kinase II alpha, promoter. In hemizygotes, mutant transcript is expressed at 49% of the endogenous levels. The transgene is expressed in neurons of the cortex, hippocampus, amygdala and striatum. Hemizygous transgenic mice exhibit diminished ubiquitin-proteasome system activity with a resulting increase in ubiquitinated proteins in the cortex. Transgenic mice display a reduced synaptic plasticity in the hippocampus and impaired spatial learning. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical abnormalities. This mutant mouse strain may be useful in studies of neurodegenerative diseases such as Alzheimer's and Huntington disease.Development
A transgenic construct containing cDNA encoding the mutant human ubiquitin B, UBB+1, under the direction of the mouse Camk2a, calcium/calmodulin-dependent protein kinase II alpha, promoter was injected into fertilized C57BL/6 mouse eggs. Founder line 3413-1 was subsequently established. The mice were backcrossed to C57BL/6 for 16 generations before arriving at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Alzheimer's Disease Models
View Alzheimer's Disease Models (108 strains)
007708 B6.129-Gt(ROSA)26Sortm1(HD*103Q)Xwy/J 003454 B6.129-Htttm3Mem/J 003597 B6.129-Htttm4Mem/J 003598 B6.129-Htttm5Mem/J 016094 B6.129P2-Git2Gt(XG510)Byg/WeisJ 016522 B6.129P2-Htttm2Detl/100J 004595 B6.129P2-Htttm2Detl/150J 016521 B6.129P2-Htttm2Detl/50J 002688 B6.129S4-Htttm1Mem/J 006471 B6.Cg-Tg(HDexon1)61Gpb/J 008333 B6;129P2-Dldtm1Ptl/J 004360 B6;SJL-Tg(HD)63Aron/J 003627 B6C3-Tg(HD82Gln)81Dbo/J 002809 B6CBA-Tg(HDexon1)61Gpb/1J 002810 B6CBA-Tg(HDexon1)62Gpb/1J 006494 B6CBA-Tg(HDexon1)62Gpb/3J 016095 C.129P2(B6)-Git2Gt(XG510)Byg/WeisJ 007578 CBy.Cg-Tg(HDexon1)61Gpb/J 013732 FVB-Tg(NPEPPS)1Skar/J 004938 FVB-Tg(YAC128)53Hay/J 012630 FVB/N-Tg(GFAP-HTT*160Q)31Xjl/J 008197 FVB/N-Tg(HTT*97Q)IXwy/J 007247 FVB/N-Tg(YAC353G6)W7Hay/J 003640 FVB/NJ-Tg(YAC72)2511Hay/J 007749 STOCK Hap1tm1Xjl/J 003453 STOCK Htttm2Mem/J 003455 STOCK Htttm4Mem/J 003456 STOCK Htttm5Mem/J
016198 129S6.Cg-Tg(Camk2a-tTA)1Mmay/JlwsJ 002362 B6.129P2-Camk2atm1Sva/J 007574 B6.Cg-Tg(Camk2a-Crebbp*)1364Tabe/J 005855 B6.Cg-Tg(Camk2a-Prkaca)426Tabe/J 005359 B6.Cg-Tg(Camk2a-cre)T29-1Stl/J 007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 012362 B6;129S6-Tg(Camk2a-cre/ERT2)1Aibs/J 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 004995 C3H-Tg(Camk2a-Creb1/ESR1)3Sva/J 006575 C57BL/6-Camk2atm1Vyb/J 013760 C57BL/6-Tg(Camk2a-AIDPak)21Stl/J 006579 C57BL/6-Tg(Camk2a-Bdnf)A9Stl/J 005087 C57BL/6-Tg(Camk2a-IDE)1Selk/J 005086 C57BL/6-Tg(Camk2a-MME)3Selk/J 010712 C57BL/6-Tg(Camk2a-tTA)1Stl/J 008716 FVB/N-Tg(Myh6-AIP/PLN*)46Jded/J 015838 STOCK Tg(Camk2a-tTA)1Mmay Tg(tetO-ABL1*P242E*P249E)CPdav/J
Additional Web Information
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Visit the Huntington's Diease Resource site for helpful information on Huntington's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
Tg(Camk2a-UBB*)3413-1Fwvl/?
C57BL/6-Tg(Camk2a-UBB*)3413-1Fwvl
- behavior/neurological phenotype
- abnormal spatial reference memory
- 9-month old male mice exhibit poor performance in the Morris water maze task in probe trials done 1 week after training commenced; mice show no quadrant preference for the former location of the platform, in contrast to wild-type mice, and significantly larger distance to platform travel compared to wild-type (MGI Ref ID J:147938)
- impaired contextual conditioning behavior
- in a fear-conditioning paradigm, 9-month old mice exhibit significantly less freezing behavior on day 8 after training than wild-type littermates; however, cued-conditioning behavior is similar in transgenic and wild-type littermates (MGI Ref ID J:147938)
This mouse can be used to support research in many areas including:
Neurobiology Research
Alzheimer's Disease
Behavioral and Learning Defects
Huntington's disease
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Camk2a-UBB*)3413-1Fwvl | ||
|---|---|---|---|
| Allele Name | transgene insertion 3413-1, FW van Leeuwen | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | UBB+1 3413; UBB+1 3413; | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | UBB, ubiquitin B, human | ||
| Promoter | Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory | ||
| Molecular Note | A transgenic construct containing cDNA encoding the mutant human ubiquitin B, UBB+1 sequence (in the +1 reading frame), under the direction of the mouse Camk2a (calcium/calmodulin-dependent protein kinase II alpha) promoter was injected into fertilized C57BL/6 mouse eggs. Founder line 3413-1 with 13 copies of the transgene was subsequently established. mRNA levels for UBB+1 are 49% of endogenous Ubb mRNA levels. [MGI Ref ID J:147938] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Camk2a-UBB), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Fischer DF; van Dijk R; van Tijn P; Hobo B; Verhage MC; van der Schors RC; Li KW; van Minnen J; Hol EM; van Leeuwen FW. 2009. Long-term proteasome dysfunction in the mouse brain by expression of aberrant ubiquitin. Neurobiol Aging 30(6):847-63. [PubMed: 18760506] [MGI Ref ID J:147938]
Additional References
Tg(Camk2a-UBB*)3413-1Fwvl relatedde Pril R; Hobo B; van Tijn P; Roos RA; van Leeuwen FW; Fischer DF. 2010. Modest proteasomal inhibition by aberrant ubiquitin exacerbates aggregate formation in a Huntington disease mouse model. Mol Cell Neurosci 43(3):281-6. [PubMed: 20005957] [MGI Ref ID J:158445]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator reports that homozygotes are viable and fertile.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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