Strain Name:

C57BL/6-Tg(Lck-VIPR2)1Ejg/J

Stock Number:

008842

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Availability:

Cryopreserved - Ready for recovery

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These "VPAC2 R TG mice" (or VPAC2 Tg mice) have the murine lymphocyte protein tyrosine kinase (Lck) proximal promoter directing persistently high expression of huVPAC2R (human vasoactive intestinal peptide G-protein-coupled receptor 2 [VIPR2 primarily to CD4+ T cells at levels typically observed only after maximal TCR stimulation, and may be useful as a Th2-bias mouse model for studying innate and adaptive immunity, T cell and macrophage function, allergy and inflammation, and cytokine secretion, as well as immunopathologies related to Th1/Th2 cell ratio imbalance and neuroendocrine/neuroactive peptides in immune function.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Coisogenic; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Edward J Goetzl,   Univ of California Medical Center (UCSF)

Description
These "VPAC2 R TG mice" (or VPAC2R Tg mice) are viable and fertile, harboring the LCK-huVPAC2 transgene. This transgene has the murine lymphocyte protein tyrosine kinase (Lck) proximal promoter directing persistently high expression of huVPAC2R (human vasoactive intestinal peptide G-protein-coupled receptor 2 [VIPR2 or VIPR2]) primarily to CD4+ T cells (T-helper/inducer cells). As such, expression of huVPAC2R mRNA and protein is highest in spleen and thymus, but also observed in other tissues at much lower levels. The constitutive high huVPAC2R expression on CD4+ T cells is characteristic of levels usually observed only after maximal TCR stimulation. Transgenic mice have elevated blood IgE, IgG1, and eosinophil levels. The effector T cell phenotype is altered; with Th2-bias cytokine profiles (increased IL-4, IL-5, c-maf and junB) associated with altered hypersensitivity states (increased immediate-type hypersensitivity/decreased delayed-type hypersensitivity). Expression of huVPAC2R does not significantly alter the number or composition of splenic T cells. Although transgenic mice have more IL-4-secreting CD4+ T cells from splenic preparations compared with wildtype mice, the amount of IL-4 secreted per cell is not altered. These VPAC2 R TG mice may be useful as a Th2-bias mouse model for studying innate and adaptive immunity, T cell and macrophage function, allergy and inflammation, and cytokine secretion, as well as immunopathologies related to Th1/Th2 cell ratio imbalance and neuroendocrine/neuroactive peptides in immune function.

Development
The 4.6 kb LCK-huVPAC2 transgene was designed with a murine lymphocyte protein tyrosine kinase (Lck) proximal promoter and full-length human VPAC2R cDNA (huVPAC2 R or VIPR2; vasoactive intestinal peptide G-protein-coupled receptor 2). This transgene was microinjected into C57BL/6 oocytes, and a male founder animal (founder line unspecified) was bred to C57BL/6 females to establish the transgenic colony. This colony was then maintained by breeding high CD4+ T cell VPAC2R expressing transgenic mice together for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) to establish the colony.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Lck     (12 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(Lck-VIPR2)1Ejg/0

        C57BL/6-Tg(Lck-VIPR2)1Ejg
  • immune system phenotype
  • decreased interferon-gamma secretion
    • after TCR stimulation splenic CD4+ T cells produce less interferon-gamma (Ifng) than wild-type cells at 24 and 96 hours after stimulation   (MGI Ref ID J:127974)
  • decreased susceptibility to type IV hypersensitivity reaction
    • antigen-specific delayed-type hypersensitivity is depressed compared to controls indicated by reduced footpad swelling at 24 hours after immunization with NP OSu   (MGI Ref ID J:127974)
  • increased IgE level
    • serum levels are significantly higher than in wild-type   (MGI Ref ID J:127974)
    • levels of antigen-specific IgE during primary and secondary responses to immunization with TNP-KLH are significantly higher than in controls   (MGI Ref ID J:127974)
  • increased IgG1 level
    • serum levels are significantly higher than in wild-type   (MGI Ref ID J:127974)
    • levels of antigen-specific IgE during primary and secondary responses to immunization with TNP-KLH are marginally higher than in controls   (MGI Ref ID J:127974)
  • increased eosinophil cell number
    • serum levels of eosinophils are significantly higher than in wild-type   (MGI Ref ID J:127974)
  • increased interleukin-4 secretion
    • after TCR stimulation splenic CD4+ T cells produce significantly more IL4 than wild-type cells at 24 and 96 hours after stimulation   (MGI Ref ID J:127974)
  • increased interleukin-5 secretion
    • after TCR stimulation splenic CD4+ T cells produce significantly more IL5 than wild-type cells at 24 and 96 hours after stimulation   (MGI Ref ID J:127974)
  • increased susceptibility to type I hypersensitivity reaction
    • mice show greater immediated cutaneous hypersensitivity to antigen challenge indicated by significantly greater footpad swelling after 60 minutes than in controls   (MGI Ref ID J:127974)
    • much higher active cutaneous anaphylaxis reaction (swelling) is elicited in flank skin of secondarily immunized mice compared to controls at 30 minutes after TNP-CGG challenge   (MGI Ref ID J:127974)
  • hematopoietic system phenotype
  • increased IgE level
    • serum levels are significantly higher than in wild-type   (MGI Ref ID J:127974)
    • levels of antigen-specific IgE during primary and secondary responses to immunization with TNP-KLH are significantly higher than in controls   (MGI Ref ID J:127974)
  • increased IgG1 level
    • serum levels are significantly higher than in wild-type   (MGI Ref ID J:127974)
    • levels of antigen-specific IgE during primary and secondary responses to immunization with TNP-KLH are marginally higher than in controls   (MGI Ref ID J:127974)
  • increased eosinophil cell number
    • serum levels of eosinophils are significantly higher than in wild-type   (MGI Ref ID J:127974)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Growth Factors/Receptors/Cytokines

Cell Biology Research
Genes Regulating Growth and Proliferation
Signal Transduction

Developmental Biology Research
Internal/Organ Defects
      Lymphoid Tissue Defects
Lymphoid Tissue Defects

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Inflammation
Intracellular Signaling Molecules
Lymphoid Tissue Defects
T Cell Receptor Signaling Defects

Internal/Organ Research
Lymphoid Tissue Defects

Research Tools
Cancer Research
      T cell deficiency
      specific T cell deficiency
Immunology, Inflammation and Autoimmunity Research
      Macrophage Deficiency
      T cell deficiency
      genes regulating susceptibility to infectious disease and endotoxin
      production of T cell lines and hybridomas
      specific T cell deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Lck-VIPR2)1Ejg
Allele Name transgene insertion 1, Edward J Goetzl
Allele Type Transgenic (random, expressed)
Common Name(s) LCK-huVPAC2; LCK-huVPAC2R; huVPAC2 R;
Mutation Made By Edward Goetzl,   Univ of California Medical Center (UCSF)
Strain of OriginC57BL/6
Expressed Gene VIPR2, vasoactive intestinal peptide receptor 2, human
Promoter Lck, lymphocyte protein tyrosine kinase, mouse, laboratory
Molecular Note A murine lymphocyte protein tyrosine kinase (Lck) proximal promoter was used to drive the full-length human VPAC2R cDNA (huVPAC2 R or VIPR2; vasoactive intestinal peptide G-protein-coupled receptor 2). This transgene was microinjected into C57BL/6 oocytes, and a male founder animal was bred to C57BL/6 females to establish the line. [MGI Ref ID J:127974]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Lck-VIPR2), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Voice J; Donnelly S; Dorsam G; Dolganov G; Paul S; Goetzl EJ. 2004. c-Maf and JunB mediation of Th2 differentiation induced by the type 2 G protein-coupled receptor (VPAC2) for vasoactive intestinal peptide. J Immunol 172(12):7289-96. [PubMed: 15187104]  [MGI Ref ID J:90832]

Voice JK; Dorsam G; Lee H; Kong Y; Goetzl EJ. 2001. Allergic diathesis in transgenic mice with constitutive T cell expression of inducible vasoactive intestinal peptide receptor. FASEB J 15(13):2489-96. [PubMed: 11689474]  [MGI Ref ID J:127974]

Additional References

Tg(Lck-VIPR2)1Ejg related

Voice JK; Grinninger C; Kong Y; Bangale Y; Paul S; Goetzl EJ. 2003. Roles of vasoactive intestinal peptide (VIP) in the expression of different immune phenotypes by wild-type mice and T cell-targeted type II VIP receptor transgenic mice. J Immunol 170(1):308-14. [PubMed: 12496414]  [MGI Ref ID J:127285]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice. The donating investigator reports breeding homozygous mice together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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